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1.
Transbound Emerg Dis ; 2022 Oct 17.
Article in English | MEDLINE | ID: covidwho-2232604

ABSTRACT

Porcine epidemic diarrhoea virus (PEDV) is an emerging and re-emerging swine enterovirus that causes highly contagious diarrhoea and mortality in piglets. To better understand the current prevalence of PEDV in mid-west China, and to find out the reason for the re-emergence of PEDV from the viral genomic characteristics. Herein, we firstly investigated epidemiology of PEDV in mid-west China from 2019 to 2020. A total of 62.23% (257/413) of diarrhoea samples were positive for PEDV, and the PEDV-positive cases were mainly detected in winter. Then, we selected the SXSL strain as a representative strain to study the genetic and pathogenic characterization of PEDV pandemic strains in mid-west China. The recombination analysis showed that SXSL strain was a recombinant strain, and the major and minor parent strains of the recombination are CH/SCZJ/2018 strain and GDS48 strain, respectively. Complete genome sequencing and homology analysis showed that the S protein of SXSL strain contained multiple amino acid indels and mutations compared to the PEDV representative strains. Furthermore, we evaluated the effect of S protein on the infectivity and pathogenicity of PEDV by the PEDV reverse genetics system, and results showed that SXSL S protein increased the infectivity and pathogenicity of chimeric virus. Overall, our findings provided important information for understanding the roles of S protein in the prevalence of PEDV in mid-west China and developing vaccines based on PEDV pandemic strains.

2.
J Vet Res ; 66(3): 317-324, 2022 Sep.
Article in English | MEDLINE | ID: covidwho-2071039

ABSTRACT

Introduction: Porcine epidemic diarrhoea virus (PEDV) is an enteric pathogen causing porcine epidemic diarrhoea and acute gastroenteritis in pigs of all ages. Previous analysis of the viral genome of PEDV in Poland was only based on the spike protein (S) gene sequences and no analysis of other genes has been performed. The aim of this study was to analyse the envelope (E), membrane (M) and nucleocapsid (N) protein and open reading frame 3 (ORF3) gene sequences. Material and Methods: Viral RNA from 18 Polish pig faecal samples that were quantitative reverse transcription PCR-positive for PEDV was analysed in four genomic regions (E, M, N and ORF3). Results: Phylogenetic analysis based on these regions' sequences revealed that Polish PEDV isolates were highly related and were clustered into group G2a across the four genes compared. Moreover, the Polish strains were located in distinct subclusters on the phylogenetic trees, which suggests the presence of at least three independently evolving PEDV genetic lines circulating in Poland. The occurrence of unique mutations in the sequences of Polish PEDV strains suggests that PEDV continues to undergo evolutionary processes, accumulating the mutations necessary for viral fitness in its natural hosts. The Polish PEDV strains differed genetically from the CV777 vaccine strain, suggesting the risk of relatively low vaccine efficacy if this strain is used. Conclusion: Our results promote a better understanding of the genetic diversity of PEDV field isolates in Poland and highlight the importance of molecular characterisation of PEDV field strains for the development of an effective vaccine against PEDV.

3.
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(1):10-19, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-2056573

ABSTRACT

The aim of this study is to establish an indirect ELISA technique for detecting the SIgA antibody against porcine epidemic diarrhea virus (PEDV) to evaluate its mucosal immunity. Firstly, the S1D gene (534-789 aa) of PEDV was cloned into the pET-28a(+) vector, and induced in Escherichia coli BL21 (DE3) by IPTG, the product of which was in the form of inclusion bodies. According to Western-blot, the target protein S1D with antigenic activity was 32 ku in molecular weight and could be well detected. Then, the S1D protein was denatured by 8 mol/L urea, purified and gradient as the coating antigen to establish an indirect ELISA for detecting the PEDV specific SIgA antibody in nasal or oral mucus by optimizing conditions. And the optimal antigen coating concentration of ELISA was 2 micro g mL, the working concentrations of nasal mucus was 1:1 and the optimal blocking solution was 50 g/L skimmed milk, while the working concentrations and optimal blocking solution were 1:2 and 30 g/L BSA in oral mucus, the working concentrations of the enzyme-labeled antibody was 1:2 000 in nasal and oral mucus. Finally, 84 samples of oral and nasal mucus from immunized pigs were detected by S1D of ELISA, and the coincidence rate could reach 95.2% compared with purified PEDV of ELISA. In conclusion, the indirect ELISA established in this study provided a quick, simple, sensitive, and specific method to detect PEDV specific SigA for evaluating the level of PEDV mucosal immunity.

