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Hollow viscous perforation is one of the most common emergency dealt by general surgeons worldwide. A high degree of suspicion is required and prompt management is warranted to reduce morbidity and mortality. Here we present a study of perforative peritonitis in our medical college at the outskirt of Udaipur city in the state of Rajasthan, India during the Covid-19 pandemic. During the one year study period from September 2020 to August 2021, a total of 16 cases of hollow viscous perforation admitted and treated. The number is relatively low because of the pandemic and there was a period of total lockdown with negligible patient footfall. All patient had free gas under diaphragm in plain x-ray and all of them were scheduled for emergency laparotomy after adequate resuscitation. Emphasis given on the location of perforation, etiology, organism on culture of peritoneal fluid and procedure undertaken. Copyright © 2022, Dr Yashwant Research Labs Pvt Ltd. All rights reserved.
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Background: Rabies is a disease transmitted mostly through animal bites in humans, and seasonal variation in animal bites has been reported by different studies. There has been no study in India using time series analysis for studying monthly variation in animal bite cases. Aim: (a) To find out long-term trends and monthly variations in new animal bite cases. (b) To make projections for new animal bite cases. (c) To find out the difference between actual and projected new animal bite cases following the COVID-19 pandemic. Methodology: A retrolective, record-based study was conducted in a tertiary care facility, Jaipur, and data of new Category II and Category III animal bite cases were taken from January 2007 to December 2021. A multiplicative model was used for conducting time series analysis. The projected monthly number of cases was estimated using the line of best fit based on the least square method. Result: An increasing trend in the annual number of animal bite cases was observed from 7,982 in 2007 to 10,134 in 2019. The monthly index was lowest for the months July to November (0.88 to 0.95), peaked in January (1.14), remained higher from January to June, and fell in July (0.95). The monthly number of new animal bite cases from April 2020 to December 2021 was significantly lower than the projected number (P-value < 0.001).
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Background: Corona virus disease is an ongoing pandemic. COVID-19 had put cumbersome mental and physical pressure on the healthcare staff which may lead to burnout in them. Also there is paucity of Indian literature regarding prevalence of burnout in rural health care staff. Objective(s): To study the prevalence of burnout and its association with the determinants among the healthcare workers in rural population of a Ajmer district, Rajasthan. Methodology: A Cross Sectional Questionnaire based study was conducted from January 2021 to June 2021 on 173 healthcare staff of rural population in a Ajmer district, Rajasthan after ethical clearance. Copenhagen Burnout Inventory was used to assess the burnout among Health Care Workers and a semi structured Performa was used to evaluate the demographic and clinical determinants of burnout. Prevalence of burnout was determined and the association of determinants with burnout was assessed. Result(s): The prevalence of personal burnout, work related burnout and pandemic related burnout in health care workers was 42.5%, 33.75% and 49.37%. Pandemic related burnout was significantly greater than personal burnout and work related burnout in health care workers. Doctors, redeployed healthcare staff and staff having covid positive case in family have significantly greater burnout. Supportive work environment and adequate protective measures at workplace significantly reduces the burnout among health care staff. Conclusion(s): Almost half of the rural health care workers are burnout. Protective authoritative and individual measures are needed to prevent burnout in HCWs. Copyright © 2022 Authors. All rights reserved.
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This research demonstrates that the geospatial method adds greatly to identifying pre-and post-COVID 19 pandemic situations and aiding in sound decision-making, not only in Rajasthan but globally. Results reveal that hotels with a more management mindset are more likely to use dynamic pricing techniques. When the pandemic's intensity is severe, hotel managers resort to a more streamlined booking portfolio. Hotel owners and the pricing community as a whole may use our theoretical implications and practical management levers to increase profits as much as possible during the epidemic. This research set out to learn how the hotel industry's top brass think the sector would fare in the near future, and whether or not the response to the shutdown and following decline in domestic and international travel should have been handled better. Research like this is important because it draws attention to the fact that more precautions may have been taken to mitigate the disaster's effects. Qualitative surveys sent through email to partners in the hotel sector and their responses provide light on the days and hours leading up to the lockdown. Copyright © 2022, Anka Publishers. All rights reserved.
