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1.
PLoS ONE [Electronic Resource] ; 18(1):e0280577, 2023.
Article in English | MEDLINE | ID: covidwho-2197161

ABSTRACT

Since the beginning of the SARS-CoV-2 pandemic, supply chain shortages have caused major disruptions in sourcing the materials needed for laboratory-based molecular assays. With increasing demand for molecular testing, these disruptions have limited testing capacity and hindered efforts to mitigate spread of the virus and new variants. Here we evaluate an economical and reliable protocol for the extraction and short-term ambient temperature storage of SARS-CoV-2 RNA. Additional objectives of the study were to evaluate RNA from this protocol for 1) detection of single nucleotide polymorphisms (SNPs) in the spike gene and 2) whole genome sequencing of SARS-CoV-2. The RNAES protocol was evaluated with residual nasopharyngeal (NP) samples collected from Emory Healthcare and Emory Student Health services. All RNAES extractions were performed in duplicate and once with a commercial extraction robot for comparison. Following extraction, eluates were immediately tested by rRT-PCR. SARS-CoV-2 RNA was successfully detected in 56/60 (93.3%) RNAES replicates, and Ct values corresponded with comparator results. Upon testing in spike SNP assays, three genotypes were identified, and all variant calls were consistent with those previously obtained after commercial extraction. Additionally, the SARS-RNAES protocol yield eluate pure enough for downstream whole genome sequencing, and results were consistent with SARS-CoV-2 whole genome sequencing of eluates matched for Ct value. With reproducible results across a range of virus concentrations, the SARS-RNAES protocol could help increase SARS-CoV-2 diagnostic testing and monitoring for emerging variants in resource-constrained communities.

2.
2022 IEEE International Conference on Engineering Veracruz, ICEV 2022 ; 2022.
Article in English | Scopus | ID: covidwho-2191852

ABSTRACT

As of the start of COVID-19 pandemic, the need to change the modality in which classes were being taught arose in the administrative computer systems undergraduate program of the faculty of accounting and administration of the Universidad Veracruzana, which brought within the problem to ensure that the learning process was equally effective under dual modality. The main goal of the presented research is to carry out a correlational analysis to determine if certain variables had a positive impact in the students learning process under the face-to-face and virtual modalities. An exploratory and descriptive research was carried under a quantitative data collection to test a hypothesis based on a statistical and inferential analysis. The research shows, it can be stated that the learning delivery model for the selected sample was better under the face-to-face modality. And that the use of technological platforms for online classes was a factor that positively contributed to online learning. © 2022 IEEE.

3.
Open Forum Infectious Diseases ; 9(Supplement 2):S762, 2022.
Article in English | EMBASE | ID: covidwho-2189941

ABSTRACT

Background. Healthcare workers are at high risk of Covid-19 (C19) infection and received priority for C19 vaccinations. Therefore, we conducted a serosurvey to determine anti-C19 antibodies and evidence of C19 infection in health care employees who did or did not have direct contact with patients. Methods. 49 participants provided finger stick blood samples collected onto filter papers and tested for antibodies to C19 using Bio-Plex Pro Human SARS-CoV-2 IgG reagents. Antibodies to C19 nucleocapsid (N), receptor-binding domain (RBD), spike 1 (S1), and spike 2 (S2) were measured. Samples were collected 8 to 11 months after C19 vaccines were made available. Results. All participants received two doses of Pfizer BioNTech or Moderna RNA-based C19 vaccines, and all showed serological evidence of antibodies to C19 RBD, S1, and S2. Antibodies to N, considered a marker of C19 infection, were detected in 16 individuals, of whom 10 reported having a PCR documented C19 infections. 6 individuals had evidence of C19 infection of which they were not aware. Antibody levels were notably higher following infection and for not infected participants following Pfizer-BioNTech vaccination. There was a 20% higher infection rate in participants with direct patient contact. Conclusion. This vaccinated population had significant rates of strong antibody responses to C19 infection and a notable rate of C19 infections, most notable in those providing direct patient care.