4.
Virus Res ; 322: 198954, 2022 Dec.
Article in English | MEDLINE | ID: covidwho-2050063

ABSTRACT

Porcine deltacoronavirus (PDCoV) and porcine epidemic diarrhoea virus (PEDV) are the main porcine enteric coronaviruses that cause severe diarrhoea in piglets, posing huge threat to the swine industry. Our previous study verified that the co-infection of PDCoV and PEDV is common in natural swine infections and obviously enhances the disease severity in piglets. However, the effects of co-infection of PDCoV and PEDV on intestinal microbial community are unknown. In current study, the microbial composition and diversity in the colon of piglets were analyzed. Our results showed that both of PDCoV and PEDV were mainly distributed in the small intestines and caused severe damage of ileum but not colon in the co-inoculated piglets. Furthermore, we observed that PDCoV and PEDV co-infection alters the gut microbiota composition at the phylum, family and genus levels. The abundance of Mitsuokella and Collinsella at genus level were significantly increased in PDCoV-PEDV co-infection piglets. Spearman's correlation analysis further suggested that there existed strong positive correlation between Mitsuokella and TNF-α, IL-6 and IL-8 secretion, these two factors may together aggravating the small intestine pathological lesions. These results proved there existed obvious correlation between the disease severity caused by PDCoV-PEDV co-infection and intestinal microbial community.


Subject(s)
Coinfection , Coronavirus Infections , Gastrointestinal Microbiome , Porcine epidemic diarrhea virus , Swine Diseases , Animals , Swine , Coinfection/veterinary
5.
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(12):1500-1508, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-2040500

ABSTRACT

Based on the M gene sequence of TGEV and PEDV and VP2 gene sequence of PoRV, the optimal reaction system and amplification procedure were established by optimizing primer, probe concentration and annealing temperature, and the Quantitative PCR method of TaqMan probes for three viruses is successfully established. On this basis, after further optimization of conditions, a triple real-time fluorescent quantitative PCR method for detecting TGEV, PEDV, and PoRV was established. The detection sensitivity of this method for TGEV, PEDV, and PoRV were 2.49 copies/ L, 4.36 copies/ L, and 4.96 copies/ L respectively. The maximum value of CV in repeated trials detected by TGEV, PEDV and PoRV were 2.5%, 3.8%, 4.3%, and the maximum value of CV in repeated trials between groups were 3.7%, 3.4%, 3.2%, which are no more than 5%.indicating that the established method has good reproducibility. Using this method to detect PRV, PCV1, and PRRSV virus samples, there is no cross-reaction, indicating that the method is specific. Using the established method to detect 40 clinical diseases, the samples were tested, and the positive rates of TGEV, PEDV, and PoRV were 5%, 30%, and 12.5%respectively. The mixed infection rate of TGEV and PEDV was 2.5%, the mixed infection rate of PEDV and PoRV was 5%. The results of the multiple fluorescence quantitative PCR method are consistent with those of the detection of a single fluorescent RT-PCR method, indicating that the established method has good clinical application value.

6.
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(11):1373-1378, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-2040499

ABSTRACT

In order to build a specific, sensitive and rapid detection method for PAstV3 detection, the PAstVB gene sequences in Genbank were used and the conserved region in ORFlb was selected to design specific primers and TaqMan probe. Clinical stool samples were collected and preliminary detected by this newly established real-time RT-PCR method after reaction systems and conditions optimization. This detection method established in this study has a good linear relationship with the standard curve, with R2 value up to 0.9971. The sensitivity is 100 times higher than conventional PCR method, The variation co-efficient of in-batch and inter-batch repeatability test is less than 2.0%, indicating good repeatability. The detection results of Clinical samples showed that the positive rate of this method is higher than conventional PCR method. The establishment of this method provides a rapid detection means for PAstV3 laboratory diagnosis and epidemiological investigation.

