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1.
IEEE Access ; 11:46956-46965, 2023.
Article in English | Scopus | ID: covidwho-20241597

ABSTRACT

Knowledge payment is a new method of electronic learning that has developed in the era of social media. With the impact of the COVID-19 pandemic, the market for knowledge payment is rapidly expanding. Exploring the factors that influence users' sustained willingness is beneficial for better communication between knowledge payment platforms and users, and for achieving a healthier and more sustainable development of the knowledge payment industry. The model of unsustainable usage behavior of knowledge payment users was constructed on the basis of expectation inconsistency theory, price equilibrium theory, and perceived value theory, using the 'cognitive-emotional-behavioral' model framework of cognitive emotion theory. The data were collected from 348 users through a web-based questionnaire and analyzed using structural equation modeling. Findings show that expectation inconsistency, price equilibrium, and quality value, emotional value, and social value have significant effects on discontinuous use intentions. Discontinuous use intentions also significantly affect discontinuous use behavior. © 2013 IEEE.

2.
Academic Journal of Naval Medical University ; 43(11):1268-1273, 2022.
Article in Chinese | EMBASE | ID: covidwho-20241535

ABSTRACT

Objective To investigate the physical and mental condition and medicine consumption of medical staff of shelter hospitals in Shanghai during the fight against the epidemic of coronavirus disease 2019 (COVID-19). Methods A total of 144 frontline medical staff who fighting against the COVID-19 epidemic from a tertiary first-class hospital from Apr. 4 to May 12, 2022 were surveyed by questionnaires online. Their physical condition including body weight change before the medical work and one month later, sleep quality and the medicine consumption during the medical work were collected and analyzed. Results The mean body weight of frontline doctors before the medical work and one month later were (69.80+/-8.35) kg and (68.60+/-7.37) kg, while those of nurses were (55.36+/-8.27) kg and (53.80+/-7.38) kg, both showing a decreasing trend but without significant difference (all P0.05). A total of 63.89% (92/144) frontline medical staff suffered from insomnia, of which 27.08% (39/144) needed drug intervention. The top 5 common diseases among frontline medical staff were sleep disorder (63.89%, 92/144), skin injury (25.69%, 37/144), body pain (23.61%, 34/144), oral ulcer (13.19%, 19/144), and acute upper respiratory tract infection (9.72%, 14/144). A total of 155 medical staff used drugs, and the top 5 common drugs were skin application (16.77%, 26/155), Ganmao'an granule (12.26%, 19/155), zolpidem tartrate tablets (11.61%, 18/155), Kangfuxin liquid (11.61%, 18/155), and celecoxib capsules (8.39%, 13/155). Conclusion Frontline medical staff in shelter hospitals are prone to have body weight change, sleep disorder, skin injury, body pain, oral ulcer, acute upper respiratory tract infection and so on, which suggests that in future similar large-scale medical support, it is necessary to pay sufficient attention to the physical condition of frontline medical staff and provide them sufficient medicine.Copyright © 2022, Second Military Medical University Press. All rights reserved.

3.
Academic Journal of Naval Medical University ; 43(11):1268-1273, 2022.
Article in Chinese | EMBASE | ID: covidwho-2325665

ABSTRACT

Objective To investigate the physical and mental condition and medicine consumption of medical staff of shelter hospitals in Shanghai during the fight against the epidemic of coronavirus disease 2019 (COVID-19). Methods A total of 144 frontline medical staff who fighting against the COVID-19 epidemic from a tertiary first-class hospital from Apr. 4 to May 12, 2022 were surveyed by questionnaires online. Their physical condition including body weight change before the medical work and one month later, sleep quality and the medicine consumption during the medical work were collected and analyzed. Results The mean body weight of frontline doctors before the medical work and one month later were (69.80+/-8.35) kg and (68.60+/-7.37) kg, while those of nurses were (55.36+/-8.27) kg and (53.80+/-7.38) kg, both showing a decreasing trend but without significant difference (all P>0.05). A total of 63.89% (92/144) frontline medical staff suffered from insomnia, of which 27.08% (39/144) needed drug intervention. The top 5 common diseases among frontline medical staff were sleep disorder (63.89%, 92/144), skin injury (25.69%, 37/144), body pain (23.61%, 34/144), oral ulcer (13.19%, 19/144), and acute upper respiratory tract infection (9.72%, 14/144). A total of 155 medical staff used drugs, and the top 5 common drugs were skin application (16.77%, 26/155), Ganmao'an granule (12.26%, 19/155), zolpidem tartrate tablets (11.61%, 18/155), Kangfuxin liquid (11.61%, 18/155), and celecoxib capsules (8.39%, 13/155). Conclusion Frontline medical staff in shelter hospitals are prone to have body weight change, sleep disorder, skin injury, body pain, oral ulcer, acute upper respiratory tract infection and so on, which suggests that in future similar large-scale medical support, it is necessary to pay sufficient attention to the physical condition of frontline medical staff and provide them sufficient medicine.Copyright © 2022, Second Military Medical University Press. All rights reserved.

