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1.
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences ; 49(2):270-274, 2020.
Article in Chinese | EuropePMC | ID: covidwho-1918770

ABSTRACT

目的 随访研究2019冠状病毒病(COVID-19)患者在达到出院标准后病毒核酸检测结果,为医疗机构对出院患者随访管理提供决策依据。 方法 对浙江大学医学院附属第四医院经治疗后已符合出院标准的COVID-19患者采取院内继续隔离观察7 d,再居家隔离4周,期间记录患者每日临床体征,定期送检痰液行病毒核酸检测,评估患者病情变化。 结果 已符合现有出院标准的7例患者中有3例在5~7 d后再次出现痰液病毒核酸检测阳性,持续阳性2~6 d后再次转阴;复查患者血常规、C反应蛋白、血生化以及胸部CT结果均无明显异常,也无明显临床症状。其余4例患者随访4周,痰液病毒核酸检测均为阴性,未出现COVID-19相关临床症状,实验室和影像学检查结果无异常。 结论 COVID-19患者按现有标准出院后复阳的比例较高,建议有条件的医疗机构对满足现行出院标准的患者继续院内隔离观察7 d并进行连续的病毒核酸监测。

2.
Journal of Cleaner Production ; : 132608, 2022.
Article in English | ScienceDirect | ID: covidwho-1882160

ABSTRACT

Despite the current slowdown in global economic growth due to the impact of COVID-19, the digital economy is still performing well. Under the background of double carbon, green innovation and intelligent production of manufacturing enterprises have become the general trend of sustainable development. It is particularly important to study the integration of digital technology into green innovation and production processes to improve the performance of digital green innovation and the competitiveness of enterprises. However, the integration of digital technology and green innovation from the perspective of knowledge management has not been fully introduced into current literatures. In this study, hierarchical regression and fsQCA approaches were used to empirically verify the adoption process of digital green innovation activities and the impact of digital green knowledge creation on digital green innovation performance (DGIP), and explores the moderating effects of digital green risk perception (DGRP) and digital green complexity perception (DGCC) through 429 questionnaires from Chinese manufacturing enterprises. In addition, knowledge search is divided into three dimensions: scientific digital green knowledge search (SDGKS), market digital green knowledge search (MDGKS) and supply chain digital green knowledge search (SCDGKS). The results show that: i) SDGKS promotes exploitative digital green knowledge creation (EDGKC). MDGKS has a positive impact on both utilizing digital green knowledge creation (ADGKC) and EDGKC. SCDGKS promotes EDGKC. ii) The relationship between SDGKS and EDGKC is only moderated by DGCC (positive). The relationship between MDGKS and EDGKC is only moderated by DGCC (negative). The relationship between SCDGKS and EDGKC is moderated by DGRP (negative) and DGCC (negative). iii) There is an inverted U-shaped relationship between ADGKC and DGIP. There is a U-shaped relationship between EDGKC and DGIP. The essence of this study is to help manufacturing enterprises find external partners to improve their digital green innovation performance through external knowledge search partner selection. The conclusion of this study has certain theoretical contribution to the clarification of the complex process of digital green innovation. This study provides a theoretical basis for enterprises to select knowledge search partners according to their own environment to carry out digital green innovation activities smoothly. This study has practical value for enterprises to improve competitiveness, better survival and development process under the current environment.

3.
Systems ; 10(2):46, 2022.
Article in English | ProQuest Central | ID: covidwho-1810205

ABSTRACT

At present, the polarization of online public opinion is becoming more frequent, and individuals actively participate in attitude interactions more and more frequently. Thus, online views have become the dominant force in current public opinion. However, the rapid fermentation of polarized public opinion makes it very easy for actual topic views to go to extremes. Significantly, negative information seriously affects the healthy development of the social opinion ecology. Therefore, it is beneficial to maintain national credibility, social peace, and stability by exploring the communication structure of online public opinions, analyzing the logical model of extreme public attitudes, and guiding the communication of public opinions in a timely and reasonable manner. Starting from the J–A model and BA network, this paper explores the specific attributes of individuals and opinion network nodes. By incorporating parameters such as individual conformity and the strength of individual online relationships, we established a model of online group attitude polarization, then conducted simulation experiments on the phenomenon of online opinion polarization. Through simulations, we found that individual conformity and the difference in environmental attitude greatly influence the direction of opinion polarization events. In addition, crowd mentality makes individuals spontaneously choose the side of a particular, extreme view, which makes it easier for polarization to form and reach its peak.

