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1.
ACS Omega ; 7(17): 15106-15112, 2022 May 03.
Article in English | MEDLINE | ID: mdl-35572749

ABSTRACT

Petroleum cokes prepared from naphthenic crude oil differ significantly in terms of the oxygen content and hydrogen/carbon (H/C) ratio, which mainly depend on the different coking temperatures. Thermogravimetric-differential scanning calorimetry was applied to study the heat release and combustion weight loss of petroleum cokes prepared at 350 and 500 °C, respectively. The effect of different coke formation temperatures on the combustion properties of the coke formed during air injection in situ combustion (ISC) was also investigated. The results showed that the petroleum coke formed under oxygen exhibited an H/C ratio of 0.895 and an O/C ratio of 0.109 at 350 °C and an H/C ratio of 0.395 and an O/C ratio of 0.054 at 500 °C. As the temperature rises, the hydrogen atoms on the petroleum coke molecules intensify to separate and form water molecules and thus giving off heat. It can be further inferred that under the combustion temperature of air injection ISC, the coke at 350 °C can release more heat in the lower combustion temperature range, and the combustion weight loss is faster; however, the formation temperature continues to rise due to combustion at 500 °C, coke begins to release massive heat, and the combustion weight loss is as high as 97.95%. The combustion residuals of both temperature cokes and the residual solid content of the formation after combustion in porous media are both little, which can be used as fire flooding fuels at different formation temperatures to provide heat energy for oil displacement.

2.
Molecules ; 27(9)2022 Apr 24.
Article in English | MEDLINE | ID: mdl-35566090

ABSTRACT

Fatty acid synthase (FASN) is highly expressed in multiple types of human cancers and is recognized as one of the targets for treating cancer metastasis. α-Linolenic acid is an omega-3 essential fatty acid and it possesses various biological activities. The present study was designed to reveal the effects of α-linolenic acid on osteosarcoma and to reveal whether the mechanism of α-linolenic acid in anticancer activity may be related to FASN inhibition. The cytotoxicity of α-linolenic acid was assessed in osteosarcoma MG63, 143B, and U2OS cells. Cell viability was detected by the MTT assay. The protein expression level was detected by western blotting. Flow cytometry, Annexin V/propidium iodide dual staining, and Hoechst 33258 staining were performed to assess the apoptotic effects. Wound healing assay was applied to detect the inhibitory effect of α-linolenic acid on osteosarcoma cells migration. The results showed that α-linolenic acid downregulated FASN expression. α-Linolenic acid inhibited osteosarcoma cell proliferation and migration in a dose-dependent manner. In addition, α-linolenic acid regulated endoplasmic reticulum transmembrane receptors and signal protein expression in osteosarcoma cells. The findings of the present study suggested that α-linolenic acid suppresses osteosarcoma cell proliferation and metastasis by inhibiting FASN expression, which provides a basis as a potential target for osteosarcoma treatment.

3.
RSC Adv ; 8(63): 36090-36095, 2018 Oct 22.
Article in English | MEDLINE | ID: mdl-35558467

ABSTRACT

In this work, a novel strategy for synthesizing carbon dots (CDs) with a quantum yield of approximately 15.36% has been established by employing a bathroom lamp as a light source. Compared with other current protocols, the method described here displayed various advantages such as environmentally friendly manipulations and low power and cost. Subsequently, we applied the CDs as a fluorescence probe for the detection of nimesulide (Nim) firstly under the optimal conditions. A linear relationship between ln(F 0/F) and the concentration of Nim was obtained in the range from 0.5 µM to 75 µM with a detection limit of 100 nM. In addition, the as-prepared CDs showed excellent biocompatibility and were applied for cell imaging, which presented great potential applications in cell imaging.