4.
Medical Journal of Peking Union Medical College Hospital ; 12(1):9-12, 2021.
Article in Chinese | EMBASE | ID: covidwho-2326519

ABSTRACT

Coronavirus disease 2019 (COVID-19), as a public health emergency, is a serious threat to human health. Cancer patients have a high risk of being infected with COVID-19. As one of important means of cancer treatment, radiotherapy has become an important alternative to surgery during the epidemic of COVID-19. The radiotherapy department of Peking Union Medical College Hospital ensured the smooth development of radiotherapy work on the setup of prevention and control systems for COVID-19 by establishing admission strategies for cancer patients, disinfection, isolation, daily management measures, scientific exploration, and clinical practice. In this paper, the associated strategies are summarized and analyzed, which can provide experience and reference for radiotherapy treatment under public health emergencies.Copyright © 2021, Peking Union Medical College Hospital. All rights reserved.

5.
China Biotechnology ; 43(1):71-86, 2023.
Article in Chinese | Scopus | ID: covidwho-2289194

ABSTRACT

Plant bioreactors have been the central part of molecular pharming. Vaccines, antibodies and functional foods produced by plant bioreactors with the benefits of cost-effectiveness, high scalability, rapid production, enabling post-translational modification, and no harmful pathogens contamination are increasingly accepted by the public. In February 2022, Health Canada approved the world's first plant-derived human vaccine Covifenz® for the prevention and treatment of COVID-19, marking the advent of the era of molecular pharming represented by plant bioreactors. This paper elaborates the development history of plant bioreactors, with the main host species representatives of leafy plants and seed plants, the stable and transient expression systems construction for various applications, as well as the enhancement strategies through promoter and codon optimization, "humanization” of glycosylation process, inhibition of gene silencing and protease activity, and also summarize the application of plant-derived protein products, which aim to provide a theoretical and application basis for the development of plant bioreactors. © 2023, China Biotechnology Press. All rights reserved.

6.
2022 IEEE International Conference on Big Data, Big Data 2022 ; : 3063-3070, 2022.
Article in English | Scopus | ID: covidwho-2277243

ABSTRACT

As society grows increasingly more online with each passing year, the problem of cyberbullying becomes more and more prominent, with such incidents having the capacity to negatively impact mental health in a major way, especially among children and teenagers. The proposed approach builds on our previous work that established multi-modal detection of cyberbullying on Twitter, and restructures the multi-modal approach by incorporating social media features such as time-related features and social network information. As a result, the new models reach a classification accuracy between 94.4% and 94.6%, from the previous accuracy of 93%. The proposed new approach affirms the use of context-based data in addition to more directly-related features when analyzing cyberbullying and other interactions with promising improvements. We believe that this work contributes significantly to the study of cyberbullying detection, which is an imminent problem with growing importance in the post-COVID society. © 2022 IEEE.

