Your browser doesn't support javascript.
Show: 20 | 50 | 100
Results 1 - 20 de 68
Filter
1.
Mol Psychiatry ; 2022 Jun 06.
Article in English | MEDLINE | ID: covidwho-1878515

ABSTRACT

The long-term physical and mental sequelae of COVID-19 are a growing public health concern, yet there is considerable uncertainty about their prevalence, persistence and predictors. We conducted a comprehensive, up-to-date meta-analysis of survivors' health consequences and sequelae for COVID-19. PubMed, Embase and the Cochrane Library were searched through Sep 30th, 2021. Observational studies that reported the prevalence of sequelae of COVID-19 were included. Two reviewers independently undertook the data extraction and quality assessment. Of the 36,625 records identified, a total of 151 studies were included involving 1,285,407 participants from thirty-two countries. At least one sequelae symptom occurred in 50.1% (95% CI 45.4-54.8) of COVID-19 survivors for up to 12 months after infection. The most common investigation findings included abnormalities on lung CT (56.9%, 95% CI 46.2-67.3) and abnormal pulmonary function tests (45.6%, 95% CI 36.3-55.0), followed by generalized symptoms, such as fatigue (28.7%, 95% CI 21.0-37.0), psychiatric symptoms (19.7%, 95% CI 16.1-23.6) mainly depression (18.3%, 95% CI 13.3-23.8) and PTSD (17.9%, 95% CI 11.6-25.3), and neurological symptoms (18.7%, 95% CI 16.2-21.4), such as cognitive deficits (19.7%, 95% CI 8.8-33.4) and memory impairment (17.5%, 95% CI 8.1-29.6). Subgroup analysis showed that participants with a higher risk of long-term sequelae were older, mostly male, living in a high-income country, with more severe status at acute infection. Individuals with severe infection suffered more from PTSD, sleep disturbance, cognitive deficits, concentration impairment, and gustatory dysfunction. Survivors with mild infection had high burden of anxiety and memory impairment after recovery. Our findings suggest that after recovery from acute COVID-19, half of survivors still have a high burden of either physical or mental sequelae up to at least 12 months. It is important to provide urgent and appropriate prevention and intervention management to preclude persistent or emerging long-term sequelae and to promote the physical and psychiatric wellbeing of COVID-19 survivors.

2.
J Med Internet Res ; 24(5): e32922, 2022 05 30.
Article in English | MEDLINE | ID: covidwho-1875274

ABSTRACT

BACKGROUND: Considered a facet of behavioral impulsivity, response inhibition facilitates adaptive and goal-directed behavior. It is often assessed using the Stop-Signal Task (SST), which is presented on stand-alone computers under controlled laboratory conditions. Sample size may consequently be a function of cost or time and sample diversity constrained to those willing or able to attend the laboratory. Statistical power and generalizability of results might, in turn, be impacted. Such limitations may potentially be overcome via the implementation of web-based testing. OBJECTIVE: The aim of this study was to investigate if there were differences between variables derived from a web-based SST when it was undertaken independently-that is, outside the laboratory, on any computer, and in the absence of researchers-versus when it was performed under laboratory conditions. METHODS: We programmed a web-based SST in HTML and JavaScript and employed a counterbalanced design. A total of 166 individuals (mean age 19.72, SD 1.85, range 18-36 years; 146/166, 88% female) were recruited. Of them, 79 undertook the independent task prior to visiting the laboratory and 78 completed the independent task following their laboratory visit. The average time between SST testing was 3.72 (SD 2.86) days. Dependent samples and Bayesian paired samples t tests were used to examine differences between laboratory-based and independent SST variables. Correlational analyses were conducted on stop-signal reaction times (SSRT). RESULTS: After exclusions, 123 participants (mean age 19.73, SD 1.97 years) completed the SST both in the laboratory and independently. While participants were less accurate on go trials and exhibited reduced inhibitory control when undertaking the independent-compared to the laboratory-based-SST, there was a positive association between the SSRT of each condition (r=.48; P<.001; 95% CI 0.33-0.61). CONCLUSIONS: Findings suggest a web-based SST, which participants undertake on any computer, at any location, and in the absence of the researcher, is a suitable measure of response inhibition.


