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2.
Carbohydr Polym ; 297: 120032, 2022 Dec 01.
Article in English | MEDLINE | ID: covidwho-2068751

ABSTRACT

The cytokine storm is highly associated with inflammatory-type disease severity and patients' survival. Plant polysaccharides, the main natural phytomedicine source, have a great potential to be an effective drug to treat cytokine storm. Herein we found that a polymeric acemannan (ABPA1) isolated from Aloe Vera Barbadensis extract C (AVBEC) exerted prominent inhibitory effects on inflammation-induced cytokine storm. The results displayed that ABPA1 effectively suppressed LPS-induced proinflammatory cytokines release in vitro. Moreover, ABPA1 treatment alleviated the cytokine storm and tissue damage in LPS- and IAV-induced mouse pneumonia models, and altered the phenotypic balance of macrophages in lung tissues. Functionally, ABPA1 enhanced macrophage M2 polarization and phagocytosis in RAW264.7 cells and inhibited LPS-induced M1 polarization. Mechanistically, ABPA1 enhanced mitochondrial metabolism and OXPHOS through activated PI3K/Akt/GSK-3ß signalling pathway. Overall, our findings suggest that ABPA1 may modulate macrophage activation and mitochondrial metabolism by targeting PI3K/Akt/GSK-3ß signalling pathway, thereby alleviating cytokine storm and inflammation.


Subject(s)
Aloe , Aloe/metabolism , Animals , Cytokine Release Syndrome , Cytokines/metabolism , Glycogen Synthase Kinase 3 beta/metabolism , Lipopolysaccharides/pharmacology , Macrophages , Mannans , Mice , Phosphatidylinositol 3-Kinases/metabolism , Plant Extracts/pharmacology , Polysaccharides/pharmacology , Proto-Oncogene Proteins c-akt/metabolism
3.
J Med Virol ; 2022 Oct 13.
Article in English | MEDLINE | ID: covidwho-2059533

ABSTRACT

Retest-positive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA, as a unique phenomenon among discharged individuals, has been demonstrated to be safe in the community. Still, the underlying mechanism of viral lingering is less investigated. In this study, first, we find that the frequency of viral RNA-positive retesting differs among variants. Higher ratios of viral RNA-positive retest were more frequently observed among Delta (61.41%, 514 of 837 cases) and Omicron (39.53%, 119 of 301 cases) infections than among ancestral viral infection (7.27%, 21 of 289 cases). Second, the tissues where viral RNA reoccurred were altered. Delta RNA reoccurred mainly in the upper respiratory tract (90%), but ancestral virus RNA reoccurred mainly in the gastrointestinal tract (71%). Third, vaccination did not reduce the frequency of viral RNA-positive retests, despite high concentrations of viral-specific antibodies in the blood. Finally, 37 of 55 (67.27%) Delta-infected patients receiving neutralizing antibody therapy become viral RNA retest positive when high concentrations of neutralizing antibodies still patrol in the blood. Altogether, our findings suggest that the presentence of high titers of neutralizing antibodies in the blood is incompetent in clearing residual viral RNA in the upper respiratory tract.

4.
Ann Transl Med ; 10(16): 908, 2022 Aug.
Article in English | MEDLINE | ID: covidwho-2040556

ABSTRACT

Background and Objective: The coronavirus disease of 2019 (COVID-19) is highly infectious and mainly involves the respiratory system, with some patients rapidly progress to acute respiratory distress syndrome (ARDS), which is the leading cause of death in COVID-19 patients. Hence, fully understanding the features of COVID-19-related ARDS (CARDS) and early management of this disease would improve the prognosis and reduce the mortality of severe COVID-19. With the development of recent studies which have focused on CARDS, whether CARDS is "typical" or "atypical" ARDS has become a hotly debated topic. Methods: We searched for relevant literature from 1999 to 2021 published in PubMed by using the following keywords and their combinations: "COVID-19", "CARDS", "ARDS", "pathophysiological mechanism", "clinical manifestations", "prognosis", and "clinical trials". Then, we analyzed, compared and highlighted the differences between classic ARDS and CARDS from all of the aspects above. Key Content and Findings: Classical ARDS commonly occurs within 1 week after a predisposing cause, yet the median time from symptoms onset to CARDS is longer than that of classical ARDS, manifesting within a period of 9.0-12.0 days. Although the lung mechanics exhibited in CARDS grossly match those of classical ARDS, there are some atypical manifestations of CARDS: the severity of hypoxemia seemed not to be proportional to injury of lung mechanics and an increase of thrombogenic processes. Meanwhile, some patients' symptoms do not correspond with the extent of the organic injury: a chest computed tomography (CT) will reveal the severe and diffuse lung injuries, yet the clinical presentations of patients can be mild. Conclusions: Despite the differences between the CARDS and ARDS, in addition to the treatment of antivirals, clinicians should continue to follow the accepted evidence-based framework for managing all ARDS cases, including CARDS.

