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Bioinformation ; 18(12):1154-1158, 2022.
Article in English | Web of Science | ID: covidwho-2308405
European Journal of Molecular and Clinical Medicine ; 7(9):3630-3638, 2020.
Article in English | EMBASE | ID: covidwho-2276897
Coronaviruses ; 2(4):507-515, 2021.
Article in English | EMBASE | ID: covidwho-2273894
International Journal of Software Innovation ; 11(1):27-27, 2023.
Article in English | Web of Science | ID: covidwho-2234694


Social distancing has been imposed to prevent substantial transmission of the COVID-19 outbreak, which is presently a global public health issue. Medical healthcare providers rely on telemedicine to monitor their patients, particularly those with chronic conditions. However, telemedicine faces many implementation-related risks, including data breaches, access restrictions within the medical community, inaccurate diagnosis, fraud, etc. The authors propose a transparent, tamper-proof, distributed, decentralized smart healthcare system (DSHS) that uses blockchain-based smart contracts. The authors use an immutable modified Merkel tree structure to hold the transaction for viewing contracts on a public blockchain, updating patient health records (PHR), and exchanging PHR to all entities. It is verified by a performance evaluation based on the Ethereum platform. The simulation results show that the proposed system outperforms existing approaches by enhancing transparency, boosting efficiency, and reducing average latency in the system. The proposed system improves the functionality of the SHS environment.

American Journal of the Medical Sciences ; 365(Supplement 1):S388-S389, 2023.
Article in English | EMBASE | ID: covidwho-2227303


Case Report: Acute motor and sensory axonal neuropathy (AMSAN) syndrome is a rare subtype of Guillain-Barre syndrome (GBS) with poor recovery [1]. While respiratory and gastrointestinal infections may precipitate AMSAN, an underlying autoimmune disorder is seldom reported in literature. We herein report the complex case of a patient with undiagnosed, asymptomatic mixed connective tissue disease (MCTD) who developed AMSAN syndrome. Case: A 44-year-old Asian male without medical history presented with progressively worsening weakness of both upper and lower extremities and inability to perform daily activities. His symptoms started 12 weeks prior with difficulty standing from a seated position. He felt subjectively better for some time until a week prior, when he became bedbound. He had diarrhea 6 months ago, with 5-6 loose bowel movements a day for a few weeks. Vital signs on admission was normal. On neurological examination, he was alert and oriented, with bilateral upper and lower extremity flaccid paralysis, diffuse muscle atrophy, bilateral hand and foot drop, negative Hoover sign, diffuse areflexia, and intact sensation. Cerebrospinal fluid (CSF) analysis showed WBC 0 and protein level 136. MRI cervical, thoracic, and lumbar spine were normal. EMG revealed sensory involvement with positive sharp waves in proximal muscles along with fibrillations. Intravenous immunoglobulin (IVIG) was initiated at 0.4 mg/kg for 5 days. Infectious workup for COVID-19, stool culture, HIV, TB, RPR and campylobacter jejuni antibody (Ab), was negative. ANA was positive in a speckled pattern with titres 1:1280, with a positive RNP Ab, SS-A, and RF IgM, IgG and IgA. Rest of the autoimmune workup (anti-dsDNA, anti-CCP, SS-B, aldolase, anti-Jo-1, anti-Scl-70, p-ANCA, c-ANCA, anti-GM1, anti-GQ1b, and anti-GD1a ganglioside Ab) was negative. The myositis specific 11 Ab panel was negative. Despite IVIG therapy, he developed dysphagia, respiratory distress, with a negative inspiratory force of -0, requiring intubation. He had a tracheostomy and PEG tube placed and remains quadraplegic nearly 120 days later. Discussion(s): The authors report a unique case of a patient who became progressively weak over 3 months, leading to complete quadriplegia. Interestingly, this is more consistent with chronic inflammatory demyelinating poly-neuropathy (CIDP), as AMSAN typically develops over a short period of 2 to 4 weeks [2]. Despite having negative anti-GM1 and anti-GD1a Ab (in which positive Ab are pathognomonic but not always present in AMSAN syndrome), the patient had weakness that began in the lower extremities, progressing to paralysis, along with albuminocytological dissociation on CSF analysis, pointing to a GBS diagnosis [3]. He had sensory involvement in the EMG, thus making the diagnosis as AMSAN. He had an undiagnosed, asymptomatic autoimmune process most consistent with MCTD. Whether the two disease processes are related to each other is a concept that has not yet been investigated. Pediatric Clinical Case Reports Concurrent Session Saturday February 4, 2023 1:00 PM Copyright © 2023 Southern Society for Clinical Investigation.