4.
Revista de Salud Publica ; 24(3), 2022.
Article in Spanish | Scopus | ID: covidwho-2204139

ABSTRACT

Objective The School Feeding Program (PAE, by its initials in Spanish) in Colombia provides a food supplement for children and adolescents enrolled in the public educational system;however, due to the COVID-19 pandemic, its delivery modality had to be modified. The purpose of this study was to describe the strategies of the CEDID Ciudad Bolívar School to implement the PAE during the COVID-19 pandemic. Methods Methodological design based on semi-structured interviews with teachers, students and parents of families belonging to this school. The information was analyzed according to a set of previously established categories. Results The participants agree that the transfer of parents due to loss of employment and not having technological devices influences school dropout. On the other hand, the school took advantage of the transitory modality of the PAE, delivering bonds redeemable by market;however, they affirm that not all were able to access it, due to ignorance of the process. It is highlighted by the participants that it is a food aid, it is economic, and that it manages to benefit the whole family, but the high cost of food, the shortage of supplies, and the difficulties when it comes to redeeming it are evident. Conclusions Despite the pandemic, the institution has made efforts to adapt the PAE to epidemiological circumstances, in order to face the risk of school dropout and guarantee the food supplement for students, being valued by them and their families. © 2022, Universidad Nacional de Colombia. All rights reserved.

5.
Innovar ; 33(87):93-108, 2022.
Article in English | Scopus | ID: covidwho-2204131

ABSTRACT

This study sought to understand the factors behind the intention towards buying organic products mentioned in the existing literature and provide conceptual and theoretical guidelines for future research on the subject. A systematic revision of original research papers included in Scopus and Web of Science databases during the period 2011-2021 was conducted, resulting in a sample of 162 articles. The results show that the attitude, values, and confidence of the consumer were the main factors in the intention to buy organic foods. Moreover, it was found that the theory of planned behavior was the most used theoretical framework in the analysis of organic foods purchasing. The changes brought by the covid-19 pandemic are manifested in consumers' values and their implications to the sale of this type of foods. We propose to consider consumers' values as variables that intertwine the rational and motivational approaches to consumption analysis. It is also proposed to understand the purchase intention underlying the stimulation of attitudes and the confidence of consumers due to their values. Finally, it is suggested to consider the analysis of the mediating effect of attitudes and trust in the relationship between values and purchase intention. © 2022, Universidad Nacional de Colombia. All rights reserved.

6.
Tecnologia En Marcha ; 35:74-83, 2022.
Article in Spanish | Web of Science | ID: covidwho-2121173

ABSTRACT

During the COVID-19 pandemic facing the world, it became necessary to search for low-cost strategies to guarantee people's health;while economic, educational, and social activities that were seriously affected are being reactivated. As research on the virus progresses, the relationship between the probability of transmission of COVID-19 and indoor air quality has been discovered, indicating that virus particles can accumulate easier in spaces where ventilation and air quality are deficient. This article describes the methodologies implemented from different disciplines for the design of a system, which is made up of a circuit, a casing, and a desktop application. The design of this system will allow the monitoring of air quality variables in the classrooms and laboratories of the Tecnologico de Costa Rica, by warning when spaces have poor air quality, and therefore the place must be ventilated.

7.
Educatio Siglo Xxi ; 40(3):133-160, 2022.
Article in English | Web of Science | ID: covidwho-2110729

ABSTRACT

Achieving student participation and invol-vement in the development of each sub-ject is a long-standing challenge in higher education. On the basis of this premise, this article focuses on studying the levels of student participation in virtual and fa-ce-to-face education environments in the third year of the degree in Modern Lan-guages and Translation at the University of Alcala. More specifically, our analysis aims to compare students' general impres-sions of their participation in both con-texts (on-site teaching and virtual teaching during the university closure due to the COVID-19 pandemic) and to probe their opinion on the participation tools used by the teaching staff with a focus on which of those tools they consider most and least useful in each context. To achieve these aims, a two-stage study combining quanti-tative and qualitative data was developed through two coalescent data collection procedures: an online questionnaire res-ponded by 47 out of 70 students and a subsequent virtual discussion group with five students. The analysis of the informa-tion collected consolidates participation as a problematic aspect in the teaching -learning process in both environments, although it also shows a year-on-year im-provement in virtual contexts. Similarly, the results show a widespread support of the instruments used by teachers to boost the levels of student participation in both online and face-to-face sessions.