7.
Kidney International Reports ; 8(3 Supplement):S446-S447, 2023.
Article in English | EMBASE | ID: covidwho-2277235

ABSTRACT

Introduction: The respiratory tract infections (RTIs), including pneumonia, influenza and Coronavirus Disease 2019 (COVID-19), are the leading cause of hospitalization and mortality worldwide, contributing to elevated healthcare and societal costs. There is conflicting evidences about the effects of angiotensin converting enzyme inhibitor (ACEIs) or angiotensin II receptor blockers (ARBs) on the susceptibility of RTIs. Method(s): Systematic review of interventional and observational studies that reported use of ACEI or/and ARB on incidence of pneumonia or influenza or COVID-19. Searching was conducted in the databases of PubMed, Excerpta Medica Database (Embase), Web of Science, Cochrane Central Register of Controlled Trials (CENTRAL), including the Cochrane Library until April 2022, and references of retrieved relevant articles. We assessed the quality of included studies by using Cochrane Collaboration Risk of Bias tool for Randomized Controlled Trials and Newcastle-Ottawa Scale for observational studies. DerSimonian Laird random-effects meta-analysis was conducted to pool effects for the incidence of pneumonia, influenza and COVID-19. Subgroup analyses were carried according to baseline morbidities (hypertension or cardiovascular diseases, cerebrovascular diseases, chronic kidney disease (CKD) and other non-communicable diseases). Pooled estimates of odds ratios (OR) and corresponding 95% confidence intervals (95% CI) were computed, and heterogeneity among studies was assessed using Cochran's Q test and the I2 metrics, with two tailed P values. Result(s): 73 studies met the inclusion criteria, of which 38 studies assessed the odds of pneumonia, 32 studies assessed Covid-19 and 3 studies assessed influenza. The quality of included studies was moderate. Use of ACEIs was associated with a significantly reduced odds of pneumonia (23 studies: OR 0.74, 95% CI 0.64 to 0.85;I2=76.8%), of COVID-19 (24 studies: OR 0.87, 95% CI 0.82 to 0.92;I2=81.9%) and influenza (3 studies: OR 0.75, 95% CI 0.57 to 0.98, I2=97.7%), compared with control treatment. Use of ARBs was also associated with reduced odds of COVID-19 (25 studies: OR 0.90, 95% CI 0.83 to 0.97;I2=91.9%), but not with odds of pneumonia or influenza. These findings remain consistent in the community population, patients with history of cerebrovascular diseases or cardiovascular diseases, but not in those with CKD, diabetes and chronic obstructive pulmonary diseases. Conclusion(s): The current evidence favours a putative protective role of ACEIs, not ARB in odds of pneumonia, COVID-19 and influenza. Patient populations that may benefit most are those within the community, history of cerebrovascular diseases and cardiovascular diseases. No conflict of interestCopyright © 2023

8.
Acta Psychologica Sinica ; 55(2):192-209, 2023.
Article in English | Scopus | ID: covidwho-2246229

ABSTRACT

In our complex social environments, life situations are ever-changing. When dealing with these changes, there is no one-size-fits-all response or regulatory strategy suitable for all situations. Emotion regulation flexibility (ERF)—a framework for understanding individual differences in adaptive responding to ever-changing life contexts—emphasizes that individuals can flexibly deploy and adjust emotion regulation strategies according to specific characteristics of stressful situations in daily life. To achieve regulatory efficacy, it is important that one can utilize a balanced profile of ER strategies and select strategies that fit well with particular stressful situations. Specifically, using multiple ER strategies in daily life, rather than relying on only single-strategies, would indicate higher ERF. Additionally, based on leading models of strategy-situation fit, certain ER strategies are more appropriate for high versus low intensity stressful events. For instance, distraction involves with shielding oneself from negative stimuli and replacing them with irrelevant things, which may have a greater regulatory effect in high-intensity negative situations. Conversely, strategies such as reappraisal, which involves the processing of negative situations through deep cognitive change, may be more effective in lower-intensity negative situations and as a cornerstone of longer-term ER. We used the experience-sampling method (ESM) to quantify individual's ERF;more specifically we assess participants for 1) having more or less balanced ER strategy profiles and 2) showing greater strategy-situation fit, in regard to the use of distraction versus reappraisal in the regulation of high-intensity versus low-intensity negative life events. To test the adaptive value of ERF on negative emotions and mental health, we investigated the influence of ERF on depressive and anxiety symptoms in two samples. We hypothesized that individuals with a more balanced profile of ER strategy use and a great level of strategy-situation fit would have higher levels of mental health, indicated by low levels of anxiety and depressive feelings. In sample 1, two hundred eight college students finished the ESM procedure (2859 beeps). Intensity of negative situations was measured by self-reported negative feelings for the time points where participants reported an adverse event. Simultaneously, we assessed participants' use of two ER strategies (i.e., distraction and reappraisal). Considering the negative impact of COVID-19 on people's daily life, we collected another sample (sample 2, 3462 beeps) with one hundred people who lived in Hubei Province, where Wuhan was in lockdown during the severe phase of COVID-19 (March 7-13, 2020). We measured intensity of negative situations (by averaging individuals' negative feelings), as well as the use of two ER strategies at corresponding time points. After completing the ESM procedure, the participants were asked to fill out a series of emotional health questionnaires, including Beck Depression Inventory-II, Beck Anxiety Inventory and Spielberger State Anxiety Scale. Multilevel models were used to fit the covariation between the use of distraction versus reappraisal ER strategies and the intensity of negative events. Additionally, we used multiple level regression models to test whether high level of strategy-situation fit would result in lower negative feelings. To test whether a single-strategy preference would lead to higher levels of anxiety and depressive feelings compared to a multiple-strategy preference, latent profile analyses (LPA) was used. Results from the LPA indicated that individuals with preferences for rumination and express suppression reported higher levels depression and anxiety than individuals with a multi-strategy preference. In the multilevel models, results of the two independent samples both suggested individuals who were more inclined to use a higher level of distraction in response to high-intensity negative situations (e.g., adverse events or during COVID-19) and use hi her levels of reappraisal during low-intensity situations (i.e., high level of ERF) reported lower levels of anxiety and depressive feelings. On the converse, individuals who tended to use more distraction in low intensity situations and more reappraisal in high intensity situations, (i.e., those showing lower ERF) reported a higher level of negative feelings. Together, our findings revealed a negative relationship between ERF and mental health problems in two samples, suggesting that having balanced ER profiles and flexibly deploying strategies in specific life contexts may have adaptive value in facilitating positive mental health. This work deepens our understanding of the interaction between ER strategies and situational demands, paving the way for future intervention research to help alleviate negative emotions associated with affective disorders or the experience of major traumatic events (such as epidemics, earthquakes, etc.). © The Authors.