6.
Virus Res ; 321: 198916, 2022 Nov.
Article in English | MEDLINE | ID: covidwho-2008180

ABSTRACT

Coronavirus subverts the host cell cycle to create a favorable cellular environment that enhances viral replication in host cells. Previous studies have revealed that nucleocapsid (N) protein of the coronavirus porcine epidemic diarrhea virus (PEDV) interacts with p53 to induce cell cycle arrest in S-phase and promotes viral replication. However, the mechanism by which viral replication is increased in the PEDV N protein-induced S-phase arrested cells remains unknown. In the current study, the protein expression profiles of PEDV N protein-induced S-phase arrested Vero E6 cells and thymidine-induced S-phase arrested Vero E6 cells were characterized by tandem mass tag-labeled quantitative proteomic technology. The effect of differentially expressed proteins (DEPs) on PEDV replication was investigated. The results indicated that a total of 5709 proteins, including 20,560 peptides, were identified, of which 58 and 26 DEPs were identified in the PEDV N group and thymidine group, respectively (P < 0.05; ratio ≥ 1.2 or ≤ 0.8). The unique DEPs identified in the PEDV N group were mainly involved in DNA replication, transcription, and protein synthesis, of which 60S ribosomal protein L18 (RPL18) exhibited significantly up-regulated expression in the PEDV N protein-induced S-phase arrested Vero E6/IPEC-J2 cells and PEDV-infected IPEC-J2 cells (P < 0.05). Further studies revealed that the RPL18 protein could significantly enhance PEDV replication (P < 0.05). Our findings reveal a mechanism regarding increased viral replication when the PEDV N protein-induced host cells are in S-phase arrest. These data also provide evidence that PEDV maintains its own replication by utilizing protein synthesis-associated ribosomal proteins.


Subject(s)
Coronavirus Infections , Porcine epidemic diarrhea virus , Swine Diseases , Animals , Chlorocebus aethiops , Porcine epidemic diarrhea virus/genetics , Proteomics/methods , Ribosomal Proteins/genetics , Ribosomal Proteins/metabolism , Swine , Thymidine/metabolism , Tumor Suppressor Protein p53/metabolism , Vero Cells , Virus Replication
7.
Cell Discov ; 8(1): 70, 2022 Jul 25.
Article in English | MEDLINE | ID: covidwho-1960340

ABSTRACT

Little is known regarding why a subset of COVID-19 patients exhibited prolonged positivity of SARS-CoV-2 infection. Here, we found that patients with long viral RNA course (LC) exhibited prolonged high-level IgG antibodies and higher regulatory T (Treg) cell counts compared to those with short viral RNA course (SC) in terms of viral load. Longitudinal proteomics and metabolomics analyses of the patient sera uncovered that prolonged viral RNA shedding was associated with inhibition of the liver X receptor/retinoid X receptor (LXR/RXR) pathway, substantial suppression of diverse metabolites, activation of the complement system, suppressed cell migration, and enhanced viral replication. Furthermore, a ten-molecule learning model was established which could potentially predict viral RNA shedding period. In summary, this study uncovered enhanced inflammation and suppressed adaptive immunity in COVID-19 patients with prolonged viral RNA shedding, and proposed a multi-omic classifier for viral RNA shedding prediction.

