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Indian Journal of Medical Microbiology ; 39:S60-S61, 2021.
Article in English | EMBASE | ID: covidwho-1734474


Background:There are more than 350 RT-PCR COVID-19 testing kits commercially available but these kits has not been evaluated for pooled sample testing. Thus, this study was planned to compare and evaluate seven commercially availa- ble kits for pooled samples testing. Methods:Diagnostic accuracy of (1) TRUPCR SARS-CoV-2 Kit (Black Bio, India) (2) TaqPath RT-PCR COVID-19 Kit (Thermo Fisher, USA) (3) Allplex 2019-nCOV Assay (See gene, Korea), (4) Patho detect COVID-19 PCR kit (My Lab, India) (5) LabGun COVID-19 RT-PCR Kit (Lab Genomics, Korea) (6) Fosun COVID-19 RT-PCR detection kit (Fosun Ltd, China) (7) Real time Fluorescent RT PCR kit for SARS CoV – 2 (BGI, china) was evaluated on pre characterised 40 positive and 10 nega- tive COVID-19 sample pools. Results:All seven kits detected all sample pool with low Ct value (<30). While testing weak positive pooled samples with high Ct value (>30);TRUPCR Kit, TaqPath Kit, Allplex Assay and BGI RT PCR kit showed 100% sensitivity, specificity and accuracy. However;Fosun kit, LabGun Kit and Patho detect kit could detect only 90%, 85% and 75% of weak positive samples respectively. [Formula presented] Conclusions:We conclude that all seven commercially available RT-PCR kits included in this study can be used for routine molecular diagnosis of COVID-19. While perform- ing pooled sample testing it might be advisable to use those kits that performed best regarding the positive iden- tification in samples pool i.e. TRUPCR SARS-CoV-2 Kit, TaqPath RT-PCR COVID-19 Kit, Allplex 2019-nCOV Assay and BGI Real time RT PCR kit for detecting SARS CoV – 2.