Changes Produced by Different Concentrations of Inflammatory Cytokines in the Proliferation and Ciliary Movement of Human Respiratory Epithelial Cells
Journal of Rhinology
;
: 27-32, 1998.
Artigo
em Inglês
| WPRIM
| ID: wpr-99188
ABSTRACT
The aim of this study was to determine the effects of cytokines IL-1beta, TNF-alpha, and TGF-beta on the proliferation and ciliary beat frequency (CBF) of human nasal epithelial cells (HNECs) in vitro. Subcultured HNECs were incubated in a medium containing recombinant human (rh) cytokines rhIL-1beta rhTNF-alpha and rhTGF-beta at concentrations of 0.01 ng/ml, 0.1 ng/ml, 1 ng/ml, 10 ng/ml, and 100 ng/ml. After a two-day incubation with these cytokines, daily cell proliferation was measured by MTT assay for six days. The CBF was measured at concentrations of 1 ng/ml of rhIL-1beta 10 ng/ml of TNF-alpha and 1 ng/ml of TGF-beta solutions. While rhIL-1beta inhibited proliferation of HNECs in concentration-dependent and time-dependent manners, rhTNF-alpha stimulated HNEC growth at concentrations ranging from 0.01 ng/ml to 10 ng/ml in concentration-dependent and time-dependent manners. In contrast, rhTGF-beta inhibited HNEC growth irrespective of concentration and incubation time. The CBF of the human nasal ciliated epithelial cells increased after the addition of rhIL-1beta and rhTNF-alpha The CBF increased progressively for four hours after the addition of rhIL-1beta and rhTNF-alpha The increased CBF continued for 24 hours and decreased after two days. However, no variation of the CBF was observed after the addition of rhTGF-beta regardless of concentration or incubation time. The results of this study suggest that during acute inflammation, IL-1beta TNF-alpha and TGF-beta may have important roles in the repair and defense mechanism of the human nasal epithelium by regulating the proliferation and CBF of nasal epithelial cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Citocinas
/
Fator de Crescimento Transformador beta
/
Fator de Necrose Tumoral alfa
/
Proliferação de Células
/
Células Epiteliais
/
Inflamação
/
Mucosa Nasal
Limite:
Humanos
Idioma:
Inglês
Revista:
Journal of Rhinology
Ano de publicação:
1998
Tipo de documento:
Artigo
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