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1.
International Journal of Environmental Research and Public Health ; 20(2):1416, 2023.
Article in English | MDPI | ID: covidwho-2200108

ABSTRACT

The outbreak and spreading of COVID-19 since early 2020 have dramatically impacted public health and the travel environment. However, most of the studies are devoted to travel behavior from the macro perspective. Meanwhile, few researchers pay attention to intercity travel behavior. Thus, this study explores the changes in the travel behavior of intercity high-speed railway travelers during the COVID-19 pandemic from the perspective of the individual. Using the smartphone data, this study first extracts the trip chains by proposing a novel method including three steps. The trip chain can describe the whole process of traveling, including individual characteristics, travel time, travel distance, travel mode, etc. Then, a Multinomial Logit model is applied to analyze the trip chains which verified the validity by using studentized residual error. The study finds that intercity travel behavior has changed in gender, age, travel mode choice, and travel purpose by comparing the trip chains between May 2019 and May 2021 in the Beijing-Tianjin-Hebei urban agglomeration. The method proposed in this study can be used to assess the impact of any long-term emergency on individual travel behavior. The findings proposed in this study are expected to guide public health management and travel environment improvement under the situation of normalized COVID-19 prevention and safety control.

2.
Frontiers in Behavioral Neuroscience ; 2023.
Article in English | ProQuest Central | ID: covidwho-2199038

ABSTRACT

Background: Many countries now have relied on the various types of vaccines for the public to control COVID-19 pandemic. The adverse reactions (ARs) after vaccination may affect the vaccination coverage and confidence. However, whether the sleep quality was associated with the ARs after vaccination remains unclear. Methods: We designed a longitudinal paired study within a hospital setting. We collected data about the side effects within 7 days after two-dose schedule vaccination among healthcare workers (HCWs). All HCWs were asked to complete sleep survey indexed by the Pittsburgh Sleep Quality Index (PSQI) before vaccination and after one-month following up. We then explored the relationship between the sleep quality before or after vaccination and the occurrence of ARs. Results: A total of 345 HCWs were recruited to receive COVID-19vaccination. The sleep quality became poorer after vaccination. All local and systemic reactions were mild or moderate in severity (32.46%) and no serious adverse event was reported. Binary logistic regression showed participants with poor sleep quality (PSQI > 5) than good sleep quality (PSQI ≤5) before two dose vaccination respectively exhibited 1.515 and 1.107 times risk of ARs after each vaccination (both p < 0.001). Conclusions: There is an apparently complex bidirectional relationship between sleep quality and COVID-19 vaccination adverse effects. Poor sleep quality significantly increases the risk of mild ARs after vaccination, while vaccination may cause a temporary decline in sleep quality.

3.
Journal of Knowledge Management ; 27(1):47-58, 2023.
Article in English | ProQuest Central | ID: covidwho-2191536

ABSTRACT

Purpose>This paper aims to unveil the importance of knowledge management on a firm's strategic emergency response during the great negative shock from global public health threats. Through analyzing how representative firms in China's new economy industries dealt with the COVID-19 pandemic before, during and after the crisis, the significant problems confronted by these firms are pointed out, and the important role knowledge management capabilities played in solving these problems is proven.Design/methodology/approach>The open data of listed companies regarding the important role knowledge management played in firms' strategic emergency response during the COVID-19 pandemic are qualitatively analyzed. Based on theoretical sampling, this paper selects representative samples of enterprises and analyzes the positive response measures they took after being hit by this public health event to gain qualitative insight into the importance of knowledge management capabilities in strategic emergency response.Findings>Three aspects of the important role of knowledge management capabilities in a firm's strategic emergency response during the COVID-19 pandemic are introduced: before the crisis, firms should strengthen the acquisition, sharing and integration of knowledge so that they can intensify their monitoring for uncertain risks;during the crisis, firms should boost the transmission, transformation and diffusion of knowledge to improve emergency cooperation;and after the crisis, companies should reinforce knowledge evaluation, creation and application to enhance "immunity” in similar emergencies.Research limitations/implications>This paper has important implications for bolstering strategic emergency management practice and knowledge management capability among firms. Future research must focus on the following two aspects for further investigation: the dynamic relationship between firm knowledge management capability and strategic emergency response ability;and the collaboration system between firm knowledge management and strategic emergency response behaviors.Originality/value>This paper discusses the important role knowledge management capabilities play in firms' strategic emergency responses based on insights gained from the significant changes that the COVID-19 pandemic caused to representative Chinese new economy firms. By analyzing the three stages of before, during and after the emergency, this paper proposes the exact efforts that new economy companies should make in improving knowledge management capability.

