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1.
Viruses ; 14(12):2738, 2022.
Article in English | MDPI | ID: covidwho-2155311

ABSTRACT

Influenza virus infections and the continuing spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are global public health concerns. As there are limited therapeutic options available in clinical practice, the rapid development of safe, effective and globally available antiviral drugs is crucial. Drug repurposing is a therapeutic strategy used in treatments for newly emerging and re-emerging infectious diseases. It has recently been shown that the voltage-dependent Ca2+ channel Cav1.2 is critical for influenza A virus entry, providing a potential target for antiviral strategies. Nisoldipine, a selective Ca2+ channel inhibitor, is commonly used in the treatment of hypertension. Here, we assessed the antiviral potential of nisoldipine against the influenza A virus and explored the mechanism of action of this compound. We found that nisoldipine treatment could potently inhibit infection with multiple influenza A virus strains. Mechanistic studies further revealed that nisoldipine impaired the internalization of the influenza virus into host cells. Overall, our findings demonstrate that nisoldipine exerts antiviral effects against influenza A virus infection and could serve as a lead compound in the design and development of new antivirals.

2.
International Journal of Environmental Research and Public Health ; 19(24):16433, 2022.
Article in English | MDPI | ID: covidwho-2155078

ABSTRACT

Urban rail transit (URT) is a key mode of public transport, which serves for greatest user demand. Short-term passenger flow prediction aims to improve management validity and avoid extravagance of public transport resources. In order to anticipate passenger flow for URT, managing nonlinearity, correlation, and periodicity of data series in a single model is difficult. This paper offers a short-term passenger flow prediction combination model based on complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN) and long-short term memory neural network (LSTM) in order to more accurately anticipate the short-period passenger flow of URT. In the meantime, the hyperparameters of LSTM were calculated using the improved particle swarm optimization (IPSO). First, CEEMDAN-IPSO-LSTM model performed the CEEMDAN decomposition of passenger flow data and obtained uncoupled intrinsic mode functions and a residual sequence after removing noisy data. Second, we built a CEEMDAN-IPSO-LSTM passenger flow prediction model for each decomposed component and extracted prediction values. Third, the experimental results showed that compared with the single LSTM model, CEEMDAN-IPSO-LSTM model reduced by 40 persons/35 persons, 44 persons/35 persons, 37 persons/31 persons, and 46.89%/35.1% in SD, RMSE, MAE, and MAPE, and increase by 2.32%/3.63% and 2.19%/1.67% in R and R2, respectively. This model can reduce the risks of public health security due to excessive crowding of passengers (especially in the period of COVID-19), as well as reduce the negative impact on the environment through the optimization of traffic flows, and develop low-carbon transportation.

3.
Viruses ; 14(10)2022 10 09.
Article in English | MEDLINE | ID: covidwho-2143671

ABSTRACT

For industrial vaccine production, overwhelming the existing antiviral innate immune response dominated by type I interferons (IFN-I) in cells would be a key factor improving the effectiveness and production cost of vaccines. In this study, we report the construction of an IFN-I receptor 1 (IFNAR1)-knockout DF-1 cell line (KO-IFNAR1), which supports much more efficient replication of the duck Tembusu virus (DTMUV), Newcastle disease virus (NDV) and gammacoronavirus infectious bronchitis virus (IBV). Transcriptomic analysis of DTMUV-infected KO-IFNAR1 cells demonstrated that DTMUV mainly activated genes and signaling pathways related to cell growth and apoptosis. Among them, JUN, MYC and NFKBIA were significantly up-regulated. Furthermore, knockdown of zinc-fingered helicase 2 (HELZ2) and interferon-α-inducible protein 6 (IFI6), the two genes up-regulated in both wild type and KO-IFNAR1 cells, significantly increased the replication of DTMUV RNA. This study paves the way for further studying the mechanism underlying the DTMUV-mediated IFN-I-independent regulation of virus replication, and meanwhile provides a potential cell resource for efficient production of cell-based avian virus vaccines.