Subject(s)
Inhibition, Psychological , Psychomotor Performance , Adolescent , Adult , Bayes Theorem , Female , Humans , Internet , Male , Psychomotor Performance/physiology , Reaction Time/physiology , Young Adult
3.
J Affect Disord ; 311: 40-46, 2022 08 15.
Article in English | MEDLINE | ID: covidwho-1851381

ABSTRACT

BACKGROUND: Sustained stress during COVID-19 may be associated with depression in front-line medical staff, which would expose them to severe threats. This study aimed to examine whether the relationship between perceived stress and depression is mediated by insomnia, and whether this mediation is moderated by resilience. METHODS: For front-line medical staff, this study used online questionnaire to evaluate their perceived stress, depression, insomnia and resilience. A conditional process model was performed to examine the relationship between perceived stress and depression, as well as the mediating effect of insomnia and the moderating effect of resilience. RESULTS: A total of 606 front-line medical staff completed the survey. Higher level of perceived stress was significantly positively related to severe insomnia and depression. In addition, insomnia was positively related to depression, while resilience could moderate the effect of perceived stress on depression by direct and indirect paths. LIMITATIONS: The causality among perceived stress, depression, insomnia and resilience is difficult to be verified. CONCLUSIONS: Perceived stress is positively related to depression, and insomnia can mediate the effect of perceived stress on depression. In addition, the effect of perceived stress on depression, whether direct or indirect, is moderated by resilience, which is a protective factor for mental health.


Subject(s)
COVID-19 , Resilience, Psychological , Sleep Initiation and Maintenance Disorders , China/epidemiology , Cross-Sectional Studies , Depression/epidemiology , Depression/psychology , Humans , Medical Staff , Sleep Initiation and Maintenance Disorders/epidemiology , Sleep Initiation and Maintenance Disorders/etiology , Stress, Psychological/epidemiology , Stress, Psychological/psychology
6.
EuropePMC; 2022.
Preprint in English | EuropePMC | ID: ppcovidwho-331100

ABSTRACT

Under the growing crisis of coronavirus disease 2019 pandemic, the global medical system is facing the predicament of an acute shortage of medical-grade oxygen (O 2 , ≥ 99.5% purity). Herein, we manufactured an oxygen generation device relying on electrochemical technology. The performance of electrochemical oxygen generator (EOG) was remarkably improved to a practically applicable level, achieving long-term (>200 h), stable, and quick production (>1.5L/min) of high purity O 2 (99.9%) under high energy efficiency (496 L/kW·h), viasimultaneous optimizations for intrinsic electrochemical reaction mechanism, electrocatalysts, and external cell structure. The EOG also presents powerful competitiveness in user experience, which finds expression in high portability (4.7 kg), nearly instant O 2 production (<1 s), and a quiet working condition (<39 dB). The EOG shows great potential to substitute commercial pressure swing adsorption O 2 generation devices, which may significantly impact the traditional oxygen production industry.

7.
Ann Transl Med ; 10(4): 232, 2022 Feb.
Article in English | MEDLINE | ID: covidwho-1716328

ABSTRACT

Since 2019, new coronavirus pneumonia has been widespread worldwide and has had a direct impact on human life. Meanwhile, it also has a huge impact on teaching in universities and hospitals, especially in experimental courses. Cell biology is not only an important part of biology but also one of the most dynamic frontier branches of modern life science, which is also a science based on experiments. It is necessary to promote the online and offline teaching during this special period. To explore and evaluate the blended teaching modes during the COVID-19 epidemic, we changed the previous single teaching mode of "teaching + experiment" and published courseware and learning requirements online before class, this kind of mode takes "theory + virtual demonstration experiment" in class as the main body and the reviewing and thinking after class as the summary. We also carried out offline practice on a small scale when the epidemic allowed and actively explored the comprehensive online and offline teaching modes, in and out of class. Through the hybrid teaching, the percentage distribution of students in high and moderate scores ranges were greatly increased, the blended teaching mode also greatly improves students' subjective initiative and highlights the development of students' personalized thinking and scientific research ability. The practice of this model not only ensures the health of teachers and students during the epidemic but also provides a new teaching exploration model and thinking for the development of experimental courses during the epidemic.