4.
RSC Adv ; 8(66): 37573-37580, 2018 Nov 07.
Article in English | MEDLINE | ID: mdl-35558593

ABSTRACT

To find new pesticides for agricultural use, 133 fungal strains were isolated from coastal marine habitats, from which 37 independent isolates were identified, belonging to 20 genera in nine orders, and the diversity of the isolated fungi were discussed. The phytotoxicity and anti-phytopathogenic fungal and bacterial activities of these 37 extracts, and two previously isolated fungal extracts were evaluated, displaying different levels of bioactivity. Based on the bioactive and chemical screening, an Alternaria sp. (P8) strain, which showed prominent bioactivity and contained abundant secondary metabolites was selected for further chemical investigation; one new compound, a benzopyranone (1), and seven known compounds (2-8) were obtained. Their structures were determined by analysing extensive NMR spectroscopic data and ECD comparisons. Compounds 1, 2, and 6-8 showed obvious phytotoxicity, especially against amaranth, and compound 1 also showed potent antifungal activity toward Alternaria brassicicola. To the best of our knowledge, this is the first report of the phytotoxicity of marine-derived fungi and their secondary metabolites. These studies should provide the foundation for future research into the use of such fungal extracts to combat weeds and diseases in agriculture.

5.
Biochem Biophys Res Commun ; 613: 107-112, 2022 Apr 25.
Article in English | MEDLINE | ID: mdl-35550196

ABSTRACT

OBJECTIVE: We established a mouse cataract model by irradiating Grx2 knockout (KO) and knock-in (KI) genetically modified mice with UVB to explore the protective mechanism of Grx2 against UVB lens damage. METHODS: After irradiating Grx2 KO and Grx2 KI mice with UVB lamps, we observed and recorded the general physiological conditions and lens opacity of the mice. The crystalline grading system of the University of Oxford was used to classify the opacity of the lens. Lens reactive oxygen species (ROS) contents were detected using a microplate reader, western blot, and enzyme-linked immunosorbent assay (ELISA) to detect antioxidant and antioxidant enzyme contents. Statistical analysis of the recorded data was performed by using SPSS 19.0 software. RESULTS: After UVB irradiation, the weight of Grx2 KO mice was slightly lower than that of wild-type (WT) mice of the same age. Compared to WT mice, the lens opacity of Grx2 KO mice appeared earlier, the nucleus density of the lens increased, and the opacity increased in the first week after UVB irradiation. Meanwhile, the lenses of Grx2 KI mice remained transparent. The experiment showed that the content of ROS increased, the level of glutathione (GSH) decreased, the content of 8-OHdG increased, and the expression of BCL2 decreased after UVB irradiation. Compared to WT mice, these changes were more significant in Grx2 KO mice. CONCLUSION: This experiment found that knocking out the Grx2 gene accelerated the occurrence and development of UVB-induced cataracts in mice and that Grx2 plays an important role in the oxidative damage caused by UVB radiation by repairing the antioxidant enzymes of the lens. This study provides a new animal model and research ideas for the study of cataract pathogenesis.

6.
RSC Adv ; 8(41): 22986-22990, 2018 Jun 21.
Article in English | MEDLINE | ID: mdl-35540117

ABSTRACT

The alkali free surfactant-polymer flooding system with ultralow interfacial tension is a challenge in enhanced oil recovery at present. A novel alkali free binary flooding system of a biobased zwitterionic surfactant and hydrophobically associating polymer with ultralow interfacial tension at a low surfactant dosage was studied in this paper.