Subject(s)
Flavivirus Infections , Flavivirus , Interferon Type I , Poultry Diseases , Animals , Ducks , Chickens/genetics , Transcriptome , Flavivirus/genetics , Cell Line , Interferon Type I/genetics , Antiviral Agents , Apoptosis , RNA , Interferon-alpha/genetics , Zinc
4.
Genes & Diseases ; 2022.
Article in English | ScienceDirect | ID: covidwho-2130871

ABSTRACT

Inactivated COVID-19 vaccines have been widely used to vaccinate the Chinese population. However, limited literature exists to explore the effect of obesity on the humoral and cellular immune response to these vaccines. In this study, 132 high BMI (Body mass index) (obesity and overweight, BMI ≥ 24 kg/m2) and 82 normal BMI (BMI < 24 kg/m2) participants were enrolled. Adverse events (AEs), Spike receptor-binding domain IgG antibody (anti-RBD-IgG), neutralizing antibodies (NAbs), and specific B-cell and T-cell responses were evaluated 21-105 days after full-course inactivated COVID-19 vaccination. The overall incidence of adverse events (AEs) was similar in individuals with and without obesity/overweight. No serious vaccine-related AEs occurred. Individuals with obesity/overweight had a reduced seropositivity rate of NAbs compared to those with normal BMI. Anti-RBD-IgG and NAbs titers in the high BMI group were significantly lower than those in the normal BMI group. The frequencies of RBD-specific memory B cells (MBCs) and the numbers of spike-specific TNF-α+ spot-forming cells (SFCs) in individuals with obesity/overweight were reduced compared with those noted in individuals without obesity/overweight. A similar trend of weakened humoral responses was also observed in individuals with central obesity. Our study results suggested that inactivated COVID-19 vaccines were safe and well tolerated but induced poor humoral and cellular immune responses in Chinese individuals with obesity/overweight.

5.
Medicine (Baltimore) ; 101(45): e31740, 2022 Nov 11.
Article in English | MEDLINE | ID: covidwho-2115861

ABSTRACT

This paper mainly discusses how to do a good job of daily biosafety protection measures in clinical microbiology laboratories during the epidemic of COVID-19, so as to ensure the safe development of routine clinical microbiology testing items. According to the microbiological and epidemiological characteristics of the novel coronavirus, this paper analyzed the potential risks of the laboratory from the perspective of personal protection before, during, and after testing. Combined with the actual work situation, the improved biosafety protection measures and optimized work flow are introduced to ensure the safety of medical staff and the smooth development of daily work. Danyang People's Hospital of Jiangsu Province, clinical microbiology laboratory of clinical laboratory in strict accordance with the relevant laws and regulations, technical specifications and the expert consensus, combined with their own conditions, the biosafety measures to perfect the working process was optimized, effectively prevent the laboratory exposure, and maintain strict working condition for a long time, continue to improve. We found that the biosafety protection measures of clinical microbiology laboratory have good prevention and control effect on preventing infection of medical staff, which will greatly reduce the risk of infection of medical staff, form good working habits, and provide reference for biosafety protection of microbiology laboratory during the epidemic of COVID-19.


Subject(s)
COVID-19 , Clinical Laboratory Services , Humans , Containment of Biohazards , Laboratories , Workflow
6.
researchsquare; 2022.
Preprint in English | PREPRINT-RESEARCHSQUARE | ID: ppzbmed-10.21203.rs.3.rs-2369060.v1

ABSTRACT

A series of 4-methyl-5-(3-phenylacryloyl)thiazoles based on chalcones were designed, synthesized and evaluated for their influenza neuraminidase (NA) inhibitory activity in vitro.  A preliminary structure–activity relationship (SAR) analysis showed that thiazoles bearing amide had greater potency. It also showed that mono-hydroxyl group at 4-position on phenyl ring was more effective than other electron-releasing groups or electron-withdraw groups. Compounds A2 and A26 were more potent against NA with IC50 values of 8.2±0.5 μg/mL and 6.2±1.4 μg/mL, respectively. Molecular docking study demonstrated that thiazoles skeleton was benefit for the NA inhibitory activity.