8.
ISPRS International Journal of Geo-Information ; 11(3):152, 2022.
Article in English | MDPI | ID: covidwho-1704039

ABSTRACT

Exploring the spatial patterns of COVID-19 transmission and its key determinants could provide a deeper understanding of the evolution of the COVID-19 pandemic. The goal of this study is to investigate the spatial patterns of COVID-19 transmission in different periods in Singapore, as well as their relationship with demographic and built-environment factors. Based on reported cases from 23 January to 30 September 2020, we divided the research time into six phases and used spatial autocorrelation analysis, the ordinary least squares (OLS) model, the multiscale geographically weighted regression (MGWR) model, and dominance analysis to explore the spatial patterns and influencing factors in each phase. The results showed that the spatial patterns of COVID-19 cases differed across time, and imported cases presented a random pattern, whereas local cases presented a clustered pattern. Among the selected variables, the supermarket density, elderly population density, hotel density, business land proportion, and park density may be particular fitting indicators explaining the different phases of pandemic development in Singapore. Furthermore, the associations between determinants and COVID-19 transmission changed dynamically over time. This study provides policymakers with valuable information for developing targeted interventions for certain areas and periods.

9.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-319968

ABSTRACT

Background: The outbreak of novel 2019 coronavirus disease (COVID-19) has imposed an enormous physical and psychological pressure on people across the world. This study focused on evaluating the prevalence and influencing factors of anxiety and depression symptoms in surgical nurses during the epidemic in China. MethodA cross-sectional, multicenter quantitative study was conducted in Anhui province (China) from March 3, 2020 to March 19, 2020, with a questionnaire package which consisted of general information questionnaire,Zung's self-rating anxiety scale (SAS), Zung's self-rating Depression Scale (SDS) and social support rating scale (SSRS). A total of 3600 surgical nurses participated in the survey by Wechat and QQ. Data were analysed using multiple linear regression models. ResultsA total of 3492 surgical nurses from 12tertiary hospitals and 12 secondary hospitals in one province of mainland China completed the survey. The prevalence rates of anxiety symptoms and depressive symptoms were 24.83% and 22.39%, respectively. The average level of anxiety and depression of surgical nurses were higher than that of the Chinese norm ( P < 0.05).Levels of social support for surgical nurses were significantly negatively associated with the degree of anxiety (r = -0.630, P < 0.001) and depression (r = -0.578, P < 0.001). Fertility status ( β = 1.469, P = 0.003), hospital ( β = -0.611, P < 0.001), participation in care for COVID-19 patients ( β = 2.229, P < 0.001), likelihood of being infected with COVID-19 ( β = 1.146, P < 0.001), social support ( β = -0.623, P < 0.001) were significantly influencing surgical nurses’ anxiety degree. Similarly, these characteristics were significantly associated with the odds of experiencing depression symptoms in surgical nurses. Divorce and widowed surgical nurses ( β = -2.654, P < 0.001) were significantly more likely to experience depressive symptoms than single nurses. ConclusionIn this survey, we found that the surgical nurses had high anxiety and depression symptoms during the COVID-19 outbreak in China. The findings suggest that targeted psychological interventions to promote the mental health of surgical nurses with psychological problems need to be immediately implemented.

10.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-315626

ABSTRACT

The therapeutic effect of tocilizumab remains controversial. We aimed to evaluate whether tocilizumab might be beneficial in COVID-19 patients. We searched PubMed, Embase and Cochrane library from inception to June 23, 2020. Summary estimates of overall response rate (ORR) and all-cause death rate in all patients were analyzed. This study was registered with PROSPERO (CRD42020191313). We included data from 28 articles including 991 COVID-19 patients who underwent tocilizumab administration. The pooled ORR was 72% (95% CI, 66-79%) and pooled all-cause death rate was 16% (95% CI, 11-22%). The optimal timing of administration was the 7.15 day from the symptom onset and with the lowest death rate of 13.11%. 562 patients were defined as with severe infection, and the pooled ORR was 78% (95% CI, 70-85%). The pooled ORR of 56 organ transplantation recipients was 53% (95% CI, 26-78%), which was lower than non-transplant patients [75% (95% CI, 69-81%)]. Nearly all studies confirmed the safety of tocilizumab administration. Tocilizumab improves the clinical outcome of COVID-19 patients, especially in severe cases, and the optimal timing of administration may provide the guidance for management. However, tocilizumab may be used with caution in solid transplant recipients for the suboptimal efficacy.

