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1.
Chinese Journal of Biologicals ; 34(6):709-711, 2021.
Article in Chinese | EMBASE | ID: covidwho-1894339

ABSTRACT

Objective To prepare the indoor positive quality control reference for nucleic acid detection of 2019 Novel Corona Virus (2019-nCoV), investigate the feasibility of self-made quality control reference and evaluate the effect. Methods The positive control samples in the test kit from a third party were collected, diluted doubly with the virus preservation solution in which healthy human nasopharyngeal swabs were preserved, test samples to make a dilution, and determined for the Ct value of ORFlab gene by real-time fluorescent quantitative PCR. The dilutions with Ct values of about 31 and 34 were served as high-(nCov-H) and low-value quality control references (nCov-L) respectively and prepared in large quantities, then filled into small aliquots and stored at-80 °C to evaluate the uniformity and stability. Results The self-made indoor positive quality control reference for nucleic acid detection of 2019-nCoV showed good uniformity and good stability at various temperatures for storage, which could be kept stably at-80 °C for at least 6 months and could be controlled after repeated freezing and thawing for 3 times. Conclusion The self-made indoor positive quality control reference for nucleic acid detection of 2019-nCoV is simple to prepare, which has good uniformity and stability, and may be used as a substitute for commercial quality control for clinical test.

2.
Journal of Heart and Lung Transplantation ; 41(4):S131-S131, 2022.
Article in English | Web of Science | ID: covidwho-1849444
3.
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation ; 41(4):S131-S131, 2022.
Article in English | EuropePMC | ID: covidwho-1782282

ABSTRACT

Purpose Racial disparities in severe acute respiratory syndrome coronavirus 2 (COVID) incidence and mortality have been demonstrated in the United States (U.S.). Transplant recipients represent a particularly vulnerable population given their comorbidities and immunosuppression. With this in mind, we aimed to evaluate the relationship between race and mortality due to COVID in lung transplant recipients. Methods Adult lung transplant recipients in the U.S. were identified using the Organ Procurement and Transplantation (OPTN) database. Multiorgan transplants and patients transplanted after December 31, 2020 were excluded. Recipients who were deceased or lost to follow-up prior to January 2020 were excluded as they were not at risk for death due to COVID. Lung transplant recipients were stratified by race (Black, Hispanic, White, and other race). Death due to COVID was the primary outcome while all-cause mortality and non-COVID mortality were secondary outcomes. Student's t-test, Chi-square test, and Cox proportional hazards models were used for comparisons. Results 17,198 recipients met inclusion criteria (1,598 Black, 1,353 Hispanic, 13,755 White, and 492 other race). 231 (1.34%) deaths due to COVID were reported. COVID mortality rate was significantly different (p=0.001) by race, being lowest in White recipients (n=162 [1.18%]) and highest in Hispanic recipients (n=30 [2.22%]). Non-COVID mortality was lowest in Hispanic recipients (n=129 [9.53%]) and highest in Black recipients (n=236 [14.77%];p=0.008). There was no significant difference in all-cause mortality (p=0.054). After adjustment, Hispanic (HR=2.18;p=0.005) recipients experienced higher rates of mortality due to COVID compared to whites, but no significant difference in Black recipients (HR=1.73;p=0.066). See table 1 for additional predictors of death due to COVID. Conclusion Racial disparities in death due to COVID persist in U.S. lung transplant recipients, despite adjusting for social determinants of health.

4.
Zhongguo Huanjing Kexue/China Environmental Science ; 41(1):56-62, 2021.
Article in Chinese | Scopus | ID: covidwho-1130265

ABSTRACT

Based on the high-resolution TROPOMI data, this paper analyzed the spatial distribution of NO2 column density during the COVID-19 outbreaking period in China, and revealed the spatial variations in the NO2 changes during the year-on-year and month-on-month COVID-19 pandemic. The analysis showed that during the pandemic, the nationwide decline in NO2 column density compared to the NO2 level during the same period in 2019 (year-on-year) and during one-month before (month-on-month) the pandemic reached 40.46% and 50.09%, respectively. The economically developed and populous urban agglomerations had a significant decrease in emissions. Specifically, provinces with high historical NO2 emissions, including Jiangsu, Henan, Shandong, Zhejiang, etc., were more affected by the epidemic. Hubei showed the lowest NO2 column density (1.63×1015molec/cm2) during the epidemic period among the central and eastern provinces, with the NO2 decrease compared to the NO2 level during the same period in 2019 and during one-month before the pandemic greater than 50%. Regarding different cities in Hubei, the influence of the COVID-19 pandemic on the surrounding cities, e.g. Wuhan and Xiaogan, was much greater than that on the western mountainous cities, e.g. Shiyan and Enshi. The measurements of NO2 concentration at the ground-based national air quality monitoring stations also showed a consistent spatiotemporal trend with satellite observations, indicating that the "top-down" remote sensing method can efficiently reflects the intensity of air pollution emissions and the level of socio-economic activities in different regions. © 2021, Editorial Board of China Environmental Science. All right reserved.