4.
Journal of Southern Agriculture ; 53(8):2077-2087, 2022.
Article in Chinese | CAB Abstracts | ID: covidwho-2201259

ABSTRACT

Object: To explore genetic evolution relationship of variant porcine epidemic diarrhea virus(PEDV)and antigenic differential sites among variant strain subtypes,so as to lay a foundation for the development of novel vaccines and diagnostic kits. Method: Three PEDV-positive porcine intestinal samples were inoculated on to confluent Vero cells to isolate PEDV. Virus identification was performed by indirect fluorescence assay(IFA), Western blotting,RT-PCR and whole genome sequencing and electron microscopic observation;virus titer was determined by TCID50and the in vitvo proliferation dynamin curve of the virus was drawn. The genome of the isolated strain was divided into 33 segments for RT-PCR amplification, and the SeqMan of Lasergene was used to splice sequences. Then the genetic evolution analysis was performed with MEGA 7.0, and the antigenicity analysis was performed with Jameson-Wolf algorithm in Protean. Result: Typical cytopathic effect appeared in one PEDV-positive porcine intestinal sample in Vero cells when it was blindly passaged to the 6thgeneration and the sample was designated as CH-HK-2021. IFA and Western blotting results showed that the strain CH-HK-2021 could react with PEDV N monoclonal antibody and expected reads were obtained through RT-PCR amplification, which demonstrated this virus was PEDV. Diameter of strain CH-HK-2021 was 80-120 nm and the surface of the virus particles were in spike-like shape, indicating it was coronavirus. The strain could be stably propagated in Vero cells, and it has been passaged to 100thgeneration. After 24 h of infecting the Vero cells, virus titer of strain CH-HK-2021 reached the highest,105.6TCID50/mL. The size whole genome of strain CH-HK-2021 not including poly(A)tail was 28034 bp, with a similarity of 96.0%-98.9% with nucleotide sequence of the PEDV reference strain and a similarity of 93.1%-99.0% with S-base nucleotide sequence of the reference strain. The strain had the highest similarity with nucleotide sequence of variant strain CH/JX/01(KX058031)and the lowest similarity with nucleotide sequence of classical strain AVCT12(LC053455). Strain CH-HK-2021 was a subtype of G2a and it is spreading in China. Strain G2a and variant strain G2b had 42 nucleotide differential sites in S gene and 6 antigenic differential sites;and main differential sites located in subunit S2.

5.
Virology ; 579:29-37, 2023.
Article in English | ScienceDirect | ID: covidwho-2165943

ABSTRACT

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration and high mortality in neonatal piglets. The nucleocapsid (N) protein of PEDV is a highly conserved protein with strong immunogenicity and palys an important role in PEDV diagnosis. However, epitopes on the PEDV N protein have not yet been well characterized. Here, 32 monoclonal antibodies (mAbs) against the PEDV N protein were produced and identified. Six new epitopes were first identified by using a high-throughput epitope mapping method named AbMap. Sequence analysis revealed that among the six epitopes five epitopes were highly conserved among different PEDV strains. We also confirmed that the mAbs derived from the six epitopes of PEDV N protein, have no cross-reactivity with transmissible gastro enteritis virus or porcine delta coronavirus. These mAbs and their defined epitopes will help to understand the N protein structure and immunological characteristics, and to develop a rapid, accurate PEDV diagnosis method.