7.
RSC Adv ; 9(66): 38568-38575, 2019 Nov 25.
Article in English | MEDLINE | ID: mdl-35540185

ABSTRACT

In this study, dual-emission carbon dots (D-CDs) are synthesized via a simple one-step solvothermal treatment of red tea. The obtained D-CDs are characterized by XPS, IR, TEM, XRD, fluorescence and UV-vis spectroscopy techniques. It is found that D-CDs present a strong red fluorescence emission peak at 671 nm and weak blue fluorescence emission peak at 478 nm under the excitation wavelength of 410 nm. The unique dual-emission properties of D-CDs provide great opportunities in ratiometric fluorescence sensing applications. The results show that Cu2+ ions can quench the fluorescence of the red emission band of D-CDs effectively, resulting in the disappearance of red fluorescence ultimately. Upon the addition of Al3+ ions, the fluorescence of blue emission band at 478 nm grows apparently, and the fluorescence color transforms gradually from red to orange, then to yellow-green. Based on these findings, a novel ratiometric fluorescence and colorimetric dual mode nanosensor is developed for simultaneous detection of Cu2+ and Al3+ ions. Regarding Cu2+ ions, the fluorescent detection linear range is 0.1-50 µM with detection limit of 0.1 µM, and the colorimetric detection limit is estimated as 25 µM. With regard to Al3+ ions, the fluorescent detection linear range is 0-20 µM and 25-100 µM with detection limit of 0.5 µM, and the colorimetric detection limit is 20 µM. Furthermore, the fluorescence response mechanisms of Cu2+ and Al3+ ions were discussed detailed. To the best of our current knowledge, this will be the first research work on the simultaneous determination of Cu2+ and Al3+ using D-CDs as fluorescent probes.

8.
Redox Biol ; 53: 102326, 2022 Apr 30.
Article in English | MEDLINE | ID: mdl-35525025

ABSTRACT

The nonunion following a fracture is associated with severe patient morbidity and economic consequences. Currently, accumulating studies are focusing on the importance of macrophages during fracture repair. However, details regarding the process by which macrophages facilitate endochondral ossification (EO) are largely unknown. In this study, we present evidence that apoptotic chondrocytes (ACs) are not inert corpses awaiting removal, but positively modulate the osteoinductive ability of macrophages. In vivo experiments revealed that fatty acid (FA) metabolic processes up-regulated following EO. In vitro studies further uncovered that FAs derived from ACs are taken up by macrophages mainly through macrophage scavenger receptor 1 (MSR1). Then, our functional experiments confirmed that these exogenous FAs subsequently activate peroxisome proliferator-activated receptor α (PPARα), which further facilitates lipid droplets generation and fatty acid oxidation (FAO). Mechanistically, elevated FAO is involved in up-regulating the osteoinductive effect by generating BMP7 and NAD+/SIRT1/EZH2 axis epigenetically controls BMP7 expression in macrophages cultured with ACs culture medium. Our findings advanced the concept that ACs could promote bone regeneration by regulating metabolic and function reprogram in macrophages and identified macrophage MSR1 represents a valuable target for fracture treatments.

9.
Front Genet ; 13: 818241, 2022.
Article in English | MEDLINE | ID: mdl-35518361

ABSTRACT

Objectives: Some genetic causes of heterotaxy have been identified in a small number of heterotaxy familial cases or animal models. However, knowledge on the genetic causes of heterotaxy in the fetal population remains scarce. Here, we aimed to investigate the clinical characteristics and genetic spectrum of a fetal cohort with heterotaxy. Methods: We retrospectively investigated all fetuses with a prenatal diagnosis of heterotaxy at a single center between October 2015 and November 2020. These cases were studied using the genetic testing data acquired from a combination of copy number variation sequencing (CNV-seq) and whole-exome sequencing (WES), and their clinical phenotypes were also reviewed. Result: A total of 72 fetuses diagnosed with heterotaxy and complete clinical and genetic results were enrolled in our research. Of the 72 fetuses, 18 (25%) and 54 (75%) had left and right isomerism, respectively. Consistent with the results of a previous study, intracardiac anomalies were more severe in patients with right atrial isomerism than in those with left atrial isomerism (LAI) and mainly manifested as atrial situs inversus, bilateral right atrial appendages, abnormal pulmonary venous connection, single ventricles or single atria, and pulmonary stenosis or atresia. In 18 fetuses diagnosed with LAI, the main intracardiac anomalies were bilateral left atrial appendages. Of the 72 fetuses that underwent CNV-seq and WES, 11 (15.3%) had positive genetic results, eight had definitive pathogenic variants, and three had likely pathogenic variants. The diagnostic genetic variant rate identified using WES was 11.1% (8/72), in which primary ciliary dyskinesia (PCD)-associated gene mutations (CCDC40, CCDC114, DNAH5, DNAH11, and ARMC4) accounted for the vast majority (n = 5). Other diagnostic genetic variants, such as KMT2D and FOXC1, have been rarely reported in heterotaxy cases, although they have been verified to play roles in congenital heart disease. Conclusion: Thus, diagnostic genetic variants contributed to a substantial fraction in the etiology of fetal heterotaxy. PCD mutations accounted for approximately 6.9% of heterotaxy cases in our fetal cohort. WES was identified as an effective tool to detect genetic causes prenatally in heterotaxy patients.

