Application of Multiple Displacement Amplification in Samples with Inhibitors / 法医学杂志
Journal of Forensic Medicine
;
(6): 342-345, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-984857
ABSTRACT
OBJECTIVES@#To explore the ability of inhibition resistibility of multiple displacement amplification (MDA) in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods (MBM) to purify sample.@*METHODS@#Different concentrations of hemoglobin and humid acid (HA) mixed with DNA samples and then divided the samples into MDA group, MBM group and control group. D3S1358 locus was amplified and detected by polyacrylamide gel electrophoresis detection system and AmpFℓSTR® Identifiler™ Plus Kit-capillary electrophoresis detection system.@*RESULTS@#When hemoglobin concentrations exceed 1 ng/μL or HA concentrations exceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.@*CONCLUSIONS@#MDA has the capability to remove the inhibition of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA
/
Hemoglobinas
/
Técnicas de Amplificação de Ácido Nucleico
/
Eletroforese em Gel de Poliacrilamida
/
Medicina Legal
/
Substâncias Húmicas
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2016
Tipo de documento:
Artigo
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