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Cytokine ; 143: 155523, 2021 07.
Article in English | MEDLINE | ID: covidwho-1163610


Cytokines play pleiotropic, antagonistic, and collaborative in viral disease. The high morbidity and mortality of coronavirus disease 2019 (COVID-19) make it a significant threat to global public health. Elucidating its pathogenesis is essential to finding effective therapy. A retrospective study was conducted on 71 patients hospitalized with COVID-19. Data on cytokines, T lymphocytes, and other clinical and laboratory characteristics were collected from patients with variable disease severity. The effects of cytokines on the overall survival (OS) and event-free survival (EFS) of patients were analyzed. The critically severe and severe patients had higher infection indexes and significant multiple organ function abnormalities than the mild patients (P < 0.05). IL-6 and IL-10 were significantly higher in the critically severe patients than in the severe and mild patients (P < 0.05). IL-6 and IL-10 were closely associated with white blood cells, neutrophils, T lymphocyte subsets, D-D dimer, blood urea nitrogen, complement C1q, procalcitonin C-reactive protein. Moreover, the IL-6 and IL-10 levels were closely correlated to dyspnea and dizziness (P < 0.05). The patients with higher IL-10 levels had shorter OS than the group with lower levels (P < 0.05). The older patients with higher levels of single IL-6 or IL-10 tended to have shorter EFS (P < 0.05), while the patients who had more elevated IL-6 and IL-10 had shorter OS (P < 0.05). The Cox proportional hazard model revealed that IL-6 was the independent factor affecting EFS. IL-6 and IL-10 play crucial roles in COVID-19 prognosis.

COVID-19/blood , COVID-19/pathology , Interleukin-10/blood , Interleukin-6/blood , T-Lymphocyte Subsets/immunology , Adult , Age Factors , Aged , Aging , Blood Coagulation Factors/analysis , COVID-19/mortality , COVID-19/therapy , Cytokine Release Syndrome/pathology , Female , Humans , Lymphocyte Count , Male , Middle Aged , Retrospective Studies , SARS-CoV-2/immunology , Severity of Illness Index , Survival Analysis , T-Lymphocyte Subsets/cytology , Thromboembolism/pathology , Treatment Outcome
Med. J. Wuhan Univ. ; 4(41): 517-520, 20200715.
Article in Chinese | WHO COVID, ELSEVIER | ID: covidwho-617504


Objective: To compare the chemiluminescence and colloidal gold method in the detection of 2019 novel Coronavirus (2019-nCoV, or SARS-CoV-2) specific antibody and to evaluate the importance in the diagnosis and treatment of COVID-19. Methods: A total of 191 cases of diagnosed and suspected COVID-19 patients admitted in Febrary 2020 in Wuhan Leishenshan Hospital were included. The patients were divided into 2019-nCoV continuous positive group, 2019-nCoV positive to negative group, and 2019-nCoV suspect group according to the results of pharyngeal swab 2019-nCoV nucleic acid detection. In addition, 27 patients without COVID-19 were set as control group to test the specificity. The serum samples were collected, and colloidal gold and chemiluminescence method were used to detect total antibodies (IgM+IgG) and IgM. SPSS 24.0 was used to compare the difference in antibody detection rates between the two methods. Results: The specificity of total antibodies (IgM+IgG) and IgM detection was 100%. Two methods of total antibody detection had no significant difference in continuous positive group (P=1.00). In positive to negative group and suspect group, the positive detection rate of chemiluminescence method was significantly higher than colloidal gold method (95.2% vs 76.2% and 94.9% vs 70.5%, respectively, both P<0.05). The chemiluminescence method showed no significant difference between the three groups in IgM detection (P>0.05). Conclusion: The detection of 2019-nCoV specific antibody can be an important supplemental method to pharyngeal swab 2019-nCoV nucleic acid detection during the diagnosis and treatment of COVID-19. The chemiluminescence method is better than colloidal gold method in the detection of total antibody. The IgM detection using chemiluminescence method is suitable for all stages of the disease.

J Infect ; 81(1): e28-e32, 2020 07.
Article in English | MEDLINE | ID: covidwho-46420


An outbreak of new coronavirus SARS-CoV-2 was occurred in Wuhan, China and rapidly spread to other cities and nations. The standard diagnostic approach that widely adopted in the clinic is nucleic acid detection by real-time RT-PCR. However, the false-negative rate of the technique is unneglectable and serological methods are urgently warranted. Here, we presented the colloidal gold-based immunochromatographic (ICG) strip targeting viral IgM or IgG antibody and compared it with real-time RT-PCR. The sensitivity of ICG assay with IgM and IgG combinatorial detection in nucleic acid confirmed cases were 11.1%, 92.9% and 96.8% at the early stage (1-7 days after onset), intermediate stage (8-14 days after onset), and late stage (more than 15 days), respectively. The ICG detection capacity in nucleic acid-negative suspected cases was 43.6%. In addition, the concordance of whole blood samples and plasma showed Cohen's kappa value of 0.93, which represented the almost perfect agreement between two types of samples. In conclusion, serological ICG strip assay in detecting SARS-CoV-2 infection is both sensitive and consistent, which is considered as an excellent supplementary approach in clinical application.

Antibodies, Viral/blood , Betacoronavirus/immunology , Coronavirus Infections/blood , Coronavirus Infections/virology , Immunoassay/methods , Pneumonia, Viral/blood , Pneumonia, Viral/virology , Serologic Tests , Adult , Aged , Aged, 80 and over , COVID-19 , Coronavirus Infections/diagnosis , Female , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Male , Middle Aged , Pandemics , Pneumonia, Viral/diagnosis , Real-Time Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction , SARS-CoV-2 , Young Adult