A new method of identifying the peripheral blood and the menstrual blood / 法医学杂志
Journal of Forensic Medicine
;
(6): 359-361, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-983762
ABSTRACT
OBJECTIVE@#To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the peripheral blood and the menstrual blood.@*METHODS@#The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by electrophoretic technology.@*RESULTS@#Beta-spectrin (SPTB) as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 (MMP7) as one specific marker of menstrual blood and human beta defensin 1 (HBD1) as one specific marker of vaginal discharge were only found in the menstrual blood.@*CONCLUSION@#There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
RNA Mensageiro
/
Biomarcadores
/
Expressão Gênica
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Metaloproteinase 7 da Matriz
/
Perfilação da Expressão Gênica
/
Beta-Defensinas
/
Menstruação
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo
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