8.
Int J Biol Sci ; 18(12): 4618-4628, 2022.
Article in English | MEDLINE | ID: covidwho-1954686

ABSTRACT

This study aimed to explore the clinical practice of phospholipid metabolic pathways in COVID-19. In this study, 48 COVID-19 patients and 17 healthy controls were included. Patients were divided into mild (n=40) and severe (n=8) according to their severity. Phospholipid metabolites, TCA circulating metabolites, eicosanoid metabolites, and closely associated enzymes and transfer proteins were detected in the plasma of all individuals using metabolomics and proteomics assays, respectively. 30 of the 33 metabolites found differed significantly (P<0.05) between patients and healthy controls (P<0.05), with D-dimmer significantly correlated with all of the lysophospholipid metabolites (LysoPE, LysoPC, LysoPI and LPA). In particular, we found that phosphatidylinositol (PI) and phosphatidylcholine (PC) could identify patients from healthy controls (AUC 0.771 and 0.745, respectively) and that the severity of the patients could be determined (AUC 0.663 and 0.809, respectively). The last measurement before discharge also revealed significant changes in both PI and PC. For the first time, our study explores the significance of the phospholipid metabolic system in COVID-19 patients. Based on molecular pathway mechanisms, three important phospholipid pathways related to Ceramide-Malate acid (Cer-SM), Lysophospholipid (LPs), and membrane function were established. Clinical values discovered included the role of Cer in maintaining the inflammatory internal environment, the modulation of procoagulant LPA by upstream fibrinolytic metabolites, and the role of PI and PC in predicting disease aggravation.


Subject(s)
COVID-19 , Disease Progression , Humans , Lysophospholipids , Metabolome , Metabolomics
9.
Nat Commun ; 13(1): 3979, 2022 07 09.
Article in English | MEDLINE | ID: covidwho-1927086

ABSTRACT

Despite timely immunization programs, and efficacious vaccines conveying protection against SARS-CoV-2 infection, breakthrough infections in vaccinated individuals have been reported. The Delta variant of concern (VOC) outbreak in Guangzhou resulted in local transmission in vaccinated and non-vaccinated residents, providing a unique opportunity to study the protective effects of the inactivated vaccines in breakthrough infection. Here, we find that the 2-dose vaccinated group has similar peak viral titers and comparable speeds of viral RNA clearance to the non-vaccinated group but accelerated viral suppression in the middle course of the disease. We quantitatively demonstrate that peak viral pneumonia is significantly mitigated in the 2-dose vaccine group (median 0.298%) compared with the non-vaccinated (5.77%) and 1-dose vaccine (3.34%) groups. Pneumonia absorbance is approximately 6 days ahead in the 2-dose group (median 10 days) than in the non-vaccinated group (16 days) (p = 0.003). We also observe reduced cytokine inflammation and markedly undisturbed gene transcription profiles of peripheral blood mononuclear cells (PBMCs) in the 2-dose group. In short, our study demonstrates that prior vaccination substantially restrains pneumonia development, reduces cytokine storms, and facilitates clinical recovery.


Subject(s)
COVID-19 , Viral Vaccines , COVID-19/prevention & control , Humans , Leukocytes, Mononuclear , SARS-CoV-2 , Vaccination
10.
Chin Med ; 17(1): 77, 2022 Jun 21.
Article in English | MEDLINE | ID: covidwho-1896362

ABSTRACT

OBJECTIVE: To systematically review the clinical efficacy and safety of Chinese herbal medicine (CHM) with and without Western medicine (WM) for different severity of COVID-19. METHODS: CNKI, PubMed, Wanfang Database, ClinicalTrails.gov, Embase, ChiCTR and ICTRP were searched from 01 Jan, 2020 to 30 Jun, 2021. Two authors independently assessed all the randomized clinical trials (RCTs) for trial inclusion, data extraction and quality assessment. Meta-analysis was conducted using Review Manager software (RevMan 5.4.1). Evidence was assessed using Grading of Recommendations Assessment, Development, and Evaluation (GRADE). Primary outcomes included total effectiveness rate. Secondary outcomes included improvements in symptom improvement and total adverse event rate. Different severity of COVID-19 patients was assessed in subgroup analysis. This study was registered with INPLASY, INPLASY202210072. RESULTS: 22 high quality RCTs involving 1789 participants were included. There were no trial used CHM alone nor compare placebo or no treatment. Compared with WM, combined CHM and WM (CHM-WM) treatment showed higher total effectiveness rate, lower symptom scores of fever, cough, fatigue, dry throat and pharyngalgia, shorter mean time to viral conversion, better Computerized Tomography (CT) image and blood results, fewer total adverse events and worse conditions (P < 0.05). Subgroup analysis showed that the total effectiveness rate of combined CHM-WM group was significantly higher than WM group, especially for mild and moderate patients. No significant differences in mortality and adverse events were found between combined CHM-WM and WM treatment. No serious adverse events and long-term outcomes were reported. CONCLUSION: Current evidence supported the therapeutic effects and safety of combined CHM-WM treatment on COVID-19, especially for patients with mild and moderate symptoms. Long-term effects of therapy are worthy in further study.

