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1.
J Med Virol ; 94(4): 1693-1695, 2022 Apr.
Article in English | MEDLINE | ID: covidwho-1718388

ABSTRACT

SARS-CoV-2 rapid detection is of great interest to prevent viral dissemination. In that sense, antigen tests appeared as a very valuable tool to reach this goal. However, it is possible to obtain a negative result in those patients with low viral loads, and consequently, reverse transcription-polymerase chain reaction (RT-PCR) should be performed on samples from patients with a negative antigen test in which there is a strong suspicion of COVID infection. The common diagnostic algorithm involves taking a second sample for RT-PCR testing. This study evaluates the usefulness of the antigen test sample for carrying out RT-PCR analysis when necessary. Results obtained indicate that can be used a unique sample for both antigen test and RT-PCR. This data showed that it is possible to reduce excessive suspected individuals managing and so on increase staff security and patient comfort.


Subject(s)
COVID-19 Nucleic Acid Testing , COVID-19 Serological Testing , SARS-CoV-2/isolation & purification , COVID-19/diagnosis , COVID-19/virology , Humans , SARS-CoV-2/genetics , SARS-CoV-2/immunology , Sensitivity and Specificity , Specimen Handling , Viral Load
2.
J Med Virol ; 94(4): 1693-1695, 2022 Apr.
Article in English | MEDLINE | ID: covidwho-1520242

ABSTRACT

SARS-CoV-2 rapid detection is of great interest to prevent viral dissemination. In that sense, antigen tests appeared as a very valuable tool to reach this goal. However, it is possible to obtain a negative result in those patients with low viral loads, and consequently, reverse transcription-polymerase chain reaction (RT-PCR) should be performed on samples from patients with a negative antigen test in which there is a strong suspicion of COVID infection. The common diagnostic algorithm involves taking a second sample for RT-PCR testing. This study evaluates the usefulness of the antigen test sample for carrying out RT-PCR analysis when necessary. Results obtained indicate that can be used a unique sample for both antigen test and RT-PCR. This data showed that it is possible to reduce excessive suspected individuals managing and so on increase staff security and patient comfort.


Subject(s)
COVID-19 Nucleic Acid Testing , COVID-19 Serological Testing , SARS-CoV-2/isolation & purification , COVID-19/diagnosis , COVID-19/virology , Humans , SARS-CoV-2/genetics , SARS-CoV-2/immunology , Sensitivity and Specificity , Specimen Handling , Viral Load
3.
J Pediatr ; 232: 287-289.e4, 2021 05.
Article in English | MEDLINE | ID: covidwho-1126937

ABSTRACT

We conducted a multicenter clinical validity study of the Panbio coronavirus disease 2019 Antigen Rapid Test of nasopharyngeal samples in pediatric patients with coronavirus disease 2019-compatible symptoms of ≤5 days of evolution. Our study showed limited accuracy in nasopharyngeal antigen testing: overall sensitivity was 45.4%, and 99.8% of specificity, positive-predictive value was 92.5%.


Subject(s)
Antigens, Viral/analysis , COVID-19/diagnosis , DNA, Viral/analysis , Nasopharynx/virology , Reverse Transcriptase Polymerase Chain Reaction/methods , SARS-CoV-2/genetics , Adolescent , COVID-19/virology , Child , Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Pandemics , Reproducibility of Results , SARS-CoV-2/immunology
4.
Clin Microbiol Infect ; 2021 Feb 16.
Article in English | MEDLINE | ID: covidwho-1085573

ABSTRACT

OBJECTIVES: The standard RT-PCR assay for coronavirus disease 2019 (COVID-19) is laborious and time-consuming, limiting testing availability. Rapid antigen-detection tests are faster and less expensive; however, the reliability of these tests must be validated before they can be used widely. The objective of this study was to determine the performance of the Panbio™ COVID-19 Ag Rapid Test Device (PanbioRT) (Abbott) in detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in nasopharyngeal swab specimens. METHODS: This prospective multicentre study was carried out in ten Spanish university hospitals and included individuals with clinical symptoms or epidemiological criteria of COVID-19. Only individuals with ≤7 days from the onset of symptoms or from exposure to a confirmed case of COVID-19 were included. Two nasopharyngeal samples were taken to perform the PanbioRT as a point-of-care test and a diagnostic RT-PCR test. RESULTS: Among the 958 patients studied, 325 (90.5%) had true-positive results. The overall sensitivity and specificity for the PanbioRT were 90.5% (95%CI 87.5-93.6) and 98.8% (95%CI 98-99.7), respectively. Sensitivity in participants who had a threshold cycle (CT) < 25 for the RT-PCR test was 99.5% (95%CI 98.4-100), and in participants with ≤5 days of the clinical course it was 91.8% (95%CI 88.8-94.8). Agreement between techniques was 95.7% (κ score 0.90; 95%CI 0.88-0.93). CONCLUSIONS: The PanbioRT performs well clinically, with even more reliable results for patients with a shorter clinical course of the disease or a higher viral load. The results must be interpreted based on the local epidemiological context.

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