7.
J Med Internet Res ; 24(10): e39676, 2022 10 13.
Article in English | MEDLINE | ID: covidwho-2109563

ABSTRACT

BACKGROUND: The COVID-19 pandemic and its corresponding preventive and control measures have increased the mental burden on the public. Understanding and tracking changes in public mental status can facilitate optimizing public mental health intervention and control strategies. OBJECTIVE: This study aimed to build a social media-based pipeline that tracks public mental changes and use it to understand public mental health status regarding the pandemic. METHODS: This study used COVID-19-related tweets posted from February 2020 to April 2022. The tweets were downloaded using unique identifiers through the Twitter application programming interface. We created a lexicon of 4 mental health problems (depression, anxiety, insomnia, and addiction) to identify mental health-related tweets and developed a dictionary for identifying health care workers. We analyzed temporal and geographic distributions of public mental health status during the pandemic and further compared distributions among health care workers versus the general public, supplemented by topic modeling on their underlying foci. Finally, we used interrupted time series analysis to examine the statewide impact of a lockdown policy on public mental health in 12 states. RESULTS: We extracted 4,213,005 tweets related to mental health and COVID-19 from 2,316,817 users. Of these tweets, 2,161,357 (51.3%) were related to "depression," whereas 1,923,635 (45.66%), 225,205 (5.35%), and 150,006 (3.56%) were related to "anxiety," "insomnia," and "addiction," respectively. Compared to the general public, health care workers had higher risks of all 4 types of problems (all P<.001), and they were more concerned about clinical topics than everyday issues (eg, "students' pressure," "panic buying," and "fuel problems") than the general public. Finally, the lockdown policy had significant associations with public mental health in 4 out of the 12 states we studied, among which Pennsylvania showed a positive association, whereas Michigan, North Carolina, and Ohio showed the opposite (all P<.05). CONCLUSIONS: The impact of COVID-19 and the corresponding control measures on the public's mental status is dynamic and shows variability among different cohorts regarding disease types, occupations, and regional groups. Health agencies and policy makers should primarily focus on depression (reported by 51.3% of the tweets) and insomnia (which has had an ever-increasing trend since the beginning of the pandemic), especially among health care workers. Our pipeline timely tracks and analyzes public mental health changes, especially when primary studies and large-scale surveys are difficult to conduct.


Subject(s)
COVID-19 , Sleep Initiation and Maintenance Disorders , Social Media , COVID-19/epidemiology , COVID-19/prevention & control , Communicable Disease Control , Humans , Infodemiology , Mental Health , Pandemics/prevention & control , Policy
8.
EBioMedicine ; 86: 104341, 2022 Nov 11.
Article in English | MEDLINE | ID: covidwho-2104818

ABSTRACT

BACKGROUND: The COVID-19 pandemic continues to cause morbidity and mortality worldwide. Most approved COVID-19 vaccines generate a neutralizing antibody response that primarily targets the highly variable receptor-binding domain (RBD) of the SARS-CoV-2 spike (S) protein. SARS-CoV-2 "variants of concern" have acquired mutations in this domain allowing them to evade vaccine-induced humoral immunity. Recent approaches to improve the breadth of protection beyond SARS-CoV-2 have required the use of mixtures of RBD antigens from different sarbecoviruses. It may therefore be beneficial to develop a vaccine in which the protective immune response targets a more conserved region of the S protein. METHODS: Here we have developed a vaccine based on the conserved S2 subunit of the S protein and optimized the adjuvant and immunization regimen in Syrian hamsters and BALB/c mice. We have characterized the efficacy of the vaccine against SARS-CoV-2 variants and other coronaviruses. FINDINGS: Immunization with S2-based constructs elicited a broadly cross-reactive IgG antibody response that recognized the spike proteins of not only SARS-CoV-2 variants, but also SARS-CoV-1, and the four endemic human coronaviruses. Importantly, immunization reduced virus titers in respiratory tissues in vaccinated animals challenged with SARS-CoV-2 variants B.1.351 (beta), B.1.617.2 (delta), and BA.1 (omicron) as well as a pangolin coronavirus. INTERPRETATION: These results suggest that S2-based constructs can elicit a broadly cross-reactive antibody response resulting in limited virus replication, thus providing a framework for designing vaccines that elicit broad protection against coronaviruses. FUNDING: NIH, Japan Agency for Medical Research and Development, Garry Betty/ V Foundation Chair Fund, and NSF.

9.
Journal of Tropical Medicine ; 21(3):320-323, 2021.
Article in Chinese | GIM | ID: covidwho-2073985

ABSTRACT

Objective: To analyze the change characteristics of peripheral blood leukocyte classification and T lymphocyte subsets in patients. with corona virus disease 2019(COVID-19).

