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1.
World Journal of Traditional Chinese Medicine ; 8(4):463-490, 2022.
Article in English | EMBASE | ID: covidwho-2066828

ABSTRACT

Curcumae Longae Rhizoma (CLR) is the rhizome of Curcuma longa L. Pharmacological studies show that CLR can be used to treat cervical cancer, lung cancer, lupus nephritis, and other conditions. In this paper, we review botany, traditional application, phytochemistry, pharmacological activity, and pharmacokinetics of CLR. The literature from 1981 to date was entirely collected from online databases, such as Web of Science, Google Scholar, China Academic Journals full-text database (CNKI), Wiley, Springer, PubMed, and ScienceDirect. The data were also obtained from ancient books, theses and dissertations, and Flora Reipublicae Popularis Sinicae. There are a total of 275 compounds that have been isolated from CLR, including phenolic compounds, volatile oils, and others. The therapeutic effect of turmeric has been expanded from breaking blood and activating qi in the traditional sense to antitumor, anti-inflammatory, antioxidation, neuroprotection, antibacterial, hypolipidemic effects, and other benefits. However, the active ingredients and mechanisms of action related to relieving disease remain ill defined, which requires more in-depth research and verification at a clinical level.

2.
FEBS Open Bio ; 12:295, 2022.
Article in English | EMBASE | ID: covidwho-1976653

ABSTRACT

This study was aimed to detect trace amounts of drugs in littoral crustaceans of ancient Lake Baikal. For the first step, these drugs were presented by ibuprofen and azithromycin. Ibuprofen is widely used to reduce fever and pain. Azithromycin is an antimalarial drug, that was recently reported to be active against acute respiratory coronavirus syndrome. Endemic crustaceans related to species Eulimnogammarus verrucosus were collected near village B. Goloustnoe. Animals were homogenized with acetonitrile and shaken intensively. Then precipitation of proteins was performed with a solution of TCA, and centrifuged. The supernatant was removed and filtered in chromatographic vials. The analysis was performed using an HPLC Agilent 1290 Infinity coupled with Agilent 6470 Triple Quadrupole. Mobile phase A consisted of 0.1% formic acid in the water, and mobile phase B was presented by 100% acetonitrile. The registration of ions was performed in different modes, such as: Full scan, MRM, Product Ion. For reliable identification of drug contaminants, we used the pharmaceutical substances of azithromycin and ibuprofen. Thus, here we first time reliably detected drugs in the amphipods of Lake Baikal and demonstrated the contamination of the Baikal ecosystem by azithromycin and ibuprofen. It indicates the ability to accumulate the drug substances from wastewater by amphipods, and increasing anthropogenic load on the ancient lake.

3.
Pharmaceutical Technology ; 45(11):34-40, 2021.
Article in English | EMBASE | ID: covidwho-1935337
4.
2022 IEEE International Symposium on Olfaction and Electronic Nose, ISOEN 2022 ; 2022.
Article in English | Scopus | ID: covidwho-1922718

ABSTRACT

Volatile organic compounds (VOCs) of urine samples of Covid-19 patients and healthy controls have been collected and analyzed with a gas chromatograph-mass spectrometer (GC-MS) and the actual version of the Tor Vergata electronic nose (E-Nose). This untargeted metabolic investigation leads to a set of 5 discriminative VOCs with 84.38%, 94.44%, 71.43% for accuracy, sensitivity, and specificity. The E- Nose has sniffed Covid-19 subjects at 92.1 %, 100%, 85%, as the accuracy, sensitivity, and specificity respectively. © 2022 IEEE.

5.
Clinica Chimica Acta ; 530:S20, 2022.
Article in English | EMBASE | ID: covidwho-1885649

ABSTRACT

Background-aim: With recent emergences in new infectious diseases and their variants, there is a need to develop a faster and more specific analytical tool to detect different respiratory infectious diseases such as SARS-CoV-2 or influenza viruses. Not only their symptoms are similar at early stages, but also, they are both enveloped viruses with several common biological properties, often leading to challenges in disease identification. Among different viral components, nucleocapsid protein or nucleoprotein (NP) is highly conserved, less post-translational modifications possessed, and mostly specific for each infectious disease virus types. Therefore, targeting NP could be more advantageous to the method development, achieving much simpler and robust method with minimal subsequent modifications. This study describes a targeted approach for simultaneous detection of NPs from different respiratory infectious diseases using immunoprecipitation (IP) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Multiple viruses, SARS-CoV-2, influenza virus A and B types, respiratory syncytial virus, and human coronavirus (HCoV-229E), were selected to show that this method can distinguish different disease viruses. Methods: Sample collected via nasopharyngeal swabs in viral transport media was directly subjected to IP using Thermo Scientific™ Pierce™ MS-Compatible IP Kit (Streptavidin). The IP purified samples were then digested using SMART Digest™ Trypsin Kits and analyzed by Thermo Scientific™ Vanquish™ MD HPLC system hyphenated to Thermo Scientific™ TSQ Altis MD mass spectrometer. Data processing was performed using TraceFinder™ LDT software 1.0. Results: Combining IP and LC-MS/MS resulted in a highly targeted approach with the high sensitivity and specificity. The method detected sub tens to hundreds amol of peptides on LC column. Also, it simplified the overall sample preparation process eliminating prior protein precipitation and post sample clean-up. Since the NPs mostly remain unchanged or less modified regardless of variants, the method doesn’t need tremendous alterations once established. Conclusions: This targeted approach can be applied to other enveloped viruses’ detection. Automated IP method is available with KingFisher system so it could lead to a faster turn-around time and higher throughput of the method.

6.
Biochemical and Cellular Archives ; 21(2):4847-4854, 2021.
Article in English | EMBASE | ID: covidwho-1614921

ABSTRACT

Fused pyrimidines gain an increasing interest as being a precursor for biologically active new compounds. The fused pyrimidine derivatives (1-5) were prepared by condensation of the 1,8-diamine naphthalene with some medical compounds in the presence of ammonium chloride as a dehydration agent for the water molecule and toluene as a solvent. Mannich base compounds (6-10) were prepared by reacting pyrimidines (1-5) with formaldehyde and 4-methoxyaniline. A microwave method was used in preparing the compounds. The prepared compounds were characterized by physical methods, through melting points and color, as well as by spectroscopic methods such as FT-IR and 1H-NMR. The purity of the prepared compounds was evaluated using TLC. The bioactivity of these compounds was tested against two types of bacteria, i.e. Escherichia coli and Staphylococcus aureus. The results of bioactivity showed an antibacterial activity compared to the standard drugs Cephalexin and Amoxicillin. The stability of selected compounds was evaluated by laser irradiation for (10, 20, 30, 40) seconds, and was found to be stable and did not decompose with a 30 seconds exposure. On the other hand, their color was changed at 40 seconds of exposure. Molecular docking studies were conducted to examine how some of the synthesized compounds bind to the putative target, SARS COV2 RNA-dependent RNA polymerase. The study concluded that some of the prepared compounds showed promising antibacterial and antiviral bioactivities. Further in vitro and in vivo toxicological and pharmacological studies are required to evaluate the possibility of using these compounds as a medicine.

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