7.
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(11):1421-1427, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-2040498

ABSTRACT

Recently, the variation and isolation of porcine epidemic diarrhea Virus (PEDV) has been a focus of industry research. Whether porcine aminopeptidase (pAPN) is a functional receptor of PEDV infection is still controversial. Therefore, this article aims to review the latest progress on pAPN as a receptor of PEDV and its role during infection, to clarify whether pAPN is a functional receptor and to provide a reference for isolation and subsequent study of PEDV.

8.
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(11):1341-1347, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-2040497

ABSTRACT

The recombinant expression plasmid pIRES-S1 was constructed according to the gene sequence of PEDV S1 in NCBI (GenBank:JQ517274). The plasmid pIRES-S1 was transfected into ST cells by electrotransfer. After G418 pressurization screening, western-blot detection and suspension domestication, a stable transduction cell pool expressing S1 protein was obtained. The results of Western-blot showed that S1 protein have good reactivity. An indirect ELISA was established by using S1 protein as coating antigen, and the ELISA was used to detect PEDV clinical serum and PEDV negative serum of imported breeder pigs. Take the serum neutralization test as the standard, the results showed that the sensitivity of the ELISA was 96.3% and the specificity was 97.7%.It was significantly consistent with the serum neutralization test (kappa value=0.882, P < 0.05). The ELISA was used to detect the tracking serum of PEDV back-feeding pigs. The results showed that it could accurately evaluate the growth and decline of PEDV Ig G antibody level in infected pigs. Our results suggested that the ELISA based on S1 protein established in this study has high sensitivity and specificity. It could be used to detect PEDV antibody in clinical serum samples and provide an effective basis for immune evaluation of PEDV in pigs.

9.
Acta Microbiologica Sinica ; 8:3152-3165, 2022.
Article in Chinese | CAB Abstracts | ID: covidwho-2040441

ABSTRACT

Objective: To identify the key host protein that can regulate the replication of porcine epidemic diarrhea virus (PEDV).

10.
Journal of South China Agricultural University ; 41(5):27-35, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-2040361

ABSTRACT

Objective: To prepare monoclonal antibodies against porcine epidemic diarrhea virus (PEDV) N protein, and develop an indirect immuno-fluorescence assay method used for detecting PEDV. Method: The expressed recombinantly PEDV N protein was used as an immunogen and 8-week-old female BALB/c mice were immunized. Then their spleen cells with high antibody titer were isolated and fused with SP2/0 cells. The hybridoma cell lines secreting monoclonal antibodies against PEDV N protein were screened. In Vero cells infected with PEDV, monoclonal antibody of anti-PEDV N protein was used as the primary antibody and FITC-goat-anti-mouse IgG was used as the secondary antibody to develop indirect immuno-fluorescence assay method used for detecting PEDV. Result: The prepared hybridoma cell lines could stably secrete anti-PEDV N protein antibodies, ELISA antibody titer in cell supernatant was above 1:3 200, and in mouse ascites above 1:1 000 000. While monoclonal antibodies were applied in established indirect immuno-fluorescence assay, the optimal conditions were that cells were fixed with 80% () acetone at -20 degrees C for 30 min;The primary antibody was diluted 1 000 times by PBS buffer solution and incubated at 4 degrees C overnight;The secondary antibody was diluted 100 times by PBS buffer solution and incubated at 37 degrees C for 1 h. Transmissible gastroenteritis virus (TGEV), classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine reproductive virus (PRV), porcine enteric a corone virus (PEAV), porcine rotavirus (PoRV) and PEDV were detected by established indirect immuno-fluorescence assay method, only PEDV showed positive, all the else viruses showed negative.

11.
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(7):825-832, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-1994655

ABSTRACT

In order to establish a method for rapid differential identification of Senecavirus A (SVA) and en-cephalomyocarditis virus (EMCV), two pairs of corresponding specific primers were designed based on the highly conserved 3D genes of SVA and EMCV. And two different fluorescent labeled TaqMan probes were used to establish a dual TaqMan real-time PCR method for simultaneous detection of these two viruses, and we also optimize the reaction conditions. The results showed that the minimum detection of the method was 760 copies/ micro L and 98 copies/ micro L for SVA and EMCV. respectively, and it can specifically detect SVA and EMCV, and there was no cross reaction with CSFV, PRRSV and PEDV. The established standard curves showed good linear relationship. Repeated experimental group and inter-group coefficient of variation were less than 5%. The results indicated that the dual-quantitative PCR established in this study has the advantages of convenience, rapidity, good specificity. high sensitivity and good repeatability .and can be used for simultaneous detection of SVA and EMCV.