10.
Nat Chem Biol ; 2022 Sep 22.
Article in English | MEDLINE | ID: covidwho-2036839

ABSTRACT

Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 13 (Cas13) has been rapidly developed for nucleic-acid-based diagnostics by using its characteristic collateral activity. Despite the recent progress in optimizing the Cas13 system for the detection of nucleic acids, engineering Cas13 protein with enhanced collateral activity has been challenging, mostly because of its complex structural dynamics. Here we successfully employed a novel strategy to engineer the Leptotrichia wadei (Lwa)Cas13a by inserting different RNA-binding domains into a unique active-site-proximal loop within its higher eukaryotes and prokaryotes nucleotide-binding domain. Two LwaCas13a variants showed enhanced collateral activity and improved sensitivity over the wild type in various buffer conditions. By combining with an electrochemical method, our variants detected the SARS-CoV-2 genome at attomolar concentrations from both inactive viral and unextracted clinical samples, without target preamplification. Our engineered LwaCas13a enzymes with enhanced collateral activity are ready to be integrated into other Cas13a-based platforms for ultrasensitive detection of nucleic acids.

11.
Int J Environ Res Public Health ; 19(17)2022 Sep 02.
Article in English | MEDLINE | ID: covidwho-2010032

ABSTRACT

The conversion rate between asymptomatic infections and reported/unreported symptomatic infections is a very sensitive parameter for model variables that spread COVID-19. This is important information for follow-up use in screening, prediction, prognostics, contact tracing, and drug development for the COVID-19 pandemic. The model described here suggests that there may not be enough researchers to solve all of these problems thoroughly and effectively, and it requires careful selection of what we are doing and rapid sharing of results and models and optimizing modeling simulations with value to reduce the impact of COVID-19. Exploring simulation modeling will help decision makers make the most informed decisions. In order to fight against the "Delta" virus, the establishment of a line of defense through all-people testing (APT) is not only an effective method summarized from past experience but also one of the best means to effectively cut the chain of epidemic transmission. The effect of large-scale testing has been fully verified in the international community. We developed a practical dynamic infectious disease model-SETPG (A + I) RD + APT by considering the effects of the all-people test (APT). The model is useful for studying effects of screening measures and providing a more realistic modelling with all-people-test strategies, which require everybody in a population to be tested for infection. In prior work, a total of 370 epidemic cases were collected. We collected three kinds of known cases: the cumulative number of daily incidences, daily cumulative recovery, and daily cumulative deaths in Hong Kong and the United States between 22 January 2020 and 13 November 2020 were simulated. In two essential strategies of the integrated SETPG (A + I) RD + APT model, comparing the cumulative number of screenings in derivative experiments based on daily detection capability and tracking system application rate, we evaluated the performance of the timespan required for the basic regeneration number (R0) and real-time regeneration number (R0t) to reach 1; the optimal policy of each experiment is available, and the screening effect is evaluated by screening performance indicators. with the binary encoding screening method, the number of screenings for the target population is 8667 in HK and 1,803,400 in the U.S., including 6067 asymptomatic cases in HK and 1,262,380 in the U.S. as well as 2599 cases of mild symptoms in HK and 541,020 in the U.S.; there were also 8.25 days of screening timespan in HK and 9.25 days of screening timespan required in the U.S. and a daily detectability of 625,000 cases in HK and 6,050,000 cases in the U.S. Using precise tracking technology, number of screenings for the target population is 6060 cases in HK and 1,766,420 cases in the U.S., including 4242 asymptomatic cases in HK and 1,236,494 cases in the U.S. as well as 1818 cases of mild symptoms in HK and 529,926 cases in the U.S. Total screening timespan (TS) is 8.25~9.25 days. According to the proposed infectious dynamics model that adapts to the all-people test, all of the epidemic cases were reported for fitting, and the result seemed more reasonable, and epidemic prediction became more accurate. It adapted to densely populated metropolises for APT on prevention.


