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1.
Int J Mol Sci ; 23(4)2022 Feb 14.
Article in English | MEDLINE | ID: covidwho-1686821

ABSTRACT

The ongoing COVID-19 pandemic dictated new priorities in biomedicine research. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is a single-stranded positive-sense RNA virus. In this pilot study, we optimized our padlock assay to visualize genomic and subgenomic regions using formalin-fixed paraffin-embedded placental samples obtained from a confirmed case of COVID-19. SARS-CoV-2 RNA was localized in trophoblastic cells. We also checked the presence of the virion by immunolocalization of its glycoprotein spike. In addition, we imaged mitochondria of placental villi keeping in mind that the mitochondrion has been suggested as a potential residence of the SARS-CoV-2 genome. We observed a substantial overlapping of SARS-CoV-2 RNA and mitochondria in trophoblastic cells. This intriguing linkage correlated with an aberrant mitochondrial network. Overall, to the best of our knowledge, this is the first study that provides evidence of colocalization of the SARS-CoV-2 genome and mitochondria in SARS-CoV-2 infected tissue. These findings also support the notion that SARS-CoV-2 infection can reprogram mitochondrial activity in the highly specialized maternal-fetal interface.


Subject(s)
Mitochondria/virology , Nucleic Acid Amplification Techniques/methods , Placenta/virology , RNA, Viral/metabolism , SARS-CoV-2/genetics , Adult , COVID-19/pathology , COVID-19/virology , DNA Probes/metabolism , Female , Humans , Pilot Projects , Placenta/pathology , Pregnancy , SARS-CoV-2/isolation & purification
2.
Sci Prog ; 104(2): 36850421998487, 2021.
Article in English | MEDLINE | ID: covidwho-1207543

ABSTRACT

The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) pandemic had a significant impact on the Italian healthcare system, although geographical differences were present; regions in northern Italy have been the most severely affected while regions in the south of the country were relatively spared. Otolaryngologists were actively involved in the management of the pandemic. In this work, we analyzed and compared the otolaryngology surgical activity performed during the pandemic in two large public hospitals located in different Italian regions. In northern Italy, otolaryngologists were mainly involved in performing surgical tracheotomies in COVID-19 positive patients and contributed to the management of these patients in intensive care units. In central Italy, where the burden of the infection was significantly lower, otolaryngologists focused on diagnosis and treatment of emergency and oncology patients. This analysis confirms the important role of the otolaryngology specialists during the pandemic, but also highlights specific differences between two large hospitals in different Italian regions.


Subject(s)
COVID-19/epidemiology , Medical Oncology/organization & administration , Otolaryngology/organization & administration , Pandemics , SARS-CoV-2/pathogenicity , COVID-19/pathology , COVID-19/surgery , COVID-19/virology , Geography , Hospitals , Humans , Intensive Care Units , Italy/epidemiology , Patient Admission/statistics & numerical data , Surveys and Questionnaires , Tracheotomy
3.
Bosn J Basic Med Sci ; 21(1): 117-119, 2021 Feb 01.
Article in English | MEDLINE | ID: covidwho-1052562

ABSTRACT

Since the COVID-19 outbreak, Italy has been one of the most affected countries in Europe and the second for number of deaths. In this commentary, we discuss some lessons that we learned as health-care providers working in a large public hospital during the pandemic, with a special focus on the importance of infection containment and early diagnosis, the role of swab, serological tests, home isolation and individual protection  devices, and the available therapies and management indications to better face a possible new outbreak in the near future. These comments should stimulate a more diffused, efficient, and efficacious management of COVID-19 patients, also reducing the number of admissions to hospital emergency departments and the related spread of the infection.


Subject(s)
COVID-19 Nucleic Acid Testing , COVID-19 Serological Testing , COVID-19/epidemiology , COVID-19/therapy , Communicable Disease Control , Critical Care , Health Personnel , Hospitalization , Hospitals, Public , Humans , Italy/epidemiology , Pandemics , Patient Admission , Physical Distancing , Quarantine , Treatment Outcome
4.
Otolaryngol Head Neck Surg ; 163(1): 86-88, 2020 07.
Article in English | MEDLINE | ID: covidwho-306370

ABSTRACT

Otolaryngology and head and neck surgery underwent drastic changes during the COVID-19 pandemic. Since March 10, the first day of the lockdown in Italy, diagnostic and therapeutic procedures were limited to emergency and oncology cases, while outpatient procedures and clinical examinations were temporarily suspended to limit virus diffusion and to reallocate personnel into wards dedicated to COVID-19. In our otolaryngology unit, between March 10 and April 28, 2020, we performed 96 surgical procedures; they mainly consisted in diagnosis and treatment of malignant tumors of the head and neck (77%), management of acute upper airway obstruction in adults and children (14.7%), drainage of abscesses of the head and neck (6.2%), and treatment of nasal bone fractures (2.1%). When comparing these data with those of the same period in 2019 for emergency and oncology procedures, we noticed a drastic reduction of head and neck abscesses and nasal bone fractures, while oncology surgery remained stable.


Subject(s)
Betacoronavirus , Coronavirus Infections/epidemiology , Disease Transmission, Infectious/prevention & control , Head and Neck Neoplasms/surgery , Hospitals, University , Otolaryngology/organization & administration , Otorhinolaryngologic Surgical Procedures/trends , Pneumonia, Viral/epidemiology , COVID-19 , Coronavirus Infections/complications , Coronavirus Infections/transmission , Head and Neck Neoplasms/complications , Humans , Italy , Pandemics , Pneumonia, Viral/complications , Pneumonia, Viral/transmission , SARS-CoV-2
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