12.
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(5):537-544, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-1994651

ABSTRACT

Long noncoding RNA (lncRNA) is a type of non-coding RNA molecule longer than 200 nt, which plays vital roles in biological events. Our previous results demonstrated that the host's lncRNA expression profile was significantly changed after porcine epidemic diarrhea virus (PEDV) infection. In this study, one of the lncRNAs, lncRNA9606, was selected to investigate its impact on PEDV replication. First, the kinetics of lncRNA9606 expression in IPEC-J2 cells were examined at different time points after PEDV infection. The results confirmed that PEDV infection significantly upregulated the expression of lncRNA9606. The lncRNA9606 expression levels in different cells or tissues were evaluated and the results showed that the amount of lncRNA9606 in Peyer's patches and peripheral blood mononuclear cells were significantly higher than that in small intestinal epithelial cell lines. It was mainly localized in the nucleus. Further investigations indicated that over expression of lncRNA in LLC-PK1 cells significantly inhibited PEDV replication. In conclusion, lncRNA9606 can suppress the PEDV replication in LLC-PK1 cells.

13.
Acta Veterinaria et Zootechnica Sinica ; 53(5):1536-1543, 2022.
Article in Chinese | CAB Abstracts | ID: covidwho-1994512

ABSTRACT

This study aims to investigate the protective effect of infected piglets which were immunized with different dose of inactivated porcine epidemic diarrhea virus (PEDV) vaccines. The number of infective virus particles and total virus particles of PEDV with different concentrations were determined, and the mice were immunized with different concentration vaccine prepared as antigen, respectively. The humoral and cellular immune production were determined by ELISA antibody detection method, neutralization test and ELISPOT method. Vaccine with appropriate antigen content was selected to immunize piglets, then the antibody was determined. The relationship between concentrated vaccine and protective effect was studied by challenge experiment. The results showed that, when the antigen dose was equal or greater than 8x106 pfu.mL-1, the inactive vaccine could effectively stimulate mice to produce humoral and cellular immunity. The piglets immunized with 2 mL inactivated PEDV vaccine containing 8x106 pfu.mL-1 antigen could resist diarrhea and continuous viral shedding caused by PEDV challenge. Compared with the total number of virus particles, the number of infectious virus particles was significantly correlated with antibody production (r=0.998 1), and neutralization titer was significantly correlated with piglet protection (r=0.974 7). PEDV inactivated vaccine can provide good immune protection, in which the number of infectious virus particles is the key factor to improve the antibody level. Antibody titer, as an index of humoral immunity, is an important reference for judging immune protection.

14.
Chinese Veterinary Science / Zhongguo Shouyi Kexue ; 50(6):671-678, 2020.
Article in Chinese | CAB Abstracts | ID: covidwho-1975502

ABSTRACT

In present study, in order to develop a new and effective porcine epidemic diarrhea virus (PEDV)vaccine, three B cell epitopes and the truncated S1 gene of PEDV spike protein were combined and inserted into the immunodominant region of the HBcAg. Then the constructed recombinant plasmid HBcAg-PE was transformed to E. coli BL21 (DE3) for expression. After purification and identification by Western-blot, the expressed recombinant proteins HBPE were injected into BALB/c mice as vaccine antigen with different doses through intramuscular injection and its immune effect were preliminary evaluated. The results showed that the recombinant proteins HBPE was expressed as precipitation form and it could reacted specifically with PEDV-positive serum after purification and renaturation. Besides, the RH could induce anti-PEDV specific antibodies and the related Thl and Th2 cytokines in mice. The above results indicate that the recombinant compound epitope antigen of PEDV was successfully constructed. and its immunogenicity as a new vaccine candidate was evaluated in the mice in this study. The results of this study provided a new idea for the development of PEDV genetic engineering vaccine in the future.