7.
Chinese Traditional and Herbal Drugs ; 51(15):3937-3951, 2020.
Article in Chinese | EMBASE | ID: covidwho-769798

ABSTRACT

Objective: To explore the mechanism of Jingfang Granule in treatment of corona virus infection through biological information technology based on network pharmacology and molecular docking. Methods: The databases of TCMIP and TCMSP were used to summarize the flavor and meridian tropism and active compounds of Jingfang Granule, and the potential targets of active compounds were searched by PubChem and SwissTargetPrediction. The corona virus targets were collected from the GeneCards database. And common targets were enriched and analyzed by DAVID database after the intersection of the compounds targets and the disease targets. Then the network of "TCM-Ingredients-Common targets" was established by Cytoscape 3.7.2, the main active components and key targets were screened for molecular docking. Results: Totally 139 active components of Jingfang Granule and 27 common targets were obtained. GO enrichment analysis and KEGG enrichment analysis found that the pathways in cancer, MAPK signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway were the main pathways of Jingfang Granule in treatment of corona virus infection. The network of "TCM-Ingredients-Common targets" was successfully constructed, and results of molecular docking showed that the main components in this network such as β-sitosterol, cerevisterol, isorhamnetin, hesperetin, and luteolin etc., have good affinity with key targets of VEGFA, IL6, TNF, PPARγ, APP, ACE2, and SARS-CoV-2 3CL hydrolase. Conclusion: Jingfang Granule treats corona virus infectious diseases through the compatibility of multiple traditional Chinese medicine. Its resistance to corona virus infection may be through the β-sitosterol, cerevisterol, isorhamnetin, hesperetin, and luteolin act on the VEGFA, IL6, TNF, PPARγ, APP and other targets, and then affects the pathways in cancer, MAPK signal pathway, PI3K-Akt signal pathway, TNF signal pathways to achieve.

8.
Zhonghua Xin Xue Guan Bing Za Zhi ; 48(6): 456-460, 2020 Jun 24.
Article in Chinese | MEDLINE | ID: covidwho-8400

ABSTRACT

Objective: To analyze the clinical characteristics of the severe or critically ill patients with novel coronavirus pneumonia (COVID-19), and evaluate the impact of complicated myocardial injury on the prognosis of these patients. Methods: A retrospective study was conducted in 54 patients who admitted to Tongji hospital from February 3, 2020 to February 24, 2020 and met the criteria of severe or critical conditions of COVID-19. The clinical characteristics and hospital mortality rate were analyzed and compared between the patients with or without myocardial injury, which was defined with 3 times higher serum cardiac troponin value. Results: The age of the 54 patients was 68.0(59.8, 74.3) years. Among all the patients, 24 (44.4%) patients were complicated with hypertension, 13 (24.1%) with diabetes, 8 (14.8%) with coronary heart disease, and 3 (5.6%) with previous cerebral infarction. During hospitalization, 24 (44.4%) of the patients were complicated with myocardial injury and 26 (48.1%) patients died in hospital. In-hospital mortality was significantly higher in patients with myocardial injury than in patients without myocardial injury (14 (60.9%) vs. 8 (25.8%), P=0.013). Moreover, the levels of C-reactive protein (153.6 (80.3, 240.7) ng/L vs. 49.8 (15.9, 101.9) ng/L) and N-terminal pro-B-type natriuretic peptide (852.0 (400.0, 2 315.3) ng/L vs. 197.0 (115.3, 631.0) ng/L) were significantly higher than patients without myocardial injury (all P<0.01). Conclusions: Prevalence of myocardial injury is high among severe or critically ill COVID-19 patients. Severe or critically ill COVID-19 patients with myocardial injury face a significantly higher risk of in-hospital mortality. The study suggests that it is important to monitor and manage the myocardial injury during hospitalization for severe or critically ill COVID-19 patients.


Subject(s)
Betacoronavirus , Coronavirus Infections , Critical Illness , Heart Injuries , Pandemics , Pneumonia, Viral , Aged , COVID-19 , Coronavirus Infections/complications , Humans , Middle Aged , Pneumonia, Viral/complications , Retrospective Studies , SARS-CoV-2
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