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2.
Chinese Journal of Pharmaceutical Biotechnology ; 29(1):1-7, 2022.
Article in Chinese | EMBASE | ID: covidwho-1791590

ABSTRACT

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) can cause respiratory symptoms such as fever, cough and dyspnea after infection, and Coronavirus Disease 2019 (COVID-19), severe acute respiratory syndrome, and even death can occur in severe cases.SARS-CoV-2 infection has no specific treatment drugs, mainly rely on vaccination to block its transmission.In various structural proteins of SARS-CoV-2, Spike protein (S) and Nucleocapsid protein (N) are the main antigenic proteins, which are also important candidate proteins for developing SARS-CoV-2 vaccine and antibody detection reagents.To express chimeric protein containing multiple epitopes of SARS-CoV-2 by prokaryotic expression system, and to verify the immunogenicity of the chimeric protein, antigenic epitopes of SARS-CoV-2 structural proteins were analyzed and screened by molecular biology software, the selected antigenic epitopes were connected in tandem, and expressed high efficiently in E.coli as a chimeric protein by genetic engineering technology.The soluble chimeric protein of high purity was obtained after purification and renaturation.Mice were immunized with the purified chimeric protein together with MF59 adjuvant, aluminum adjuvant or no adjuvant at different doses respectively.Humoral immunity and cellular immunity induced by the chimeric protein were evaluated by detecting the antibody titer of antiserum and the level of related cytokine.The expressed chimeric protein was in the form of inclusion body and exists in the sediment, soluble chimeric protein was obtained after renaturation.The specific antibodies with high titer were produced in the immunized mice, and strong cellular immunity was induced also.Higher concentration of chimeric protein had better elicited immune effect than the lower concentration of chimeric protein.The immune effect induced by the chimeric protein with MF59 adjuvant was no different from that induced with aluminum adjuvant.This study provides novel ideas for the design and renaturation of SARS-CoV-2 chimeric protein, and the chimeric protein is expected to be used for the development of SARS-CoV-2 recombinant protein vaccine and diagnosis reagent.

3.
Zhonghua Wai Ke Za Zhi ; 60(5): 436-440, 2022 May 01.
Article in Chinese | MEDLINE | ID: covidwho-1771266

ABSTRACT

Telemedicine, which integrates medicine, communication, engineering, information and other disciplines, is a hot emerging cross field in recent years. With the development of telecommunication technology and surgical robot, telesurgery is regarded as the "crown pearl" in telemedicine and has attracted more and more attention. As an extension of traditional surgery, telesurgery greatly extends the connotation and concept of surgery and embodies the great leap forward development of surgical technology. Despite the current limitations such as network delay, transparency of remote robot operation and team construction of surgeons, telesurgery has still formed a variety of innovative application scenarios and achieved rapid development in China in recent years. In view of the uneven distribution of medical resources in China and the epidemic of COVID-19 in the world, this paper puts forward the possible problems and solutions in the development of telesurgery, and looks forward to the feasibility of telesurgery technology in process of shifting the focus of medical and health care down to the community level, channeling resources accordingly.


Subject(s)
COVID-19 , Robotic Surgical Procedures , Robotics , Telemedicine , Delivery of Health Care , Humans
4.
European Journal of Immunology ; 51:241-241, 2021.
Article in English | Web of Science | ID: covidwho-1717637
5.
Sustainability ; 14(3):17, 2022.
Article in English | Web of Science | ID: covidwho-1704310

ABSTRACT

The reasonable distribution of COVID-19 testing facilities is a crucial public necessity to protect the civil right of health and the proper functioning of society in the post-epidemic period. However, most of the current COVID-19 testing facilities are in large hospitals in China, partially overlooking the COVID-19 testing needs of rural dwellers. This paper used shortest-path analysis and the improved potential model to measure the accessibility of current and potential COVID-19 testing facilities, superimposing this with the testing demands of residents, as calculated by the population demand index, so as to comprehensively evaluate the equity of the spatial allocation of the current and potential testing facilities, with a particular focus on Haishu District, Ningbo City, China. The results revealed that the overall accessibility of the current testing facilities in Haishu District was high, while the internal spatial differentiation was considerable. The comprehensive accessibility of testing facilities gradually declined from the downtown areas towards the rural areas. Moreover, roughly half of the rural population needing COVID-19 tests encountered hindrances due to poor access to testing agencies. However, after fully exploiting the potential testing facilities, the comprehensive accessibility of testing facilities was significantly improved, and the inequity in the accessibility to testing facilities was effectively alleviated, which significantly improved the equity of the allocation of testing facilities in Haishu District. The leveraging of current medical facilities to boost the number of testing facilities in rural areas could eliminate the disparity of resource distribution caused by urban and rural binary opposition, and could quickly identify external sources of COVID-19 in rural areas in the post-epidemic period. Moreover, efficient COVID-19 testing combined with the travel records of infection carriers can effectively identify unknown infection cases and obviate large-scale infection outbreaks.

