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1.
Curr Med Sci ; 41(2): 318-322, 2021 Apr.
Article in English | MEDLINE | ID: covidwho-1193161

ABSTRACT

Serology tests for viral antibodies provide an important tool to support nucleic acid testing for diagnosis of the novel coronavirus disease 2019 (COVID-19) and is useful for documenting previous exposures to SARS-CoV-2, the etiological agent of COVID-19. The sensitivities of the chemiluminescent SARS-CoV-2 IgG/IgM immunoassay were assessed by using serum samples collected from 728 patients testing positive for SARS-CoV-2 RNA. The specificity was evaluated on a panel of 60 serum samples from non-COVID-19 patients with high levels of rheumatoid factor, antinuclear antibody, or antibodies against Epstein-Barr virus (EBV), cytomegalovirus (CMV), mycoplasma pneumonia, human respiratory syncytial virus (RSV), adenovirus, influenza A or influenza B. The imprecision and interference were assessed by adopting the Clinical and Laboratory Standards Institute (CLSI) EP15-A2 and EP7-A2, respectively. Sensitivities between 1 and 65 days after onset of symptoms were 94.4% and 78.7%, for IgG and IgM test, respectively. The sensitivity increased with the time after symptom onset, and rose to the top on the 22nd to 28th days. The total imprecision (CVs) was less than 6.0% for IgG and less than 6.5% for IgM. Limited cross-reactions with antibodies against EBV, CMV, mycoplasma pneumonia, human RSV, adenovirus, influenza A or influenza B were found. These data suggested the chemiluminescent SARS-CoV-2 IgG and IgM, assay with reliable utility and sensitivity, could be used for rapid screening and retrospective surveillance of COVID-19.


Subject(s)
Antibodies, Viral/blood , /blood , /isolation & purification , Adolescent , Adult , Aged , Aged, 80 and over , /virology , Female , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Luminescent Measurements/methods , Male , Middle Aged , RNA, Viral/blood , Retrospective Studies , Young Adult
2.
Nat Med ; 27(4): 668-676, 2021 04.
Article in English | MEDLINE | ID: covidwho-1174686

ABSTRACT

Drug repurposing provides a rapid approach to meet the urgent need for therapeutics to address COVID-19. To identify therapeutic targets relevant to COVID-19, we conducted Mendelian randomization analyses, deriving genetic instruments based on transcriptomic and proteomic data for 1,263 actionable proteins that are targeted by approved drugs or in clinical phase of drug development. Using summary statistics from the Host Genetics Initiative and the Million Veteran Program, we studied 7,554 patients hospitalized with COVID-19 and >1 million controls. We found significant Mendelian randomization results for three proteins (ACE2, P = 1.6 × 10-6; IFNAR2, P = 9.8 × 10-11 and IL-10RB, P = 2.3 × 10-14) using cis-expression quantitative trait loci genetic instruments that also had strong evidence for colocalization with COVID-19 hospitalization. To disentangle the shared expression quantitative trait loci signal for IL10RB and IFNAR2, we conducted phenome-wide association scans and pathway enrichment analysis, which suggested that IFNAR2 is more likely to play a role in COVID-19 hospitalization. Our findings prioritize trials of drugs targeting IFNAR2 and ACE2 for early management of COVID-19.


Subject(s)
/genetics , Drug Repositioning , Mendelian Randomization Analysis/methods , /genetics , /drug therapy , Genome-Wide Association Study , Humans , Interleukin-10 Receptor beta Subunit/genetics , Interleukin-10 Receptor beta Subunit/physiology , Quantitative Trait Loci , Receptor, Interferon alpha-beta/genetics , Receptor, Interferon alpha-beta/physiology
3.
Biomolecules ; 11(2)2021 02 22.
Article in English | MEDLINE | ID: covidwho-1090376

