Your browser doesn't support javascript.
Show: 20 | 50 | 100
Results 1 - 5 de 5
Filter
1.
Rev Gaucha Enferm ; 43: e20210038, 2022.
Article in English, Portuguese | MEDLINE | ID: covidwho-1974443

ABSTRACT

OBJECTIVE: To build a theoretical model that represents the experience of family caregivers of elderly people during the COVID-19 pandemic. METHOD: Qualitative study that used the Grounded Theory as a methodology, carried out in Minas Gerais State in August 2020. 16 caregivers were interviewed. RESULTS: Uncomfortable emotions emerged and drove the caregivers' actions to: adopt preventive measures to protect the elderly from contracting the coronavirus; guarantee their healthcare; and make them aware of the pandemic. However, when these emotions manifested in an exacerbated manner, they needed to be alleviated so that they could adopt such initiatives. CONCLUSION: The emotions felt by caregivers can compromise their health and quality of life. Therefore, health professionals need to create strategies to ensure that they are well assisted, enabling care through telehealth. Guidance on the pandemic for caregivers could reflect better care for the elderly people.


Subject(s)
COVID-19 , Caregivers , Aged , COVID-19/epidemiology , Caregivers/psychology , Family/psychology , Humans , Pandemics , Qualitative Research , Quality of Life
2.
Nat Biotechnol ; 2022 Jul 21.
Article in English | MEDLINE | ID: covidwho-1947381

ABSTRACT

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with potential resistance to existing drugs emphasizes the need for new therapeutic modalities with broad variant activity. Here we show that ensovibep, a trispecific DARPin (designed ankyrin repeat protein) clinical candidate, can engage the three units of the spike protein trimer of SARS-CoV-2 and inhibit ACE2 binding with high potency, as revealed by cryo-electron microscopy analysis. The cooperative binding together with the complementarity of the three DARPin modules enable ensovibep to inhibit frequent SARS-CoV-2 variants, including Omicron sublineages BA.1 and BA.2. In Roborovski dwarf hamsters infected with SARS-CoV-2, ensovibep reduced fatality similarly to a standard-of-care monoclonal antibody (mAb) cocktail. When used as a single agent in viral passaging experiments in vitro, ensovibep reduced the emergence of escape mutations in a similar fashion to the same mAb cocktail. These results support further clinical evaluation of ensovibep as a broad variant alternative to existing targeted therapies for Coronavirus Disease 2019 (COVID-19).

3.
J Virol ; 96(14): e0065322, 2022 07 27.
Article in English | MEDLINE | ID: covidwho-1938007

ABSTRACT

Infectious bronchitis virus (IBV) is an avian coronavirus that causes infectious bronchitis, an acute and highly contagious respiratory disease of chickens. IBV evolution under the pressure of comprehensive and widespread vaccination requires surveillance for vaccine resistance, as well as periodic vaccine updates. Reverse genetics systems are very valuable tools in virology, as they facilitate rapid genetic manipulation of viral genomes, thereby advancing basic and applied research. We report here the construction of an infectious clone of IBV strain Beaudette as a bacterial artificial chromosome (BAC). The engineered full-length IBV clone allowed the rescue of an infectious virus that was phenotypically indistinguishable from the parental virus. We used the infectious IBV clone and examined whether an enhanced green fluorescent protein (EGFP) can be produced by the replicase gene ORF1 and autocatalytically released from the replicase polyprotein through cleavage by the main coronavirus protease. We show that IBV tolerates insertion of the EGFP ORF at the 3' end of the replicase gene, between the sequences encoding nsp13 and nsp16 (helicase, RNA exonuclease, RNA endonuclease, and RNA methyltransferase). We further show that EGFP is efficiently cleaved from the replicase polyprotein and can be localized in double-membrane vesicles along with viral RNA polymerase and double-stranded RNA, an intermediate of IBV genome replication. One of the engineered reporter EGFP viruses were genetically stable during passage in cultured cells. We demonstrate that the reporter EGFP viruses can be used to study virus replication in host cells and for antiviral drug discovery and development of diagnostic assays. IMPORTANCE Reverse genetics systems based on bacterial artificial chromosomes (BACs) are the most valuable systems in coronavirus research. Here, we describe the establishment of a reverse genetics system for the avian coronavirus strain Beaudette, the most intensively studied strain. We cloned a copy of the avian coronavirus genome into a BAC vector and recovered infectious virus in permissive cells. We used the new system to construct reporter viruses that produce enhanced green fluorescent protein (EGFP). The EGFP coding sequence was inserted into 11 known cleavage sites of the major coronavirus protease in the replicase gene ORF1. Avian coronavirus tolerated the insertion of the EGFP coding sequence at three sites. The engineered reporter viruses replicated with parental efficiency in cultured cells and were sufficiently genetically stable. The new system facilitates functional genomics of the avian coronavirus genome but can also be used for the development of novel vaccines and anticoronaviral drugs.