11.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-315595

ABSTRACT

Background: Since the first public genome of SARS-CoV-2, over 170,000 genome sequences of the virus have been shared by researchers world-wide (till November 1st 2020). Multiplex PCR targeting SARS-CoV-2 followed by massively parallel sequencing (MPS) and/or nanopore sequencing is a widely used strategy to recover the genome from primary samples. However, the bias of amplification among different amplicons should not be ignored, which might lead to uneven sequencing coverage on the viral genome.MethodsWe aim to develop a novel multiplex PCR panel to achieve an improved coverage evenness of SARS-CoV-2. We adapt long amplicons (~1000-bp) for the panel and thus reduced the number of primer pairs. The panel was validated with clinical samples and sequenced via MPS sequencing systems and a portable nanopore sequencing device MinION. We evaluated the full-genome coverage evenness and its dependence on viral loads of the long amplicon panel;we then compared it with a 98-plex panel provided by the ARTIC network. The accuracy to identify viral genomic variations based on the panel and sequencing with MinION was assessed.ResultsWe developed a two-pool 36-plex panel for full-genome sequencing of SARS-CoV-2, whose amplicon size ranged from 880 to 1027 bp. For samples with a <30 C t value, >90% viral genome could be recovered with a high sequencing depth (>0.2 mean depth) by using the long-amplicon panel (n = 36), compared with 79-88% highly covered genome region for the ARTIC panel (n = 5). The coverage evenness of the long-amplicon panel was also less affected by low viral titers and not dependent on sequencing data amount. With MinION sequencing, the consensus viral genomes could be reliably recovered. However, a high false positive rate was observed to identify sub-clonal genomic variations with a <0.6 frequency.ConclusionA novel multiplex PCR panel for full-genome sequencing of SARS-CoV-2 with improved coverage evenness and low requirement of data throughput was validated with clinical samples. Amplification of SARS-CoV-2 with the panel followed by MinION sequencing could generate reliable consensus genome sequences, but the detection of non-dominating viral populations within host is error-prone.

12.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-313360

ABSTRACT

The resurgence of coronavirus disease 2019 (COVID-19) has been seen in many counties where outbreaks appear to be leveling off. While China experienced a dramatic decline of COVID-19 at the outset of 2020, regional outbreaks continuously emerged in recent months. In Guangzhou, a small outbreak emerged in March and April involving less than 100 residents, and a comprehensive and near-real-time genomic surveillance of SARS-CoV-2 was conducted. When confirmed cases among overseas travelers increased, public health authorities enhanced measures as shifting self-quarantine to central quarantine and SARS-CoV-2 testing for all overseas travelers. From 109 imported cases we found diverse viral variants distributing in the global viral phylogeny, which were usually shared within households but not among passengers on the same flight. Nonetheless, local transmission was predominately attributed to two specific variants imported from Africa, including the local cases who reported no direct/indirect contact with imported cases. The introducing events of the virus were identified or deduced before enhanced measures were taken. These results show that the interventions are effective in containing the spread of SARS-CoV-2, and also rule out the possibility of cryptic transmission of viral variants from the first wave in January and February. Moreover, we found that intra-host viral diversity was usually different between close contacts, implying a transmission bottleneck of SARS-CoV-2. Our study provides evidence and emphasizes the importance of controls for oversea travelers in the context of the pandemic, and exemplifies how viral genomic data facilitates COVID-19 surveillance and prevention.Funding: This study was supported by National Natural Science Foundation of China (31870079, 91953122, 31871326), National Science and Technology Major Project of the Ministry of Science and Technology of China (2017ZX10103011, 2018ZX10305410, 2018ZX10201001), Guangdong Provincial Novel Coronavirus Scientific and Technological Project (2020111107001), Guangdong Basic and Applied Basic Research Foundation (2020A1515010776 and 2020B1515020057) and the Beijing Nova Program (Z181100006218114 and Z181100006218110) to M.N. and P.L..Conflict of Interest: The authors declare no competing interests.Ethical Approval: This study was approved by the ethics committee of the Center for Disease Control and Prevention (CDC) of Guangzhou (GZCDC-ECHR-2020P0002). Written informed consent was obtained from patients about the surveillance and data related to disease control and further analysis. All information regarding individual persons has been anonymized in this study.