ABSTRACT

Peptide drugs, especially food-derived peptides, have a variety of functional activities including antiviral and may also have a therapeutic effect on COVID-19. In this study, comparing with the reported drugs, 79 peptides were found to bind to the key targets of COVID-19 virus with higher non-covalent interaction, while among them, six peptides showed high non-covalent interactions with the three targets, which may inhibit the COVID-19 virus. In the simulation, peptides of nine to 10 amino acids with a hydrophilic amino acid and acidic amino acid in the middle and aromatic amino acids on the side showed higher binding to angiotensin-converting enzyme 2 (ACE2). Peptides of five to six amino acids with a basic amnio acid in the head, acidic amnio acid in the neck, hydrophobicity group in the middle, and basic amino acids in the tail showed higher binding to COVID-19 virus main protease (Mpro), while those with basic amino acids and acidic amino acids in the two sides and aromatic amino acids in the middle might have stronger interaction with COVID-19 virus RNA-dependent RNA polymerase (RdRp).


Subject(s)
/drug therapy , Drug Discovery , Peptides/chemistry , Peptides/pharmacology , /drug effects , /chemistry , /metabolism , Drug Design , Functional Food , Humans , Molecular Docking Simulation , Molecular Dynamics Simulation , Molecular Targeted Therapy , /metabolism , Viral Matrix Proteins/chemistry , Viral Matrix Proteins/metabolism
4.
Preprint | SciFinder | ID: ppcovidwho-4971

ABSTRACT

Objective To observe the effect of peroxyacetic acid in the laboratoiy disinfection of SARS-CoV-2 nucleic acid detection, and to provide a reference for laboratoiy prevention and control of SARS-CoV-2 detection Methods Using gene detection technol the effectiveness of compound peroxyacetic acid disinfectant in sterilizing laboratoiy for SARS-CoV-2 was evaluated Results The compound peroxyacetic acid contained about 2 5 g/L of peroxyacetic acid and 60 g/L of hydrogen peroxide The stock solution was used in an aerosol elec sprayer at a dosage of 22 mL/m3 in a specimen preparation room of laboratoiy for SARS-CoV-2 nucleic acid detection Spraying disinfection was conducted and the room was sealed for 60 min The laboratory had been spray - sterilized at the end of each work for 30 consecutive days, and all collected laboratoiy environmental specimens have not detected the pos SARS-CoV-2 nucleic acid and there had never been any incident of staff infection with SARS-CoV-2 Conclusion The results prove that spray disinfection of laboratoiy for SARS-CoV-2 detection with the compound peroxyacetic acid can effectively kill the SARS-CoV-2 in laboratoiy environment and can effectively protect the safety of staff

5.
Preprint | SciFinder | ID: ppcovidwho-4743

ABSTRACT

A review of research progress of COVID-19 from perspectives of pathogenesis, epidemiol , clin symptoms, CT scam imaging, and treatment with medicines

6.
Emerg Microbes Infect ; : 1-29, 2020 Nov 24.
Article in English | MEDLINE | ID: covidwho-977352

ABSTRACT

In the face of COVID-19 pandemic caused by the newly emerged SARS-CoV-2, an inactivated, Vero cell-based, whole virion vaccine candidate has been developed and entered into phase III clinical trials within six months. Biochemical and immunogenic characterization of structural proteins and their post-translational modifications in virions, the end-products of the vaccine candidate, would be essential for the quality control and process development of vaccine products and for studying the immunogenicity and pathogenesis of SARS-CoV-2. By using a panel of rabbit antisera against virions and five structural proteins together with a convalescent serum, the spike (S) glycoprotein was shown to be N-linked glycosylated, PNGase F-sensitive, endoglycosidase H-resistant and cleaved by Furin-like proteases into S1 and S2 subunits. The full-length S and S1/S2 subunits could form homodimers/trimers. The membrane (M) protein was partially N-linked glycosylated; the accessory protein 3a existed in three different forms, indicative of cleavage and dimerization. Furthermore, analysis of the antigenicity of these proteins and their post-translationally modified forms demonstrated that S protein induced the strongest antibody response in both convalescent and immunized animal sera. Interestingly, immunization with the inactivated vaccine did not elicit antibody response against the S2 subunit, whereas strong antibody response against both S1 and S2 subunits was detected in the convalescent serum. Moreover, vaccination stimulated stronger antibody response against S multimers than did the natural infection. This study revealed that the native S glycoprotein stimulated neutralizing antibodies, while bacterially-expressed S fragments did not. The study on S modifications would facilitate design of S-based anti-SARS-CoV-2 vaccines.