11.
Front Psychol ; 13: 801006, 2022.
Article in English | MEDLINE | ID: covidwho-1887127

ABSTRACT

In the context of the COVID-19, we examined the relationship between college students' ego depletion and their prosocial behavior. We explored the mediating role of social self-efficacy between ego depletion and prosocial behavior, we also examined the moderating role of personal belief in a just world in this relationship. 1,122 college students completed the ego depletion questionnaire, prosocial behavior questionnaire, social self-efficacy questionnaire, and personal belief in a just world questionnaire. The current findings suggested that: (1) Social self-efficacy mediated the relationship between college students' ego depletion and their prosocial behaviors. The ego depletion of college students could be used to predict their prosocial behavior through social self-efficacy. (2) Personal belief in a just world moderated the relationship between social self-efficacy and prosocial behavior.

12.
Journal of Radiation Research and Applied Sciences ; 2022.
Article in English | ScienceDirect | ID: covidwho-1851648

ABSTRACT

The study aimed to determine the trends in the manifestations and severity over the epidemic course of imported COVID-19 cases, with comparison to native cases. The clinical characteristics of imported and native Chinese COVID-19 cases included in the study were assessed and compared. The association was analyzed using Mann-Whitney U test for categorical variables, Kruskal-Wallis H test for continuous variables, and Spearman’s correlation test for disease severity. A total of 247 imported patients were enrolled, with an average age of 29 years, and 41.3% were female. The imported patients were younger than the native patients (29 vs 47 years) and included a lower proportion of fever (44.1%), chills (5.3%), fatigue (10.1%), leukopenia (14.6%), lymphopenia (39.3%), elevated C-reactive protein (CRP) (7.3%), elevated D-dimer (16.3%), and pneumonia (65.6%). Among patients with moderate severity, imported cases had a lower proportion of fever (44.2%), dyspnea (8.3%), and increased CRP (7.7%) than native cases. COVID-19 infection was less severe in imported cases than that in native cases, reflected by fewer clinical symptoms, fewer comorbidities, and lower overall severity.

13.
Acta Psychol (Amst) ; 227: 103617, 2022 Jul.
Article in English | MEDLINE | ID: covidwho-1850538

ABSTRACT

To investigate the relationship between stress response and depression in vocational medical school students at the initial stage of COVID-19 epidemic, and to explore the mediating role of meaning in life and the moderating role of prosocial behavior. The COVID-19 Stress Response Questionnaire, the Meaning in Life Questionnaire, the Prosocial Behavior Scale and the Depression Scale, were used to investigate 3526 vocational medical school students. The results showed that: (1) The relationship between stress response and depression in vocational medical school students was partially mediated by presence of meaning and search for meaning. Stress response has indirect effect on depression of vocational medical school students through meaning in life. (2) Prosocial behavior moderated the relationship between meaning in life and depression. Specifically, meaning in life has a more significant effect on depression for college students with higher levels of prosocial behavior.


Subject(s)
COVID-19 , COVID-19/epidemiology , Humans , Schools, Medical , Students , Surveys and Questionnaires
14.
ACS omega ; 7(16):14211-14221, 2022.
Article in English | EuropePMC | ID: covidwho-1836996

ABSTRACT

The current global pandemic of new coronary pneumonia clearly reveals the importance of developing highly efficient filtration and fast germicidal performance of multifunctional air filters. In this study, a novel air filter with a controllable morphology based on the rod-like to flower-like zinc oxide/graphene-based photocatalytic composite particles loaded on glass microfiber was prepared by one-step microwave rapid synthesis. The multifunctional air filter shows the following special functions: the 10 mg·L–1 organic pollutant solution RhB was completely degraded within 2 h under a 500 W xenon lamp, and also 99% of Escherichia coli and Staphylococcus aureus were inactivated under a 60 W light-emitting diode lamp. Furthermore, after introducing the controllable morphology zinc oxide/graphene-based photocatalytic composite particles, the filtration efficiency of the multifunctional air filter was also kept at the same level (99.8%) as the one without any addition, indicating no loss of high-efficiency filtration while obtaining the rapid bactericidal function. The rapid antibacterial principle of the multifunctional air filter has also been proposed through the UV–vis spectroscopies, photoluminescence, and electron-spin resonance spectrum. The zinc oxide/graphene-based photocatalytic composite particles tightly coated on the glass microfiber surface could increase the active sites by changing the morphology of zinc oxide and, in the meantime, promote the separation of zinc oxide photo-generated electron–hole pairs to improve the rapid sterilization ability of the multifunctional air filters. In addition, an empirical formula to evaluate the relationship between the composition, viscosity, and viscosity modulus of glass microfiber was proposed by testing the viscosity of glass microfiber composed of 14 different compositions at 1300 and 1400 °C, which can be used as a criterion to evaluate the production technology of glass microfiber filters.

