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1.
Bioengineering ; 9(8):359, 2022.
Article in English | ProQuest Central | ID: covidwho-2023124

ABSTRACT

Bronchiectasis is defined as a permanent dilation of the bronchi that can cause pulmonary ventilation dysfunction. CT examination is an important means of diagnosing bronchiectasis. It can also be used in severity scoring. Current studies on bronchiectasis have focused on high-resolution CT (HRCT), ignoring the more common low-dose CT (LDCT). Methodologically, existing studies have not adopted an authoritative standard to classify the severity of bronchiectasis. In effect, the accuracy of detection and classification needs to be improved for practical application. In this paper, the ACER image enhancement method, RDU-Net lung lobe segmentation method and HDC Mask R-CNN model were proposed to detect and classify bronchiectasis. Moreover, a Python-based system was developed: after inputing an LDCT image of a patient’s lung, it can automatically perform a series of processing, then call on the trained deep learning model for detection and classification, and automatically obtain the patient’s bronchiectasis final score according to the Reiff and BRICS scoring criteria. In this paper, the mapping relationship between original lung CT image data and bronchiectasis scoring system was established. The accuracy of the method proposed in this paper was 91.4%;the IOU, sensitivity and specificity were 88.8%, 88.6% and 85.4%, respectively;and the recognition speed of one picture was about 1 s. Compared to a human doctor, the system can process large amounts of data simultaneously, quickly and efficiently, with the same judgment accuracy as a human doctor. Doctors only need to judge the uncertain cases, which significantly reduces the burden of doctors and provides a useful reference for doctors to diagnose the disease.

2.
Ocean & Coastal Management ; 229:106338, 2022.
Article in English | ScienceDirect | ID: covidwho-2004391

ABSTRACT

A container shipping network connects coastal countries with each other and facilitates most of the world merchandise trade. Reliable maritime connectivity ensures the availability of commodities and economic growth. The global spread of COVID-19 has led to port failures and service cancellations, resulting in decreased connectivity level of container ports. To mitigate the impact of the pandemic, a graph theory approach is proposed to strength the container shipping network connectivity by considering topology and the possibility of opening new shipping links between ports. It is designed to maximize network connectivity with limited addable routes. The network connectivity is measured by algebraic connectivity, and the possibility of opening new shipping links is estimated by an extended gravity model. A heuristic algorithm based on Fiedler vector is introduced to obtain the optimal solutions. The performance of the proposed model and algorithm are verified by testing on a real-world container shipping network based on the Alphaliner database. Experimental results illustrate that the presented model is efficient and effective for strengthening the connectivity. Policy makers can refer to the suggested optimal shipping links to facilitate better shipping network connectivity in the context of the COVID-19 pandemic.

3.
Journal of International Students ; 12:68-87, 2022.
Article in English | ProQuest Central | ID: covidwho-2002868

ABSTRACT

It is well established that international education can profoundly influence a student, including identity and agency formation and the acquisition of knowledge and culture. This study applies the concept of self-formation to reconceptualize the international student experience. It captures the development, changes, and operation of identity and agency during self-formation. Utilizing collective autoethnography, the authentic experiences of three international students studying in Australia during the Covid-19 pandemic were collected. These stories illustrate their transformation from international students to student researchers. Through thematic analysis, three phases have been identified in this study, which are self-exploration, self-positioning, and self-determination and shedding light on the role of agency and hybrid identity. A further scholarly investigation is advocated to enrich the discussion of self-formation and add a nuanced investigation into the variability of individual experiences.

4.
International Journal of Molecular Sciences ; 23(16):9342, 2022.
Article in English | MDPI | ID: covidwho-1997645

ABSTRACT

R2R3-MYB transcription factors participate in multiple critical biological processes, particularly as relates to the regulation of secondary metabolites. The dried root of Scutellaria baicalensis Georgi is a traditional Chinese medicine and possesses various bioactive attributes including anti-inflammation, anti-HIV, and anti-COVID-19 properties due to its flavonoids. In the current study, a total of 95 R2R3-MYB genes were identified in S. baicalensis and classified into 34 subgroups, as supported by similar exon–intron structures and conserved motifs. Among them, 93 R2R3-SbMYBs were mapped onto nine chromosomes. Collinear analysis revealed that segmental duplications were primarily responsible for driving the evolution and expansion of the R2R3-SbMYB gene family. Synteny analyses showed that the ortholog numbers of the R2R3-MYB genes between S. baicalensis and other dicotyledons had a higher proportion compared to that which is found from the monocotyledons. RNA-seq data indicated that the expression patterns of R2R3-SbMYBs in different tissues were different. Quantitative reverse transcriptase-PCR (qRT-PCR) analysis showed that 36 R2R3-SbMYBs from different subgroups exhibited specific expression profiles under various conditions, including hormone stimuli treatments (methyl jasmonate and abscisic acid) and abiotic stresses (drought and cold shock treatments). Further investigation revealed that SbMYB18/32/46/60/70/74 localized in the nucleus, and SbMYB18/32/60/70 possessed transcriptional activation activity, implying their potential roles in the regulatory mechanisms of various biological processes. This study provides a comprehensive understanding of the R2R3-SbMYBs gene family and lays the foundation for further investigation of their biological function.