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Introduction: Bharat Biotech International Ltd in partnership with National Institute of Virology (NIV), has developed an indigenous whole virion inactivated Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) viral vaccine BBV-152 (Covaxin), formulated with Toll Like Receptors 7/8 agonist Imidazoquinoline (IMDG) molecule adsorbed to alum (Algel). Variety of factors other than environmental ones can affect vaccines efficiency outside the strict setting of clinical trials, like how the vaccine is stored or transported, and even how patients are vaccinated. In addition, the intrinsic capacity of the recipient to respond to a vaccine which is determined by sex, genetic factors, age, psychological stress, nutrition and other diseases are also likely to have an impact. Aim(s): To determine the safety, reactogenicity and immunogenicity of the inactivated whole virus vaccine (Covaxin) amongst hospital-based population groups. Material(s) and Method(s): The prospective analytical study was conducted in the Department of Microbiology, Sawai Man Singh Medical College, Jaipur, Rajasthan, India, from January 2021 to March 2021.The study primarily included Healthcare Workers (HCWs) employed at SMS Medical college and attached hospitals. In-vitro quantitative IgG antibodies against SARS-CoV-2 spike Receptor Binding Domain (RBD) were measured using Chemiluminescence Immunoassay (CLIA) based Advia centaur SARS-CoV-2 IgG, manufactured by Siemens Pvt Ltd, Munich, Germany, as per manufacture's instructions. Result(s): Out of total 223 individuals, 61.88 % (138/223) showed neutralising antibody titre of >1 index value by CLIA, rest 38.12% (85/223) were non reactive i.e., titre <1 index value, after four weeks of receiving first dose of Covaxin. After 2 to 4 weeks of receiving second dose 84.30% (188/223) showed neutralising antibody titre of >1 index value by CLIA, rest 15.70% (35/223) were non reactive i.e., titre <1 index value. After receiving first dose, 100% (223/223) of the participants developed localised pain and bodyache 33.63% (75/223). None of the participants showed any anaphylactic reaction or any emergency condition just after vaccination. Conclusion(s): Covaxin is a well-tolerated vaccine, and induces good humoral response against SARS-CoV-2 with a significant rise in the neutralising antibody titres. Copyright © 2022 Journal of Clinical and Diagnostic Research. All rights reserved.
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Background: The SARS-CoV-2 virus replicates rapidly, triggering a storm characterized by increased levels of cytokines such as IL-6. Such an inflammatory response causes inflammation of the respiratory system and other bodily systems, with subsequent occurrence of ARDS or respiratory failure. The estimation of IL-6 levels could be an important tool to assess disease severity in COVID-19 patients. Methodology: A retrospective cohort study was conducted on 45 patients in Department of Medicine, MDM Hospital, Dr. S.N Medical College, Jodhpur, Rajasthan. A predesigned and pre-structure Performa was used. Conclusion: IL-6 is an adequate predictor of severe disease in patients infected with the COVID-19 virus. The finding of current study guide clinicians and healthcare providers in identifying potentially severe or critical patients with COVID-19.
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Introduction: While managing COVID-19 patients, the healthcare professionals are at higher risk for contracting the infection and also could be a potential source of transmitting the disease in the community unknowingly. Therefore awareness [knowledge, attitude, and practice (KAP)] among healthcare professionals becomes of utmost importance. Methods: We conducted this observational cross sectional study to evaluate the knowledge, attitude and perceived practices toward COVID-19 among HCWs using a self-administered questionnaire at tertiary level healthcare facility in western Rajasthan. The questionnaire was shared with all the healthcare professionals of the pre identified tertiary care facilities through electronic mail (e-mail) and the responses received were recorded and analyzed. Results: A total of 59 responses were recorded. Out of the total participants, 61% were aware of national COVID-19 helpline numbers, 54.2% answered correctly about Hydroxychloroquine prophylaxis, 13.6% answered appropriately on COVID-19 testing. Almost 96.6% acknowledged to wear a medical mask however only 55.9% participants answered correctly regarding using a face mask. 52.5% responded that ash collection as a ritual may be allowed after funeral (cremation) of the body of a COVID-19 patient. More than 90% of participants acknowledged to clean their hands > 6-10 times in a day. 72.9% of participants admitted to open the MoHFW website to keep themselves updated on COVID-19 in India and 66.1% have 'Aarogya Setu' application in their mobile phone. However only 23.7% have ever used central helpline number or email-ID to get information on COVID-19. Conlcusion: We concluded that healthcare professionals were aware of the management strategies and treatment protocol however there is significant differences in the KAP of HCW sub groups. It also becomes important to study the KAP in various other populations (general populations, close contacts of COVID-19 etc.) for planning effective intervention strategies for them.
