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Talanta ; 252, 2023.
Article in English | Web of Science | ID: covidwho-2069714


Since the last century, animal viruses have posed great threats to the health of humans and the farming industry. Therefore, virus control is of great urgency, and regular, timely, and accurate detection is essential to it. Here, we designed a rapid on-site visual data-sharing detection method for the Newcastle disease virus with smartphone recognition-based immune microparticles. The detection method we developed includes three major modules: preparation of virus detection vectors, sample detection, and smartphone image analysis with data upload. First, the hydrogel microparticles containing active carboxyl were manufactured, which coated nucleocapsid protein of NDV. Then, HRP enzyme-labeled anti-NP nanobody was used to compete with the NDV antibody in the serum for color reaction. Then the rough detection results were visible to the human eyes according to the different shades of color of the hydrogel microparticles. Next, the smartphone application was used to analyze the image to determine the accurate detection results according to the gray value of the hydrogel microparticles. Meanwhile, the result was automatically uploaded to the homemade cloud system. The total detection time was less than 50 min, even without trained personnel, which is shorter than conventional detection methods. According to experimental results, this detection method has high sensitivity and accuracy. And especially, it uploads the detection information via a cloud platform to realize data sharing, which plays an early warning function. We anticipate that this rapid on-site visual data-sharing detection method can promote the development of virus selfchecking at home.

Chinese Journal of Biologicals ; 34(6):699-703, 2021.
Article in Chinese | EMBASE | ID: covidwho-1894085


Objective To explore the application and safety of apheresis technology in collection of Coronavirus Disease 2019 (COVID-19) convalescent plasma (CP), and to analyze the quality characteristics of the plasma. Methods The general data of COVID-19 convalescent plasma (CP) donors, including gender, age, date of discharge or release from medical isolation, were collected based on informed consent. After physical examination, the CP was collected by apheresis technology with plasma separator, inactivated with methylene blue, and determined for severe acute respiratory symptom Coronavirus 2 (SARS-CoV-2) nucleic acid and specific antibody (RBD-IgG) against SARS-CoV-2. Results The collection process went well, and no serious adverse events related to plasma collection were reported during or after the collection. The average age of COVID-19 CP donors was 38 years (n = 933). The distributions of blood groups A, B, AB and 0 in RhD (+) COVID-19 CP were 33. 4%, 29. 2%, 10% and 27. 2% respectively. The plasma donation date was 18 d from the discharge date in average. All the test results of SARS-CoV-2 nucleic acid in CP were negative, while the proportion of plasma samples at SARS-CoV-2 antibody titer of more than 1: 160 was 92. 60%. Conclusion Apheresis technology was safe and reliable. The COVID-19 CP contained high titer antibody. Large-scale collection and preparation of inactivated plasma against SARS-CoV-2 played an important role in the treatment of COVID-19.

IOP Conf. Ser. Earth Environ. Sci. ; 571, 2020.
Article in English | Scopus | ID: covidwho-990505


Under the influence of the COVID-19, consumers pay more and more attention to the safety and health of the air in the car. As the core component of the air purification in the car, the performance of the cabin air filter is a hot topic for vehicle enterprises and parts enterprises. This paper introduces the inspection and data analysis of cabin air filter products in China by China Automotive Technology And Research Center, which includes the product geographical distribution, brand compliance rate, the compliance rate of different testing items. Based on the analysis of fractional efficiency of 0.3 µm, 2.5 µm and 10 µm particle sizes and particle sizes distribution in the atmosphere, this paper puts forward the view that it is necessary to pay more attention to the fractional efficiency of particulates which below 1 µm. In addition, the existing problems of cabin air filter are studied by data and its development direction is prospected. © Published under licence by IOP Publishing Ltd.