5.
Preprint in English | medRxiv | ID: ppmedrxiv-22279589

ABSTRACT

BACKGROUNDThe rising breakthrough infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, especially Omicron and its sub-lineages, have raised an urgent need to develop broad-spectrum vaccines against coronavirus disease 2019 (COVID-19). We have developed a mosaic-type recombinant vaccine candidate, named NVSI-06-09, having immune potentials against a broad range of SARS-CoV-2 variants. METHODSAn ongoing randomized, double-blind, controlled phase 2 trial was conducted to evaluate the safety and immunogenicity of NVSI-06-09 as a booster dose in subjects aged 18 years and older from the United Arab Emirates (UAE), who had completed two or three doses of BBIBP-CorV vaccinations at least 6 months prior to the enrollment. The participants were randomly assigned with 1:1 to receive a booster dose of NVSI-06-09 or BBIBP-CorV. The primary outcomes were immunogenicity and safety against SARS-CoV-2 Omicron variant, and the exploratory outcome was cross-immunogenicity against other circulating strains. RESULTSA total of 516 participants received booster vaccination. Interim results showed a similar safety profile between NVSI-06-09 and BBIBP-CorV booster groups, with low incidence of adverse reactions of grade 1 or 2. For immunogenicity, by day 14 after the booster vaccination, the fold rises in neutralizing antibody geometric mean titers (GMTs) from baseline level elicited by NVSI-06-09 were remarkably higher than those by BBIBP-CorV against the prototype strain (19.67 vs 4.47-fold), Omicron BA.1.1 (42.35 vs 3.78-fold), BA.2 (25.09 vs 2.91-fold), BA.4 (22.42 vs 2.69-fold), and BA.5 variants (27.06 vs 4.73-fold). Similarly, the neutralizing GMTs boosted by NVSI-06-09 against Beta and Delta variants were also 6.60-fold and 7.17-fold higher than those boosted by BBIBP-CorV. CONCLUSIONSA booster dose of NVSI-06-09 was well-tolerated and elicited broad-spectrum neutralizing responses against SARS-CoV-2 prototype strain and immune-evasive variants, including Omicron and its sub-lineages. The immunogenicity of NVSI-06-09 as a booster vaccine was superior to that of BBIBP-CorV. (Funded by LIBP and BIBP of Sinopharm; ClinicalTrials.gov number, NCT05293548).

6.
Remote Sensing ; 14(11):2622, 2022.
Article in English | ProQuest Central | ID: covidwho-1892936

ABSTRACT

The Greenhouse Gases Observing Satellite (GOSAT) can help to ascertain the global distribution of carbon dioxide (CO2) and methane (CH4), and how the sources and sinks of these gases vary by season, year, and location. However, the data provided by the GOSAT level 2 and 3 products have certain limitations due to their lack of spatial and temporal information;even with the application of the kriging geostatistical method on the level 2 products, the processing algorithms still need further upgrades. In this study, we apply an empirical orthogonal function (EOF)-based method on the GOSAT L3 products (137 images, from January 2010 to May 2021) to estimate the column average of carbon dioxide and methane (XCO2–XCH4) within the entire Earth. The reconstructed results are validated against the Total Carbon Column Observing Network (i.e., TCCON), with 31 in situ stations, and GOSAT L4B column-averaged data, using 107 layers. The results show an excellent agreement with the TCCON data and exhibit an R-squared coefficient of 0.95 regarding the CO2 measurements and 0.86 regarding the CH4 measurements. Therefore, this methodology can be incorporated into the processing steps used to map global greenhouse gases.