9.
Acta Psychologica Sinica ; 55(2):192-209, 2023.
Article in Chinese | Scopus | ID: covidwho-2143833

ABSTRACT

In our complex social environments, life situations are ever-changing. When dealing with these changes, there is no one-size-fits-all response or regulatory strategy suitable for all situations. Emotion regulation flexibility (ERF)—a framework for understanding individual differences in adaptive responding to ever-changing life contexts—emphasizes that individuals can flexibly deploy and adjust emotion regulation strategies according to specific characteristics of stressful situations in daily life. To achieve regulatory efficacy, it is important that one can utilize a balanced profile of ER strategies and select strategies that fit well with particular stressful situations. Specifically, using multiple ER strategies in daily life, rather than relying on only single-strategies, would indicate higher ERF. Additionally, based on leading models of strategy-situation fit, certain ER strategies are more appropriate for high versus low intensity stressful events. For instance, distraction involves with shielding oneself from negative stimuli and replacing them with irrelevant things, which may have a greater regulatory effect in high-intensity negative situations. Conversely, strategies such as reappraisal, which involves the processing of negative situations through deep cognitive change, may be more effective in lower-intensity negative situations and as a cornerstone of longer-term ER. We used the experience-sampling method (ESM) to quantify individual’s ERF;more specifically we assess participants for 1) having more or less balanced ER strategy profiles and 2) showing greater strategy-situation fit, in regard to the use of distraction versus reappraisal in the regulation of high-intensity versus low-intensity negative life events. To test the adaptive value of ERF on negative emotions and mental health, we investigated the influence of ERF on depressive and anxiety symptoms in two samples. We hypothesized that individuals with a more balanced profile of ER strategy use and a great level of strategy-situation fit would have higher levels of mental health, indicated by low levels of anxiety and depressive feelings. In sample 1, two hundred eight college students finished the ESM procedure (2859 beeps). Intensity of negative situations was measured by self-reported negative feelings for the time points where participants reported an adverse event. Simultaneously, we assessed participants’ use of two ER strategies (i.e., distraction and reappraisal). Considering the negative impact of COVID-19 on people’s daily life, we collected another sample (sample 2, 3462 beeps) with one hundred people who lived in Hubei Province, where Wuhan was in lockdown during the severe phase of COVID-19 (March 7-13, 2020). We measured intensity of negative situations (by averaging individuals’ negative feelings), as well as the use of two ER strategies at corresponding time points. After completing the ESM procedure, the participants were asked to fill out a series of emotional health questionnaires, including Beck Depression Inventory-II, Beck Anxiety Inventory and Spielberger State Anxiety Scale. Multilevel models were used to fit the covariation between the use of distraction versus reappraisal ER strategies and the intensity of negative events. Additionally, we used multiple level regression models to test whether high level of strategy-situation fit would result in lower negative feelings. To test whether a single-strategy preference would lead to higher levels of anxiety and depressive feelings compared to a multiple-strategy preference, latent profile analyses (LPA) was used. Results from the LPA indicated that individuals with preferences for rumination and express suppression reported higher levels depression and anxiety than individuals with a multi-strategy preference. In the multilevel models, results of the two independent samples both suggested individuals who were more inclined to use a higher level of distraction in response to high-intensity negative situations (e.g., adverse events or during COVID-19) and use hi her levels of reappraisal during low-intensity situations (i.e., high level of ERF) reported lower levels of anxiety and depressive feelings. On the converse, individuals who tended to use more distraction in low intensity situations and more reappraisal in high intensity situations, (i.e., those showing lower ERF) reported a higher level of negative feelings. Together, our findings revealed a negative relationship between ERF and mental health problems in two samples, suggesting that having balanced ER profiles and flexibly deploying strategies in specific life contexts may have adaptive value in facilitating positive mental health. This work deepens our understanding of the interaction between ER strategies and situational demands, paving the way for future intervention research to help alleviate negative emotions associated with affective disorders or the experience of major traumatic events (such as epidemics, earthquakes, etc.). © The Authors.