Subject(s)
COVID-19 , Communicable Diseases , COVID-19/diagnosis , COVID-19/epidemiology , COVID-19 Testing , Communicable Diseases/epidemiology , Humans , Pandemics/prevention & control , SARS-CoV-2 , United States
12.
Cell Discov ; 8(1): 86, 2022 Sep 06.
Article in English | MEDLINE | ID: covidwho-2008267

ABSTRACT

The ongoing COVID-19 pandemic has continued to affect millions of lives worldwide, leading to the urgent need for novel therapeutic strategies. G-quadruplexes (G4s) have been demonstrated to regulate life cycle of multiple viruses. Here, we identify several highly conservative and stable G4s in SARS-CoV-2 and clarify their dual-function of inhibition of the viral replication and translation processes. Furthermore, the cationic porphyrin compound 5,10,15,20-tetrakis-(N-methyl-4-pyridyl)porphine (TMPyP4) targeting SARS-CoV-2 G4s shows excellent antiviral activity, while its N-methyl-2-pyridyl positional isomer TMPyP2 with low affinity for G4 has no effects on SARS-CoV-2 infection, suggesting that the antiviral activity of TMPyP4 attributes to targeting SARS-CoV-2 G4s. In the Syrian hamster and transgenic mouse models of SARS-CoV-2 infection, administration of TMPyP4 at nontoxic doses significantly suppresses SARS-CoV-2 infection, resulting in reduced viral loads and lung lesions. Worth to note, the anti-COVID-19 activity of TMPyP4 is more potent than remdesivir evidenced by both in vitro and in vivo studies. Our findings highlight SARS-CoV-2 G4s as a novel druggable target and the compelling potential of TMPyP4 for COVID-19 therapy. Different from the existing anti-SARS-CoV-2 therapeutic strategies, our work provides another alternative therapeutic tactic for SARS-CoV-2 infection focusing on targeting the secondary structures within SARS-CoV-2 genome, and would open a new avenue for design and synthesis of drug candidates with high selectivity toward the new targets.

14.
Angewandte Chemie ; 134(32), 2022.
Article in English | ProQuest Central | ID: covidwho-1981566

ABSTRACT

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas) systems have recently received notable attention for their applications in nucleic acid detection. Despite many attempts, the majority of current CRISPR‐based biosensors in infectious respiratory disease diagnostic applications still require target preamplifications. This study reports a new biosensor for amplification‐free nucleic acid detection via harnessing the trans‐cleavage mechanism of Cas13a and ultrasensitive graphene field‐effect transistors (gFETs). CRISPR Cas13a‐gFET achieves the detection of SARS‐CoV‐2 and respiratory syncytial virus (RSV) genome down to 1 attomolar without target preamplifications. Additionally, we validate the detection performance using clinical SARS‐CoV‐2 samples, including those with low viral loads (Ct value >30). Overall, these findings establish our CRISPR Cas13a‐gFET among the most sensitive amplification‐free nucleic acid diagnostic platforms to date.

15.
Angewandte Chemie (International ed. in English) ; 61(32), 2022.
Article in English | EuropePMC | ID: covidwho-1980079

ABSTRACT

Nucleic acid detection plays a critical role in medical diagnostics, environmental monitoring, and food safety. In their Research Article (e202203826), Xue Gao, Yi Zhang and co‐workers developed a new biosensor for amplification‐free nucleic acid detection via harnessing the trans‐cleavage mechanism of Cas13a and ultrasensitive graphene field‐effect transistors (gFETs). The illustration shows the Cas13a‐mediated RNA trans‐cleavage on a gFET surface for sensor signal transduction.

16.
Int J Mol Sci ; 23(16)2022 Aug 14.
Article in English | MEDLINE | ID: covidwho-1981428

ABSTRACT

The emergence of phytopathogenic bacteria resistant to antibacterial agents has rendered previously manageable plant diseases intractable, highlighting the need for safe and environmentally responsible agrochemicals. Inhibition of bacterial cell division by targeting bacterial cell division protein FtsZ has been proposed as a promising strategy for developing novel antibacterial agents. We previously identified 4'-demethylepipodophyllotoxin (DMEP), a naturally occurring substance isolated from the barberry species Dysosma versipellis, as a novel chemical scaffold for the development of inhibitors of FtsZ from the rice blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). Therefore, constructing structure-activity relationship (SAR) studies of DMEP is indispensable for new agrochemical discovery. In this study, we performed a structure-activity relationship (SAR) study of DMEP derivatives as potential XooFtsZ inhibitors through introducing the structure-based virtual screening (SBVS) approach and various biochemical methods. Notably, prepared compound B2, a 4'-acyloxy DMEP analog, had a 50% inhibitory concentration of 159.4 µM for inhibition of recombinant XooFtsZ GTPase, which was lower than that of the parent DMEP (278.0 µM). Compound B2 potently inhibited Xoo growth in vitro (minimum inhibitory concentration 153 mg L-1) and had 54.9% and 48.4% curative and protective control efficiencies against rice blight in vivo. Moreover, compound B2 also showed low toxicity for non-target organisms, including rice plant and mammalian cell. Given these interesting results, we provide a novel strategy to discover and optimize promising bactericidal compounds for the management of plant bacterial diseases.