8.
EuropePMC; 2022.
Preprint in English | EuropePMC | ID: ppcovidwho-337236

ABSTRACT

With the persistence of the COVID-19 pandemic caused primarily by constant viral mutations, rapid identification of different lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by large-scale screening at the point-of-care could be key to monitoring and assessing viral evolutions. Herein, we developed a Fluorescence Enhanced Microarray for Multiplex Analysis of Nucleic acids (FEMMAN) for detecting 8 SARS-CoV-2 variants simultaneously in ~ 3 hours without the need of RNA extraction, opening the possibility of point-of-care testing of multiple SARS-CoV-2 variants while reducing the cost significantly to ~ $ 7 per sample from ~ $100 by Next-Generation Sequencing (NGS). Combined with isothermal amplification, the multiplexed RNA assay achieved single-copy detection sensitivity and single nucleotide variant (SNV) distinction owing to the nanotechnology based plasmonic gold (pGOLD) near-infrared fluorescence enhancing platform. Probing 10 targets of three mutational hotspots in S gene, we differentiated 8 viral lineages (Wild type, Alpha, Beta, Gamma, Delta, Lambda, Mu, and Omicron) of SARS-CoV-2, validated using nasopharyngeal swabs obtained from 127 individuals, achieving a 100% sensitivity and 100% specificity in SARS-CoV-2 detection, and a 91.1% concordance with NGS in variant identification. The scalable, multiplexed FEMMAN assay could shift the paradigm of COVID-19 diagnostic and surveillance from positive/negative assessments to simultaneous lineage identification in large-scale screening, greatly facilitating the global monitoring of SARS-CoV-2 variants.

9.
EuropePMC; 2022.
Preprint in English | EuropePMC | ID: ppcovidwho-335387

ABSTRACT

Background: Due to the similar symptoms between COVID-19 and malaria, it’s difficult to identify the differences between them. We firstly report a patient of recurrent COVID-19 and Plasmodium falciparum co-infection. Case presentation She was confirmed with COVID-19 95 days ago. The laboratory tests showed she had high inflammation results. The SARS-CoV-2 IgG was positive, but the IgM was negative. A rapid serum detection for malaria was finished and found with Plasmodium falciparum . The patient received levofloxacin for anti-infection,and Dihydroartemisinin piperaquine was to clear the parasite. A combined of Lopinavir (LPV), Arbidol Hydrochloride Granules (AHG), and Recombinant Human interferon a2b atomization inhalation (IAI) was used to treat COVID-19. Conclusion: Under the pandemic time of COVID-19, it is best to do a combined test for the COVID-19 and other tropical diseases, which could be better for us to early diagnosis and early treatment. To a recurrent COVID-19 patient, we believe that the level of SARS-CoV-2 IgG in convalescent stage is 4 times or more than that in acute stage is a good sign for discharge.

10.
Frontiers in immunology ; 13, 2022.
Article in English | EuropePMC | ID: covidwho-1787237

ABSTRACT

Coronavirus disease 2019 (COVID-19) has evolved into an established global pandemic. Metabolomic studies in COVID-19 patients is worth exploring for further available screening methods. In our study, we recruited a study cohort of 350 subjects comprising 248 COVID-19 patients (161 non-severe cases, 60 asymptomatic cases, and 27 severe cases) and 102 healthy controls (HCs), and herein present data with respect to their demographic features, urinary metabolome, immunological indices, and follow-up health status. We found that COVID-19 resulted in alterations of 39 urinary, mainly microbial, metabolites. Using random forest analysis, a simplified marker panel including three microbial metabolites (oxoglutaric acid, indoxyl, and phenylacetamide) was constructed (AUC=0.963, 95% CI, 0.930-0.983), which exhibited higher diagnostic performance than immune feature-based panels between COVID-19 and HC groups (P<0.0001). Meanwhile, we observed that urine metabolic markers enabled discriminating asymptomatic patients (ASY) from HCs (AUC = 0.981, 95% CI, 0.946-0.996), and predicting the incidence of high-risk sequalae in COVID-19 individuals (AUC=0.931, 95% CI, 0.877-0.966). Co-expression network analysis showed that 13 urinary microbial metabolites (e.g., oxoglutaric acid) were significantly correlated with alterations of CD4+, CD3+, and CD8+ T-cells, as well as IFN-γ, IL-2 and IL-4 levels, suggesting close interactions between microbial metabolites and host immune dysregulation in COVID-19. Taken together, our findings indicate that urinary metabolites may have promising potential for screening of COVID-19 in different application scenarios, and provide a new entry point to understand the microbial metabolites and related immune dysfunction in COVID-19.

