ABSTRACT
Due to various policies measures to curtail the virus, one of which was lockdown, the occurrence of COVID-19 had a negative impact on the nation's economy, especially the rural smallholder farmers. The study looked into how Covid-19 lockdowns affected Nigerian vegetable production. Descriptive statistics, budgetary approaches, multiple regression analysis, and a Likert scale were all used for the analysis. The findings show that most of vegetable growers in the research region were married women (88.33%). About 70% of respondents have completed at least their primary education. The multiple regression analysis results disclosed that number of extensions contact, farm size, market access and transportation cost were significantly affected the level of vegetable production with positive sign while household size had a negative effect on the vegetable production. The findings also showed that after the COVID-19 lockdown, vegetable production was extremely profitable in the study area, with an average gross margin of 82,836.04 that was statistically higher than the 60,709.97 gross margin generated from vegetable production during COVID-19 and the 71,234.91 gross margin generated prior to COVID-19. The constraints faced by vegetable farmers during the covid - 19 restrictions include: high cost of transportation, price fluctuation, and high cost of input, perishability of the produce and lack of improved seeds. It was concluded that Covid 19 pandemic has greater effect on vegetable production in the study areas.
ABSTRACT
Oilseeds are grown mainly for the extraction of vegetable oils and for its by-products needed in livestock feed and in other industrial uses. The oils obtained from them are becoming a staple food used in daily cooking in several countries, and as a result the world demand is constantly increasing. This situation, combined with the exponential increase in the world population and other cyclical factors, is leading to a surge pricing, especially in importing countries. This increase in prices is fueled by soaring oil prices and disruption in supplies following Covid-19 pandemic and geopolitical tensions in the Black Sea. Morocco is directly impacted by these fluctuations given that the country imports almost its total needs in vegetable oils, oilseeds and meals. The high dependence on imported vegetable oils and oilseed products has a detrimental effect on the economy of Morocco and weighs heavily on the country's trade balance. Considering their increasingly important role in society, the development of a local oilseed sector to reduce Morocco's dependence on imports and cope with the vagaries of global markets has never been more topical in the current context of sustainable agriculture and food sovereignty.
ABSTRACT
The novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began in Wuhan, China, in December 2019 and quickly spread across the worldwide. It becomes a global pandemic and risk to the healthcare system of almost every nation around the world. In this study thirty natural compounds of 19 Indian herbal plants were used to analyze their binding with eight proteins associated with COVID -19. Based on the molecular docking as well as ADMET analysis, isovitexin, glycyrrhizin, sitosterol, and piperine were identified as potential herbal medicine candidates. On comparing the binding affinity with Ivermectin, we have found that the inhibition potentials of the Trigonella foenum-graecum (fenugreek), Glycyrrhiza glabra (licorice), Tinospora cordifolia (giloy) and Piper nigrum (black pepper) are very promising with no side-effects.
ABSTRACT
Swine acute diarrhea syndrome coronavirus (SADS-CoV), a newly discovered enteric coronavirus, is the etiological agent that causes severe clinical diarrhea and intestinal pathological damage in piglets. In this study, Vero E6 and IPI-2I cells were pretreated with different concentrations of glycyrrhizin (GLY) for 2 hours, and then infected with different concentrations of SADSCoV, aiming to investigate the inhibitory effect of GLY on SADS-CoV. Western blot and TCID50 results revealed a significantly decreased N protein expression and viral titer, indicating that GLY can inhibit the infection of SADS-CoV. Vero E6 and IPI-2I cells were pretreated with different concentrations of GLY for 2 hours and infected with SADS-CoV. Western blot results showed that when the concentration of GLY was 0.8 mmol/L, the expression of N protein decreased significantly, indicating that GLY inhibited the invasion of the virus. At first, cells were treated with 0.4 mmol/L GLY, and cell samples were collected at 2 hours, 6 hours and 12 hours after being infected with SADS-CoV for analysis, and the expression of N protein were found to be significantly reduced at all points, indicating that GLY had a significant inhibitory effect on the replication of the virus. GLY is a competitive inhibitor of high mobility group box 1 (HMGB1), and the receptors of HMGB1 mainly include TLR4 and RAGE. Based on this fact, the mutant plasmid at the key sites of HMGB1 (C45S, C106S, C45/106S) and the siRNA of the RAGE receptor were transfected to Vero E6 cells and infected with SADS-CoV, and the cell supernatant and samples were harvested. The western blot and TCID50 results showed that the expression of N protein and the virus titer were decreased, suggesting that GLY exerts its function by affecting the binding of HMGB1/TLR4/RAGE during SADS-CoV infection. To further explore the signaling pathway through which GLY functions, Vero E6 and IPI-2I cells were inoculated with SADS-CoV, and cell samples were harvested, western blot was used to detect the changes of MAPK proteins. The results showed that the protein expression levels of p-p38, p-JNK and p-ERK were up-regulated in the early and late stages, indicating that the MAPK pathway was activated by SADS-CoV infection. Vero E6 and IPI-2I were pretreated with different concentrations of GLY and TLR4 inhibitor TAK for 2 hours and infected with SADS-CoV. Protein samples were harvested and analysed by western blot which showed a decreased p-JNK and N proteins, while other proteins showed no significant changes. These results indicated that GLY and TAK regulated the phosphorylation of JNK but did not regulate the phosphorylation of p38 and ERK. Also, Vero E6 cells were treated with HMGB1 antibody, the siRNA of HMGB1 and HMGB1 mutants plasmid, and infected with SADS-CoV. Protein samples were harvested, western blot results showed that phosphorylation of JNK decreased, indicating that HMGB1 affected JNK phosphorylation. Finally, Vero E6 and IPI-2I cells were pretreated with different concentrations of JNK inhibitor SP600125 to infect SADS-CoV, western blot, TCID50 and IFA results showed that the expression of N protein and virus titer, as well as virus replication were reduced, indicating that SP600125 inhibited virus replication. In conclusion, our results revealed that GLY can inhibit in vitro replication of SADS- CoV, mainly through the HMGB1/TLR4/JNK signaling pathway. The discovery of this pathway provides theoretical support for the research of novel anti-SADS-CoV drugs.