6.
Journal of Clinical Medicine ; 11(24):7517, 2022.
Article in English | MDPI | ID: covidwho-2163479

ABSTRACT

Background: During the COVID-19 pandemic, elective surgery has to undergo longer wait times, including nephrectomy for T1 renal cell carcinoma (RCC). This study aimed to investigate the time-to-surgery (TTS) of Chinese T1 RCC patients and its influencing factors, and to illustrate the impact of TTS on the prognosis of T1 RCC. Methods: We retrospectively enrolled 762 Chinese patients with pathological T1 RCC that underwent nephrectomy. To discover the impact of TTS on survival outcomes, we explored the possible delay intervals by week using the Kaplan-Meier method and Log-rank test. Cox proportional hazard models with inverse probability-treatment weighting (IPTW) were used to assess the association between TTS and disease-free survival (DFS) and overall survival (OS). Results: The median TTS of T1 RCC patients was 15 days. The Charlson comorbidity index, the Preoperative Aspects and Dimensions Used for an Anatomical (PADUA) score, and the maximal tumor diameter on presentation were independent influencing factors for TTS. The cut-off point of TTS was selected as 5 weeks according to the Log-rank analysis. For T1a RCC, patients with TTS > 5 weeks had similar DFS (HR = 2.39;95% CI, 0.82-6.94;p = 0.109) and OS (HR = 1.28;95% CI, 0.23-7.16;p = 0.779) compared to patients with TTS ≤5 weeks. For T1b RCC, patients with TTS > 5 weeks had shorter DFS (HR = 2.90;95% CI = 1.46-5.75;p = 0.002) and OS (HR = 2.49, 95% CI = 1.09-5.70;p = 0.030) than patients with TTS ≤5 weeks. Conclusions: Prolonged TTS had no impact on the prognosis of T1a RCC while surgery delayed for over 5 weeks may lead to worse survival in T1b RCC.

7.
Front Microbiol ; 13: 1008420, 2022.
Article in English | MEDLINE | ID: covidwho-2142114

ABSTRACT

Vaccination is one of the best ways to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) epidemic. Among the various SARS-CoV-2 vaccines approved for use, the BBIBP-CorV inactivated vaccine has been widely used in 93 countries. In order to understand deeply the protective mechanism of inactivated vaccine, which retains all antigenic components of live virus, the analysis of humoral responses triggered by multiple proteins is necessary. In this research, antibody responses were generated with 6 selected recombinant proteins and 68 overlapping peptides that completely covered SARS-CoV-2 nucleocapsid (N) protein in 254 healthy volunteers vaccinated with BBIBP-CorV. As a result, antibody responses to the receptor binding domain (RBD), N, and non-structural protein 8 (NSP8) were induced following immunization by BBIBP-CorV. The antibody responses detected in donors after the 2nd dose vaccination can be maintained for about 6 months. Moreover, specific antibody levels can be restored after the boosting vaccination measured by ELISA. Furthermore, the level of SARS-CoV-2 specific IgG response is independent of age and gender. Moreover, N391-408 was identified as a dominant peptide after vaccination of BBIBP-CorV through peptide screening. Understanding the overview of humoral reactivity of the vaccine will contribute to further research on the protective mechanism of the SARS-CoV-2 inactivated vaccine and provide potential biomarkers for the related application of inactivated vaccine.

8.
Applied Economics ; : 1-16, 2022.
Article in English | Web of Science | ID: covidwho-2121887

ABSTRACT

This paper investigates the spillover effect of lagged US daily returns on stock return predictability across 17 developed markets from January 1(st), 1972 through August 31(st), 2022. Using daily returns series, we find that lagged US returns is a superior predictor for future returns in international markets while including the lagged domestic returns and considering US negative or extreme returns. The predictive power of lagged US daily returns, nonetheless, substantially weakens during the recent COVID period. Our results imply that the degrees of stock return predictability and spillovers across markets are driven by the evolutionary market conditions, the channels of information transmission, and information leadership.

9.
Finance Research Letters ; 51:103506, 2023.
Article in English | ScienceDirect | ID: covidwho-2120210

ABSTRACT

This paper mainly investigates whether the climate policy uncertainty index (CPU) can predict the volatility of Chinese stock market volatility considering different sectors. Out-of-sample results show that climate policy uncertainty index can have a greater effect on the utility sector. We also investigate the effects of CPU based on longer horizons, different volatility levels and the COVID-19 pandemic. This paper tries to provide new evidence based on sector stock indices.