11.
Chinese Preventive Medicine ; 22(4):246-249, 2021.
Article in Chinese | CAB Abstracts | ID: covidwho-1761323

ABSTRACT

Objective: To understand the epidemiological characteristics of a cluster of COVID-19 caused by an imported case in late December 2020, and to provide reference for the prevention and control of COVID-19.

12.
Industrial Management & Data Systems ; 122(3):702-728, 2022.
Article in English | ProQuest Central | ID: covidwho-1752275

ABSTRACT

Purpose>In the pandemic time, many local governments in China issue mobile coupons to citizens in order to boost economy via stimulating consumption. This study examines value drivers for consumers to use government issued mobile coupons (GIMCs), which differ from traditional ones issued by retailers, during the pandemic time in mainland China.Design/methodology/approach>Content analysis of 148 posts and 388 comments from Sina Weibo were performed to identify value-related antecedents of GIMCs' usage by consumers. Then, drawing on extant literature and based on the variables extracted from content analysis, a conceptual model was proposed addressing the determinants of value perceptions which motivate consumers to acquire and redeem GIMCs. A questionnaire survey was conducted among 510 citizens who used government issued m-coupons to verify hypotheses.Findings>The research findings show that functional value (driven by economic benefits, convenience and relevance), emotional value (driven by trustworthiness, competition and altruism) and social value (driven by social norms and social recognition) positively shape consumer's attitude toward GIMCs and their intention to use GIMCs. Also, it is found that functional value and attitude link is enhanced by consumer's utilitarian shopping motivation and weakened by hedonic shopping motivation. Hedonic shopping motivation enhances the relationship between emotional value and attitude.Originality/value>This research contributes to the theory development of coupon promotional tool by focusing on m-coupons issued by government (instead of retailers) under the pandemic background. Besides, it provides managerial insights for local authorities to design effective GIMCs policies.

13.
Preprint in English | bioRxiv | ID: ppbiorxiv-486173

ABSTRACT

Large-scale populations in the world have been vaccinated with COVID-19 vaccines, however, breakthrough infections of SARS-CoV-2 are still growing rapidly due to the emergence of immune-evasive variants, especially Omicron. It is urgent to develop effective broad-spectrum vaccines to better control the pandemic of these variants. Here, we present a mosaic-type trimeric form of spike receptor-binding domain (mos-tri-RBD) as a broad-spectrum vaccine candidate, which carries the key mutations from Omicron and other circulating variants. Tests in rats showed that the designed mos-tri-RBD, whether used alone or as a booster shot, elicited potent cross-neutralizing antibodies against not only Omicron but also other immune-evasive variants. Neutralizing antibody titers induced by mos-tri-RBD were substantially higher than those elicited by homo-tri-RBD (containing homologous RBDs from prototype strain) or the inactivated vaccine BBIBP-CorV. Our study indicates that mos-tri-RBD is highly immunogenic, which may serve as a broad-spectrum vaccine candidate in combating SARS-CoV-2 variants including Omicron.

14.
Preprint in English | medRxiv | ID: ppmedrxiv-22272062

ABSTRACT

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with immune escape ability raises the urgent need for developing cross-neutralizing vaccines against the virus. NVSI-06-08 is a potential broad-spectrum recombinant COVID-19 vaccine that integrates the antigens from multiple SARS-CoV-2 strains into a single immunogen. Here, we evaluated the safety and immunogenicity of NVSI-06-08 as a heterologous booster dose in adults previously vaccinated with the inactivated vaccine BBIBP-CorV in a randomized, double-blind, controlled, phase 2 trial conducted in the United Arab Emirates (NCT05069129). Three groups of healthy adults over 18 years of age (600 participants per group) who had administered two doses of BBIBP-CorV 4-6-month, 7-9-month and >9-month earlier, respectively, were vaccinated with either a homologous booster of BBIBP-CorV or a heterologous booster of NVSI-06-08. The primary outcome was immunogenicity and safety of booster vaccinations. The exploratory outcome was cross-reactive immunogenicity against multiple SARS-CoV-2 variants of concerns (VOCs). The incidence of adverse reactions was low in both booster vaccinations, and the overall safety profile of heterologous boost was quite similar to that of homologous boost. Heterologous NVSI-06-08 booster was immunogenically superior to homologous booster of BBIBP-CorV. Both Neutralizing and IgG antibodies elicited by NVSI-06-08 booster were significantly higher than by the booster of BBIBP-CorV against not only SARS-CoV-2 prototype strain but also multiple VOCs. Especially, the neutralizing activity induced by NVSI-06-08 booster against the immune-evasive Beta variant was no less than that against the prototype strain, and a considerable level of neutralizing antibodies against Omicron (GMT: 367.67; 95%CI, 295.50-457.47) was induced by heterologous booster, which was substantially higher than that boosted by BBIBP-CorV (GMT: 45.03; 95%CI, 36.37-55.74). Our findings showed that NVSI-06-08 was safe and immunogenic as a booster dose following two doses of BBIBP-CorV, which was immunogenically superior to homologous boost with another dose of BBIBP-CorV. Our study also indicated that the design of hybrid antigen may provide an effective strategy for broad-spectrum vaccine developments.