10.
RSC Adv ; 9(12): 6890-6897, 2019 Feb 22.
Article in English | MEDLINE | ID: mdl-35518459

ABSTRACT

In this study, polypyrrole nanofiber (PPyNF)/NiO x composites were synthesized by a simple and fast microwave method. The samples were characterized using differential scanning calorimetry and thermal gravimetric analysis (DSC/TGA), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Furthermore, the synthesized PPyNF/NiO x nanocomposites were electrochemically characterized using galvanostatic charge-discharge, cyclic voltammetry and electrochemical impedance spectroscopy (EIS) techniques. They showed the highest specific capacitance of 657 F g-1 at 0.5 A g-1, demonstrating their potential application in supercapacitors.

11.
BMJ Open ; 12(5): e049516, 2022 May 03.
Article in English | MEDLINE | ID: mdl-35504636

ABSTRACT

OBJECTIVE: Adverse health effects of fine particles (particulate matter2.5) have been well documented by a series of studies. However, evidences on the impacts of black carbon (BC) or elemental carbon (EC) on health are limited. The objectives were (1) to explored the effects of BC and EC on cardiovascular and respiratory morbidity and mortality, and (2) to verified the reliability of the meta-analysis by drawing p value plots. DESIGN: The systematic review and meta-analysis using adapted Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach and p value plots approach. DATA SOURCES: PubMed, Embase and Web of Science were searched from inception to 19 July 2021. ELIGIBILITY CRITERIA FOR SELECTING STUDIES: Time series, case cross-over and cohort studies that evaluated the associations between BC/EC on cardiovascular or respiratory morbidity or mortality were included. DATA EXTRACTION AND SYNTHESIS: Two reviewers independently selected studies, extracted data and assessed risk of bias. Outcomes were analysed via a random effects model and reported as relative risk (RR) with 95% CI. The certainty of evidences was assessed by adapted GRADE. The reliabilities of meta-analyses were analysed by p value plots. RESULTS: Seventy studies met our inclusion criteria. (1) Short-term exposure to BC/EC was associated with 1.6% (95% CI 0.4% to 2.8%) increase in cardiovascular diseases per 1 µg/m3 in the elderly; (2) Long-term exposure to BC/EC was associated with 6.8% (95% CI 0.4% to 13.5%) increase in cardiovascular diseases and (3) The p value plot indicated that the association between BC/EC and respiratory diseases was consistent with randomness. CONCLUSIONS: Both short-term and long-term exposures to BC/EC were related with cardiovascular diseases. However, the impact of BC/EC on respiratory diseases did not present consistent evidence and further investigations are required. PROSPERO REGISTRATION NUMBER: CRD42020186244.


Subject(s)
Cardiovascular Diseases , Cardiovascular System , Respiration Disorders , Aged , Carbon/adverse effects , Cardiovascular Diseases/etiology , Humans , Particulate Matter/analysis , Reproducibility of Results , Respiration Disorders/etiology
12.
RSC Adv ; 11(28): 17352-17359, 2021 May 06.
Article in English | MEDLINE | ID: mdl-35479671

ABSTRACT

Developing high-performance electrocatalysts for urea oxidation reaction (UOR) can not only solve the problem of environmental pollution, but also solve the problem of the energy crisis by producing hydrogen for electrodes. The preparation of porous three-dimensional nanostructures as efficient electrocatalysts has become important work. Here, we developed a novel three-dimensional (3D) nanostructure of NiFe(OH) X nanoparticles/nickel foam with a high active area by a simple electroplating method and a subsequent treatment with ferric ion solution. This structure shows much greater UOR activity than the control sample (Ni/Ni foam) with the potential of 1.395 V (vs. RHE) (with an overpotential of 1.025 V) for driving the current density of 100 mA cm-2 in 1.0 M KOH electrolyte with 0.33 M urea. This work not only provides rapid and large-scale preparation of a three-dimensional nanostructure, but also gives a new way to design and obtain high-performance electrocatalysts.

