Application of miniSTR Loci and Its Detection System for Degraded Materials in Forensic Medicine / 法医学杂志
Journal of Forensic Medicine
;
(6): 532-537, 2018.
Artigo
em Inglês
| WPRIM
| ID: wpr-984060
ABSTRACT
OBJECTIVES@#To establish multiplex system of 16 miniSTR loci, and explore its application value for the degraded materials in forensic medicine.@*METHODS@#The multiplex system of 16 miniSTR loci was established using a six-dye fluorescence labeling technology and its application value in forensic medicine was assessed.@*RESULTS@#A six-dye fluorescence labeling miniSTR amplification kit was developed, which enabled 15 autosomal STR loci, Amelogenin locus and DYS391 to be typed simultaneously. This method showed good specificity and could provide stable and accurate typing results with a sensitivity of 50 pg. This system also provided a good test result for the normal biological sample of actual cases.@*CONCLUSIONS@#The multiplex system of 16 miniSTR loci has application value for degraded and trace materials with the advantages of high sensitivity and database compatibility, which can be used for forensic casework.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Impressões Digitais de DNA
/
Primers do DNA
/
Repetições de Microssatélites
/
Amelogenina
/
Medicina Legal
Idioma:
Inglês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2018
Tipo de documento:
Artigo
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