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1.
International Journal of Innovation and Applied Studies ; 36(1):67-77, 2022.
Article in French | ProQuest Central | ID: covidwho-1843062

ABSTRACT

A multicenter cross-sectional study was conducted, between May and July 2021 before any anti-Covid-19 vaccination program implementation, among 720 staff working in six hospitals in the province of North Kivu in the eastern Democratic Republic of Congo. The aim of the study was to determine the seroprevalence of anti-SARS-CoV-2 antibodies. Individual data on socio-demographic and professional parameters and wearing mask were collected on the basis of a standard form. A blood sample was taken for qualitative determination of anti-SARS-CoV-2 antibodies using immuno-chromatographic "Panbio COVID-19 IgG/IgM Device (25T)" kits. The overall IgG/IgM sero-prevalence was 32.9% (n = 720). This seroprevalence among hospital staff was not significantly associated with their age, gender, professional category, department to which they were assigned in the hospital, or location in a rural or urban setting of their hospitals, nor to the systematic wearing of masks. Among hospital staff who reported contact with a Covid-19 patient, seroprevalence was twice as high at service on the workplace 32.6% (n = 282) [(PR (95% CI)], [2.30 (1.46 - 2.95)] (p = 0.001). In conclusion, the anti-SARS-CoV-2 antibodies seropositivity level among service providers in hospitals in North Kivu province in Eastern DRC is high and that contamination seems more than half as frequent in a professional hospital environment as at the level of the family unit.Alternate :Une enquête transversale multicentrique a été menée, entre mai et juillet 2021 avant toute mise en oeuvre du programme de vaccination anti-Covid-19, auprès de 720 personnels oeuvrant dans six hôpitaux de la province du Nord Kivu dans la partie Est de la République Démocratique du Congo. L'objectif poursuivi était de déterminer la séroprévalence des anticorps anti-SARS-CoV-2. Les données individuelles sur les paramètres sociodémographiques, professionnels et le port du masque ont été recueillies sur base d'un formulaire standard. Un échantillon de sang était prélevé pour la recherche qualitative des anticorps anti-SARS-CoV-2 par la méthode immuno-chromatographique rapide en utilisant les kits « Panbio COVID-19 IgG/IgM Device (25T) ». La séroprévalence globale IgG/IgM était de 32,9% (n = 720). Cette séroprévalence parmi les personnels des hôpitaux n'était significativement associée ni à leur âge, ni à leur sexe, ni à leur catégorie professionnelle, ni à leur service d'affectation dans l'hôpital, ni à la localisation dans un milieu rural ou urbain de leurs hôpitaux, ni au port systématique de masque. Parmi les personnels des hôpitaux ayant signalé un contact avec un malade Covid-19, la séroprévalence était 2 fois plus élevée lorsque le contact était survenu au domicile 75,0% (n= 16) comparée à ceux dont le contact était déclaré au service sur le lieu de travail 32,6% (n = 282) [(RP (IC à 95%)], [2,30 (1,46 - 2,95)] (p = 0,001). En conclusion, le niveau de séropositivité des anticorps anti-SARS-CoV-2 parmi les prestataires des hôpitaux de la province du Nord Kivu en RDC est élevé et que la contamination semble plus de deux fois moins fréquente en milieu professionnel hospitalier qu'au niveau du noyau familial.