13.
Opt Express ; 30(7): 10346-10353, 2022 Mar 28.
Article in English | MEDLINE | ID: mdl-35473004

ABSTRACT

Coherence and entanglement are both the fundamental properties which quantify the degree of nonclassicality possessed in a quantum state. Recently coherence and entanglement are considered as a dynamical resource where the nonclassicality is strongly related to the amount of the static resources which can be generated in a quantum process. In [Phys. Rev. Lett.125, 130401 (2020)10.1103/PhysRevLett.125.130401], for the first time, the authors study the interconvertability of these two kinds of dynamical resources. Here, we demonstrate this resource conversion in an all optical setup, and successfully observe the dynamical resource conversion. The experimental observation prove the ability of manipulating dynamical resource within current quantum photonic technologies.

14.
Analyst ; 147(10): 2106-2114, 2022 May 17.
Article in English | MEDLINE | ID: mdl-35470820

ABSTRACT

Conductivity detectors are widely used electrochemical sensors. It has long been a goal of researchers to improve detection performance. In this contribution, we propose a multi-input capacitively coupled contactless conductivity detector (MIC4D) with high sensitivity, and we carry out a detailed theoretical investigation of the detector. In order to overcome the problem of a rising baseline level as a result of sensitivity improvements when using the multi-input detection method, we innovatively combine MIC4D with differential detection to propose a further-improved detector (DFMIC4D). The detector is composed of two channels, one for the reference and the other for the analyte. The signal output from differential amplification can effectively reduce the high baseline level and detection interference. In KCl solution with a concentration range of 10-4 to 10-5 M, the response to the solution is a linear function of the logarithm of the concentration, and this detector has a high slope. The slope of DFMIC4D is 1.393, higher than a traditional single-input capacitively coupled contactless conductivity detector (C4D: 0.905) and a double-input capacitively coupled contactless conductivity detector (DIC4D: 1.314). For 10-3 M KCl solution, the response-to-baseline ratio is 1.776 for C4D, 1.779 for DIC4D, and 12.06 for DFMIC4D, with a ratio increase of nearly 6-fold shown by our new detector. At a S/N (signal-to-noise) ratio of 3, the limit of detection (LOD) of DFMIC4D is low, reaching 0.7 nM. In addition, DFMIC4D can be applied to the detection of low-conductivity solutions and total dissolved solids (TDS) analysis. Compared with a standard conductivity meter, our detector shows better detection performance.

15.
Front Cell Dev Biol ; 10: 857919, 2022.
Article in English | MEDLINE | ID: mdl-35445019

ABSTRACT

In recent years, fatty acid binding protein 5 (FABP5), also known as fatty acid transporter, has been widely researched with the help of modern genetic technology. Emerging evidence suggests its critical role in regulating lipid transport, homeostasis, and metabolism. Its involvement in the pathogenesis of various diseases such as metabolic syndrome, skin diseases, cancer, and neurological diseases is the key to understanding the true nature of the protein. This makes FABP5 be a promising component for numerous clinical applications. This review has summarized the most recent advances in the research of FABP5 in modulating cellular processes, providing an in-depth analysis of the protein's biological properties, biological functions, and mechanisms involved in various diseases. In addition, we have discussed the possibility of using FABP5 as a new diagnostic biomarker and therapeutic target for human diseases, shedding light on challenges facing future research.

