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Genetic Parameters of SNP Loci in Next Generation Sequencing Kits and Their Comparison / 法医学杂志
Journal of Forensic Medicine ; (6): 242-247, 2018.
Article em Zh | WPRIM | ID: wpr-984930
Biblioteca responsável: WPRO
ABSTRACT
OBJECTIVES@#To calculate genetic parameters of SNP loci in next generation sequencing kits, and to compare them with STR loci for establishing the conversion ratio between SNP and STR system effectiveness.@*METHODS@#Hardy-Weinberg equilibrium tests were performed in 101 SNP loci of next generation sequencing kits (ForenSeq™ DNA Signature Prep kit and Precision ID Identity Panel kit). The parameters of system effectiveness of SNP loci in the cases of personal identification, trios, duos, and alleged parents were calculated, which were compared with the genetic parameters of STR loci.@*RESULTS@#Except 2 loci without the data of genotype frequency, other 99 SNP loci conformed to Hardy-Weinberg equilibrium tests (P>0.05). In ForenSeq™ DNA Signature Prep kit, the CDP of 94 SNP loci was 1-1.152 1×10⁻³⁴, CPEtrio was 1-4.416 9×10⁻⁸, CPEduo was 1-8.483 7×10⁻⁵, and CPEAP was 1-1.222 7×10⁻¹². In Precision ID Identity kit, the CDP was 1-2.052 4×10⁻³³, CPEtrio was 1-8.709 3×10⁻⁸, CPEduo was 1-1.163 8×10⁻⁴, and CPEAP was 1-3.725 7×10⁻¹². In the cases of personal identification, trios, duos and alleged parents, the system effectiveness of 2.85, 4.51, 4.88 and 4.55 SNP loci was equal to that of 1 STR locus, respectively.@*CONCLUSIONS@#With high system effectiveness of SNP loci, the next generation sequencing kits is suitable for personal identification and paternity testing in forensic science.
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Texto completo: 1 Índice: WPRIM Assunto principal: Paternidade / Polimorfismo Genético / Kit de Reagentes para Diagnóstico / Impressões Digitais de DNA / Repetições de Microssatélites / Polimorfismo de Nucleotídeo Único / Genética Forense / Sequenciamento de Nucleotídeos em Larga Escala / Frequência do Gene / Genética Populacional Limite: Humans Idioma: Zh Revista: Journal of Forensic Medicine Ano de publicação: 2018 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Paternidade / Polimorfismo Genético / Kit de Reagentes para Diagnóstico / Impressões Digitais de DNA / Repetições de Microssatélites / Polimorfismo de Nucleotídeo Único / Genética Forense / Sequenciamento de Nucleotídeos em Larga Escala / Frequência do Gene / Genética Populacional Limite: Humans Idioma: Zh Revista: Journal of Forensic Medicine Ano de publicação: 2018 Tipo de documento: Article