2.
Applied Sciences ; 12(9):4538, 2022.
Article in English | ProQuest Central | ID: covidwho-1837865

ABSTRACT

Airborne pollutant transport in an aircraft cabin is greatly affected by the created airflow. The seat layout can impact the flow and thus the pollutant transport. Most studies have adopted symmetric upright seats for simplicity. The influence of seat inclination and seat misalignment on airflow and pollutant transport is still unclear. This investigation adopted a validated computational fluid dynamics (CFD) method to study the airflow and airborne pollutant distribution in a single-aisle cabin with seven rows of seats. The pollutant was assumed to be released from a passenger seated in the middle of three adjacent seats. A total of five different seat layouts were considered, including all of the upright seats, the inclination of three adjacent seats, the inclination of all of the seats in half a cabin, the inclination of all of the seats in a whole cabin, and the misalignment seat rows across the aisle. The flows in both the cross and longitudinal sections were compared. The pollutant concentrations in the respiratory zone of the passengers in different seats were adopted to evaluate the cross-contamination. The results revealed that the symmetric seat layout aids to circumscribe the released pollutant in a small region and reduces the cross-contamination either by maintaining the upright seats or inclining all of the seats. Contrarily, any inclination of seats or a misalignment of seat rows should be avoided during the pandemic since an asymmetric seat layout would generate asymmetric flow and strengthen the spreading of pollutants.

3.
Insights into Imaging ; 13(1), 2022.
Article in English | ProQuest Central | ID: covidwho-1837326

ABSTRACT

BackgroundDuring the current severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) pandemic, computed tomography (CT) has become widely used in patients with suspected or known coronavirus disease 2019 (COVID-19). This prospective observational study in 28 invasively ventilated and 18 non-invasively ventilated patients with confirmed SARS-CoV-2 contamination aims at investigating SARS-CoV-2 contamination of CT scanner surfaces and its infectiousness.MethodsSwab sampling of the CT table and gantry before and after CT examinations was performed. Additionally, the CT ventilation system air grid was wiped off after each examination. Real-time reverse-transcription polymerase chain reaction (RT-PCR) for SARS-CoV-2 RNA (ribonucleic acid) and viral cell culture were performed in the virology core lab.ResultsAfter examination of non-invasively ventilated or non-ventilated patients, SARS-CoV-2 RNA was found in 11.1% (4/36) on patient near surfaces (CT table and gantry) and in 16.7% (3/18) on the CT air grid respectively after examination of invasively ventilated patients in 5.4% (3/56) on CT table and gantry and 7.1% (2/28) on the CT air grid. Surface contamination was more common in non-invasively ventilated or non-ventilated patients with a high viral load who were actively coughing. RT-PCR cycle threshold (Ct) was high (35.96–39.31) in all positive samples and no positive viral cell culture was found.ConclusionOur study suggests that CT scanner surface contamination with SARS-CoV-2 is considerable and more common after examination of non-invasively ventilated or non-ventilated patients compared to invasively ventilated patients. However, no viral cell culture positivity was found, hence the infectious potential seems low.

4.
Int J Infect Dis ; 118: 211-213, 2022 May.
Article in English | MEDLINE | ID: covidwho-1838859

ABSTRACT

SARS-CoV-2 Omicron strain emergence raised concerns that its enhanced infectivity is partly due to altered spread/contamination modalities. We therefore sampled high-contact surfaces and air in close proximity to patients who were verified as infected with the Omicron strain, using identical protocols applied to sample patients positive to the original or Alpha strains. Cumulatively, for all 3 strains, viral RNA was detected in 90 of 168 surfaces and 6 of 49 air samples (mean cycle threshold [Ct]=35.2±2.5). No infective virus was identified. No significant differences in prevalence were found between strains.


Subject(s)
COVID-19 , SARS-CoV-2 , Humans , RNA, Viral/genetics , Specimen Handling
5.
Pollutants ; 1(2):95, 2021.
Article in English | ProQuest Central | ID: covidwho-1834869

