Expressions of TGF-β1 and EⅢA-FN after Rat Skeletal Muscle Contusion and Wound Age Estimation / 法医学杂志
Journal of Forensic Medicine
;
(6): 154-159, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-984991
ABSTRACT
Objective To study the expressions of transforming growth factor-β1 (TGF-β1) and EⅢA-fibronectin (EⅢA-FN) at different time points of antemortem injury, antemortem injury postmortem expression and postmortem injury and to explore their application value in wound age estimation. Methods A model of rat skeletal muscle contusion was established. The rats were randomly divided into normal control group (n=5), antemortem contusion group (n=40), antemortem contusion postmortem expression group (n=110) and postmortem injury group (n=25). The expressions of TGF-β1 and EⅢA-FN after rat skeletal muscles antemortem contusion were detected with immunohistochemical staining. Expression changes of TGF-β1 and EⅢA-FN mRNA in each group were analyzed with real-time fluorescence quantitative PCR. Results Immunohistochemical staining results showed that a large number of polymorphonuclear leukocyte, mononuclear cells and fibroblastic cells showed a strong expression of TGF-β1 in wounded zones 12 h-14 d after antemortem contusion. EⅢA-FN was mainly distributed in the extracellular matrix, 3 to 7 d post-traumatic. Real-time fluorescence quantitative PCR results showed that TGF-β1 and EⅢA-FN mRNA in antemortem injury group reached the peak at 3 and 5 d post-traumatic respectively. The expressions of TGF-β1 and EⅢA-FN mRNA in antemortem contusion postmortem expression group peaked at 6 h and 12 h postmortem. The expression of TGF-β1 and EⅢA-FN mRNA in postmortem injury group 0.5-12 h postmortem was significantly lower than those of the normal control group and the antemortem contusion group. Conclusion TGF-β1 and EⅢA-FN might become a reference index for skeletal muscle wound age estimation.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Mudanças Depois da Morte
/
Biomarcadores
/
Distribuição Aleatória
/
Fibronectinas
/
Músculo Esquelético
/
Contusões
/
Fator de Crescimento Transformador beta1
/
Fibroblastos
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2019
Tipo de documento:
Artigo
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