Age estimation using content of sjTREC in human peripheral blood / 法医学杂志
Journal of Forensic Medicine
;
(6): 256-272, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-983831
ABSTRACT
OBJECTIVE@#To determine and verify the correlation formula of age estimation using the content of signal joint T-cell receptor excision DNA circle (sjTREC) in human peripheral blood and to discuss its application value in forensic biological practice.@*METHODS@#The samples of peripheral blood stains were collected from 30 healthy unrelated individuals whose ages were known. The DNAs were extracted from the samples stored at room temperature after 4 weeks. The content of sjTREC was measured by real-time fluorescent quantitative PCR technique, and the TATA box binding protein (TBP) was selected as reference genes. The age of each sample was predicted with the formula which was Age = -7.181 5 Y-42.458 +/- 9.42 (Y = dCtTBP-sjTREC), and the result was compared with the real age of each individual to determine the accuracy of the formula.@*RESULTS@#sjTREC and TBP gene were detectable in all 30 samples of peripheral blood. The contents of sjTREC in human peripheral blood showed a decreasing tendency with aging. The accuracy rate for the age estimation by this method was 76.67%.@*CONCLUSION@#The method for the age estimation with the content of sjTREC was simple, fast, sensitive, and good species specific with important potential application prospect.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Manchas de Sangue
/
Envelhecimento
/
DNA
/
Rearranjo Gênico do Linfócito T
/
Sensibilidade e Especificidade
/
Primers do DNA
/
Proteína de Ligação a TATA-Box
/
Genética Forense
/
Reação em Cadeia da Polimerase em Tempo Real
Limite:
Adolescente
/
Adulto
/
Idoso
/
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Lactente
/
Masculino
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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