10.
Adv Sci (Weinh) ; : e2202689, 2022 Nov 15.
Article in English | MEDLINE | ID: covidwho-2115731

ABSTRACT

Infectious virus diseases, particularly coronavirus disease 2019, have posed a severe threat to public health, whereas the developed therapeutic and prophylactic strategies are seriously challenged by viral evolution and mutation. Therefore, broad-spectrum inhibitors of viruses are highly demanded. Herein, an unprecedented antiviral strategy is reported, targeting the viral glycan shields with hypervalent mannose-binding nanoparticles. The nanoparticles exhibit a unique double-punch mechanism, being capable of not only blocking the virus-receptor interaction but also inducing viral aggregation, thereby allowing for inhibiting the virus entry and facilitating the phagocytosis of viruses. The nanoparticles exhibit potent and broad-spectrum antiviral efficacy to multiple pseudoviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its major variants (D614G, N501Y, N439K, Δ69-70, Delta, and Omicron; lentiviruses expressing only the spike proteins), as well as other vital viruses (human immunodeficiency virus 1 and Lassa virus), with apparent EC50 values around the 10-9  m level. Significantly, the broad-spectrum inhibition of authentic viruses of both wild-type SARS-CoV-2 and Delta variants is confirmed. Therefore, this hypervalent glycan-shield targeting strategy opens new access to broad-spectrum viral inhibition.

11.
Front Psychiatry ; 13: 915225, 2022.
Article in English | MEDLINE | ID: covidwho-2109861

ABSTRACT

Objectives: Women with ovarian cancer (OC) have experienced unprecedented challenges since the novel coronavirus disease-2019 (COVID-19) outbreak in China. We aim to evaluate the experience of psychological status, physical symptoms and quality of life (QoL) and investigate the impact of COVID-19 pandemic on OC patients receiving olaparib. Methods: The survey was conducted online from April 22 to May 12 in 2020. Demographic and clinical questions were listed to collect general information. The degree of insomnia, depression, anxiety, stress symptoms and QoL were assessed by the Chinese versions of the Insomnia Severity Index, the Patient Health Questionnaire-9, the Generalized Anxiety Disorder-7, the Impact of Event Scale-Revised, and the General Functional Assessment of Cancer Therapy, respectively. Multivariate logistic regression analysis was conducted to analyze the risk factors for mental distress and QoL. Results: A total of 56 respondents coming from 15 various provinces in China participated in the survey. The prevalence of insomnia, depressive, anxiety, stress symptoms and reduced QoL were 37.5, 51.8, 37.5, 30.4, and 51.8%, respectively. Unfavorable disease status, shorter period of olaparib administration, adverse events of olaparib and delay in cancer care were correlated with mental health problems. Reduced QoL was also significantly associated with psychological distress. Conclusions: This study emphasized that mental health problems and reduced QoL should gain more attention in women with OC who are receiving oral olaparib at home. Appropriate psychological healthcare strategies are necessary for OC patients during the COVID-19 pandemic.

12.
Int Immunopharmacol ; 112: 109283, 2022 Nov.
Article in English | MEDLINE | ID: covidwho-2105145

ABSTRACT

BACKGROUND: Coronavirus disease 2019 (COVID-19) continues to be a major global public health challenge, with the emergence of variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Current vaccines or monoclonal antibodies may not well be protect against infection with new SARS-CoV-2 variants. Unlike antibody-based treatment, T cell-based therapies such as TCR-T cells can target epitopes that are highly conserved across different SARS-CoV-2 variants. Reportedly, T cell-based immunity alone can restrict SARS-CoV-2 replication. METHODS: In this study, we identified two TCRs targeting the RNA-dependent RNA polymerase (RdRp) protein in CD8 + T cells. Functional evaluation by transducing these TCRs into CD8 + or CD4 + T cells confirmed their specificity. RESULTS: Combinations of inflammatory and anti-inflammatory cytokines secreted by CD8 + and CD4 + T cells can help control COVID-19 in patients. Moreover, the targeted epitope is highly conserved in all emerged SARS-CoV-2 variants, including the Omicron. It is also conserved in the seven coronaviruses that infect humans and more broadly in the subfamily Coronavirinae. CONCLUSIONS: The pan-genera coverage of mutant epitopes from the Coronavirinae subfamily by the two TCRs highlights the unique strengths of TCR-T cell therapies in controlling the ongoing pandemic and in preparing for the next coronavirus outbreak.


