Expression of ITGAV in Non-small Cell Lung Cancer and Its Relationship with Radioresistance / 肿瘤防治研究

ABSTRACT

Objective To investigate the relationship between the expression of ITGAV and the radiosensitivity of NSCLC cells. Methods The expression of ITGAV in NSCLC and its relationship to the prognosis of patients who received radiotherapy were analyzed using bioinformatics methods. Differences in radiosensitivity between radio-resistant cells and parent cells were verified by clone formation experiment, and the protein expression of ITGAV was detected by Western blot. The transfection efficiency of si-ITGAV was determined by Western blot and qRT-PCR analyses. The best ITGAV interference sequence was selected to transfect A549R and H1299R cells. Clone formation experiment and flow cytometry were used to detect clone formation, apoptosis and cell cycle of A549R and H1299R cells. Results The expression of ITGAV in NSCLC tissues was significantly higher than that in normal tissues (P<0.05), and NSCLC patients with high ITGAV expression had poor prognosis. The clonogenic ability of the si-ITGAV group was significantly lower than that of the negative control group at 4, 6, 8Gy irradiation (all P<0.05). After 6 Gy irradiation, the apoptosis of the si-ITGAV group was increased (PH1299R<0.0001, PA549R=0.0002), the proportion of G2/M phase cells to A549-siITGAV and H1299R-siITGAV cells was higher than that in the negative control group (PH1299R<0.0001, PA549R=0.0007). Conclusion Interfering with ITGAV expression can increase the radiosensitivity of NSCLC.