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Background: Groundnut (Arachis hypogaea L.) is an important legume crop of tropical and sub-tropical areas of the world. This crop suffers from several diseases like early leaf spot, late leaf spot, rust, groundnut bud necrosis, peanut clump and collar rot etc. But collar rot caused by Aspergillus niger van Teighem is one of the most important seed and soil borne diseases causing huge economic (40-50 per cent) loss in India and abroad. Methods: A roving survey was conducted to assess the incidence of collar rot in eight major groundnut growing districts of western Rajasthan i.e. Bikaner, Jodhpur, Churu, Jalore, Jaipur, Sikar, Nagaur and Dausa during Kharif 2018. A field experiment was carried out for two consecutive years (2019 and 2020) to manage the disease through altering micro-climate under canopy with six levels of seed rates (80, 85, 90, 95, 100 and 105 kg/ha). Result: Our investigations cleared that this disease is prevalent in all the 200 fields surveyed in eight districts of Rajasthan with 22.99 per cent overall disease incidence which varied from 17.84 to 32.38 per cent with monetary losses. Maximum disease incidence was recorded in Jaipur (28.85%) district while it was lowest in Nagaur district (20.15%). Higher disease reduction (12.97%) with increased pod yield (27.65%) was observed with higher seed rate of 105 kg/ha as compared to standard recommended seed rate (80 kg/ha) as this pathogen is favoured by higher temperature and low soil moisture, dense canopy provides shade that increases humidity under the cropped area and lowers temperature. As a result of this, plant mortality can be compensated by increasing plant population through seed rate, as it modifies the microclimate and generate an environment which is less favourable for developing collar rot disease of groundnut. [ FROM AUTHOR] Copyright of Legume Research: An International Journal is the property of Agricultural Research Communication Centre and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)
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The lockdown restrictions during the COVID-19 pandemic provided a 'path' of reinstatement of the air quality globally. Despite several financial challenges, air quality improvement positively impacted the environment due to lockdown in the worst pandemic situations. The present study assessed the air pollution scenario in the post lockdown phase in the seven major metropolises of Rajasthan, namely, Jodhpur, Alwar, Jaipur, Kota, Pali, Ajmer, and Udaipur) in the recent pandemic year 2020. The air pollution scenario is determined with the help of the Air Quality Index (AQI) and the concentration level of PM2.5 PM10, NO2, and SO2. This study reveals that Most Cities of Rajasthan are violating India's national ambient air quality standards (NAAQS). It is found that Jodhpur is on rank first in terms of pollution levels, followed by Alwar, Jaipur, Pali, and Udaipur. The pollution level was higher before the lockdown period then reduced to a certain level due to restricted activities in lockdown. The pollution level is not rapidly increased after lockdown due to rainfall occurred in Rainy season from Southwest monsoon. Winter Season consists of higher concentration levels of pollutant and higher than Before Lockdown Period. The study shows the Significant impact of lockdown in reducing air pollution levels in cities. But imposing lockdown in a city or country is not a permanent solution to curb air pollution. So, regulating agencies and stakeholders should implement better control and reduction technologies for Indian cities. © The Electrochemical Society
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Objective: To explore various post covid immune mediatedneurological manifestations in children Background: The neurological manifestation following a SARS-CoV2 infection is varied and till now there are only few studies reported regarding the same. Our study aimed to identify the varied spectrum of neurological manifestation following SARS-CoV2 infection. Design/Methods: Retrospective data were collected from May 2021 to September 2021, including all children aged from 1month to 18 years of age who presented to our pediatric emergency or OPD (a tertiary care center from western Rajasthan, India) with the neurological manifestation with history of COVID-19 infection or exposure and positive SARS-CoV-2 serology. Those who are RT PCR positive were excluded. The neurological manifestations were further categorized in a pre-designed proforma. Results: Case records of the 18 children who fulfilled the criteria were included in the study, among them 7 (38.8%) were male and 11 (61.1%) were female. Predominant presentation in our study group was seizures (6/18) and Gullian Barre Syndrome (5/18). Other manifestations included stroke (2/18), ADEM (1/18), MS (1/18), LETM (1/18), Autoimmune encephalitis[NMDAR](2/18). In our study group, 13/18 (72.2%) required immunomodulatory therapy either IVIG or high dose methylprednisolone pulse therapy. Steroids were used upfront in patients with elevated inflammatory markers. Cerebrovascular complications in children were less common compared to adults. Most of the children had favourable outcomes except for one mortality in our cohort. Conclusions: Delayed complications following SARS-CoV2 infection with varied manifestations are seen in children. A temporal correlation between the COVID 19 infection and the increasing number of neurological cases after the second wave was noted. Steroids are beneficial while treating such patients. Testing for SARS-CoV2 serology during the pandemic can give a clue to the underlying etiology. However, further studies are required to understand the CNS effects of SARS-CoV2 infection in children.
