Effect of miR-581 on Autophagy of Ovarian Cancer SKOV3 Cells by Regulating FOXO1 / 肿瘤防治研究
Cancer Research on Prevention and Treatment
; (12): 403-407, 2022.
Article
em Zh
| WPRIM
| ID: wpr-986529
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore the effect and mechanism of miR-581 on the autophagy of ovarian cancer SKOV3 cells. Methods miR-581 mimics and miR-581 NC were transfected into SKOV3 cells, and the transfection efficiency was detected by qRT-PCR. After successful transfection, Western blot was used to detect autophagy-related proteins expression in SKOV3 cells. TargetScanHuman database predicted miR-581 target genes, and Western blot verified the role of miR-581 and target genes. Results Overexpression of miR-581 significantly inhibited the expression of autophagy-related proteins LC3 Ⅱ and Beclin1 (P < 0.01); miR-581 negatively regulated FOXO1 expression (P < 0.01) and FOXO1 promoted autophagy of SKOV3 cells (P < 0.01). Conclusion miR-581 affects the autophagy of ovarian cancer SKOV3 cells by regulating the expression of FOXO1.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Cancer Research on Prevention and Treatment
Ano de publicação:
2022
Tipo de documento:
Article