6.
Zhonghua Yi Xue Za Zhi ; 102(3): 216-221, 2022 Jan 18.
Article in Chinese | MEDLINE | ID: covidwho-1633059

ABSTRACT

Objective: To clarify the impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant on the performance of existing molecular diagnostic assays, and investigate the detection ability of clinical laboratories across China. Methods: The first nationwide external quality assessment (EQA) for molecular detection of Delta variant was carried out based on the non-infectious phage virus-like particles samples, which were prepared by genetic engineering methods and distributed to 8 488 laboratories nationwide. The EQA panel was composed of three Delta variant samples (7.5×102, 1.5×103 and 6.0×103 copies/ml), one non-variant weak positive sample and one negative sample. The percentage of agreement (PA) of Delta variant samples with different concentration, the PA of Delta variant and non-variant samples with 7.5×102 copies/ml, the PA of assays used by more than 100 laboratories for Delta variant samples with different concentration and the PA of Delta variant and non-variant samples with 7.5×102 copies/ml were calculated and analyzed. Results: The data from 8 127 laboratories were available for evaluation. The testing capability of 98.77% (8 027/8 127) of the participating laboratories was found to be competent in reporting correct results for all samples. The overall percentage of agreement (OPA), negative percentage of agreement (NPA) and positive percentage of agreement (PPA) of the samples were 99.64% (40 490/40 635), 99.73% (8 105/8 127), 99.62% (32 385/32 508), respectively. With the decrease of the concentration of the samples, the PPA of Delta variant samples decreased. The PPAs were 99.41% and 99.51% for Delta variant and non-variant samples with 7.5×102 copies/ml, respectively, with no statistical difference (P=0.392). The OPA, NPA and PPA of the assays used by more than 100 laboratories were all greater than 98%, and no statistical difference of the PPAs was identified between Delta variant and non-variant samples with 7.5×102 copies/ml (P>0.05). Conclusions: Delta variant fails to impair the performance of current molecular diagnostic assays in China. The clinical laboratories have the same detection capabilities for Delta variant and non-variant samples. However, in certain laboratories, further improvement is required to ensure the accurate detection of weak positive samples.


Subject(s)
COVID-19 , SARS-CoV-2 , Humans , Laboratories
7.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(5): 653-656, 2020 May 10.
Article in Chinese | MEDLINE | ID: covidwho-17429

ABSTRACT

Objective: To understand the characteristics of clusters of COVID-19 cases in Tianjin, and provide epidemiological evidence for the prevention and control of COVID-19. Methods: The data of all the COVID-19 cluster cases in Tianjin, reported by 22 February 2020, were collected to analyze the characteristics of different types of the clusters. Results: A total of 115 COVID-19 cases were reported in 33 clusters in Tianjin included 28 family clusters (71 cases), 1 work place cluster (10 cases), 3 transport vehicle clusters (8 cases) and 1 public place cluster (26 cases). Family clusters were caused by the cases from the working place or public place clusters. Numbers of secondary cases of family clusters was between 1 to 7, the median number was 2. The interval from onset to diagnosis for the first case was longer than those of other cases in the familial clusters (Z=-2.406, P=0.016). The median of incubation period of the public place clusters was 2 days. The intervals from onset to diagnosis were significant different among the family, working place and public place clusters (H=8.843, P=0.012), and also significant differences in onset time among the secondary cases (H=16.607, P=0.000). Conclusions: In the surveillance of COVID-19 epidemic, special attention should be paid to places where clustering are prone to occur, and the epidemiological investigation should be carried out timely to confirm the cluster. To prevent the transmission of COVID-19, the close contacts of the patients should be transferred to an assigned observation place on time for single room isolation. The awareness of COVID-19 prevention is low in some rural areas, reflected by many mass gathering activities and delayed medical care seeking after onset. It is necessary to strengthen the health education and take control measures in early period of epidemic.


Subject(s)
Coronavirus Infections/epidemiology , Pneumonia, Viral/epidemiology , COVID-19 , China/epidemiology , Cluster Analysis , Humans , Pandemics
8.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(5): 638-641, 2020 May 10.
Article in Chinese | MEDLINE | ID: covidwho-8369

ABSTRACT

Objective: To investigate the clinical and epidemiological characteristics of 135 confirmed cases of COVID-19 in Tianjin. Methods: The clinical and epidemiological data of 135 cases of COVID-19 in Tianjin were collected, and the data were analyzed with descriptive method. The factors influencing the severity of the illness were analyzed. Results: Among the 135 COVID-19 cases, 72 were males and 63 were females, the age of the cases was (48.62±16.83) years, and the case fatality rate was 2.22%. Local transmission caused 74.81% of the cases. A total of 33 clusters occurred, involving 85.92% of all COVID-19 cases. The median of the incubation period of COVID-19 was 6.50 days, the average generation interval was 5 days, and the household secondary transmission rate was 20.46%. Fever was the main symptom (78.63%), followed by cough (56.48%). Multivariate regression analysis indicated that age (OR=1.038, 95%CI: 1.010-1.167) and the number of chronic underlying diseases (OR=1.709, 95%CI: 1.052-2.777) were the risk factors of severe illness. Conclusions: Fever was the main symptom at the early phase of COVID-19 in Tianjin, and the local cluster cases accounted for high proportion in confirmed COVID-19 cases reported in Tianjin. Severe illness was prone to occur in people with old age and multi underlying diseases. Strict isolation of close contacts and intensive care of high-risk groups are the main measures to reduce the morbidity and case fatality of COVID-19.


Subject(s)
Coronavirus Infections/diagnosis , Coronavirus Infections/epidemiology , Pneumonia, Viral/diagnosis , Pneumonia, Viral/epidemiology , Adult , Aged , COVID-19 , China/epidemiology , Female , Humans , Male , Middle Aged , Pandemics
9.
Zhonghua Yi Xue Za Zhi ; 100(0): E008, 2020 Feb 19.
Article in Chinese | MEDLINE | ID: covidwho-1257
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