8.
PLoS Global Public Health ; 2(8), 2022.
Article in English | CAB Abstracts | ID: covidwho-2039233

ABSTRACT

Comprehensive data on transmission mitigation behaviors and both SARS-CoV-2 infection and serostatus are needed from large, community-based cohorts to identify COVID-19 risk factors and the impact of public health measures. We conducted a longitudinal, population-based study in the East Bay Area of Northern California. From July 2020-March 2021, approximately 5,500 adults were recruited and followed over three data collection rounds to investigate the association between geographic and demographic characteristics and transmission mitigation behavior with SARS-CoV-2 prevalence. We estimated the populated-adjusted prevalence of antibodies from SARS-CoV-2 infection and COVID-19 vaccination, and self-reported COVID-19 test positivity. Population-adjusted SARS-CoV-2 seroprevalence was low, increasing from 1.03% (95% CI: 0.50-1.96) in Round 1 (July-September 2020), to 1.37% (95% CI: 0.75-2.39) in Round 2 (October-December 2020), to 2.18% (95% CI: 1.48-3.17) in Round 3 (February-March 2021). Population-adjusted seroprevalence of COVID-19 vaccination was 21.64% (95% CI: 19.20-24.34) in Round 3, with White individuals having 4.35% (95% CI: 0.35-8.32) higher COVID-19 vaccine seroprevalence than individuals identifying as African American or Black, American Indian or Alaskan Native, Asian, Hispanic, two or more races, or other. No evidence for an association between transmission mitigation behavior and seroprevalence was observed. Despite >99% of participants reporting wearing masks individuals identifying as African American or Black, American Indian or Alaskan Native, Asian, Hispanic, two or more races, or other, as well as those in lower-income households, and lower-educated individuals had the highest SARS-CoV-2 seroprevalence and lowest vaccination seroprevalence. Results demonstrate that more effective policies are needed to address these disparities and inequities.

9.
Revista Galega de Economia ; 31(1), 2022.
Article in Spanish | Scopus | ID: covidwho-2025770

ABSTRACT

Due to the significant contraction in economic activity and employment throughout the world caused by Covid-19, it is especially pertinent to continue advancing in the study of the effect that the implementation of socially responsible practices has on profitability. Thus, in this empirical research, carried out with a sample of 6,186 companies, it is intended to deepen the existing relationship between the effort made by the Spanish business fabric within the manufacturing sector in social responsibility policies and the results obtained in its financial indicators. For this, we focus on the relationships that the company maintains with its external stakeholders, the community, with human resources and policies related to quality management, and we analyze how they contribute to improving the company's economic profitability (ROA), using a dashboard of data. The results show that the implementation of collaboration policies to increase and improve relationships with suppliers, competitors and institutions has a significant positive effect on increasing ROA. However, collaboration with clients had a negative impact on ROA. In addition, the application of human resources practices aimed at improving relations with employees and the implementation of quality policies also have a positive and relevant impact on ROA © Universidade de Santiago de Compostela.