15.
Viruses ; 14(5)2022 05 05.
Article in English | MEDLINE | ID: covidwho-1820426

ABSTRACT

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2, SARS2) remains a great global health threat and demands identification of more effective and SARS2-targeted antiviral drugs, even with successful development of anti-SARS2 vaccines. Viral replicons have proven to be a rapid, safe, and readily scalable platform for high-throughput screening, identification, and evaluation of antiviral drugs against positive-stranded RNA viruses. In the study, we report a unique robust HIV long terminal repeat (LTR)/T7 dual-promoter-driven and dual-reporter firefly luciferase (fLuc) and green fluorescent protein (GFP)-expressing SARS2 replicon. The genomic organization of the replicon was designed with quite a few features that were to ensure the replication fidelity of the replicon, to maximize the expression of the full-length replicon, and to offer the monitoring flexibility of the replicon replication. We showed the success of the construction of the replicon and expression of reporter genes fLuc and GFP and SARS structural N from the replicon DNA or the RNA that was in vitro transcribed from the replicon DNA. We also showed detection of the negative-stranded genomic RNA (gRNA) and subgenomic RNA (sgRNA) intermediates, a hallmark of replication of positive-stranded RNA viruses from the replicon. Lastly, we showed that expression of the reporter genes, N gene, gRNA, and sgRNA from the replicon was sensitive to inhibition by Remdesivir. Taken together, our results support use of the replicon for identification of anti-SARS2 drugs and development of new anti-SARS strategies targeted at the step of virus replication.


Subject(s)
Replicon , SARS-CoV-2 , Antiviral Agents/pharmacology , Genes, Reporter , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Luciferases, Firefly/genetics , Luciferases, Firefly/metabolism , Promoter Regions, Genetic , RNA, Guide , SARS-CoV-2/genetics , SARS-CoV-2/physiology , Virus Replication/drug effects
16.
Int J Ment Health Addict ; : 1-15, 2021 Nov 11.
Article in English | MEDLINE | ID: covidwho-1772993

ABSTRACT

During the COVID-19 pandemic, mobile phone dependence (MPD) became a common addictive behavior among adolescents because of social isolation. To understand the underlying mechanisms of the impact of social isolation on MPD, this study examined the mediating role of loneliness and the moderating role of parent-child communication patterns on social isolation and MPD. Six hundred and eighty-three adolescents from a middle school in Huang'gang city, China, were recruited to complete a self-report questionnaire. Data analysis comprised latent profile analysis and structural equation modelling. The results showed that social isolation had a significant and positive indirect effect on MPD via loneliness. In addition, three types of parent-child communication patterns were observed: complex, adaptive, and maladaptive. For adolescents with complex parent-child communication, the indirect relation of social isolation to MPD via loneliness is weaker than that of adolescents with maladaptive parent-child communication. These findings suggested that the indirect effect of social isolation on MPD via loneliness was moderated by parent-child communication patterns. Psychological intervention for MPD should emphasize relieving adolescents' loneliness and improving the quality of  their communication with parents during the COVID-19 pandemic.

17.
EuropePMC; 2021.
Preprint in English | EuropePMC | ID: ppcovidwho-324298

ABSTRACT

Background: The outbreak of Coronavirus Disease 2019(COVID-19) caused psychological stress in Chinese adults population. But we are unaware of whether the pandemic causes psychological stress on children. Methods: : We used the Children’s Impact of Event Scale questionnaire (CRIES-13) to investigate the degree of Post-traumatic Stress (PTSD) symptoms caused by the pandemic in students selected from schools in Sichuan, Jiangsu, Henan, Yunnan, and Chongqing provinces of China. Results: : A total of 7769 students(3692 male and 4077 female), aged 8-18 years, were enrolled in the study, comprising 1214 in primary schools, 2799 in junior high schools and 3756 in senior high schools. A total of 1639 students (21.1%) had severe psychological stress reactions. A large proportion of senior high school students (23.3%) experienced severe psychological stress, and they had the highest median total CRIES-13 score. Female students were more likely to experience severe psychological stress and had higher median CRIES-13 total scores than males. Conclusion: COVID-19 has placed psychological stresses on primary and secondary school students in China. These stresses are more likely to reach severe levels among female students and senior high school students.