4.
EuropePMC; 2020.
Preprint in English | EuropePMC | ID: ppcovidwho-324796

ABSTRACT

In China, mothers with confirmed or suspected COVID-19 pneumonia are recommended to stop breastfeeding. However, the evidence to support this guidance is lacking. This report analyzes the case of a mother who persisted breastfeeding her baby when both were diagnosed with confirmed COVID-19 pneumonia. SARS-CoV-2 nucleic acid was not detected in the breast milk, and antibodies against SARS-CoV-2 were present in detected in the mother's serum and milk. There was no evidence of mother-to-child transmission of the virus through breastfeeding. This case report demonstrates that breastfeeding may even be useful for improving the recovery of the infant without exacerbating disease severity.

5.
J Clin Med ; 11(1)2021 Dec 22.
Article in English | MEDLINE | ID: covidwho-1580658

ABSTRACT

Extracorporeal membrane oxygenation (ECMO) is potentially lifesaving for patients with acute respiratory distress syndrome (ARDS) but may be accompanied by serious adverse events, including intracranial hemorrhage (ICRH). We hypothesized that ICRH occurs more frequently in patients with COVID-19 than in patients with ARDS of other etiologies. We performed a single-center retrospective analysis of adult patients treated with venovenous (vv-) ECMO for ARDS between January 2011 and April 2021. Patients were included if they had received a cranial computed tomography (cCT) scan during vv-ECMO support or within 72 h after ECMO removal. Cox regression analysis was used to identify factors associated with ICRH. During the study period, we identified 204 patients with vv-ECMO for ARDS, for whom a cCT scan was available. We observed ICRH in 35.4% (n = 17/48) of patients with COVID-19 and in 16.7% (n = 26/156) of patients with ARDS attributable to factors other than COVID-19. COVID-19 (HR: 2.945; 95%; CI: 1.079-8.038; p = 0.035) and carboxyhemoglobin (HR: 0.330; 95%; CI: 0.135-0.806; p = 0.015) were associated with ICRH during vv-ECMO. In patients receiving vv-ECMO, the incidence of ICRH is doubled in patients with COVID-19 compared to patients suffering from ARDS attributable to other causes. More studies on the association between COVID-19 and ICRH during vv-ECMO are urgently needed to identify risk patterns and targets for potential therapeutic interventions.

6.
Nat Struct Mol Biol ; 28(7): 614-625, 2021 07.
Article in English | MEDLINE | ID: covidwho-1550333

ABSTRACT

p97 processes ubiquitinated substrates and plays a central role in cellular protein homeostasis. Here, we report a series of cryo-EM structures of the substrate-engaged human p97 complex with resolutions ranging from 2.9 to 3.8 Å that captured 'power-stroke'-like motions of both the D1 and D2 ATPase rings of p97. A key feature of these structures is the critical conformational changes of the intersubunit signaling (ISS) motifs, which tighten the binding of nucleotides and neighboring subunits and contribute to the spiral staircase conformation of the D1 and D2 rings. In addition, we determined the cryo-EM structure of human p97 in complex with NMS-873, a potent p97 inhibitor, at a resolution of 2.4 Å. The structures showed that NMS-873 binds at a cryptic groove in the D2 domain and interacts with the ISS motif, preventing its conformational change and thus blocking substrate translocation allosterically.


Subject(s)
Adenosine Triphosphate/chemistry , Protein Folding , Proteostasis/physiology , Signal Transduction/physiology , Valosin Containing Protein/metabolism , Acetanilides/pharmacology , Animals , Benzothiazoles/pharmacology , Cryoelectron Microscopy , Endoplasmic Reticulum/metabolism , Endoplasmic Reticulum-Associated Degradation/physiology , Humans , Intracellular Signaling Peptides and Proteins/metabolism , Nuclear Proteins/metabolism , Proteasome Endopeptidase Complex/metabolism , Protein Conformation , Ubiquitinated Proteins/metabolism , Valosin Containing Protein/antagonists & inhibitors
7.
J Med Virol ; 92(10): 1938-1947, 2020 10.
Article in English | MEDLINE | ID: covidwho-967766