15.
Acta Veterinaria et Zootechnica Sinica ; 53(4):1173-1181, 2022.
Article in Chinese | CAB Abstracts | ID: covidwho-1975364

ABSTRACT

The purpose of this study is to establish a blocking ELISA antibodies detection method for porcine epidemic diarrhea virus (PEDV). The purified N protein was used as the coating antigen, and the ELISA reaction conditions were optimized by the chess rboard titration. A blocking ELISA method for detecting PEDV antibodies was established, and its specificity, sensitivity and repeatability tests were carried out. One hundred and forty clinical serum samples were tested, and the results were compared with commercially IDvet PEDV indirect ELISA antibodies detection kit. The results showed that the best antigen coating concentration was 625 ng.mL-1, and the best dilution ratio of serum was 1:1;The best dilution of the HRP-conjugated antibody working solution was 1:5 000;There was no cross-reaction with healthy pig serum and the positive sera of common pig disease pathogens, such as classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), and transmissible gastroenteritis virus (TGEV). The sensitivity of PEDV positive serum was 1:16, which was equivalent to that of IDvet ELISA kit (titer 1:32). The coefficient of variation of within-run and between-run repeatability test is less than 10%, so it showed that the blocking ELISA established in this study had good repeatability and stability;the kappa value of detected 140 clinical porcine serum using this method was 0.87 when compared with IDvet ELISA. The above results indicated that the established blocking ELISA method for detecting PEDV antibodies in this study could be applied to the prevention and control of PEDV, epidemiological investigation and the monitoring of antibody levels after vaccine immunization.

16.
Transbound Emerg Dis ; 2022 Aug 02.
Article in English | MEDLINE | ID: covidwho-1973746

ABSTRACT

The aim of this study was to characterize the infection of weaned pigs with swine enteric coronavirus (SeCoV) - a chimeric virus most likely originated from a recombination event between porcine epidemic diarrhoea virus (PEDV) and transmissible gastroenteritis virus, or its mutant porcine respiratory coronavirus - and two PEDV G1b variants, including a recently described recombinant PEDV-SeCoV (rPEDV-SeCoV), as well as to determine the degree of cross-protection achieved against the rPEDV-SeCoV. For this purpose, forty-eight 4-week-old weaned pigs were randomly allocated into four groups of 12 animals. Piglets within each group were primary inoculated with one of the investigated viral strains (B: PEDV; C: SeCoV and D: rPEDV-SeCoV) or mock-inoculated (A), and exposed to rPEDV-SeCOV at day 20 post-infection; thus, group A was primary challenged (-/rPEDV-SeCoV), groups B and C were subjected to a heterologous re-challenge (PEDV/rPEDV-SeCoV and SeCoV/rPEDV-SeCoV, respectively), and group D to a homologous re-challenge (rPEDV-SeCoV/rPEDV-SeCoV), Clinical signs, viral shedding, microscopic lesions and specific humoral and cellular immune responses (IgG, IgA, neutralizing antibodies and IgA and IFN-γ-secreting cells) were monitored. After primo-infection, all three viral strains induced an undistinguishable mild-to-moderate clinical disease with diarrhoea as the main sign and villus shortening lesions in the small intestine. In homologous re-challenged pigs, no clinical signs or lesions were observed, and viral shedding was only detected in a single animal. This fact may be explained by the significant high level of rPEDV-SeCoV-specific neutralizing antibodies found in these pigs before the challenge. In contrast, prior exposure to a different PEDV G1b variant or SeCoV only provided partial cross-protection, allowing rPEDV-SeCoV replication and shedding in faeces.

17.
Acta Agriculturae Jiangxi ; 34(2):160-165, 2022.
Article in Chinese | CAB Abstracts | ID: covidwho-1964892

ABSTRACT

In this study, 650 tissue samples which were collected from 16 pig farms in Hubei Province, were used to detect porcine circovirus (PCV) and Porcine epidemic diarrhea virus (PEDV). The results showed that the positive rates of PCV1, PCV2, PCV3 and PEDV single infection were 1.08%, 4.15%, 2.46% and 6.46%, respectively. In the double infections, PEDV+PCV2 had the highest positive rate of 3.54%, followed by PCV2+PCV3, with a positive rate of 1.54%. In multiple infections, PEDV+PCV2+PCV3 had the highest positive rate of 2.00%. The results indicated that the positive rates of PEDV and PCV were decreased compared with the previous studies, but the prevalence of PEDV and PCV was still wide in Hubei Province, and most of which were co-infection.