10.
Chinese Medicine and Culture ; 4(1):25-30, 2021.
Article in English | Scopus | ID: covidwho-2024703

ABSTRACT

Since the outbreak of coronavirus disease-2019 (COVID-19), traditional Chinese medicine (TCM) has fully displayed its strength and specialty and has played an important role in the prevention and treatment of the pandemic. Faced with the unexpected development of the pandemic, the Chinese government has responded quickly and attached great importance to the effect of TCM. Comprehensive therapy integrating Chinese and Western medicine has achieved remarkable success. To summarize and introduce the prevention and treatment of COVID-19 with TCM, this article covers the perspectives of policy guideline issue, clinical diagnosis participation, and scientific research progress. © 2020 Chinese Medicine and Culture. All rights reserved.

11.
Chinese Journal of Evidence-Based Medicine ; 22(8):932-947, 2022.
Article in Chinese | EMBASE | ID: covidwho-2006473

ABSTRACT

Objective To evaluate the evidence of the experience with medical sewage treatment procedures in medical institutions in China. Methods Databases including CNKI, WanFang Data, PubMed, Web of Science, and EBSCO were electronically searched to collect studies on the medical sewage treatment process, flow, and specifications in medical institutions in China. We used the quality evaluation system to classify and grade the experiences based on the principles and methods of evidence-based science and performed a descriptive analysis. Results After the SARS pandemic in 2003, China systematically established and standardized the technical criteria of medical sewage treatment and discharge. Moreover, a prevention system for the epidemic using medical sewage was constructed, which guaranteed that the quality of medical sewage treatment and discharge would meet the criteria and protect the citizens, and the technical specifications of medical sewage treatment would progress and increase strictly. At present, medical sewage treatment in medical institutions in China was based on mechanical and biological methods, and disinfection was mainly performed using chlorine and its compounds, ozone, and ultraviolet light. Conclusion The COVID-19 pandemic requires a higher quality of medical sewage treatment and discharge criteria for medical institutions in China. To meet these criteria, all medical institutions in China should check, replace, and update their old facilities;strengthen personnel training and effectively ensure the quality of medical sewage treatment.

12.
IEEE Transactions on Affective Computing ; : 1-15, 2022.
Article in English | Scopus | ID: covidwho-1922769

ABSTRACT

The long-lasting global pandemic of Coronavirus disease 2019 (COVID-19) has changed our daily life in many ways and put heavy burden on our mental health. Having a predictive model of negative emotions during COVID-19 is of great importance for identifying potential risky population. To establish a neural predictive model achieving both good interpretability and predictivity, we have utilized a large-scale (n =542) longitudinal dataset, alongside two independent samples for external validation. We built a predictive model based on psychologically meaningful resting state neural activities. The whole-brain resting-state neural activity and social-psychological profile of the subjects were obtained from Sept. to Dec. 2019 (Time 1). Their negative emotions were tracked and re-assessed twice, on Feb 22 (Time 2) and Apr 24 (Time 3), 2020, respectively. We first applied canonical correlation analysis on both the neural profiles and psychological profiles collected on Time 1, this step selects only the psychological meaningful neural patterns for later model construction. We then trained the neural predictive model using those identified features on data obtained on Time 2. It achieved a good prediction performance (r =0.44, p =8.13 ×10-27). The two most important neural predictors are associated with self-control and social interaction. This study established an effective neural prediction model of negative emotions, achieving good interpretability and predictivity. It will be useful for identifying potential risky population of emotional disorders related to COVID-19. IEEE

