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1.
Front Endocrinol (Lausanne) ; 13: 753267, 2022.
Article in English | MEDLINE | ID: covidwho-1902944

ABSTRACT

An extreme strain has been placed on healthcare facilities in the COVID-19 era. Initial stage of the pandemic, national and international societies for reproductive medicine suggested the suspension of new IVF treatments and non-essential cryopreservation of gametes. Accordingly, the demands of cryopreservation of semen with COVID-19 patients also was suspended by some of cryobanks to protect staff and patients from unnecessary viral exposure at the acute stage. However, the pandemic may stay with us longer than expected. In addition, there will be some male COVID-19 patients with cancer or critically illness who needs to cryopreserve their semen before medical treatments, otherwise they might loss the chance of getting their own offspring. In this document, we summarize available evidence to deepen and expand awareness of feasibility of sperm cryopreservation and propose some suggestions to help cryobanks carry out sperm preservation procedure for COVID-19 male patients.


Subject(s)
COVID-19 , Semen Preservation , COVID-19/epidemiology , COVID-19/therapy , Cryopreservation/methods , Humans , Male , Pandemics , Spermatozoa
2.
Natural Product Research and Development ; 33(11):1957-1968, 2021.
Article in Chinese | CAB Abstracts | ID: covidwho-1771917

ABSTRACT

To analyze the possible mechanism of the intervention of absorbed components of Lianhua Qingwen Capsule on COVID-19 from the perspective of cytokine storm based on network pharmacology and molecular docking. Through literature mining, the components of Lianhua Qingwen capsules into blood were determined, and the related action targets of the components were searched by TCMSP and BATMAN-TCM databases. The related targets of COVID-19 and cytokine storm were screened by GeneCards, OMIM and Drugbank databases. The protein interaction PPI network was constructed through String database. DAVID database was used for GO analysis and KEGG pathway analysis. Cytoscape 3.8.0 software was used to build the network diagram. Molecular docking was performed by AutoDock software.In addition, multiple organ tissue injury, immune injury and SARS related targets were retrieved and intersected with cytokine storm.A total of 17 absorbed components of Lianhua Qingwen Capsules were collected, 237 corresponding targets and 47 targets intersected with diseases obtained, and 22 core targets screened out.GO analysis and KEGG pathway analysis presented 174 entries and 83 entries respectively (P < 0.01). The molecular docking results showed that the compounds of Emodin, Formononetin, Rutin, Gallic acid, Liquiritigenin had good binding ability with the core target of AKT1, IL-6, TP53, JUN, TNF. The proportion of intersecting target of Lianhua Qingwen Capsule and multiple organ tissue injury and immune injury was 1.6%-2.0%, and the proportion of intersecting target and SARS was 4.3%. Absorbed components of Lianhua Qingwen Capsule could effectively prevent and treat COVID-19 by intervening cytokine storm through multi-component, multi-target and multi-pathway synergistic action, and its mechanism may be related to antigen-scavenging, immune-regulating and tissue and organ protection.

3.
Cell ; 185(10): 1728-1744.e16, 2022 05 12.
Article in English | MEDLINE | ID: covidwho-1767964

ABSTRACT

As the emerging variants of SARS-CoV-2 continue to drive the worldwide pandemic, there is a constant demand for vaccines that offer more effective and broad-spectrum protection. Here, we report a circular RNA (circRNA) vaccine that elicited potent neutralizing antibodies and T cell responses by expressing the trimeric RBD of the spike protein, providing robust protection against SARS-CoV-2 in both mice and rhesus macaques. Notably, the circRNA vaccine enabled higher and more durable antigen production than the 1mΨ-modified mRNA vaccine and elicited a higher proportion of neutralizing antibodies and distinct Th1-skewed immune responses. Importantly, we found that the circRNARBD-Omicron vaccine induced effective neutralizing antibodies against the Omicron but not the Delta variant. In contrast, the circRNARBD-Delta vaccine protected against both Delta and Omicron or functioned as a booster after two doses of either native- or Delta-specific vaccination, making it a favorable choice against the current variants of concern (VOCs) of SARS-CoV-2.


Subject(s)
COVID-19 , SARS-CoV-2 , Animals , Antibodies, Neutralizing , Antibodies, Viral , COVID-19/prevention & control , COVID-19 Vaccines , Humans , Macaca mulatta , Mice , RNA, Circular/genetics , SARS-CoV-2/genetics , Vaccines, Synthetic/genetics , mRNA Vaccines
4.
Drug Evaluation Research ; 43(9):1663-1672, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-1374636

ABSTRACT

Objective: To explore the active ingredients, targets, pathways and molecular mechanism of Xinjia Xiangru Decoction in the possible treatment of new coronavirus pneumonia (COVID-19) through network pharmacology and molecular docking technology.

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