13.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-309023

ABSTRACT

Background: The COVID-19 pandemic is a major health crisis has led to adverse mental health consequences in the general public, medical staff, and individual in self isolation. In order to stop transmission of the virus and save lives, Fangcang shelter hospitals were developed and used for the first time in China. However, there is no research on mental health problems in Fangcang shelter hospitals patients during the COVID-19 outbreak. The aim of this study was to survey the prevalence and major influencing factors of anxiety, depression among the hospitalized Coronavirus Disease 2019 (COVID-19) cases in Fangcang shelter hospital. Methods From February 23rd, 2020, to February 26th, 2020, we obtained the information of demographic data, clinical symptoms, and assessed the mental health status, sleep quality by using an online questionnaire including self-rating anxiety scale (SAS), self-rating depressive scale (SDS) and pittsburgh sleep quality index (PSQI) at Jianghan Fangcang shelter hospital. We assessed the prevalence of anxiety, depression symptoms and poor sleep quality via the scores of SAS, SDS and PSQI. We explored the influencing factors of anxiety and depression in COVID-19 patients using multivariable logistic regression models. Results We collected data from 307 COVID-19 patients in Jianghan Fangcang shelter hospital. The prevalence of anxiety, depression symptoms were 18.6% and 13.4%, respectively. Poor Sleep quality, number of current physical symptoms ≥ 2 were independent risk factors for anxiety symptoms ( P  < 0.05);female, family member confirmed COVID-19, number of current physical symptoms ≥ 2 were independent risk factors for depression symptoms ( P  < 0.05). PSQI scores were significant positively associate with SAS scores and SDS scores ( P ༜ 0.05). Conclusions Anxiety and depression are common among the COVID-19 patients in Fangcang shelter hospital. Those with more current physical symptoms, poor sleep quality are more likely to have anxiety. Females, those with their family members diagnosed with COVID-19, more current physical symptoms are more vulnerable to depression symptom. Our findings can be used to formulate targeted psychological interventions to reduce adverse psychological impacts in Fangcang shelter hospital during the outbreak of epidemic disease in the future.

14.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-325219

ABSTRACT

In the middle of March, the World Health Organization declared the outbreak of COVID-19 caused by SARS-CoV-2 infection a global pandemic. While China experienced a dramatic decline in daily growth rate of COVID-19, multiple importations of new cases from other countries and their related local infections caused a rapid rise. Between March 12 and April 15, we collected nasopharyngeal samples from 109 imported cases from 25 countries and 69 local cases in Guangzhou, China. In order to characterize the transmission patterns and genetic evolution of this virus among different populations, we sequenced the genome of SARS-CoV-2. The imported viral strains were assigned to lineages distributed in Europe (33.0%), America (17.4%), Africa (25.7%), or Southeast/West Asia (23.9%). Importantly, 10 imported cases from Africa formed two novel sub-lineages not identified in global tree previously. A detailed analysis showed that the imported viral strains from Philippines and Pakistan were closely related and within the same sub-lineage, whereas Ethiopia had varied lineages in the African phylogenetic tree. In spite of the diversity of imported SARS-CoV-2, 60 of 69 local infections could be traced back to two specific small lineages imported from Africa. A combined genetic and epidemiological analysis revealed a high-resolution transmission network of the imported SARS-CoV-2 in local communities, which might help inform the public health response and genomic surveillance in other cities and regions. Finally, we observed in-frame deletions on seven loci of SARS-CoV-2 genome, some of which were intra-host mutations, and they exhibited no enrichment on the S protein. Our findings provide new insight into the viral phylodynamics of SARS-CoV-2 and beta coronavirus.

15.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-324598

ABSTRACT

Due to the good clinical efficacy in treating Novel Coronavirus Pneumonia (NCP) resulted from SARS-CoV-2, as the traditional Chinese medicine(TCM) prescription, Lianhua Qingwen Formula (LQF) was composed into the Diagnosis and Treatment Programs of 2019 New Coronavirus Pneumonia (from fourth to seventh editions) formulated by the National Health Commission of China. Aiming to prevent and treat viral influenza, LQF was patented from 2003 in China, and passed the Phase II clinical trial by FDA in the United States in 2015. However, the molecular mechanism of LQF anti SARS-CoV-2 pneumonia is still not clear. It is shown that the docking scores of three components in LQF including Rutin, Forsythoside E, and Hyperoside to main protease of SARS-CoV-2 are very large as -9.1, -9.0 and -8.7 kcal/mol, respectively, which are even better than those of Lopinavir at -7.3 kcal/mol. Importantly, the binding modes between active compounds and protein were verified via molecular dynamics (MD) simulation and calculation all the binding free energies at MM-PBSA level. Note that these donor-acceptor systems were stabilized by non-polar interactions including hydrogen bonds and hydrophobic interactions. At last, from the constructed component-target-pathway network, it is shown that the components in LQF are related important pathways to improve the human immunity such as T cell, B cell receptor signaling, natural killer cell mediated cytotoxicity, as well as anti inflammatory pathways including Fc epsilon RI, ErbB, MAPK signaling and so on. The present investigation represents the first report on the molecular mechanism of LQF as NCP inhibitor