9th IEEE Uttar Pradesh Section International Conference on Electrical, Electronics and Computer Engineering, UPCON 2022 ; 2022.
Article in English | Scopus | ID: covidwho-2213396
The American Journal of the Medical Sciences ; 365:S119, 2023.
Article in English | ScienceDirect | ID: covidwho-2211704
European Journal of Molecular and Clinical Medicine ; 10(1):3014-3038, 2023.
Article in English | EMBASE | ID: covidwho-2207374
International Journal of Software Innovation ; 11(1), 2022.
Article in English | Scopus | ID: covidwho-2201331
MethodsX ; 10: 102011, 2023.
Article in English | MEDLINE | ID: covidwho-2181610


SARS-CoV-2 is a public pandemic health concern globally. Nasopharyngeal and oropharyngeal swab samples are used for Covid-19 viral detection. Sample collection procedure was tedious and uncomfortable and unsuitable for biochemical and CBC analysis in swab samples. Biochemistry and CBC tests are key determinant in management of Covid-19 patients. We developed a LAMP test to detect viral RNA in blood samples. LAMP is required four specific primers targeting the internal transcribed S-region and loop primers for viral RNA amplification. RNA was extracted from blood samples by TRIzol method. LAMP reaction was performed at 60 °C for 1 hour and amplicons were visualized in HNB dye. No cross-reactivity was seen with HBV, HCV, and HIV infected sample. Out of 40 blood samples, 33 samples were positive for LAMP and Q-PCR analysis, one sample was positive for LAMP and negative for Q-PCR, two samples were negative for LAMP but positive for Q-PCR, and four blood samples were negative for LAMP and Q-PCR. LAMP method has an accuracy of 92.50%, with sensitivity and specificity of 94.28% and 80%, respectively. Thus, LAMP diagnostic test has proved reliable, fast, inexpensive and can be useful for detection where the limited resources available.•LAMP method is a potential tool for detection of SARS-CoV-2.•Blood samples are the key determinant for routine diagnostics as well as molecular diagnostics.•LAMP assay is an appropriate diagnostics method which offers greater simplicity, low cost, sensitivity, and specificity than other methods in molecular diagnostics.

International Journal of Productivity and Quality Management ; 37(2):260-283, 2022.
Article in English | Scopus | ID: covidwho-2154332
2nd Asian Conference on Innovation in Technology, ASIANCON 2022 ; 2022.
Article in English | Scopus | ID: covidwho-2136101
Mausam ; 73(4):809-818, 2022.
Article in English | Scopus | ID: covidwho-2081639
Ymer ; 21(2):134-144, 2022.
Article in English | Scopus | ID: covidwho-2057125
Journal of Medical Pharmaceutical and Allied Sciences ; 11(4):5133-5137, 2022.
Article in English | Scopus | ID: covidwho-2030665
2022 International Conference on Sustainable Computing and Data Communication Systems, ICSCDS 2022 ; : 8-14, 2022.
Article in English | Scopus | ID: covidwho-1874306
Journal of Clinical and Diagnostic Research ; 16(4):LC21-LC26, 2022.
Article in English | EMBASE | ID: covidwho-1818675
2021 AICTE Sponsored National Online Conference on Data Science and Intelligent Information Technology ; 2444, 2022.
Article in English | Scopus | ID: covidwho-1795607
J Immunol ; 208(8): 1968-1977, 2022 04 15.
Article in English | MEDLINE | ID: covidwho-1776404


The pandemic of COVID-19 has caused >5 million deaths in the world. One of the leading causes of the severe form of COVID-19 is the production of massive amounts of proinflammatory cytokines. Epigenetic mechanisms, such as histone/DNA methylation, miRNA, and long noncoding RNA, are known to play important roles in the regulation of inflammation. In this study, we investigated if hospitalized COVID-19 patients exhibit alterations in epigenetic pathways in their PBMCs. We also compared gene expression profiles between healthy controls and COVID-19 patients. Despite individual variations, the expressions of many inflammation-related genes, such as arginase 1 and IL-1 receptor 2, were significantly upregulated in COVID-19 patients. We also found the expressions of coagulation-related genes Von Willebrand factor and protein S were altered in COVID-19 patients. The expression patterns of some genes, such as IL-1 receptor 2, correlated with their histone methylation marks. Pathway analysis indicated that most of those dysregulated genes were in the TGF-ß, IL-1b, IL-6, and IL-17 pathways. A targeting pathway revealed that the majority of those altered genes were targets of dexamethasone, which is an approved drug for COVID-19 treatment. We also found that the expression of bone marrow kinase on chromosome X, a member of TEC family kinases, was increased in the PBMCs of COVID-19 patients. Interestingly, some inhibitors of TEC family kinases have been used to treat COVID-19. Overall, this study provides important information toward identifying potential biomarkers and therapeutic targets for COVID-19 disease.

COVID-19 Drug Treatment , COVID-19 , Inflammation , Leukocytes, Mononuclear , COVID-19/genetics , COVID-19/metabolism , DNA Methylation , Epigenesis, Genetic/physiology , Gene Expression , Histones/metabolism , Humans , Inflammation/genetics , Inflammation/metabolism , Leukocytes, Mononuclear/metabolism , Receptors, Interleukin-1/metabolism , Transcriptome