Relationship between Changes of Pericyte Number and Wound Age during Repair of Skeletal Muscle Contusion in Mice / 法医学杂志
Journal of Forensic Medicine
;
(6): 267-272, 2019.
Artigo
em Inglês
| WPRIM
| ID: wpr-985005
ABSTRACT
Objective To observe the change pattern of pericyte number at different time periods after mice skeletal muscle contusion and discuss its role in wound age estimation. Methods A mice gastrocnemius muscle contusion model was established. The form and number changes of pericytes at 1, 3, 5, 7, 9, 14, and 28 d post-injury were detected by multiple immunofluorescence staining. Results Compared with the slender shape of pericytes in normal skeletal muscles, pericytes in the contusion area had increased volume, rounder form and a round nuclei. Part of pericytes were found to express satellite cell markers paired-box transcription factor (Pax7) or myoblast determination 1 (MyoD1). The changes of pericyte number in skeletal muscles after contusion were time-dependant, and showed unimodal distribution with the extension of wound age. In the central contusion area, the number of pericytes peaked at 5 d post-injury while in the peripheral contusion area, the number of pericytes peaked at 5 d and 7 d post-injury. Conclusion The number of pericytes in contusion area varies time-dependently after skeletal muscle contusion in mice and might be a reference index for muscle wound age estimation, and is involved in the repair and regeneration of skeletal muscle injury.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ratos Sprague-Dawley
/
Músculo Esquelético
/
Contusões
/
Pericitos
/
Modelos Animais de Doenças
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2019
Tipo de documento:
Artigo
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