10.
Scandinavian Journal of Immunology ; 95(6), 2022.
Article in English | EMBASE | ID: covidwho-1968184

ABSTRACT

Patients with haematological malignancies are prioritized for COVID-19 vaccine due to their high risk for SARS-CoV-2 infection related disease severity and mortality. Immune defects associated with malignancy and their treatment could influence vaccine driven immune response in these cancer patients. Thus, to understand T cell immunity, its long-term persistence, and correlation with antibody response, we evaluated the BNT162b2 SARS-CoV-2 mRNA vaccine-specific immune response in chronic lymphocytic leukaemia (CLL) and myeloid dysplastic syndrome (MDS) patients. Longitudinal analysis of CD8+ T cells using DNA-barcoded peptide-MHC multimers covering the full SARS-CoV-2 spike-protein showed vaccine-specific T cell activation and persistence of memory T cells up to 6 months post-vaccination. Surprisingly, a higher frequency of vaccine-induced antigen-specific CD8+ T cell was observed in the patient group compared to the healthy donor group. Furthermore, and importantly, immunization with the second booster dose significantly increased the frequency of antigen-specific CD8+ T cells as well as the total number of T cell specificities. Altogether, 23 immunogenic epitopes were identified and a strong immunodominance was observed for the two of the spike-specific T cell epitopes restricted to HLA-A24 (NYNYLYRLF) and HLA-A2 (YLQPRTFLL) with a prevalence of 100% and 80% respectively. In summary, we mapped the vaccine-induced antigen-specific CD8+ T cells and showed a booster-specific activation and enrichment of memory T cells that could be crucial for long-term disease protection for this patient group.

11.
Proceedings of the European Conference on Agricultural Engineering, AgEng2021, Evora, Portugal ; 2021.
Article in English | CAB Abstracts | ID: covidwho-1918822

ABSTRACT

Involving students in collaborative projects and experiential learning helps develop transversal skills that, with passive learning, do not usually have specific training or reinforcement. Additionally, certain transversal skills such as the ability to communicate and coordinate in interdisciplinary work groups are essential for our graduates in their professional activity. The project consisted of a simulation of a real work environment in which a multidisciplinary team, made up of students from two different subjects of different degrees, must coordinate their efforts to propose a coherent solution to a problem. This project aims to reinforce the skills and competencies necessary both for the development of the rest of the subjects and for the subsequent exercise of the profession. These skills require reinforcement not only from the teacher but also from the classmates themselves, who would be competitors, students and the jury at the same time when assessing the results of the rest of the students. The problem to be solved focused on a proposal related to the supply of energy for a hypothetical agricultural industry, where agricultural engineering students carried out a design project for an electrical installation and the building students participated in the project by conducting an energy audit. Through collaborative work, it has been seen that students show a total willingness to work with students of other degrees and that the learning process improves since there is a greater interaction of skills and knowledge, which gives them an interdisciplinary vision of what it would perform a real job. Despite the difficult situation motivated by COVID pandemic, the project was successful and in total 25 students participated demonstrating that cooperative learning improves transversal skills.

12.
Virologie ; 26(2):173-174, 2022.
Article in English | EMBASE | ID: covidwho-1913029

ABSTRACT

The emergence of SARS-CoV-2 has triggered a pandemic with devastating consequences to the world. One of the proteins essential to the virus life cycle is nsp14, which is a bifunctional protein that encodes a 3'to 5' exoribonuclease activity in its N-terminus, and a methyl transferase activity in its C-terminus. Nsp14 in complex with the accessory protein nsp10 is involved in a proofreading mechanism that ensures the genetic stability of its massive viral genome, and is associated to the resistance against nucleotide analogs targeting the polymerase nsp12. Because of its key role, nsp14-nsp10 complex constitutes an attractive target for antiviral development. Here we present a fluorescence polarization (FP) assay development to measure the exoribonuclease activity and its inhibition in vitro. The FP method is sensitive, robust, amenable to miniaturization and offers confirmation by visualizing the degradation of the fluorescent RNA in acrylamide gels. We performed a screening of a focused library of 113 metal chelators at 20 and 5 μM compound concentration and IC50 measurement of 9 hits showing efficiency at micromolar level. We also tested the focused library in SARS-CoV-2 infected Vero cells and we confirmed 3 hits previously detected in the in vitro screening out of 6 promising inhibitors. In conclusion the FP method proposed is a reliable tool to discover inhibitors of the SARS-CoV-2 exoribonuclease activity and will help to find new antivirals to be used in combination with nucleoside analogs.