Subject(s)
Coronavirus Infections , Infectious bronchitis virus , Reverse Genetics , Animals , Chickens , Coronavirus Infections/veterinary , Genes, Reporter , Green Fluorescent Proteins , Infectious bronchitis virus/genetics , Peptide Hydrolases , Polyproteins , RNA, Viral/genetics
4.
EuropePMC;
Preprint in Portuguese | EuropePMC | ID: ppcovidwho-328154

ABSTRACT

Devido à pandemia do SARS-CoV-2, a Universidade Federal de Minas Gerais (UFMG) estabeleceu o Ensino Remoto Emergencial (ERE), em julho de 2020, com o objetivo de dar continuidade ao primeiro semestre letivo de aulas na instituição. Considerando o período adaptativo, objetivou-se avaliar a satisfação dos discentes do curso de Farmácia com o ERE, focando no processo de aprendizagem. Realizou-se coleta de dados por meio de plataforma virtual, com questionário sobre dados sociodemográficos, hábitos de vida e processo de ensino-aprendizagem. Os dados foram analisados por meio de regressão linear simples e da técnica CART. O perfil dos alunos respondentes foi caracterizado majoritariamente por mulheres e estudantes do ciclo profissionalizante da graduação (acima do 5º período). A média de satisfação geral com o ensino dos discentes foi de 3,12 (em uma escala que variou de 1 a 5), apresentando correlação inversa ao aprendizado no ciclo profissionalizante do curso (p<0,000). Apenas 3,1% dos alunos que indicaram “nenhum ou pouco aprendizado” atribuíram satisfação máxima com o ERE. Observou-se menor satisfação com a qualidade das aulas práticas (3,06), revelando a limitação do ERE para o desenvolvimento de cursos com conteúdos tão práticos como o de Farmácia. Por fim, é retratada uma satisfação acima da média para fatores relacionados à didática e metodologias dos docentes, mostrando o compromisso e dedicação dos professores ao ERE, apesar das dificuldades para o seu estabelecimento.

5.
Revista de Ciencias Farmaceuticas Basica e Aplicada ; 40:3-3, 2019.
Article in English | LILACS (Americas) | ID: covidwho-1016816

ABSTRACT

The use of chloroquine and hydroxychloroquine as off-label treatments for covid-19 disease is a resort for critical care patients under enteral nutrition (EN). However, the use of solid pharmaceutical forms of these drugs through feeding tubes can pose a challenge to the health care team. Therefore, we performed a review of literature regarding administration of chloroquine and hydroxychloroquine through feeding tubes. For this end, a search was performed on PubMed and Lilacs database using key-words and free terms referring to drug administration via feeding tubes, and, specifically chloroquine and hydroxychloroquine. Also, a search on Micromedex® database and on the Handbook of Drug Administration via Enteral Feeding Tubes were performed. A total of 1.784 articles were retrieved. However, 4 articles fitted in the inclusion criteria. Two articles exploring the administration of chloroquine via feeding tubes on children with malaria found no difference on clinical results or tolerability when comparing it with oral or intramuscular administration. Other article showed full dispersion of hydroxychloroquine on water after crushing with mortar and pestle. A review found no information regarding the administration of hydroxychloroquine via postpyloric feeding tubes. No information was found on Micromedex® or the consulted Handbook;however, they pointed out the interaction between chloroquine and multivalent ions if coadministered.

SELECTION OF CITATIONS
SEARCH DETAIL