16.
EuropePMC; 2021.
Preprint in English | EuropePMC | ID: ppcovidwho-323703

ABSTRACT

Background: The coronavirus disease (COVID-19) pandemic has had catastrophic consequences globally. Nevertheless, the majority of the global population has not been vaccinated against the disease, and available treatments are limited. FeiDuQing (FDQ), a Chinese medicinal decoction widely used for COVID-19 pneumonia in Xianning City, China, has a cure rate of 98.21%. Therefore, evaluating the role of FDQ in successfully treating patients with COVID-19 is crucial. Methods In this retrospective cohort study, 355 consecutive patients who developed COVID-19 pneumonia between January 15 and February 18, 2020 were included;among them, 213 received FDQ. Data on the demographic characteristics, length of hospitalizations, symptoms at admission and discharge, adverse events, and laboratory parameters were analyzed. Results In contrast to patients who received FDQ, 12 patients who did not receive FDQ (8.45%) developed severe conditions, and one of them died. Furthermore, FDQ treatment was associated with a shortened duration of hospitalization (18.2 vs. 22.1 days, P  < 0.0001), even in elderly patients aged > 60 years (18.0 days vs. 26.1 days, P  < 0.0001). At discharge, three (1.40%) patients treated with FDQ had mild symptoms, whereas 16 (11.19%) patients not treated with FDQ had various symptoms. The cumulative survival rates of patients treated with FDQ and those not treated with FDQ were 79.04% and 32.60%, respectively (hazard ratio: 0.210, 95% confidence interval: 0.123–0.357, P  < 0.001). Additionally, FDQ had no severe adverse effects. Conclusions Our findings suggest that FDQ is a potential therapeutic candidate for fighting COVID-19.

17.
J Food Biochem ; 46(5): e14085, 2022 05.
Article in English | MEDLINE | ID: covidwho-1673175

ABSTRACT

SARS-CoV-2 wreaks havoc around the world, triggering the COVID-19 pandemic. It has been confirmed that the endoribonuclease NSP15 is crucial to the viral replication, and thus identified as a potential drug target against COVID-19. The NSP15 protein was used as the target to conduct high-throughput virtual screening on 30,926 natural products from the NPASS database to identify potential NSP15 inhibitors. And 100 ns molecular dynamics simulations were performed on the NSP15 and NSP15-NPC198199 system. In all, 10 natural products with high docking scores with NSP15 protein were obtained, among which compound NPC198199 scored the highest. The analysis of the binding mode between NPC198199 and NSP15 found that NPC198199 would form H-bond interactions with multiple key residues at the catalytic site. Subsequently, a series of post-dynamics simulation analyses (including RMSD, RMSF, PCA, DCCM, RIN, binding free energy, and H-bond occupancy) were performed to further explore inhibitory mechanism of compound NPC198199 on NSP15 protein at the molecular level. The research strongly indicates that the 10 natural compounds screened can be used as potential inhibitors of NSP15, and provides valuable information for the subsequent drug discovery of anti-SARS-CoV-2. PRACTICAL APPLICATIONS: Natural products play an important role in the treatment of many difficult diseases. In this study, high-throughput virtual screening technology was used to screen the natural product database to obtain potential inhibitors against endoribonuclease NSP15. The binding mechanism between natural products and NSP15 was investigated at the molecular level by molecular dynamics technology so that it is expected to become candidate drugs for the treatment of SARS-CoV-2. We hope that our research can provide new clue to combat COVID-19 and overcome the epidemic situation as soon as possible.