13.
Food Science and Technology ; 42:8, 2022.
Article in English | Web of Science | ID: covidwho-1917069

ABSTRACT

Alcoholic beverages play an important role in social gatherings and the consumption of alcohol drinks keep increasing worldwide in recent years, especially during the COVID-19 pandemic months. The authentication of alcoholic beverage is usually evaluated by trained panels or chromatography analysis. Over the last few decades, intelligent sensory technology (IST) that imitate the human sensory organs have been developed for quality control and authentication of alcoholic beverages. The artificial sensing system consist of arrays of sensors with cross-sensitivity and various pattern recognition methods, which can be used to discriminate or classify the samples based on the detection requirements. Application of IST on wine authenticity have been extensively studied, however, application of IST in authentication of other alcoholic beverages lacks of systemic study. This paper firstly describes the basic mechanism of current IST instruments and then summarizes the applications of IST in alcoholic beverages authenticity assessments, including discrimination of varietal and geographical origins, detection of frauds and adulterations, discrimination of years of aging, distinction of brands and types, aroma analysis, detection of spoilage and off-flavors, and monitoring of the production process. The potential applications and future development of IST in the brewing industry are also discussed.

14.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 34(2): 149-157, 2022 Apr 07.
Article in Chinese | MEDLINE | ID: covidwho-1893448