7.
Preprint | CAplus | ID: ppcovidwho-2043

ABSTRACT

A review The outbreak of a new type of coronavirus (SARS-CoV-2) pneumonia has been spreading all over the world, drawing worldwide attention However, the origin of the novel coronavirus has not been determined yet Traditional Chinese medicine played an important role in treating SARS-CoV-2 infection The state attaches great importance to the role of TCM in the prevention and treatment of SARS-CoV-2 infection, and strengthens the early intervention of TCM in medical treatment Traditional Chinese medicine has its own characteristics and advantages in the prevention and treatment of viral pneumonia, with better clin efficacy and fewer side effects In addition to eliminating or alleviating clin symptoms, it can also regulate the immune function of patients and prevent the overreaction of the body caused by viruses This article reviews the research on the immunomodulatory action of traditional Chinese medicine in the prevention and treatment of viral pneumonia, hoping to provide some reference for the clin medication and later basic research of viral pneumonia including SARS-CoV-2

8.
Front Cell Dev Biol ; 8: 589, 2020.
Article in English | MEDLINE | ID: covidwho-689162

ABSTRACT

COVID-19, a novel coronavirus pneumonia (named by the World Health Organization, WHO), has spread widely since the end of 2019. Research on synthetic drugs and vaccines has become a focus of attention in China and other countries, as such approaches are regarded as key tools for disease prevention and control; however, the development of these therapeutics will take months, or even years. Under such circumstances, development of coronavirus specific therapeutics is urgent. For this specific indication, the rapid performance of natural products, such as plant compounds, herbal extracts, and traditional Chinese medicine, could contribute as alternative measures. Recent investigations have provided evidence that these natural products are potential candidates for development as therapeutic agents against the virus that causes COVID-19, 2019-nCoV. Targeting the structural proteins or cellular receptors of 2019-nCoV, including coronavirus chymotrypsin-like (3CLpro or Mpro), helicase (nsP13), S protein, and human angiotensin converting enzyme 2 (ACE2), holds promise for preventing infection. In this review, we summarize some representative natural products and their active components that have potential anti-2019-nCoV effects. We focus on the basic structural elements of 2019-nCoV, its main mechanisms of action, and the feasibility and potential of products to inhibit the novel coronavirus. In addition, the relative advantages, additional functions, and precautions that should be used with typical natural products are also discussed. The aim is to make the case that natural products could be a valuable pool for the development of active compounds for treating 2019-nCoV infection, which may contribute to mitigation of the spread of the pandemic.

9.
Nonlinear Dyn ; : 1-7, 2020 Jun 22.
Article in English | MEDLINE | ID: covidwho-610315

ABSTRACT

Recently, COVID-19 has attracted a lot of attention of researchers from different fields. Wearing masks is a frequently adopted precautionary measure. In this paper, we investigate the effect of behavior of wearing masks on epidemic dynamics in the context of COVID-19. At each time, every susceptible individual chooses whether to wear a mask or not in the next time step, which depends on an evaluation of the potential costs and perceived risk of infection. When the cost of infection is high, the majority of the population choose to wear masks, where global awareness plays a significant role. However, if the mask source is limited, global awareness may give rise to a negative result. In this case, more mask source should be allocated to the individuals with high risk of infection.

