Epidemiological investigation of Lyme disease in animals in Xinjiang Uygur Autonomous Region,China / 中国生物制品学杂志

ABSTRACT

@#ObjectiveTo investigate the epidemiology of Lyme disease and analyze the genotypes of Borrelia burgdorferi in five kinds of host animals of ticks in four cities of Xinjiang Uygur Autonomous Region,China.MethodsA total of 814 serum samples were collected from cattle,sheep,goats,horses and dogs in Urumqi,Ili,Changji and Kashgar Cities,and determined for IgG antibody against Lyme disease. A total of 135 ticks in the above-mentioned cities were collected,from which DNAs were extracted and used as template for amplification of the 5S-23S rRNA spacer fragments of B. burgdorferi by nested PCR. The positive samples were sequenced,and the results were compared with those of 5S-23S rRNA spacer of B. burgdorferi reported in GenBank by BLAST,and a phylogenetic tree was constructed by MEGA X software.ResultsThe IgG antibody positive rates against Lyme disease in Urumqi,Ili,Changji and Kashgar Cities were 23. 6%,2. 4%,2. 7% and 0 respectively,which showed significant difference（χ2= 48. 481,P < 0. 001）. However,the positive rates in cattle,sheep,goats,dogs and horses were 1. 1%,4. 4%,18. 7%,60. 5% and 0 respectively,which showed sig-nificant difference（χ2= 129. 03,P <0. 001）. Of the 135 tick DNA samples,24 were positive for B.burgdorferi,indicating a carrier rate of 17. 78%. Phylogenetic analysis showed that the genotypes B.garinii accounted for 75%,while B.afzelii accounted for 16. 67%,and B.burgdorferi accounted for 8. 33%.ConclusionThere are Lyme diseases in cattle,sheep,goats and dogs in Urumqi,Kashgar and Ili Cities of Xinjiang Uygur Autonomous Region,China,of which the major genotype is B.garinii. The study provides a scientific basis for prevention and control of Lyme disease in Xinjiang.