ABSTRACT

BACKGROUND: With the effective prevention and control of COVID-19 in China, the number of cured cases has increased significantly. Further monitoring of the disease prognosis and effective control of the "relapse" of the epidemic has become the next focus of work. This study analysed the clinical prognosis of discharged COVID-19 patients by monitoring their SAR-CoV-2 nucleic acid status, which provided a theoretical basis for medical institutions to formulate discharge standards and follow-up management for COVID-19 patients. METHODS: We included 13 discharged COVID-19 patients who were quarantined for 4 weeks at home. The patient's daily clinical signs were recorded and sputum and faecal specimens were regularly sent for detection of SARS-CoV-2 nucleic acid. RESULTS: The time between initial symptoms and meeting discharge criteria was 18 to 44 days with an average of 25 ± 6 days. The faecal samples of two patients still tested positive after meeting the discharge criteria and the sputum samples of four patients returned positive 5 to 14 days after discharge. The rate of the recurring positive test result in samples from the respiratory system was 31% (4/13). CONCLUSION: Under the present discharge criteria, the high presence of SARS-CoV-2 nucleic acid in faecal and respiratory samples of discharged COVID-19 patients indicates potential infectivity. Therefore, we suggest that faecal virus nucleic acid should be tested as a routine monitoring index for COVID-19 and a negative result be added to the criteria. Simultaneously, we should strengthen the regular follow-up of discharged patients with continuous monitoring of the recurrence of viral nucleic acid.


Subject(s)
COVID-19/diagnosis , Feces/virology , Patient Discharge , SARS-CoV-2/isolation & purification , Sputum/virology , Adult , Aged , China , Female , Humans , Infant , Male , Middle Aged , RNA, Viral/isolation & purification , Young Adult
8.
Int Breastfeed J ; 15(1): 68, 2020 08 06.
Article in English | MEDLINE | ID: covidwho-696998

ABSTRACT

BACKGROUND: In China, mothers with confirmed or suspected COVID-19 pneumonia are recommended to stop breastfeeding. However, the evidence to support this guidance is lacking. There have been relatively few cases reported about direct breastfeeding an infant by a mother with SARS-CoV-2 pneumonia. Therefore, it is necessary to assess the safety of breastfeeding and the possible protective effects of breast milk on infants. CASE PRESENTATION: This report analyzes the case of a mother who continued breastfeeding her 13 month-old child when both were diagnosed with confirmed COVID-19 pneumonia. We describe the clinical presentation, diagnosis, treatment, and outcome. The presence of SARS-CoV-2 nucleic acid was determined in maternal serum, breast milk, nasopharyngeal (NP) swabs and feces, and in infant serum, NP swabs and feces. IgM and IgG antibodies against SARS-CoV-2 were assessed in maternal serum and breast milk and in infant serum. SARS-CoV-2 nucleic acid was not detected in the breast milk, and antibodies against SARS-CoV-2 were detected in the mother's serum and milk. CONCLUSIONS: The present case further confirms that the possibility of mother-to-child transmission about SARS-CoV-2 via breast milk alone was very small, and breast milk is safe for direct feeding of infants.


Subject(s)
Antibodies, Viral/analysis , Betacoronavirus/isolation & purification , Breast Feeding , Coronavirus Infections/transmission , Milk, Human/virology , Pneumonia, Viral/transmission , COVID-19 , Coronavirus Infections/prevention & control , Female , Humans , Infant , Infectious Disease Transmission, Vertical , Pandemics/prevention & control , Pneumonia, Viral/prevention & control , SARS-CoV-2
10.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 2020.
Article | WHO COVID | ID: covidwho-238993

ABSTRACT

OBJECTIVE: To investigate the clinical outcome of patients with moderate type of coronavirus disease 2019 (COVID-19) after discharge by retesting viral nucleic acid. METHODS: Seven patients with moderate COVID-19 met the discharge criteria enacted by National Health Commission were quarantined in hospital for 7 days, then continuously quarantined at home for 4 weeks after discharged. During the quarantined period, the symptoms and signs were documented, and sputum or nasal swab and feces samples were collected to test SARS-CoV-2 nucleic acid by RT-PCR method. RESULTS: There was no symptoms and signs during the quarantine period in all 7 patients. However, respiratory swabs from 3 patients were confirmed positive of SARS-CoV-2 nucleic acid at 5 to 7 days after they met the discharge criteria. CONCLUSIONS: There is a relatively high incidence of positive viral nucleic acid in patients met the discharge criteria, and it is suggested that patients met the current discharge criteria should be quarantined in hospital for another 7 days and the follow-up viral testing is necessary.

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