10.
Chin J Traumatol ; 23(4): 196-201, 2020 Aug.
Article in English | MEDLINE | ID: covidwho-601868

ABSTRACT

Outbreak of COVID-19 is ongoing all over the world. Spine trauma is one of the most common types of trauma and will probably be encountered during the fight against COVID-19 and resumption of work and production. Patients with unstable spine fractures or continuous deterioration of neurological function require emergency surgery. The COVID-19 epidemic has brought tremendous challenges to the diagnosis and treatment of such patients. To coordinate the diagnosis and treatment of infectious disease prevention and spine trauma so as to formulate a rigorous diagnosis and treatment plan and to reduce the disability and mortality of the disease, multidisciplinary collaboration is needed. This expert consensus is formulated in order to (1) prevent and control the epidemic, (2) diagnose and treat patients with spine trauma reasonably, and (3) reduce the risk of cross-infection between patients and medical personnel during the treatment.


Subject(s)
Betacoronavirus , Coronavirus Infections/epidemiology , Pneumonia, Viral/epidemiology , Practice Guidelines as Topic , Spinal Injuries/diagnosis , Spinal Injuries/therapy , Coronavirus Infections/prevention & control , Cross Infection/prevention & control , Emergency Service, Hospital , Humans , Pandemics/prevention & control , Patient Care Team , Pneumonia, Viral/prevention & control , Transportation of Patients
11.
JMIR Med Inform ; 8(6): e19515, 2020 Jun 08.
Article in English | MEDLINE | ID: covidwho-574511

ABSTRACT

BACKGROUND: The coronavirus disease (COVID-19) epidemic poses an enormous challenge to the global health system, and governments have taken active preventive and control measures. The health informatics community in China has actively taken action to leverage health information technologies for epidemic monitoring, detection, early warning, prevention and control, and other tasks. OBJECTIVE: The aim of this study was to develop a technical framework to respond to the COVID-19 epidemic from a health informatics perspective. METHODS: In this study, we collected health information technology-related information to understand the actions taken by the health informatics community in China during the COVID-19 outbreak and developed a health information technology framework for epidemic response based on health information technology-related measures and methods. RESULTS: Based on the framework, we review specific health information technology practices for managing the outbreak in China, describe the highlights of their application in detail, and discuss critical issues to consider when using health information technology. Technologies employed include mobile and web-based services such as Internet hospitals and Wechat, big data analyses (including digital contact tracing through QR codes or epidemic prediction), cloud computing, Internet of things, Artificial Intelligence (including the use of drones, robots, and intelligent diagnoses), 5G telemedicine, and clinical information systems to facilitate clinical management for COVID-19. CONCLUSIONS: Practical experience in China shows that health information technologies play a pivotal role in responding to the COVID-19 epidemic.

12.
Nan Fang Yi Ke Da Xue Xue Bao ; 2020.
Article | WHO COVID | ID: covidwho-212502

ABSTRACT

OBJECTIVE: To investigate the psychological status and sleep quality of nursing interns in collective isolation during the outbreak of coronavirus disease 2019 (COVID-19) and provide evidence for adequate interventions METHODS: We surveyed a total of 95 nursing interns who were isolated collectively in a general teaching hospital in Guangzhou using a self-designed questionnaire, which consisted of a basic information form, self-rating anxiety scale (SAS), self-rating depression scale (SDS) and Pittsburgh Sleep Quality Index (PSQI) Descriptive analysis, single factor analysis and correlation analysis were used to analyze the current status of the interns' psychology and sleep quality, the potential factors affecting their psychology and sleep quality, and the correlation between their psychological status and sleep quality RESULTS: The surveyed interns had SAS, SDS and PSQI score of 37 79+/-6 59, 43 98+/-9 74 and 5 20+/-3 14, respectively, which were significant higher than the national norms in China (P < 0 05) Correlation analysis indicated that both anxiety and depression were positively correlated with the sleep quality score (r=0 508 and 0 546, respectively) Univariate analysis showed that the major factors affecting the psychological status and sleep quality of the interns during collective isolation included recent contact with persons from the affected area before isolation and the onset of fever during the isolation CONCLUSIONS: These interns showed relatively high levels of anxiety and depression during the collective isolation to affect their sleep quality, and interventions should be timely administered to improve their mental health and sleep quality

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