Identification of sibling brothers using STR and Y-biallelic markers / 法医学杂志
Journal of Forensic Medicine
;
(6): 190-194, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-983735
ABSTRACT
OBJECTIVE@#To explore the methods for identification of sibling brothers with Y-STR locus mutation by detection of genetic markers on autosome and Y-biallelic.@*METHODS@#Goldeneye 20A and 18NC kit were used to genotyped the 35 STRs on autosome from two men. PowerPlex Y kit and Yfiler kit were used to genotyped the 16 STRs on Y chromosome full sibling index was calculated by ITO method. Twenty Y-biallelic markers were genotyped by fragment length discrepant allele specific PCR or general PCR.@*RESULTS@#Relationship of sibling brothers was found to have mutation of 2 loci on 16 Y-STR and the identical genetype of 20 Y-biallelic markers as well as a cumulative full sibling index of 4.3149 x 10(6) from 35 STRs on autosome.@*CONCLUSION@#In identification of paternal linage of Y-STR mutation, more genetic information can be acquired by detection of Y-biallelic markers including SNP and InDel.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo Genético
/
Marcadores Genéticos
/
Reação em Cadeia da Polimerase
/
Repetições de Microssatélites
/
Cromossomos Humanos Y
/
Irmãos
/
Alelos
/
Genética Forense
/
Loci Gênicos
/
Frequência do Gene
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo
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