Subject(s)
Oryza , Xanthomonas , Anti-Bacterial Agents/chemistry , Bacterial Proteins/metabolism , Cell Division , Plant Diseases/microbiology , Plant Diseases/prevention & control , Podophyllotoxin/metabolism , Podophyllotoxin/pharmacology , Structure-Activity Relationship
17.
Angewandte Chemie ; 134(32), 2022.
Article in English | ProQuest Central | ID: covidwho-1971219

ABSTRACT

Der Nachweis von Nukleinsäuren spielt eine wichtige Rolle in der medizinischen Diagnostik, der Umweltüberwachung und der Lebensmittelsicherheit. In ihrem Forschungsartikel (e202203826) entwickelten Xue Gao, Yi Zhang und Mitarbeiter einen neuen Biosensor für den amplifikationsfreien Nukleinsäurenachweis, indem sie den trans‐Spaltungsmechanismus von Cas13a und ultrasensitive Graphen‐Feldeffekttransistoren (gFETs) nutzten. Die Abbildung zeigt die Cas13a‐vermittelte RNA‐trans‐Spaltung auf der gFET‐Oberfläche für die Sensorsignalübertragung.

18.
Nat Sci Sleep ; 14: 1175-1186, 2022.
Article in English | MEDLINE | ID: covidwho-1951798

ABSTRACT

Background: Gender differences in hippocampal and parahippocampal gyrus (HIP/PHG) volumes have been reported in sleep disorders. Therefore, this study investigated the moderating effect of gender on the relationship between chronic insomnia disorder (CID) and the HIP/PHG functional connectivity (FC) network. Methods: For this study, 110 patients diagnosed with CID (43 men and 67 women) and 60 matched good sleep control (GSC) (22 men and 38 women) were recruited. These participants underwent resting-state functional magnetic resonance imaging scans, after which a 2 × 2 (diagnosis × gender) analysis of variance was used to detect the main and interactive effect of insomnia and gender on their HIP/PHG FC networks. Results: Although the main effect of insomnia on the HIP FC network was observed in the bilateral cerebellar tonsil, superior frontal gyrus, and the medial orbitofrontal cortex, effects on the PHG FC network were observed in the bilateral HIP and amygdala. In contrast, the main effect of gender on the HIP FC network was observed in the right cerebellum posterior lobe, the dorsolateral prefrontal cortex (DLPFC), and the supplemental motor area. Of note, the interactive effect of both insomnia and gender was observed in FCs between the right HIP and the dorsal anterior cingulate cortex, and then between the right PHG and DLPFC. Moreover, the FC between the right PHG and left DLPFC was positively associated with anxiety scores in the female patients with CID. Conclusion: Our study identified that gender differences in brain connectivity existed between the HIP/PHG and executive control network in patients diagnosed with CID, these results will eventually extend our understanding of the important role that gender plays in the pathophysiology of CID.

20.
Angewandte Chemie ; n/a(n/a):e202209501, 2022.
Article in English | Wiley | ID: covidwho-1935651

ABSTRACT

Der Nachweis von Nukleinsäuren spielt eine wichtige Rolle in der medizinischen Diagnostik, der Umweltüberwachung und der Lebensmittelsicherheit. In ihrem Forschungsartikel (DOI: 10.1002/ange.202203826) entwickelten Xue Gao, Yi Zhang und Mitarbeiter einen neuen Biosensor für den amplifikationsfreien Nukleinsäurenachweis, indem sie den trans-Spaltungsmechanismus von Cas13a und ultrasensitive Graphen-Feldeffekttransistoren (gFETs) nutzten. Die Abbildung zeigt die Cas13a-vermittelte RNA-trans-Spaltung auf der gFET-Oberfläche für die Sensorsignalübertragung.

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