15.
Pacific-Basin Finance Journal ; : 101736, 2022.
Article in English | ScienceDirect | ID: covidwho-1699320

ABSTRACT

To fill the gaps between managerial distraction and disclosure quality of management earnings forecasts (MEFs), we examine the effects of managerial selective attention resulting from the COVID-19 pandemic. Using this pandemic in 2020 as an adverse shock potentially causing managerial distraction, results based on a difference-in-differences estimation suggest that managerial distraction had a negative effect on MEFs and affected forecast quality by increasing work burden and perceptual narrowing among managers, while cash reserves demonstrated a preventive function and alleviated such adverse effects. Our findings are robust, as supported by tests that address potential measurement errors.

16.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-325187

ABSTRACT

Background: 2019-nCoV has posed a significant threat to human health since the epidemic began. So far, there is no definite safe and effective drug treatment. Proper management can help prevent mild patients from becoming severe. We report a couple with different symptoms after simultaneously infected by the same source of 2019-nCoV and analyze the changes in the diagnosis and treatment process. Case presentation We report a couple with different symptoms after simultaneously infected by the same source of 2019-nCoV in Shandong, China,describe their clinical course,characteristics and detection results in detail, and analyze the changes in the diagnosis and treatment process. The wife was mainly affected by respiratory system and had ever experienced high fever for several days. The husband's condition is lighter than his wife,mainly manifested as digestive tract symptoms,but it took longer than his wife for the nucleic acid of nasopharynx swab for 2019-nCoV to turn negative. Conclusions: In the course of the disease both of the couple had hypokalemia which was easy to be corrected. CRP and ESR were consistent with the changes of the disease during the 2019-nCoV infection, which were good indicators to reflect the severity and development of the disease. Two months after discharge, both of them showed negative IgM antibody and positive IgG antibody.

17.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-325010

ABSTRACT

Background: Since December 2019, COVID-19 has rapidly swept the world. It is particularly important to understand the dynamic changes of the whole disease course of non-severe patients from the onset to the follow-up after discharge. Methods: : On February 1, 2020, 18 cases of non-severe COVID-19 appeared in a hospital in Beijing. All patients were SARS-CoV-2 RNA positive by RT-PCR for pharyngeal swabs. We recorded the clinical information and viral dynamics of these patients from the onset of the disease to 2 months after discharge. According to the severity of lung consolidation, 18 patients were divided into two groups (mild pulmonary consolidation group [imaging score ≤10];severe pulmonary consolidation group [imaging score >10]). Results: : Eighteen patients (median age 43) were included, including 14 females. Fever (11/18) and cough (8/18) were the most common symptoms. The duration of SARS-CoV-2 RNA positive in mild pulmonary consolidation group was significantly longer than severe pulmonary consolidation group (the median time was 30 days and 13 days, respectively, P= 0.0031). Two months after discharge, almost all patients were followed up for IgM antibody disappearance and IgG antibody production. Conclusion: In non-severe COVID-19 patients, the positive duration of the SARS-CoV-2 RNA in patients with mild pulmonary consolidation was longer than the severe pulmonary consolidation. However, it is necessary for a large sample to verify our conclusions.