13.
International Journal of Safety and Security in Tourism/Hospitality ; 22(49), 2021.
Article in English | CAB Abstracts | ID: covidwho-1871997

ABSTRACT

The objective of this paper is to analyze the strategies and actions implemented by small and medium-sized companies in the tourism sector (SMEs) based on the social and economic crisis caused by COVID-19. The study focuses mainly on direct customer service on the coastline of the Metropolitan Area of Puerto Vallarta, including souvenir shops, accommodation, restaurants, and art galleries, among others. Our argument is that to keep operating, companies had to implement various changes, mainly the incorporation of digital tools in their management and communication processes with both suppliers and their customers. The research uses quantitative methods, both digital and traditional (face-to-face) instruments were used to data collection in order to overcome the obstacles imposed by the social distancing, and confinement required in the general population. The results show what were the activities implemented by the companies that contribute to innovation, as well as particularly two types of innovation, according to the Oslo Manual (2007) in relation to commercial and organizational innovations. We conclude that tourism is an activity of direct contact with the consumer and experience, which limits certain services to be digitalized, so that it must be considered the profile of tourists, the age group and their region of origin to explain the acceptance to digitalization of some practices related to the provision of tourist services.

15.
Med Intensiva (Engl Ed) ; 46(7): 420-421, 2022 07.
Article in English | MEDLINE | ID: covidwho-1804834
16.
Urvio-Revista Latinoamericana De Estudios De Seguridad ; - (31):62-76, 2021.
Article in Spanish | Web of Science | ID: covidwho-1573001

ABSTRACT

The SARS-CoV-2 pandemic has questioned the dominant international security strategies, and also highlighted the immense contradictions in our global way of life. From a human security perspective, this investigation analyses the pandemic as a risk and threat to social life on a global scale. The conceptual categories of human security and global risk are used to understand the inequalities, structural violence and vulnerabilities that accompany the health emergency and make it a total social crisis. Some of the main risk trends that the pandemic represents are quantitatively and qualitatively analyzed. It is proposed that the global pandemic is leading to the suppression of the loopholes of security and certainty provided by modernity, whose contradictions have led us to suffer the most important planetary catastrophe in recent history. Likewise, the pandemic has shown that the strategies anchored to traditional security are not the most appropriate to face the consequences that the current health crisis will have. Therefore, to respond to these risks and threats, it is essential to develop new perspectives on security.

17.
Scandinavian Journal of Immunology ; 94(6):1, 2021.
Article in English | Web of Science | ID: covidwho-1548340
18.
Research and Practice in Thrombosis and Haemostasis ; 5(SUPPL 2), 2021.
Article in English | EMBASE | ID: covidwho-1508982

ABSTRACT

Background : Patients affected by COVID-19 are at risk of various venous and arterial thrombotic events, as well as embolic risks, the risk can vary from 17% to 78% according to the different published series. therefore, thromboprophylaxis must be imperative. Aims : To assess the use of rivaroxaban as thromboprophylaxis in recovered COVID-19 patients who are at thrombotic risk Methods : Descriptive and analytical study in patients who presented pneumonia due to COVID-19 in April and May 2020, who received LMWH during their hospital stay and at discharge with rivaroxaban 10 mg/day for 2 months. D-dimer was measured at the beginning of the study and 1 month after discharge. Thrombotic or hemorrhagic episodes are controlled after 1 and 2 months of treatment (June -July 2020). Results : 50 patients, women 40% and men 60%, with a median age of 42.9 years. 64% had mild pneumonia and 36% had severe pneumonia, mean initial D-dimer 556.5 ng/ml, 56% had D-dimer ≥ 500 ng/ ml at the time of hospital admission. Baseline D-dimer values were significantly higher in severe pneumonia. In the follow-up at one and two months, we found that D-dimer values were significantly higher among patients with severe pneumonia and also, in this group of patients, the percentage of patients with D-dimer levels ≥ 500 ng/mL in the first month of follow-up, was significantly higher in the group of patients who were hospitalized for severe pneumonia. During the first month of follow-up, there was a thrombotic event and a hemorrhagic event in the group of patients with severe pneumonia;By the second month of follow-up, there was a hemorrhagic event in the group with mild pneumonia, but this difference in frequencies was not statistically significant. TABLE 1 Variables stratified by type of pneumonia Conclusions : The incidence of thrombotic and hemorrhagic events was low, so the thromboprophylaxis scheme used in patients with recovered pneumonia due to COVID-19 is recommended. Rivaroxaban is safe to use like thromboprophylaxis.