16.
Front Cardiovasc Med ; 9: 851214, 2022.
Article in English | MEDLINE | ID: mdl-35433881

ABSTRACT

Background: This study aimed to investigate the impact of the COVID-19 pandemic on ST-segment elevation myocardial infarction (STEMI) care in China. Methods: We conducted a multicenter, retrospective cohort study in Hunan province (adjacent to the epidemic center), China. Consecutive patients presenting with STEMI within 12 h of symptom onset and receiving primary percutaneous coronary intervention, pharmaco-invasive strategy and only thrombolytic treatment, were enrolled from January 23, 2020 to April 8, 2020 (COVID-19 era group). The same data were also collected for the equivalent period of 2019 (pre-COVID-19 era group). Results: A total of 610 patients with STEMI (COVID-19 era group n = 286, pre-COVID-19 era group n = 324) were included. There was a decline in the number of STEMI admissions by 10.5% and STEMI-related PCI procedures by 12.7% in 2020 compared with the equivalent period of 2019. The key time intervals including time from symptom onset to first medical contact, symptom onset to door, door-to-balloon, symptom onset to balloon and symptom onset to thrombolysis showed no significant difference between these two groups. There were no significant differences for in-hospital death and major adverse cardiovascular events between these two groups. Conclusion: During the COVID-19 pandemic outbreak in China, we observed a decline in the number of STEMI admissions and STEMI-related PCI procedures. However, the key quality indicators of STEMI care were not significantly affected. Restructuring health services during the COVID-19 pandemic has not significantly adversely influenced the in-hospital outcomes.

17.
Chemosphere ; 300: 134608, 2022 Apr 14.
Article in English | MEDLINE | ID: mdl-35430204

ABSTRACT

China has implemented two national clean air actions in 2013-2017 and 2018-2020, respectively, with the aim of reducing primary emissions and hence improving air quality at a national level. It is important to examine the effectiveness of such emission reductions and assess the resulting changes in air quality. However, such evaluation is difficult as meteorological factors can amplify, or obscure the changes of air pollutants, in addition to the emission reduction. In this study, we applied the random forest machine learning technique to decouple meteorological influences from emissions changes, and examined the deweathered trends of air pollutants in 12 Chinese mega-cities during 2013-2020. The observed concentrations of all criteria pollutants except O3 showed significant declines from 2013 to 2020, with PM2.5 annual decline rates of 6-9% in most cities. In contrast, O3 concentrations increased with annual growth rates of 1-9%. Compared with the observed results, all the pollutants showed smoothed but similar variation in trend and annual rate-of-change after weather normalization. The response of O3 to NO2 concentrations indicated significant regional differences in photochemical regimes, and the differences between observed and deweathered results provided implications for volatile organic compound emission reductions in O3 pollution mitigation. We further evaluated the effectiveness of first and second clean air actions by removing the meteorological influence. We found that the meteorology can make negative or positive contribution in reducing pollutant concentrations from emission reduction, depending on type of pollutants, locations, and time period. Among the 12 mega-cities, only Beijing showed a positive meteorological contribution in amplifying reductions in main pollutants except O3 during both clean air action periods. Considering the large and variable impact of meteorological effects in changing air quality, we suggest that similar deweathered analysis is needed as a routine policy evaluation tool on a regional basis.

