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J Virol Methods ; 293: 114131, 2021 Mar 30.
Article in English | MEDLINE | ID: covidwho-1157565


The World Health Organization (WHO) has declared a pandemic of COVID-19, the disease caused by the recently described SARS-CoV-2. The relevance and importance of mass diagnosis in order to find the asymptomatic individuals is widely recognized as a mandatory tool to reinforce the control measures for monitoring virus circulation and reduce the spreading of SARS-CoV-2. Here, we described quickness and cheaper strategies of direct RT-qPCR (in the absence of RNA isolation) and compared the results to those obtained using standard RNA isolation procedure. The tests varied using pure, diluted samples, combined with Proteinase K (PK) or Lysis Buffer. Our findings showed consistently that PK pre-treated samples in the absence of RNA extraction procedures presents similar results to those obtained by standard RNA isolation procedures. On average, 16 samples extracted with the MagMAX™ CORE Kit, take around 2 h, costing an average of USD 5, the pre-treatment of samples using PK, on the other hand, would cut the value to less than USD 0.30 and reduce the time of procedure in more than 1 ½ hours. The present study suggests the use of PK treatment instead of RNA isolation in order to reduce costs and time in processing samples for molecular diagnosis of SARS-CoV-2.

Genetics & Molecular Biology ; 44(1 Suppl 1):e20200228, 2021.
Article in English | WHO COVID | ID: covidwho-1133734


December 2019 marked the beginning of the current Coronavirus disease pandemic (COVID-19) Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) was identified as the causative agent of a viral pneumonia outbreak in Wuhan, Hubei Province, China The alarming spread levels and clinical severity elevated the status of COVID-19 to the global pandemic by the World Health Organization In 6 months, more than 25 million cases of infected people and more than 890,000 deaths by COVID-19 had been reported worldwide The main goal of this review is to shed light upon the current COVID-19 epidemic situation in Brazil with a health approach highlighting some unique environmental, animal and epidemiological aspects

Bone Marrow Transplant ; : 1-9, 2020.
Article | WHO COVID | ID: covidwho-939434


The COVID-19 pandemic has serious implications also for patients with other diseases Here, we describe the effects of the pandemic on unrelated hematopoietic stem cell donation and transplantation from the perspective of DKMS, a large international donor registry Especially, we cover the development of PBSC and bone marrow collection figures, donor management including Health and Availability Check (HAC), transport and cryopreservation of stem cell products, donor recruitment and business continuity measures The total number of stem cell products provided declined by around 15% during the crisis with a particularly strong decrease in bone marrow products We modified donor management processes to ensure donor and product safety HAC instead of confirmatory typing was helpful especially in countries with strict lockdowns New transport modes were developed so that stem cell products could be safely delivered despite COVID-19-related travel restrictions Cryopreservation of stem cell products became the new temporary standard during the pandemic to minimize risks related to transport logistics and donor availability However, many products from unrelated donors will never be transfused DKMS discontinued public offline donor recruitment, leading to a 40% decline in new donors during the crisis Most DKMS employees worked from home to ensure business continuity during the crisis

Radiologe ; 60(10):891-892, 2020.
Article | WHO COVID | ID: covidwho-807578