19.
Annals of Hepatology ; 24, 2021.
Article in English | EMBASE | ID: covidwho-1446405

ABSTRACT

Introduction: Some patients with SARSCov-2 infection develop severe disease (SARS);however, the factors associated with severity are not yet fully understood. Some reports indicate that liver injury may be a poor prognostic factor. Aim: To identify the biochemical factors related to the development of SARS with mechanical ventilation (MV) requirement in patients with SARSCov-2 and COVID-19. Methods Type of study: Observational. Cohort study. Procedure: Data from COVID-19 patients were collected at admission time to a tertiary care center. Differential factors were identified between seriously ill SARS+MV patients versus stable patients without MV. Transformation to the natural logarithm of significant variables was performed and multiple linear regression was applied, then a predictive model of severity called AAD (Age-AST-D dimer) was constructed. Results: 166 patients were included, 114(68.7%) men, mean age 50.6±13.3 years-old, 27(16.3%) developed SARS+MV. In the comparative analysis between those with SARS+MV versus stable patients without MV we found significant raises of ALT (225.4±341.2 vs. 41.3±41.1;P=0.003), AST 325.3±382.4 vs. 52.8±47.1;P=0.001), LDH (764.6±401.9 vs. 461.0±185.6;P=0.001), D dimer (7765±9109 vs. 1871±4146;P=0.003), age (58.6±12.7 vs. 49.1±12.8;P=0-001). The results of the regression are shown in the Table, where model 3 was the one that best explained the development of SARS+MV;with these variables was constructed the model called AAD, where: [AAD= 3.896 + ln(age)x-0.218 + ln(AST)x-0.185 + ln(DD)x0.070], where a value ≤ 2.75 had sensitivity=0.797 and 1-specificity= 0.391, AUROC=0.74 (95%CI: 0.62-0.86;P<0.0001), to predict the risk of developing SARS+MV (OR=5.8, 95%CI: 2.2-15.4;P=0.001). Conclusions: Elevation of AST (probable marker of liver damage) is an important predictor of progression to SARS, together with elevation of D-dimer and age early (at admission) and efficiently predict which patients will potentially require MV.

20.
Anesthesia and Analgesia ; 133(3 SUPPL 2):1603-1604, 2021.
Article in English | EMBASE | ID: covidwho-1445000

ABSTRACT

Introduction: Personal protective equipment (PPE) required for the care of patients with SARS-CoV-2 may impair communication between healthcare providers, increasing the potential for medical error. However, it is unclear how the type of PPE and characteristics of healthcare providers impact communication. Methods: 75 healthcare workers at Memorial Hospital West completed a 19 item survey to qualitatively assess the impact of gender, age, healthcare role (preop nursing, recovery nursing, OR nursing, surgeon, surgery assistant, anesthesia), native language (English, other), respirator type (N95, P100) and eye protection type (face shield, goggles) on employee perception of communication. The Bamford- Kowal-Bench(BKB) sentence list, a benchmarked tool for evaluating comprehension of verbal communication, was utilized to quantitatively analyze communication between representative healthcare workers wearing varying PPE was assessed. Variables in the quantitative analysis included distance between workers (3 versus 6 feet), phone versus in person communication, and ambient noise level (60 dB, 90dB). An Iasus GP3-R throat microphone was evaluated as an intervention to improve communication. Results: In the qualitative survey, the age of the speaker / listener (41-60) and speaker respirator (P100) impacted communication more than other factors (p <0.05). All participants agreed that higher background noise and phone conversations represented additional risks. In the quantitative survey, comprehension scores were lower for speakers wearing a P100 respirator, regardless of eye protection. Noise level reduced comprehension to a greater extent on phone. Distance between speaker and listener did not impact comprehension. Native speakers with P100 were more easily understood than non-native speakers with P100. The use of Iasus GP3-R throat microphone improved comprehension except in the case of non-native speakers wearing a P100 respirator. Conclusions: Communication between workers is impacted by PPE and may contribute to medical error. Comprehension is affected by respirator type, native language of speaker, and age of listener / speaker.

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