ABSTRACT

OBJECTIVE: To examine the effects of Toxoplasma gondii infection on the proportion, quantity, differentiation and function of mouse and human uterine natural killer cells (uNK cells), so as to explore the role of uNK cells in abortion of early pregnancy caused by T. gondii infection. METHODS: Pregnant mice were injected intraperitoneally with T. gondii tachyzoites on day 6.5 of pregnancy, and the abortion mouse model caused by T. gondii infections was constructed. Mouse uterine lymphocytes were isolated on day 9.5 of pregnancy. Human uterine lymphocytes were isolated from fresh human decidual specimens after abortion in normal early pregnancy and co-cultured with tachyzoites of the T. gondii RH strain for 48 h at T. gondii/uterine lymphocytes ratios of 0.5:1, 1:1 and 2:1. The phenotypes of mouse uNK cells (CD122, NK1.1, DX5) and human uNK cells (CD3, CD56, CD11b, CD27) and the expression of intracellular cytokines interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were detected by flow cytometry. Mouse and human uNK cells were sorted by magnetic beads, and the cytotoxicity of uNK cells was tested using the lactate dehydrogenase (LDH) release assay at effector/target cell ratios of 1:1, 5:1, 10:1 and 20:1 with mouse or human uNK cells as effector cells and mouse YAC-1 cells or human K562 cells as target cells. RESULTS: On day 9.5 of pregnancy, the mouse abortion rate was significantly higher in the infected group than that in the control group (83.02% vs. 3.51%; χ2 = 71.359, P < 0.001). Significantly lower absolute number of uNK cells [(4 547 ± 1 610) cells/mouse vs. (8 978 ± 3 339) cells/mouse; U = 2.000, P < 0.05], lower NK1.1 expression on uNK cell surface [(74.53 ± 8.37)% vs. (93.00 ± 1.11)%; U = 0.000, P < 0.05], higher proportion of NK1.1-DX5-cells [(20.10 ± 8.03)% vs. (5.04 ± 0.68)%; U = 0.000, P < 0.05], lower proportion of NK1.1+ DX5+ cells [(21.70 ± 12.48)% vs. (45.75 ± 2.26)%; U = 0.000, P < 0.05] and higher IFN-γ expression [(16.74 ± 1.36)% vs. (8.13 ± 1.90)%; U = 0.000, P < 0.05] were detected in the infected group than in the control group, while no significant difference was seen in TNF-α expression between the two groups [(67.98 ± 9.20)% vs. (52.93 ± 10.42)%; U = 2.000, P > 0.05]. The mouse uNK cells showed a strong cytotoxicity in the infected group, and the cytotoxicity gradually increased with the effector/target cell ratio. The cytotoxicity of uNK cells against YAC-1 cells was 2.30%, 4.32%, 8.12% and 12.65% in the infected group and 1.21%, 1.63%, 2.51% and 3.22% in the control group at effector/target cell ratios of 1:1, 5:1, 10:1 and 20:1, respectively. Following co-culture of human uterine lymphocytes and tachyzoites of the T. gondii RH strain for 48 h, the proportion [TOX 2:1 group vs. control group: (6.61 ± 1.75)% vs. (17.48 ± 4.81)%; F = 7.307, P < 0.01], and absolute number of human uNK cells in uterine lymphocytes of human uNK cells in uterine lymphocytes [TOX 2:1 group vs. control group: (12 104 ± 5 726) cells/well vs. (65 285 ± 21 810) cells/well; H = 11.540, P < 0.01] were significantly lower in the infected group than in the control group. A lower proportion of CD56brightCD16- NK cells [TOX 2:1 group vs. control group: (25.25 ± 5.90)% vs. (36.03 ± 4.51)%; F = 3.213, P > 0.05] and higher proportion of CD56dimCD16+ NK cells [TOX 2:1 group vs. control group: (11.15 ± 2.15)% vs. (7.09 ± 2.24)%; F = 2.992, P > 0.05] were detected in uNK cells in the infected group than in the control group, and the ratio of CD56brightCD16- cells/CD56dimCD16+ cells was significantly lower in the infected group than in the control group [TOX2:1 group vs. control group: (2.37 ± 0.92) vs. (5.58 ± 2.39); H = 8.228, P < 0.05]. In addition, the proportion of CD11b+CD27- cells in human uNK cells was significantly higher in the infected group than in the control group [TOX 2:1 group vs. control group: (30.28 ± 6.91)% vs. (17.48 ± 4.67)%; H = 6.556, P < 0.05], while no significant differences were found between the two groups in terms of IFN-γ [TOX 2:1 group vs. control group: (14.13 ± 1.28)% vs. (15.19 ± 1.64)%; F = 1.639, P > 0.05] or TNF-α expression [TOX 2:1 group vs. control group: (54.76 ± 10.02)% vs. (50.33 ± 3.67)%; F = 0.415, P > 0.05]. Human uNK cells presented a strong cytotoxicity in the infected group, and the cytotoxicity gradually increased with the effector/target cell ratio. The cytotoxicity of human uNK cells against K562 cells was 11.90%, 28.11%, 49.91% and 73.35% in the infected group and 12.21%, 21.63%, 33.51% and 48.22% in the control group at effector/target cell ratios of 1:1, 5:1, 10:1 and 20:1, respectively. CONCLUSIONS: T. gondii infection presents diverse effects on the differentiation and secretion ability of mouse and human uNK cells. However, T. gondii infection causes a reduction in the absolute number and enhances the cytotoxicity of both mouse and human uNK cells.


Subject(s)
Abortion, Spontaneous , Toxoplasma , Toxoplasmosis , Female , Humans , Interferon-gamma/genetics , Killer Cells, Natural/pathology , Pregnancy , Tumor Necrosis Factor-alpha/genetics
15.
New England Journal of Medicine ; 386(4):402-403, 2022.
Article in English | Web of Science | ID: covidwho-1710916
16.
2021 International Conference on Artificial Intelligence and Big Data Applications, ICAIBD 2021 ; 2138, 2021.
Article in English | Scopus | ID: covidwho-1672071

ABSTRACT

The sudden outbreak of COVID-19 has caused great losses to the economy and the life of the masses. Long short-term memory (LSTM) network is a time recursive neural network, which is suitable for processing and predicting important events with relatively long interval and delay in time series. Using LSTM network to predict and analyze the development trend of epidemic situation, it is imperative to prevent epidemic situation from causing secondary harm to China's development. In this paper, we first obtained the COVID-19 data published by China Health Net using crawler technology, which is the accurate value of infection trend after the outbreak of COVID-19 in China. Then, based on these data, the LSTM model is used to predict the development trend of the epidemic in one year, and the mean square error is used to calculate the error between the prediction and the real data. The experimental model is used to predict and analyze the development trend of COVID-19. The results show that the error between predicted data and real data is small and the effect is very good, which provides a reasonable basis and forecast for scientific prevention and control of epidemic situation. © Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Published under licence by IOP Publishing Ltd

