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Critical Care Medicine ; 51(1):230-230, 2023.
Article in English | Web of Science | ID: covidwho-2309821
Interiors-Design Architecture Culture ; : 1-21, 2023.
Article in English | Web of Science | ID: covidwho-2309491
Interiors-Design Architecture Culture ; 2022.
Article in English | Web of Science | ID: covidwho-2242570
Biosensors and Bioelectronics ; 221, 2023.
Article in English | Scopus | ID: covidwho-2240295
ACS Sustainable Chemistry and Engineering ; 2022.
Article in English | Scopus | ID: covidwho-2235729


Here, we developed a copper sulfate (CuSO4)-initiated diphenylamine (DPA)-based colorimetric strategy coupled with loop-mediated isothermal amplification (LAMP) for rapid detection of two critical contagious pathogens, SARS-CoV-2 and Enterococcus faecium. To detect the DNA, acid hydrolysis of LAMP amplicons was executed, enabling the development of a blue color. In the LAMP amplicons, the bond between the purines and deoxyribose is extremely labile. It can be broken using 70% sulfuric acid followed by phosphate group elimination, which generates a highly active keto aldehyde group. CuSO4 plays an imperative role inducing DPA to rapidly react with the keto aldehyde group, producing an intense blue color within 5 min. Moreover, low quantities such as 103 copies μL-1 of SARS-CoV-2 RNA and 102 CFU mL-1 of E. faecium were successfully detected, revealing the advantages of the introduced method. To confirm practical applicability, multiplex detection of pathogens was performed using a foldable microdevice comprising reaction and detection zones. Various reactions such as DNA extraction, LAMP, and acid hydrolysis occurred in the reaction zone. Then, colorimetric reagents (DPA, CuSO4, and ethylene glycol) contained in the detection zone were mixed with the keto aldehyde group by simply folding the microdevice, which was heated at 65 °C for 5 min for colorimetric detection. An intense blue color was developed where the target DNA was present. These results indicate that the method proposed in this study is highly suitable for point-of-care applications, especially in resource-limited settings for the rapid detection of harmful pathogens. © 2023 American Chemical Society

Critical Care Medicine ; 51(1 Supplement):230, 2023.
Article in English | EMBASE | ID: covidwho-2190563
Asia Pacific Journal of Tourism Research ; 27(9):967-985, 2022.
Article in English | Web of Science | ID: covidwho-2134276
Iranian Journal of Public Health ; 51(11):2472-2483, 2022.
Article in English | Web of Science | ID: covidwho-2125594
Success in Social Marketing: 100 Case Studies From Around the Globe ; : 1-392, 2022.
Article in English | Scopus | ID: covidwho-2100167
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; : 1515-1516, 2021.
Article in English | Scopus | ID: covidwho-2012143
Jurnal Komunikasi: Malaysian Journal of Communication ; 38(2):251-264, 2022.
Article in English | Scopus | ID: covidwho-1955179
Korea Observer ; 53(1):105-133, 2022.
Article in English | Web of Science | ID: covidwho-1856657