18.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-324125

ABSTRACT

Abstract Objective To understand the mental health status and needs of the health care workers during the epidemic period of COVID-19, so as to provide scientific basis for the targeted intervention measures. Methods A total of 511 health care workers were randomly selected from two designated tuberculosis medical institutions in Anhui province. Results There were 139 people in epidemic prevention and control positions (27.20%). Depression level: female was higher than male;nurse was higher than doctor;middle and junior job titles were higher than senior titles;junior college degree or below were higher than bachelor's degree, master's degree and above;isolation ward, fever clinic and pre-check triage were significantly higher than those of non-prevention and control positions ( P < 0.05). Loneliness scores: doctors were higher than that of medical technicians, and isolation ward, fever clinic and pre-check triage were higher than those of non-prevention and control positions ( P < 0.05). Social support: doctors were lower than that of medical technicians, and isolation ward, fever clinic and pre-check triage were significantly lower than those of non-prevention and control positions ( P < 0.05). The score of social support was negatively correlated with depression and loneliness ( P < 0.001), while depression was positively correlated with loneliness ( P < 0.001). Health care workers most want to receive one-to-one psychological counseling (29.75%), and provide crisis management (24.07%). The awareness rate of health care workers on COVID-19's knowledge was relatively high. Conclusions The psychological problems of health care workers, especially women, nurses with low educational background, low professional title, and staff in the epidemic prevention and control positions are relatively serious. We should focus on this population, and take targeted intervention measures.

19.
EuropePMC; 2021.
Preprint in English | EuropePMC | ID: ppcovidwho-323598

ABSTRACT

Objective: . To understand the difficulties and survival strategies in nursing during NCP outbreak, and to reflect and summarize the experience. Background: . Since December 2019, the highly infectious novel coronavirus pneumonia overwhelmed health care systems and medical workers who had to provide care in situations involving high personal risk and stress, some becoming infected and dying. Nurse leaders had to develop new strategies for nursing care. Methods: . Using the phenomenological research method in qualitative research, 8 head nurses who participated in NCP treatment were interviewed in-depth, and then Colaizzi 7-step analysis method was used to summarize. Results: . Working under great pressure, nursing leaders led the team through a period of crisis: shock and fear, learning in chaos, supporting nurses, and rewarding nurses. Conclusion: . As important intervention performers in the crisis, nurse leaders need to have their own outstanding leadership to effectively manage internal conflicts and interpersonal relationships, strengthen teamwork training and establish supportive system so as to better deal with the management of similar public health events in the future. Relevance to clinical practice . Findings will assist nurse leaders to prepare themselves in the outbreak. It is hoped that the results of this study will contribute to disaster management in similar infectious outbreaks in the future.

20.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-315953

ABSTRACT

Background: Covid-19 vaccine research and development is progressing and expected to be put into use in a predictable time, we aimed to learn the awareness and acceptance of the new vaccine by healthcare workers (HCWs) in Beijing, China. Methods: : A cross-sectional survey was conducted to investigate HCWs including doctors, nurses and technicians from sixty hospitals in Beijing to obtain the perception of COVID-19 epidemic and the attitudes towards vaccination before before the completion of vaccine phase III clinical trials. Multivariate analysis was applied to evaluate the associated factors with intention to get vaccination. Results: : A total of 8040 HCWs was recruited. 67.1% reported they would get vaccination, others said unsure or would not. Half of the HCWs were unsure whether the outbreak in China would come back and the global epidemic would last for a long time. 67.6% agreed the epidemic can be prevented by vaccination. Positive associated factors with willingness to get vaccination were mainly included epidemic situation prognosis, perception of disease severity, self infection risk and disease can be prevented by vaccine, etc. Two positive factors of “wanted the vaccine free of charge” (OR:5.807, 95%CI:5.083-6.635, P<0.001) and “believed vaccine approved for license have been fully evaluated in clinical trials” (OR:4.485, 95%CI:3.849-5.227, P<0.001) were strongly associated with willingness to get vaccination, while two factors of “highest academic degree” (OR:0.840, 95%CI:0.772-0.914, P<0.001) and “professional ranks and titles” (OR:0.930, 95%CI:0.865-1.000, P=0.049) were negative associated . Conclusions: : A little above moderate willingness to get COVID-19 vaccination was found among HCWs in Beijing before the vaccine being licensed. Free vaccination strategy should be considered to implement, effective measures should be taken to remove barriers and convey correct information through appropriate ways to enhance the acceptance of COVID-19 vaccination among HCWs in China.

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