Rapid Determination of Ammonia in Biological Samples by GC-MS Derivatization Method / 法医学杂志
Journal of Forensic Medicine
;
(6): 41-44, 2020.
Artigo
em Inglês
| WPRIM
| ID: wpr-985084
ABSTRACT
Objective To establish a qualitative and quantitative method to determine ammonia in biological samples by gas chromatography-mass spectrometry (GC-MS). Methods A heptafluorobutyryl chloride derivatization method was used. GC-MS was used for determination. The effects of different pH conditions, derivatization temperature, time and different extraction solvents on the test results were investigated. The pretreatment conditions were optimized. Results This method could accurately detect the ammonia content in blood, and the limit of detection was determined to be 0.1 μg/mL. The target component showed good linearity in the range of 0.5-200.0 μg/mL (R2=0.987 7). The relative standard deviation range of intra-day precision was 2.59%-3.88%. The relative standard deviation range of inter-day precision was 3.21%-3.76%. Conclusion The method showed good sensitivity, stability and specificity, therefore can be used for forensic toxicology analysis and clinical biochemical detection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Solventes
/
Reprodutibilidade dos Testes
/
Limite de Detecção
/
Amônia
/
Cromatografia Gasosa-Espectrometria de Massas
Idioma:
Inglês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2020
Tipo de documento:
Artigo
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