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Introduction: Consequent upon continued nationwide lockdown to check the spread of COVID-19 pandemic, online teaching and learning has emerged as a new mode to continue the regular educational programs in India. It is vital to assess the perception of this new method by various stakeholders of educational sector. Objectives: The objectives included identification of the problems and benefits felt by medical students and college teachers about online classes and to assess its effectiveness on attendance and academic performance of students. Methods: The cross-sectional study was conducted during March-October 2021 among 150 first MBBS students admitted in 2020 in a medical college at Udaipur, Rajasthan. The participants who gave informed consent and attended three online and offline monthly tests were included and the rest were excluded. The quantitative techniques included frequency tables, mean, standard deviation and ‘t-test’. Results: Among 100 respondents, 48% were male and 52% were female. The major problems faced by students included lack of space at home to attend class (71%), interrupted internet connectivity (42%), missing interaction with stakeholders (>70%) and mismatch in theory and practicals (69%). The benefits included homemade food, family care, risk minimization of COVID-19 and regular parental monitoring. The major advantages felt by teachers included continued teaching (90.90%) and saving time (100%) in taking attendance in online mode. The average attendance for online classes was significantly higher over offline classes (p<0.0001), whereas the average retention of knowledge level as evidenced by average marks was significantly lower for online class tests compared to offline class tests (p<0.0001). Conclusion: The higher attendance does not reveal higher knowledge retention during online mode of classes.
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Background: Covid-19 has become a global threat. All most all the countries are facing this pandemic. This pandemic has greatly affected the medical education and medical students who are tomorrow's doctors. So, they need to have a wide awareness regarding the knowledge, attitude, and practice towards this Covid-19 infection. Objective: To study the knowledge, attitude, and practice of medical students regarding the Covid-19 pandemic. Material and Method: This descriptive and cross-sectional study was conducted on 260 medical students of J.L.N. Medical College, Ajmer (Rajasthan). A total of 20 questionnaires in which, 10 in the knowledge section, 5 each in the attitude and practice section, were provided in July - August, 2021. Results: A total of 260 responses were collected and studied. The male to female ratio in the study population was 0.91. A total of 54.46% of students gave correct responses regarding the knowledge section. 87.31% of students show a positive attitude towards vaccination. Overall positivity regarding attitude was 76.61%. 95 % of Students were proactive regarding washing their hands, and 96.53% were proactive about face masks which are the important measures for the prevention of Covid-19 infection. Conclusion: This study focused the knowledge, attitude and practice (K.A.P.) of medical students regarding Covid-19 pandemic. Overall K.A.P. score was 75.92%, indicating that medical students have good awareness regarding this pandemic. However some students are required to be more educated on knowledgeable sections such as various diagnostic methods for Covid-19 and practices on proper disposal of N-95 masks etc.
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Poultry enteritis is an important multifactorial disease. Avian coronavirus (ACV) is one of many viruses related to enteric diseases and infectious bronchitis. Aim of this study was to find out the occurrence of ACV in enteritis affected broiler, molecular detection, phylogenetic analysis of avian corona virus and to examine intestine and liver for gross and microscopic lesions. Dead poultry birds (N=604) affected with enteritis were examined for presence of ACV. Intestinal samples of four birds were pooled to make one biological sample enteric ACV as the causative agent of enteritis in commercial poultry sector in and around four major districts of Rajasthan by RT-PCR. Molecular characterization was carried out by partial gene sequencing. Liver and intestine were examined grossly during post-mortem and by histopathology. Out of 151 pooled samples tested 51 (35.10%) were found positive for ACV. Prevalence of enteric ACV was highest in Ajmer (45.94%) and lowest in Dungarpur (23.07%) districts. 0-1 weeks age chicken flocks were found more susceptible for enteric ACV with 33.80% prevalence. Comparison of ACV sequence of this study revealed nucleotide (nt) identities from 99.44% among themselves, 99.44% with ACV from abroad. The amino acid (aa) identities of ACV of this study among themselves and with abroad sequences was 47.06 to 100%. Further severe congestion in intestine and necrotic patches on liver were recorded. Histopathology showed severe villous atrophy, congestion and cystic glands in sub-mucosa in intestine and severe congestion and haemorrhages along with infiltration of inflammatory cells in liver parenchyma.
