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Detection of matrix metalloproteinase-11 in menstrual blood by enhanced chemiluminescence method / 法医学杂志
Journal of Forensic Medicine ; (6): 109-111, 2012.
Artigo em Chinês | WPRIM | ID: wpr-983722
ABSTRACT
OBJECTIVE@#To explore the forensic application value of detection of matrix metalloproteinase-11 (MMP-11) in menstrual blood by enhanced chemiluminescence method.@*METHODS@#Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated.@*RESULTS@#The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 microL sample was added, the mass concentration of protein was 1.329 microg/microL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4 degrees C for 20 months.@*CONCLUSION@#Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Saliva / Urina / Vagina / Manchas de Sangue / Biomarcadores / Western Blotting / Reprodutibilidade dos Testes / Sensibilidade e Especificidade / Metaloproteinase 11 da Matriz / Medicina Legal Limite: Feminino / Humanos Idioma: Chinês Revista: Journal of Forensic Medicine Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Saliva / Urina / Vagina / Manchas de Sangue / Biomarcadores / Western Blotting / Reprodutibilidade dos Testes / Sensibilidade e Especificidade / Metaloproteinase 11 da Matriz / Medicina Legal Limite: Feminino / Humanos Idioma: Chinês Revista: Journal of Forensic Medicine Ano de publicação: 2012 Tipo de documento: Artigo