Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046
International Journal of Oral Biology
;
: 79-82, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-9938
ABSTRACT
The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strain-specificity of the PCR primers, Pig28-F1/Pig28-R1, was confirmed with 9 strains of P. intermedia and 25 strains (15 species) of Prevotella species. The detection limit of the PCR primers was 2 pg of the purified genomic DNA of P. intermedia ATCC 49046. These PCR primers were found to be useful for identifying P. intermedia ATCC 49046, particularly for determining the authenticity of the strain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Entorses e Distensões
/
DNA
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Prevotella intermedia
/
Prevotella
/
Limite de Detecção
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
International Journal of Oral Biology
Ano de publicação:
2011
Tipo de documento:
Artigo
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