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Introduction: Although isolation and quarantine are important measures to curb the exponential growth of the prevailing Coronavirus disease 2019 (COVID-19) pandemic, but at same time this can impose psychological issues among the affected population and also to their family members. Aims: To evaluate the mental health problems, their severity and associated factors in quarantined population during the COVID-19 pandemic. Materials and Methods: This cross-sectional study was conducted among 207 quarantined subjects at different quarantine centres of Ajmer, associated with JLN Medical College, Ajmer, from August 2020 to October 2020,after getting approval from ethics committee of the centre. All the consenting quarantined subjects who were of age 18 years and above, irrespective of their gender were enrolled in the study. For the assessment of psychiatric morbidity, participants were screened using Mini-International Neuropsychiatric Interview (MINI) 6.0.0. Finally, the relevant psychiatric assessment tools like Hamilton Rating Scale for Depression (HAM-D),Hamilton Anxiety Rating Scale (HAM-A) and Yale-Brown Obsessive Compulsive Scale were applied to assess the severity of the disorders. Pearson correlation analysis was used to evaluate the relationship among various clinical variables.The level of significance was considered at p-value lt;0.05. Results: Majority 85 (41%) subjects belonged to the age group 31-40 years of age. Around 116 (77.3%) participants were male. Around 51 (24.6%) presented moderate depression and 25 (12%) presented with severe depression. Also 182 (87.9%) presented with moderate anxiety. The study showed a statistically significant association between depression/anxiety and substance abuse, insomnia, co-morbidities, suicidal ideation/attempts. Conclusion: The findings of the present study concluded that a significant proportion of the quarantined population suffered from psychological issues. So, the psychological impact of a mandatory quarantine should be weighed more thoughtful and in an evidence based manner.
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During COVID-19 pandemic, one of the most common arrythmia reported with this illness is sinus bradycardia. Treatment for COVID-19 and associated cardiac dysfunction is still evolving. Temporary pacemaker insertion is difficult due to pandemic and risk of spread of infection to the additional staff involved. Orciprenaline stimulates the sino-atrial and atrioventricular nodes and accelerates atrioventricular conduction. Theophylline improves sinus node function in subjects with sinus bradycardia and enhances atrioventricular nodal conduction We report a case series of 10 patients admitted in dedicated COVID-19 ICUs and developed sinus node dysfunction. All of these patients were started on etophylline and theophylline prolonged release tablet (150 mg) once a day. On subsequent follow up after 72 hours, all patients reported heart rate well within normal range. COVID-19 virus directly involves the myocardium by entering the cardiac myocytes resulting in inflammation and injury. As the sinus bradycardia due to COVID-19 is usually transient and respond well this drug, short course of this drug could be added to treat this arrythmia in future.
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Introduction: COVID-19 spread was due to the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Even today, COVID cases are being continually recorded. On this basis, it can be said that there is still the danger of COVID-19 cases getting increased at a rapid rate. There is no way of distinguishing the clinical findings and radiological findings of secondary fungal infection from that of COVID-19 pneumonitis and pneumonia. Aim: To Assess the MRI Evaluation of Cases of Mucormycosis after COVID-19”. Material and Methods: In the current study, the researcher conducted an observational study at Pacific Medical College, Udaipur, Rajasthan, India. Data for all the confirmed mucormycosis cases among patients with and without COVID-19 reported from September 2020 to December 2020, for the current study was collected. The researcher used SPSS Statistics 21.0 for performing an analysis of the data obtained from the health care centre. The descriptive statistics were measured and analysed using frequencies, mean, standard deviations, and median. Results: Out of the 286 cases, 65% (N = 185) had CAM (COVID-19 Associated Mucormycosis), with the mean age of 52 (SD = 16) years. Furthermore, 75% (N = 214) of the entire study population was male;and the remaining 25% (N = 72) were female. The prevalence of CAM was 0.28% and the range was 0.04% to 0.60%;on the other hand, CAM prevalence in ICU patients was determined to be 1.9% and the range was o.68% to 2%. Conclusion: From the current results, it can be concluded that Uncontrolled Diabetes Mellitus, found to be among 63% of the participants, was one of the common diseases in both CAM as well as non-CAM groups. In addition, the rhino-orbital area was among the most well-known sites of mucormycosis, with 58% participants, followed by rhino-orbital-cerebral, pneumonic, and other such areas.