17.
Zhonghua Yu Fang Yi Xue Za Zhi ; 55(12): 1377-1382, 2021 Dec 06.
Article in Chinese | MEDLINE | ID: covidwho-1600026

ABSTRACT

Since the Global Polio Eradication Initiative was launched by the World Health Assembly in 1988, significant progress has been made in global polio prevention and control. But the occurrence of vaccine-associated paralytic poliomyelitis cases and vaccine-derived poliovirus related cases have become a major challenge during the post-polio era. While coronavirus disease 2019(COVID-19) has brought serious disease burden and economic burden to all countries in the world, prevention and control of vaccine-preventable infectious diseases such as polio should not be neglected under the background of the global common fight against COVID-19. Taking the type Ⅲ VDPV cycle event in Shanghai as an example, the paper discussed how to do a good job of routine inoculation under the prevention and control of COVID-19 to strictly prevent the outbreak of vaccine-preventable infectious diseases.


Subject(s)
COVID-19 , Poliovirus , China , Humans , Poliovirus Vaccine, Oral , SARS-CoV-2 , Vaccination
18.
Medical Journal of Peking Union Medical College Hospital ; 12(1):9-12, 2021.
Article in Chinese | Scopus | ID: covidwho-1513194

ABSTRACT

Coronavirus disease 2019 (COVID-19), as a public health emergency, is a serious threat to human health. Cancer patients have a high risk of being infected with COVID-19. As one of important means of cancer treatment, radiotherapy has become an important alternative to surgery during the epidemic of COVID-19. The radiotherapy department of Peking Union Medical College Hospital ensured the smooth development of radiotherapy work on the setup of prevention and control systems for COVID-19 by establishing admission strategies for cancer patients, disinfection, isolation, daily management measures, scientific exploration, and clinical practice. In this paper, the associated strategies are summarized and analyzed, which can provide experience and reference for radiotherapy treatment under public health emergencies. © 2021, Peking Union Medical College Hospital. All rights reserved.

19.
Journal of Shanghai Jiaotong University (Medical Science) ; 41(10):1359-1365, 2021.
Article in Chinese | EMBASE | ID: covidwho-1497436

ABSTRACT

Objective: ·To explore the reliability and validity of Corona Virus Disease 2019 (COVID-19) Peritraumatic Distress Index (CPDI) in evaluating pandemic-related acute stress reaction. Methods: ·From 31 January 2020 to 29 February 2020, CPDI, Patient Health Questionnaire-9 (PHQ-9), General Anxiety Disorder-7 (GAD-7) and Self-reporting Questionnaire-20 (SRQ-20) were measured in 253 outpatients, their family members and medical staff of our hospital. At the same time, leveraging the Siuvo Intelligent Psychological Assessment Platform, we presented QR codes of the CPDI questionnaire online openly, accessible to the participants nationwide, and received a total of 75 867 valid responses. Cronbach's α coefficient was used to evaluate the internal consistency. The correlation between CPDI and PHQ-9, GAD-7 and SRQ-20 was used to test the criterion validity. Exploratory factor analysis was used to detect the structural validity and the cut-off score of the questionnaire to screen the psychological symptoms associated with COVID-19 was also detected. Results: ·The Cronbach's α of the 15-item questionnaire was 0.932. The correlation coefficients between the 15-item CPDI and PHQ-9, GAD-7 and SRQ-20 were 0. 670, 0.699 and 0.711 respectively (all P=0.000). The exploratory factor analysis results contained 15 items and 4 factors, the factor load was 0.491-0.681, and the cumulative variance interpretation rate was 72.006%. In terms of cut-off score, it was identified as suspicious symptom by cut-off score of 10/11 points. The sensitivity and specificity were 0.822 and 0.811, respectively. The cut-off score to confirm the presence of symptoms was 14/15 points. The sensitivity and specificity were 0.815 and 0.824, respectively. Conclusion: ·The study indicates that CPDI is with good internal consistency reliability, criterion validity and structural validity.

20.
Medicine and Science in Sports and Exercise ; 53(8):187-187, 2021.
Article in English | Web of Science | ID: covidwho-1436868
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