Subject(s)
COVID-19 , SARS-CoV-2 , Humans , SARS-CoV-2/genetics , COVID-19/therapy , Epitopes , Receptors, Antigen, T-Cell/genetics , Antibodies, Monoclonal/therapeutic use , RNA-Dependent RNA Polymerase , Cytokines , Epitopes, T-Lymphocyte/genetics
13.
Front Public Health ; 10: 1015718, 2022.
Article in English | MEDLINE | ID: covidwho-2099277

ABSTRACT

Climate change and population aging are two of the most important global health challenges in this century. A 2020 study by the Environmental Protection Agency showed that average people, particularly older adults, spent 90% of their time at home. This is even more evident during the coronavirus disease 2019 (COVID-19) pandemic. Home-based care models have become a new trend. The health and comfort of the living environment profoundly impacts the wellbeing of older adults. Therefore, research on the physical environment of the family wards has become an inevitable part of promoting the health of older adults; however, current research is still lacking. Based on the study and analysis of continuous monitoring data related to elements of the physical environment (thermal comfort, acoustic quality, lighting quality, and indoor air quality) of family wards, this paper explores the living behaviors of the participants in this environmental research (open or closed windows, air conditioning, artificial lighting, and television) on the indoor physical environment. (1) While referring to the requirements of international standards for an indoor aging-friendly physical environment, we also discuss and analyze the physical environment parameter values according to Chinese standards. (2) People's life behaviors have different degrees of influence on the elements of indoor physical environments. For example, opening doors and windows can alleviate the adverse effects of indoor environmental quality on the human body better than simply turning on the air conditioner. (3) Owing to the decline in physical function, older adults need special care. Studying the status quo of physical environmental elements and proposing suitable environmental improvement measures for aging are of great significance. (4) This research aims to address global warming and severe aging and to contribute to sustainable environmental development.


Subject(s)
Air Pollution, Indoor , COVID-19 , Humans , Aged , COVID-19/epidemiology , Environment
14.
Journal of Safety Research ; 2022.
Article in English | ScienceDirect | ID: covidwho-2095709

ABSTRACT

Introduction: Autonomous vehicles (AVs) are considered a promising solution to improve seniors’ safety and mobility. However, to transition to fully automated transportation, especially among seniors, it is vital to assess their perception and attitude toward AVs. This paper investigates seniors’ perceptions and attitudes to a wide range of AV options from the perspective of pedestrians and users in general, as well as during and after the COVID-19 pandemic. Underlying this objective is to examine older pedestrians’ safety perceptions and behaviors at crosswalks in the presence of AVs. Method: A national survey collected data from a sample of 1,000 senior Americans. Using Principal Component Analysis (PCA) and Cluster Analysis, three clusters of seniors were identified with different demographic characteristics, perceptions, and attitudes toward AVs. Results: PCA findings revealed that “risky pedestrian crossing behavior,” “cautious pedestrian crossing behavior in the presence of AVs,” “positive perception and attitude toward shared AVs,” and “demographic characteristics” were the main components explaining most of the variation within the data, respectively. The PCA factor scores were used in the cluster analysis, which resulted in the identification of three distinctive groups of seniors. Cluster one included individuals with lower demographic scores and a negative perception and attitude toward AVs from the perspective of users and pedestrians. Clusters two and three included individuals with higher demographic scores. Cluster two included individuals with a positive perception toward shared AVs from the user perspective, but a negative attitude toward pedestrian-AV interaction. Cluster three included those with a negative perception toward shared AVs but a somewhat positive attitude toward pedestrian-AV interaction. Practical Applications: The findings of this study provide valuable insights to transportation authorities, AV manufacturers, and researchers regarding older American’s perception and attitude toward AVs as well as their willingness to pay and use Advanced Vehicle Technologies.

15.
J Voice ; 2022 Oct 17.
Article in English | MEDLINE | ID: covidwho-2069428

ABSTRACT

OBJECTIVES: Protective facial coverings (PFCs) such as surgical masks attenuate speech transmission and affect speech intelligibility, which is reported in languages such as English and German. The present study intended to verify the detrimental impacts on production of tonal languages such as Cantonese, by examining realization of speech correlates in Cantonese under PFCs including facial masks and shields. METHODS: We recorded scripted speech in Hong Kong Cantonese produced by three adult speakers who wore various PFCs, including surgical masks, KF94 masks, and face shields (with and without surgical masks). Spectral and temporal parameters were measured, including mean intensity, speaking rate, long-term amplitude spectrum, formant frequencies of vowels, and duration and fundamental frequency (F0) of tone-bearing parts. RESULTS: Significant changes were observed in all acoustic correlates of Cantonese speech under PFCs. Sound pressure levels were attenuated more intensely at ranges of higher frequencies in speech through face masks, whereas sound transmission was affected more at ranges of lower frequencies in speech under face shields. Vowel spaces derived from formant frequencies shrank under all PFCs, with the vowel /aa/ demonstrating largest changes in the first two formants. All tone-bearing parts were shortened and showed increments of F0 means in speech through PFCs. The decrease of tone duration was statistically significant in High-level and Low-level tones, while the increment of F0 means was significant in High-level tone only. CONCLUSIONS: General filtering effect of PFCs is observed in Cantonese speech data, confirming language-universal patterns in acoustic attenuation by PFCs. The various coverings lower overall intensity levels of speech and degrade speech signal in higher frequency regions. Modification patterns specific to Hong Kong Cantonese are also identified. Vowel space area is reduced and found associated with increased speaking rates. Tones are produced with higher F0s under PFCs, which may be attributed to vocal tension caused by tightened vocal tract during speaking through facial coverings.

