The occurrence and progress of immune inflammatory response involved in gastroesophageal reflux disease / 中国医师进修杂志

ABSTRACT

Objective:To investigate the role of immune and inflammatory responses in the pathogenesis of gastroesophageal reflux disease (GERD).Methods:Fifteen patients with GERD who underwent biopsy and histopathology during painless gastroscopy in the digestive endoscopy center of the General Hospital of the PLA from December 2018 to September 2019 were analyzed retrospectively. They were divided into three groups 6 cases of non erosive reflux disease (NERD), 8 cases of reflux esophagitis (RE) and 1 case of RE with high-grade intraepithelial neoplasia (RE-HIN). HE staining was used to analyze the inflammatory reaction of each group. The expression of cyclooxygenase-2(COX-2), inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, myeloperoxidase (MPO), IL-4, nuclear factor kappa-B(NF-κB), IL-8, reactive oxygen species (ROS-1) were monitored by immunohistochemistry. Chi square test was used to analyze the positive rate of immune and inflammatory indexes in each group.Results:The three groups showed mild and moderate inflammatory cell infiltration, mostly lymphocyte infiltration, and basal cell hyperplasia in 3 cases. There was no significant difference between inflammatory cell infiltration, basal hyperplasia and inflammatory grade in NERD and RE( P>0.05). Immune and inflammatory factors COX-2(positive rateNERD 4/6, RE4/8, RE-HIN 1/1), iNOS(positive rateNERD 4/6, RE 3/8, RE-HIN 0), IL-1β(positive rateNERD 6/6, RE 7/8, RE-HIN 1/1), MPO(positive rateNERD 4/6, RE 7/8, RE-HIN 1/1), IL-4(positive rateNERD 3/6, RE 4/8, RE-HIN 0), IL-8(positive rateNERD 2/6, RE 6/8, RE-HIN 1/1), ROS-1(positive rateNERD 3/6, RE 1/8, RE-HIN 0) and signal pathway NF- κ B (positive rateNERD 4/6, RE 8/8, RE-HIN 1/1) were positive in three groups. The expressions of IL-1β, MPO and NF-κB were statistically significant among the three groups ( P<0.05). Conclusions:There is an inflammatory cascade mediated by immune inflammatory factors and mediators in GERD patients, and NF-κB signaling pathway is involved. It provides a basis for finding targets to block immune and inflammatory responses in the later stage to treat GERD.