18.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-324280

ABSTRACT

Background: Until now, information on the clinical characteristics of severe and critical patients with COVID-19 is extremely limited.The aim of the present study was to analyse the clinical features of these patients and influencing factors of clinical outcome, and explore treatment effects of prone position on COVID-19 patients with severe ARDS. Methods A retrospective analysis was performed on 55 COVID-19 patients in the ICU of Zhongnan Hospital of Wuhan University from January 6 to February 15, 2020 in Wuhan, China. Case data from each patient were collected and related clinical outcomes on day 14 of ICU admission were recorded. The follow-up deadline was February 29, 2020. Results Of the 55 patients included, 35 were male (63.6%), with an average age of 63.0 (SD 15.2) years, and 80.0% were patients over 50 years old. The first three symptoms were fever (36 cases, 65.5%), fatigue (13 cases, 23.6%), and cough (11 cases, 20.0%). The rate of invasive mechanical ventilation was 52.7% (29 cases);on the 14th day of ICU admission, 31 patients(56.4%) were improved, and 19 (34.5%) were worsened. On the 14th day after entering the ICU, a comparative analysis showed that peripheral blood CD4, CD8, and NK cell counts in deteriorated patients were significantly lower than those in improved patients (P<0.05). Meanwhile, concentrations of IL-10, IL-4, IL-6 and TNF-α in deteriorated patients were higher than those in improved patients (P <0.05). Among a total of 27 prone position sessions, the oxygenation index (PaO2 / FiO2 ) of 9 prone position sessions(33.3%) improved, and the PaCO2 in arterial blood gas analysis of 5 sessions(18.5%) improved. Conclusion The majority of patients with severe and critical COVID-19 in the ICU were over 50 years old and male. 52.7% need invasive mechanical ventilation. On the 14th day of admission, 56.4% of the patients improved, 34.5% of the patients deteriorated. The rate of deaths during hospitalization was 21.8%. The worsening of COVID-19 patients might be related to excessive inflammatory and immune responses. In addition, prone ventilation may improve oxygenation in some COVID-19 patients with severe ARDS, but a significant mortality benefit with proning was uncertain.

19.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-324224

ABSTRACT

Background: SARS-Coronavirus-2 (SARS-CoV-2), the pathogen of coronavirus disease 2019 (COVID-19), not only infects the respiratory tract, but also other organs. About a third of the inpatients of COVID-19 have neurological symptoms and in vitro experiments revealed that SARS-CoV-2 could infect human neural progenitor cells and brain organoids. However, the traditional test often reports negative owing to the low number of virus in the cerebrospinal fluid. To date, timely diagnosis of central nervous system infection of SARS-CoV-2 remains a challenge. Case presentation: On day 14 of COVID-19, seizures , maxillofacial convulsions, intractable hiccups and significant increase in intracranial pressure developed in a 56-year-old man. The RT-PCR of SARS-CoV-2 was negative. SARS-CoV-2 nucleic acid were detected in cerebrospinal fluid (CSF) by ultrahigh depth sequencing. The patient was successfully treated after 14 days of mechanical ventilation and treatment of pneumonia and neurological dysfunction. Conclusions: : This case suggests SARS-CoV-2 can invade the central nervous system and relevant examinations with CSF including ultrahigh depth sequencing of SARS-CoV-2 are needed among COVID-19 patients with neurological dysfunction.

20.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-323646

ABSTRACT

Background: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is a new emerging public health crisis to the world. However, data are still limited on the clinical features and laboratory findings in COVID-19 patients. Methods: : Medical records including demographics characteristics, clinical features, laboratory findings and radiological materials of 66 hospitalized COVID-19 patients were collected between Jan 23 and Mar 9, 2020. Symptoms/signs with potential association with the disease severity were analyzed. Results: : Of 66 hospitalized COVID-19 patients, the male-to-female ratio was 44:22. There were eight cases potentially exposed to one single patient. The most common initial symptoms of SARS-CoV-2 infection were fever (77.3%) and cough (74.2%). Compared to those with non-severe infection, the severe patients were more likely to be older (62.6 ± 15.1 vs 46.9 ± 13.3 years. P = 0.001) and with more infected lobes. As the results shown, higher initial (on admission) and peak (during hospitalization) counts of lymphocyte were inversely associated with the severe SARS-CoV-2 infection (both OR: 0.01 every 1´10 9 /L decrease). However, the elevated initial neutrophil counts (OR: 1.63 every 1´10 9 /L increase), initial and peak levels of LDH (OR: 1.02 and 1.01 every 1 U/L increase), peak levels of CRP (OR: 1.03 every 1 mg/L increase), AST (OR: 1.06 every 1 U/L increase) and ALT (OR: 1.02 every 1 U/L increase) were significantly associated with COVID-19 severity. Conclusion: Our present study indicated that fever and cough were the most common initial symptoms of SARS-CoV-2 infection, and the virus could be efficiently spread by person-to-person transmission. In addition, lymphocyte and neutrophil counts, and serum levels of AST, ALT, CRP and LDH should be useful for the evaluation on COVID-19 severity.

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