16.
Transbound Emerg Dis ; 69(5): e2443-e2455, 2022 Sep.
Article in English | MEDLINE | ID: covidwho-2053020

ABSTRACT

The porcine deltacoronavirus (PDCoV) is a newly discovered pig enteric coronavirus that can infect cells from various species. In Haiti, PDCoV infections in children with acute undifferentiated febrile fever were recently reported. Considering the great potential of inter-species transmission of PDCoV, we performed a comprehensive analysis of codon usage patterns and host adaptation profiles of 54 representative PDCoV strains with the spike (S) gene. Phylogenetic analysis of the PDCoV S gene indicates that the PDCoV strains can be divided into five genogroups. We found a certain codon usage bias existed in the S gene, in which the synonymous codons are often ended with U or A. Heat map analysis revealed that all the PDCoV strains shared a similar codon usage trend. The PDCoV S gene with a dN/dS ratio lower than 1 reveals a negative selection on the PDCoV S gene. Neutrality analysis showed that natural selection is the dominant force in shaping the codon usage bias of the PDCoV S gene. Unexpectedly, host adaptation analysis reveals a higher adaptation level of PDCoV to Homo sapiens and Gallus gallus than to Sus scrofa. Compared to the USA lineage, the PDCoV strains in the Early China lineage and Thailand lineage were less adapted to their hosts, which indicates that the evolutionary process plays an important role in the adaptation ability of PDCoV. These findings of this study add to our understanding of PDCoV's evolution, adaptability, and inter-species transmission.


Subject(s)
Coronavirus Infections , Swine Diseases , Animals , Codon/genetics , Codon Usage , Coronavirus Infections/epidemiology , Coronavirus Infections/veterinary , Deltacoronavirus , Genome, Viral/genetics , Phylogeny , Swine , Swine Diseases/epidemiology
17.
Frontiers in psychiatry ; 13, 2022.
Article in English | EuropePMC | ID: covidwho-2046113

ABSTRACT

Objectives Women with ovarian cancer (OC) have experienced unprecedented challenges since the novel coronavirus disease-2019 (COVID-19) outbreak in China. We aim to evaluate the experience of psychological status, physical symptoms and quality of life (QoL) and investigate the impact of COVID-19 pandemic on OC patients receiving olaparib. Methods The survey was conducted online from April 22 to May 12 in 2020. Demographic and clinical questions were listed to collect general information. The degree of insomnia, depression, anxiety, stress symptoms and QoL were assessed by the Chinese versions of the Insomnia Severity Index, the Patient Health Questionnaire-9, the Generalized Anxiety Disorder-7, the Impact of Event Scale-Revised, and the General Functional Assessment of Cancer Therapy, respectively. Multivariate logistic regression analysis was conducted to analyze the risk factors for mental distress and QoL. Results A total of 56 respondents coming from 15 various provinces in China participated in the survey. The prevalence of insomnia, depressive, anxiety, stress symptoms and reduced QoL were 37.5, 51.8, 37.5, 30.4, and 51.8%, respectively. Unfavorable disease status, shorter period of olaparib administration, adverse events of olaparib and delay in cancer care were correlated with mental health problems. Reduced QoL was also significantly associated with psychological distress. Conclusions This study emphasized that mental health problems and reduced QoL should gain more attention in women with OC who are receiving oral olaparib at home. Appropriate psychological healthcare strategies are necessary for OC patients during the COVID-19 pandemic.

