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Praksis ; 3:96-112, 2021.
Article in Portuguese | Scopus | ID: covidwho-1444652


The subject of the article is about face-To-face and distance learning, which will be a challenge for teachers in returning to classes after the coronavirus pandemiciBased on this scenario, the study aims to reflect on the return to classes and possible teaching trends in elementary schools in the municipality of Canoas in the state of Rio Grande do SuliThe methodological approach of this research is qualitative and is characterized as a literature review from books and scientific articles from the last 5 yearsiThe theoretical framework of the research is supported by authors such as Moran, 2015), Förh, 2019), Santana and Sales, 2020), Horn and Staker, 2015)iThe results show that the Covid-19 pandemic accelerated the innovation processes in municipal schools of basic education, modifying the means of teaching and learningiWe understand that in this new paradigm digital education emerges with an emphasis on digital, personalized and collective literacy: hybrid education. © 2021 UNIVERSIDADE FEEVALE . All Rights Reserved.

J Virol Methods ; 293: 114131, 2021 07.
Article in English | MEDLINE | ID: covidwho-1157565


The World Health Organization (WHO) has declared a pandemic of COVID-19, the disease caused by the recently described SARS-CoV-2. The relevance and importance of mass diagnosis in order to find the asymptomatic individuals is widely recognized as a mandatory tool to reinforce the control measures for monitoring virus circulation and reduce the spreading of SARS-CoV-2. Here, we described quickness and cheaper strategies of direct RT-qPCR (in the absence of RNA isolation) and compared the results to those obtained using standard RNA isolation procedure. The tests varied using pure, diluted samples, combined with Proteinase K (PK) or Lysis Buffer. Our findings showed consistently that PK pre-treated samples in the absence of RNA extraction procedures presents similar results to those obtained by standard RNA isolation procedures. On average, 16 samples extracted with the MagMAX™ CORE Kit, take around 2 h, costing an average of USD 5, the pre-treatment of samples using PK, on the other hand, would cut the value to less than USD 0.30 and reduce the time of procedure in more than 1 ½ hours. The present study suggests the use of PK treatment instead of RNA isolation in order to reduce costs and time in processing samples for molecular diagnosis of SARS-CoV-2.

COVID-19 Nucleic Acid Testing/methods , COVID-19/diagnosis , Endopeptidase K/pharmacology , RNA, Viral/analysis , SARS-CoV-2/isolation & purification , COVID-19 Nucleic Acid Testing/economics , Humans , SARS-CoV-2/genetics