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1.
J Am Chem Soc ; 144(20): 9057-9065, 2022 05 25.
Article in English | MEDLINE | ID: covidwho-1839492

ABSTRACT

Glycosylation of proteins is a complicated post-translational modification. Despite the significant progress in glycoproteomics, accurate functions of glycoproteins are still ambiguous owing to the difficulty in obtaining homogeneous glycopeptides or glycoproteins. Here, we describe a streamlined chemoenzymatic method to prepare complex glycopeptides by integrating hydrophobic tag-supported chemical synthesis and enzymatic glycosylations. The hydrophobic tag is utilized to facilitate peptide chain elongation in the liquid phase and expeditious product separation. After removal of the tag, a series of glycans are installed on the peptides via efficient glycosyltransferase-catalyzed reactions. The general applicability and robustness of this approach are exemplified by efficient preparation of 16 well-defined SARS-CoV-2 O-glycopeptides, 4 complex MUC1 glycopeptides, and a 31-mer glycosylated glucagon-like peptide-1. Our developed approach will open up a new range of easy access to various complex glycopeptides of biological importance.


Subject(s)
COVID-19 , Glycopeptides , SARS-CoV-2 , Glycopeptides/chemical synthesis , Glycopeptides/chemistry , Glycoproteins/chemistry , Glycosylation , Humans , Peptides/metabolism , SARS-CoV-2/chemistry
2.
SciFinder; 2020.
Preprint | SciFinder | ID: ppcovidwho-4434

ABSTRACT

A review. Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) has caused serious health threat in the society. In addition to minimize the spread of the virus, it is in urgent need to explore new treatment strategies, develop new treatment drugs to improve patient survival and quality of life. This article reviews current approaches to develop antiviral drugs. In addition, based on the genomic characteristics of the novel coronavirus and the research advances of RNA interference (RNAi) technique in the related fields, we analyze the feasibility, advantages and problems to overcome of RNA interference techniques in prevention of COVID-19 infection and treatment of patients.

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