18.
International immunopharmacology ; 2022.
Article in English | EuropePMC | ID: covidwho-2046068

ABSTRACT

Background Coronavirus disease 2019 (COVID-19) continues to be a major global public health challenge, with the emergence of variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Current vaccines or monoclonal antibodies may not well be protect against infection with new SARS-CoV-2 variants. Unlike antibody-based treatment, T cell-based therapies such as TCR-T cells can target epitopes that are highly conserved across different SARS-CoV-2 variants. Reportedly, T cell-based immunity alone can restrict SARS-CoV-2 replication. Methods In this study, we identified two TCRs targeting the RNA-dependent RNA polymerase (RdRp) protein in CD8+ T cells. Functional evaluation by transducing these TCRs into CD8+ or CD4+ T cells confirmed their specificity. Results Combinations of inflammatory and anti-inflammatory cytokines secreted by CD8+ and CD4+ T cells can help control COVID-19 in patients. Moreover, the targeted epitope is highly conserved in all emerged SARS-CoV-2 variants, including the Omicron. It is also conserved in the seven coronaviruses that infect humans and more broadly in the subfamily Coronavirinae. Conclusions The pan-genera coverage of mutant epitopes from the Coronavirinae subfamily by the two TCRs highlights the unique strengths of TCR-T cell therapies in controlling the ongoing pandemic and in preparing for the next coronavirus outbreak.

19.
Front Immunol ; 13: 916411, 2022.
Article in English | MEDLINE | ID: covidwho-2022705

ABSTRACT

Coronavirus disease 2019 (COVID-19) has been raging all around the world since the beginning of 2020, and leads to acute respiratory distress syndrome (ARDS) with strong cytokine storm which contributes to widespread tissue damage and even death in severe patients. Over-activated immune response becomes one of the characteristics of severe COVID-19 patients. Regulatory T cells (Treg) play an essential role in maintaining the immune homeostasis, which restrain excessive inflammation response. So FOXP3+ Tregs might participate in the suppression of inflammation caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Besides suppressive function, tissue resident Tregs are also responsible for tissue repair. In this review, we mainly summarize the latest research focusing on the change of FOXP3+ Tregs in the COVID-19 patients, discuss the relationship between disease severity and number change of Tregs and speculate the potential role of FOXP3+ Tregs during SARS-CoV-2 infection. Furthermore, we introduce some potential Treg-based therapies to improve patients' outcomes, which include small molecular drugs, antibody drugs, CAR-Treg and cytokine treatment. We hope to reduce tissue damage of severe COVID-19 patients and offer better prognosis through Treg-based therapy.


Subject(s)
COVID-19 , T-Lymphocytes, Regulatory , COVID-19/immunology , Cytokine Release Syndrome , Forkhead Transcription Factors , Humans , Inflammation , SARS-CoV-2 , T-Lymphocytes, Regulatory/immunology
20.
Front Bioeng Biotechnol ; 10: 947895, 2022.
Article in English | MEDLINE | ID: covidwho-2022646

ABSTRACT

Digital PCR is the most advanced PCR technology. However, due to the high price of the digital PCR analysis instrument, this powerful nucleic acid detection technology is still difficult to be popularized in the general biochemistry laboratory. Moreover, one of the biggest disadvantages of commercial digital PCR systems is the poor versatility of reagents: each instrument can only be used for a few customized kits. Herein, we built a low-cost digital PCR system. The system only relies on low-cost traditional flat-panel PCR equipment to provide temperature conditions for commercial dPCR chips, and the self-made fluorescence detection system is designed and optically optimized to meet a wide range of reagent requirements. More importantly, our system not only has a low cost (<8000 US dollars) but also has a much higher universality for nucleic acid detection reagents than the traditional commercial digital PCR system. In this study, several samples were tested. The genes used in the experiment were plasmids containing UPE-1a fragment, TP53 reference DNA, hepatitis B virus DNA, leukemia sample, SARS-COV-2 DNA, and SARS-COV-2 RNA. Under the condition that DNA can be amplified normally, the function of the dPCR system can be realized with simpler and low-price equipment. Some DNA cannot be detected by using the commercial dPCR system because of the special formula when it is configured as the reaction solution, but these DNA fluorescence signals can be clearly detected by our system, and the concentration can be calculated. Our system is more applicable than the commercial dPCR system to form a new dPCR system that is smaller and more widely applicable than commercially available machinery.

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