Study on molecular mechanism of Banxia Xiexin Decoction in intervention of gastric cancer cells pyroptosis based on multi database of bioinformatics / 中国医师杂志

ABSTRACT

Objective:Based on a variety of bioinformatics databases, the molecular mechanism of Banxia Xiexin Decoction (BXXXD) in the intervention of gastric cancer cells pyroptosis was investigated, providing new ideas for the survival and prognosis of gastric cancer patients.Methods:Bioinformatics databases used in this study include Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Pubchem, String, Genecard, Gene Expression Omnibus (GEO), and The Cancer Genome Atlas (TCGA), etc. Firstly, the common targets of " Banxia Xiexin Decoction→gastric cancer→pyroptosis" was obtained by the network pharmacology method of traditional Chinese medicine compound, and its Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were analyzed. Secondly, the difference of gastric cancer chip was analyzed by GEO database, and the differentially expressed genes (DEGs) volcano map and heat map were drawn. The common target of " Banxia Xiexin Decoction→Gastric cancer DEGs" was obtained by using Venn Diagram. The information of clinical gastric cancer patients was obtained through the TCGA database to compare the expression levels of relevant targets between the normal group and the gastric cancer group, and to analyze the relationship between the expression levels of each target and the clinicopathological characteristics of patients with gastric cancer.Results:There were 29 targets of Banxia Xiexin Decoction in the intervention of gastric cancer cells pyroptosis, which play a role in multi-biological processes, multi-cellular components, multi-molecular functions and multiple KEGG signaling pathways. The most important may be that in the cytoplasm, proteins of molecular targets bind to each other and positively regulate the biological processes of transcription pathways through the neural active ligand-receptor interaction signaling pathway. Further study showed that cholecystokinin B receptor (CCKBR), ATPase H + /K + transporting subunit alpha (ATP4A), ATP4B were the three common targets of " Banxia Xiexin Decoction → gastric cancer DEGs" . The expression of molecular targets CCKBR, ATP4A and ATP4B between normal group and gastric cancer group had statistical significance (all P<0.05). The expression level of CCKBR was correlated with age, T stage and M stage (all P<0.05), the expression level of ATP4A was correlated with age and T stage (all P<0.05), and the expression level of ATP4B was correlated with histological stage, T stage and N stage (all P<0.05). Conclusions:By using bioinformatics method, the relevant targets of Banxia Xiexin Decoction in the intervention of gastric cancer cells pyroptosis were obtained through a variety of databases, and the relationship between the targets and the clinicopathological characteristics of gastric cancer was preliminatively discussed, which provided a new research method for the intervention of traditional Chinese medicine in tumor diseases.