18.
Journal of Yangzhou University, Agricultural and Life Sciences Edition ; 42(6):48-53, 2021.
Article in English, Chinese | CAB Abstracts | ID: covidwho-1964809

ABSTRACT

As a member of the family Picornaviridae, porcine sapelovirus (PSV) is often infected with porcine epidemic diarrhea virus, teschovirus and so on. In recent years, PSV has been isolated from porcine in many provinces of China. It suggests that it is necessary to strengthen the research on PSV. In this study, according to the sequence of PSV HuN2 strain, VP1 gene was inserted into the pGEX-6 P-1 vector, and expressed the recombinant protein. BALB/c mice aged 6-8 weeks were immunized according to the standard procedure. After the third immunization, the mouse orbital blood was collected to identify the antibody level. The highly positive mouse spleen cells were selected for cell fusion. The positive hybridoma cells and two subclones were screened by IFA method, and then a PSV VP1 monoclonal antibody was obtained, named as 33-2 A. The results of IFA showed that PSV could be recognized by 33-2 A MAb, and specific green fluorescence appeared in the cytoplasm;The results of WB and IP showed that PSV infected porcine cell could specifically bind to 33-2 A, and there was a specific band at 32 ku. We also identified the B-cell antigen epitope of 33-2 A, it was at amino acids 40-46 of PSV VP1 protein, and the polypeptide sequence was 40PALTAAE46. The results showed that the monoclonal antibody can react with PSV VP1 protein. The epitope was analyzed with the PSV sequences uploaded in NCBI, 33-2 A antibody can react with most PSV strains and has a certain universal to PSV. This study laid a foundation for the study of the etiology and pathogenesis of PSV.

19.
Animal Husbandry and Feed Science ; 43(4):14-18, 2022.
Article in Chinese | CAB Abstracts | ID: covidwho-1964618

ABSTRACT

[Objective] To identify the B-cell epitope peptide of porcine epidemic diarrhea virus (PEDV) S2 gene by combinative use of bioinformatics software and monoclonal antibody technology. [Method] The B-cell epitope of PEDV S2 gene was screened using CLC Sequence viewer 6.8 software and IEDB online database, and the obtained epitope peptide was synthesized artificially. Female BALB/c mice were immunized with the conjugate of epitope peptide and keyhole hemocyanin (KLH) as antigen. Mice with higher antibody titers were identified by ELISA assay and then received an additional immunization. The spleen of the mice was taken 3 days post immunization to prepare the splenocyte suspension for cell fusion. The cells were grown on HAT selective medium to screen for effective hybridoma cells. The positive clones screened by ELISA assay were then used for expanding culture. Positive hybridoma cells were intraperitoneally injected to mice and ascites were collected. ELISA assay was used to determine the antibody titers in mice ascites and in the supernatants of monoclonal cell strains. The cells with the highest antibody titers was used as cell strain for subsequent use. [Result] The selected B-cell epitope peptide sequence was MQYVYTPTYYML Following immunization with the peptide antigen, the serum antibody titer before cell fusion reached 1:2 000. The ELISA assay of ascites from BALB/c mice and the supernatants from monoclonal cell strain cultures demostrated that the antibody liter reached 1:4 000. [Conclusion] The B-ell epitope of PEDV S2 gene was identified, which may be helpful for the vector construction of a epitope based peptide vaccine against PEDV.

20.
Jiangsu Journal of Agricultural Sciences ; 38(2):422-428, 2022.
Article in Chinese | CAB Abstracts | ID: covidwho-1893038

ABSTRACT

In this study, we detected the viral load and protein expression of porcine epidemic diarrhea virus (PEDV) after overexpression and inhibition of integrin avbeta1 on the surface of Vero cells, and then cleared the role of integrin avbeta1 in the PEDV infection process. The results showed that the viral load and protein expression were significantly increased in the Vero cells which overexpressed integrin avbeta1, and the viral load and protein expression were significantly reduced in the Vero cells with silent integrin avbeta1 gene. Integrin avbeta1 promotes PEDV to infect Vero cells.

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