18.
Acc Chem Res ; 55(9): 1301-1312, 2022 05 03.
Article in English | MEDLINE | ID: mdl-35416643

ABSTRACT

Escalating energy demand, the depletion of fossil fuels, and abnormal climate change are recognized as the key challenges in the 21st century. The valorization of biomass and plastic, representing the most abundant natural and man-made polymers, respectively, as alternatives to fossil fuel is one of the promising solutions to creating a carbon-neutral, waste-free society. Catalysis is an essential tool for manipulating energy transformations via bond-breaking and bond-forming principles. To producing chemicals and fuels via biomass valorization and plastic upcycling, the cleavage of C-O and C-C bonds is the major catalytic route, given that the two are mainly constructed by various interunit C-O and C-C linkages. In this work, a consensus concerning the catalytic mechanism is reached: the activities for the cleavage of C-O and C-C bonds highly depend on the catalyst ability to activate the C-O and C-C bonds. Among the catalysts reported, NbOx-based catalysts show a unique, superstrong ability to activate C-O and C-C bonds. While research on biomass valorization over NbOx-based catalysts maintains its momentum, plastic upcycling driven by an efficient NbOx-based catalyst capable of activating C-O and C-C bonds is quickly catching up. Therefore, deepening the understanding of NbOx-based catalysts for the activation of C-O and C-C bonds is of importance to further drive biomass valorization and plastic upcycling, even in many other related areas. Herein, we present progress on the activation of C-O and C-C bonds in waste carbon resources, with an emphasis on our own work in using NbOx-based catalysts. First, we introduce NbOx-based catalysts for the activation of C-O and C-C bonds in biomass with a special focus on explaining how NbOx-based catalysts activate C-O and C-C bonds and why NbOx-based catalysts can activate C-O and C-C bonds so efficiently. Then, unified descriptors to embody the abilities to extract O from oxygenated compounds and an adsorbed benzene ring, namely "oxygen affinity" and "benzene ring affinity", were defined to standardize C-O and Carom-Caliph activation chemistry. Furthermore, we highlight the emerging opportunities of NbOx-based catalysts for plastic upcycling by learning the wisdom accumulated from the activation of C-O and C-C bonds in biomass. Finally, our own insights into future recommendations in this promising field are provided.


Subject(s)
Benzene , Carbon , Biomass , Catalysis , Humans , Plastics
19.
J Chromatogr A ; 1671: 463011, 2022 May 24.
Article in English | MEDLINE | ID: mdl-35398699

ABSTRACT

The micropores of the metal-organic frameworks (MOFs) endow with the advantages of size selectivity and large specific surface area, etc., but they limit their applications toward the control of diffusion and transport processes. Here, we report a strategy to prepare the hierarchical porous material, functional defect, which allows incorporation of L-Cysteine in the MOF by defect-loading. Silica microspheres were modified with these materials to form core-shell composites and used as mixed-mode stationary phases for chromatographic separations. Compared with the traditional MOFs-based stationary phase, it exhibited superior efficiency and selectivity for separation of various analytes and highlighted the role of defect of MOFs and function of L-Cysteine. In addition to the rapid separation of hydrophobic compounds, the stationary phase showed great potential in the separation of hydrophilic analytes, especially for separation of ten carbohydrates and eight sulfonamides. It showed excellent chromatographic reproducibility and stability, and the repeatability of preparation was investigated by relative standard deviations of retention time and/or column efficiency of objective compounds, which was among different batches less than 1.81%. This demonstration provided deep understanding of separation mechanism between MOF-based composites with functional defect and analytes, and stimulated the wide applications of such defective MOFs complexes in separations and analysis.


Subject(s)
Metal-Organic Frameworks , Chromatography, Reverse-Phase/methods , Cysteine , Hydrophobic and Hydrophilic Interactions , Metal-Organic Frameworks/chemistry , Reproducibility of Results
20.
Xenobiotica ; : 1-11, 2022 Apr 13.
Article in English | MEDLINE | ID: mdl-35377274

ABSTRACT

The objective of this study was to identify metabolites of PD110 by UHPLC-Q-Exactive Plus MS and determine its metabolic pathways in vivo.Mouse urine, faeces, and plasma samples were collected after an intraperitoneal administration of PD110 at a single dose of 30 mg·kg-1.The metabolites were detected and identified by UHPLC-Q-Exactive Plus MS and Compound DiscovererTM 2.0 software.In total, 44 metabolites (including 31 phase-I and 13 phase-II metabolites) were preliminarily identified according to the mass accuracy (<5 ppm) and comparison of their mass spectrometry profiles. Oxidation, glucuronide conjugation, and glucoside conjugation were the main metabolic pathways of PD110 in mice.This research first focussed on the biotransformation of PD110 in mice, and its metabolites may provide pivotal information for further pharmacological and clinical studies.

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