Method development and validation for determining 1,3-butadiene in human blood by gas chromatography-mass spectrometry and head-space gas chromatography / 法医学杂志
Journal of Forensic Medicine
;
(6): 110-115, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-983802
ABSTRACT
To develop a simple, validated method for identifying and quantifying 1,3-butadiene (BD) in human blood by gas chromatography-mass spectrometry (GC-MS) and head-space gas chromatography (HS-GC). BD was identified by GC-MS and HS-GC, and quantified by HS-GC. The method showed that BD had a good linearity from 50 to 500 microg/mL (r > 0.99). The limits of detection and quantification were 10 microg/mL and 50 microg/mL, respectively. Both the intra-day precision and inter-day precision were < 6.08%, and the accuracy was 96.98%-103.81%. The method was applied to an actual case, and the concentration of BD in the case was 242 microg/mL in human blood. This simple method is found to be useful for the routine forensic analysis of acute exposure to BD.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Solventes
/
Temperatura
/
Butadienos
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Toxicologia Forense
/
Intoxicação por Gás
/
Cromatografia Gasosa-Espectrometria de Massas
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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