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Introduction: Coronavirus Disease-19 (COVID-19) infection is associated with high rates of pulmonary and extrapulmonary complications that may continue to incur morbidity, disability and delayed mortality in survivors. These include hyperglycaemia, cardiac injury, acute ischaemic or haemorrhagic stroke, neurological deficits, acute kidney injury and liver injury. Aim: To describe symptoms and complications being faced by COVID-19 recovered patients, as well their socio-demographic profile and co-morbidities. Materials and Methods: This was a cross-sectional descriptive study conducted for the period of 12 months from April 2020-March 2021. Out of nearly 10,000 recovered COVID-19 patients, 1000 (calculated sample size) patients were selected randomly. The patients were categorised gender-wise (male and female) and locality-wise (urban and rural) and an attempt was made to find if any significant difference exists in the symptoms and complications based on above categorisation. The test used for this purpose was Chi-square test and Fisher’s-exact test. Results: Mean age of participants was 50.2±15.7 years and 43.8% had co-morbidities. Common complications included hyperglycaemia (n=28), acute kidney injury (n=8), acute liver injury (n=5), cardio-vascular accident and stroke (n=5), septicaemia (n=8), ischaemic heart disease (n=7), deep vein thrombosis (n=2), cytokine release syndrome (n=10) and post COVID-19 fibrosis (n=3). For septicaemia, a statistically significant difference (p<0.001) was found between urban and rural areas whereas no significant difference in post COVID-19 complications between males and females was observed. conclusion: The most common co-morbidity was diabetes mellitus and most common complication reported was hyperglycaemia.
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Adolescence is a period during which individuals' transit from puberty to adulthood. Children go through many changes throughout this time, including biological, cognitive, and emotional changes. Excessive stress caused by studies, high expectations, and lack of capacity to maintain studies is referred to as academic stress. The study's major goal was to assess the academic stress among rural adolescents owing to COVID- 9. The study was conducted in Udaipur district of Rajasthan. For the sample selection from four villages having Sr. Sec, schools were randomly selected. From selected schools, 180 students of age group of 16-18 years were selected randomly. The sample consisted equal no. of adolescent boys and adolescent girls. Slightly modified Academic stress scale developed by Rao (2012) was used to assess academic stress in adolescent boys and girls. Collected data was further classified, in tabulated form and analyzed through using suitable statistical measures. Results indicated that adolescents' boys and girls faced moderate to high academic stress. Furthermore, girls were facing high academic stress as compared to boys.
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Background: Corona virus disease 2019 is a highly infectious disease which is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2. SARS-CoV-2 is transmitted from person to person mainly by respiratory droplets and aerosols as well as by direct or indirect contact. Aims and objective: To compare different RNA extraction methods for detection of SARSCov-2 RNA from nasopharyngeal and oropharyngeal swabs using three different methods which are based on different techniques. Material and methods: This analytical observational study was conducted in the department of Microbiology, Sawai Man Singh Medical College Jaipur, Rajasthan from December 2020 to January 2021. We selected 200 confirmed positive (extracted by Easy Mag automated system) (remnant) samples showing a wide range of different Ct values and 20 confirmed negative samples stored in Viral Transport Media VTM for this study. In order to compare quality of three extractions methods, all samples were aliquoted separately for each extraction technique. (1) Extraction by manual method (spin column base): was done by as per manufacturer’s instructions. (2) Extraction by QIA cube HT (vaccum column base): was done by as per manufacturer’s instructions. (3) Extraction by Perkins Elmer chemagic 360: (magnetic beads based). Result: A panel consisting of 200 Covid-19 positive and 20 Covid-19 negative samples were extracted by three methods (i.e. Manual column based, automated column-based and automated magnetic beads-based method). The extracted material/elutes were put for realtime RT-PCR assay for the detection of SARS CoV-2 RNA. There was no major difference seen in individual samples’ ct values between three extraction system. CONCLUSION: In conclusion, we recommended all three RNA extraction methods (i.e. magnetic beads & silica column-based) are interchangeable in a diagnostic workflow for the SARS CoV-2 by RTPCR and can be taken into account for SARS CoV-2 detection in possible future shortage of one kit or times of crisis in such pandemic time.