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European Respiratory Journal Conference: European Respiratory Society International Congress, ERS ; 60(Supplement 66), 2022.
Article in English | EMBASE | ID: covidwho-2269375
Acta Veterinaria et Zootechnica Sinica ; 53(7):2260-2267, 2022.
Article in Chinese | EMBASE | ID: covidwho-2245652
Methods in Molecular Biology ; 2591:269-282, 2023.
Article in English | Scopus | ID: covidwho-2244456
Human Gene Therapy Methods ; 33(23-24):A158, 2022.
Article in English | EMBASE | ID: covidwho-2188084
Biochimica et Biophysica Acta - Bioenergetics ; Conference: EBEC2022, 2022.
Article in English | EMBASE | ID: covidwho-2176722
Front Cell Infect Microbiol ; 12: 960938, 2022.
Article in English | MEDLINE | ID: covidwho-2154694


Coronavirus disease 2019 (COVID-19) is an extremely contagious illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Early disease recognition of COVID-19 is crucial not only for prompt diagnosis and treatment of the patients, but also for effective public health surveillance and response. The reverse transcription-polymerase chain reaction (RT-PCR) is the most common method for the detection of SARS-CoV-2 viral mRNA and is regarded as the gold standard test for COVID-19. However, this test and those for antibodies (IgM and IgG) and antigens have certain limitations (e.g., by yielding false-negative and false-positive results). We have developed an RNA fluorescence in situ hybridization (FISH) method for high-sensitivity detection of SARS-CoV-2 mRNAs in HEK 293T cell cultures as a model. After transfection of HEK 293T cells with plasmids, Spike (S)/envelope (E) proteins and their mRNAs were clearly detected inside the cells. In addition, hybridization time could be reduced to 2 hours for faster detection when probe concentration was increased. Our approach might thus significantly improve the sensitivity and specificity of SARS-CoV-2 detection and be widely applied for the high-sensitivity single-molecular detection of other RNA viruses (e.g., Middle East respiratory syndrome coronavirus (MERS-CoV), Hepatitis A virus, all influenza viruses, and human immunodeficiency virus (HIV)) in various types of samples including tissue, body fluid, blood, and water. RNA FISH can also be utilized for the detection of DNA viruses (e.g., Monkeypox virus, human papillomavirus (HPV), and cytomegalovirus (CMV)) by detection of their mRNAs inside cells or body fluid.

COVID-19 , SARS-CoV-2 , Humans , SARS-CoV-2/genetics , COVID-19 Testing , Clinical Laboratory Techniques/methods , RNA, Messenger/genetics , In Situ Hybridization, Fluorescence , HEK293 Cells , Immunoglobulin M , Immunoglobulin G , Water
Topics in Antiviral Medicine ; 30(1 SUPPL):182-183, 2022.
Article in English | EMBASE | ID: covidwho-1880240