Application of COⅠ Gene Mini-barcoding in Species Identification of Hair / 法医学杂志

ABSTRACT

OBJECTIVES@#To identify the species of mammalian hair using COⅠ gene mini-barcoding technology.@*METHODS@#A pair of universal primers for mammalian COⅠ gene mini-barcoding were designed. The hair DNA samples of experimental animals from 11 species in 5 orders, mammalia was amplified by PCR technology with universal primers, and the PCR products were sequenced by bi-directional DNA and after the sequences splicing the results were deposited into the BOLD database to perform the homology comparison.@*RESULTS@#The DNA of hair from all experiment animal species could be totally amplified by the mini-barcoding universal primers designed in this study. The length of amplified fragment was 147 bp. The result of homology comparison in the database showed that the closest matching species were consistent with the experiment animal species. In addition to the matching degree of Panthera leo （98.99%）, all homology matching degree of the other experiment animals were 100%, and the intraspecific genetic distance of Panthera leo was 1%. The interspecific genetic distance was ten times more than the intraspecific genetic distance which could be used for species identification.@*CONCLUSIONS@#The COⅠ gene mini-barcode technology is established and can provide fast and accurate species identification for hair of mammals.