Subject(s)
Antiviral Agents , Biological Products , Endoribonucleases , SARS-CoV-2 , Viral Nonstructural Proteins , Antiviral Agents/chemistry , Antiviral Agents/pharmacology , Biological Products/pharmacology , COVID-19/drug therapy , Endoribonucleases/antagonists & inhibitors , Humans , Molecular Docking Simulation , Molecular Dynamics Simulation , SARS-CoV-2/drug effects , Viral Nonstructural Proteins/antagonists & inhibitors
18.
Br J Nutr ; 127(3): 398-402, 2022 02 14.
Article in English | MEDLINE | ID: covidwho-1665641

ABSTRACT

This commentary highlighted the background, take-home messages, and impacts of our 2007 British Journal of Nutrition paper entitled "Amino acids and immune function". In 2003-2004, there was an outbreak of severe acute respiratory syndrome (SARS) caused by SARS coronavirus-1 (CoV-1) in Asian countries. By the mid-2000's, clinical and experimental evidence indicated important roles for amino acids (AA) in improving innate and adaptive immunities in humans and animals. Based on our long-standing interest in AA metabolism and nutritional immunology, we decided to critically analyze advances in this nutritional field. Furthermore, we proposed a unified mechanism responsible for beneficial effects of AA and their products (including nitric oxide, glutathione, antibodies, and cytokines) on immune responses. We hoped that such integrated knowledge would be helpful for designing AA-based nutritional methods (e.g., supplementation with glutathione, arginine and glutamine) to prevent and treat SARS-like infectious diseases in the future. Our paper laid a framework for subsequent studies to quantify AA metabolism in intestinal bacteria, determine the effects of functional AA on cell-mediated and humoral immunities, and establish a much-needed database of AA composition in foodstuffs. Unexpectedly, COVID-19 (caused by SARS-CoV-2) emerged in December 2019 and has become one of the deadliest pandemics in history. Notably, glutathione, arginine and glutamine have now been exploited to effectively relieve severe respiratory symptoms of COVID-19 in affected patients. Functional AA (e.g., arginine, cysteine, glutamate, glutamine, glycine, taurine and tryptophan) and glutathione, which are all abundant in animal-sourced foodstuffs, are crucial for optimum immunity and health in humans and animals.


Subject(s)
Amino Acids , Immunity , Amino Acids/physiology , Animals , COVID-19 , Humans
19.
Int J Clin Pract ; 2022: 6498794, 2022.
Article in English | MEDLINE | ID: covidwho-1662352

ABSTRACT

Aim: To explore the potential relationship between NLR and micronutrient deficiency in patients with severe COVID-19 infection. Methods: Sixteen patients were categorized into the mild group (mild COVID-19) and severe group (severe COVID-19) based on the guideline of the management of COVID-19. The lactate dehydrogenase (LDH); superoxide dismutase (SOD), the inflammatory markers (neutrophil lymphocyte ratio (NLR)), erythrocyte sedimentation rate (ESR), c-reactive protein (CRP), selenium (Se), iron (Fe), zinc (Zn), nickel (Ni), copper (Cu), chromium (Cr), cadmium (Cd), arsenic (As), and manganese (Mn) were measured in the blood. Results: Compared to the mild group, the NLR (P < 0.05) and the level of Se (P < 0.01), Fe (P < 0.05), and Zn (P < 0.05) were significantly decreased in the severe group. The level of Se, Fe, and Zn was significantly correlated to NLR levels. Furthermore, close positive correlation was found between NLR and severity of COVID-19. Conclusion: The micronutrient deficiency in the blood is associated with NLR in the severity of COVID-19 patients.


Subject(s)
COVID-19 , Neutrophils , Humans , Lymphocytes , Micronutrients , Zinc
20.
Nano Res ; 15(4): 3323-3337, 2022.
Article in English | MEDLINE | ID: covidwho-1616263

ABSTRACT

The emergence of human coronaviruses (HCoVs), especially the current pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), engender severe threats to public health globally. Despite the outstanding breakthrough of new vaccines and therapeutic medicines in the past years, HCoVs still undergo unpredictable mutations, thus demanding more effective diagnostic and therapeutic strategies. Benefitting from the unique physicochemical properties and multiple nano-bio interactions, nanomaterials hold promising potential to fight against various HCoVs, either by providing sensitive and economic nanosensors for rapid viral detection, or by developing translatable nanovaccines and broad-spectrum nanomedicines for HCoV treatment. Herein, we systemically summarized the recent applications of nanoagents in diagnostics and therapeutics for HCoV-induced diseases, as well as their limitations and perspectives against HCoV variants. We believe this review will promote the design of innovative theranostic nanoagents for the current and future HCoV-caused pandemics.

SELECTION OF CITATIONS
SEARCH DETAIL