ABSTRACT

Microplastics are discharged into the environment through human activities and are persistent in the environment. With the prevalent use of plastic-based personal protective equipment in the prevention of the spread of the COVID-19 virus, the concentration of microplastics in the environment is envisaged to increase. Potential ecological and health risks emanate from their potential to adsorb and transport toxic chemicals, and ease of absorption into the cells of living organisms and interfering with physiological processes. This review (1) discusses sources and pathways through which microplastics enter the environment, (2) evaluates the fate and behavior of microplastics, (3) discusses microplastics in African aquatic systems, and (4) identifies research gaps and recommends remediation strategies. Importantly, while there is significant microplastics pollution in the aquatic environment, pollution in terrestrial systems are not widely studied. Besides, there is a dearth of information on microplastics in African aquatic systems. The paper recommends that the governments and non-governmental organizations should fund research to address knowledge gaps, which include: (1) the environmental fate of microplastics, (2) conducting toxicological studies under environmentally relevant conditions, (3) investigating toxicity mechanisms to biota, and developing mitigation measures to safeguard human health, and (4) investigating pollutants transported by microplastics. Moreover, regulatory measures, along with the circular economy strategies, may help reduce microplastic pollution.

6.
Encyclopedia ; 1(2):401, 2021.
Article in English | ProQuest Central | ID: covidwho-1834746

ABSTRACT

DefinitionDuring the SARS-CoV-2 pandemic, the self-service restaurant sector, as well as other types of food services, are facing an unprecedented crisis needing to adapt their service to avoid closing their doors. With varied and quick meals, the self-service buffet is one of the most important types of outside services. However, the type of service where the clients follow a line on the buffet and serve their meals has impaired traditional restaurant operation during the SARS-CoV-2 pandemic and, perhaps, after it. In this sense, this study presents an overview of the self-service buffet restaurant operational system in the context of the SARS-CoV-2 pandemic.

7.
IOP Conference Series. Earth and Environmental Science ; 1015(1):011001, 2022.
Article in English | ProQuest Central | ID: covidwho-1830924

ABSTRACT

CITIES International Conference is an annual international conference continually held by the Urban and Planning Regional Department of Institut Teknologi Sepuluh Nopember (ITS Surabaya). With its debut conference in 2005, CITIES was and has been designated as a vessel for researchers, academics, and practitioners to present, disseminate, and discuss research results and scientific analyses in urban and regional research areas. Through the dedicated endeavours of every party, CITIES, which was once national-scoped in its early years, has transformed into an international-scoped conference since 2013. Thus, the diversity of speakers and participants has expanded to worldwide audience, be it from the Asian, European, Australian, or American Continent.CITIES International Conference 2021 will bring the theme “Post-COVID Cities: A Paradigm Shift?”. This theme was chosen due to the fact that urban areas become the most susceptible towards the transmission of SARS-CoV-2. The COVID-19 pandemic has brought forth both various impacts to urban development which are classified into four major themes: environmental quality, socio-economic structure, governance, and urban transportation & design. Such rapid and huge shifts also encourage researchers in the planning field to reflect and simultaneously question fundamental points, such as the efficacy of planning approaches, roles, functions, and models so long adopted to respond and manage critical health situations due to the COVID-19 pandemic. The urban medium no longer facilitates planning and management within the constraints of business as usual. There is an emergence of necessity that cities be smarter, more inclusive, resilient, and sustainable.With said necessity, CITIES International Conference 2021 is designated to become a meeting place for stakeholders, be it academia, researchers, practitioners, and bureaucrats within and beyond national boundaries, to disseminate research results, practical experiences in local, national, and global levels, as wells as share insights and recommendations regarding post-COVID-19 pandemic plans and management.CITIES International Conference 2021 was held virtually using the zoom meeting platform due to COVID-19 outbreaks and travel restriction due to the high contamination number. CITIES International Conference 2021 was held on two days, on 20th October 2021, comprising keynote speeches and plenary sessions, and 21st October 2021, comprising panel discussion sessions.List of Sponsor Funding Acknowledgements, List Of Committees, Conference Photographs are available in this pdf.

8.
Front Cell Infect Microbiol ; 12: 856553, 2022.
Article in English | MEDLINE | ID: covidwho-1834360

ABSTRACT

Loop-mediated isothermal amplification (LAMP) is being used as a robust rapid diagnostic tool to prevent the transmission of infectious diseases. However, carryover contamination of LAMP-amplified products originating from previous tests has been a problem in LAMP-based bio-analytical assays. In this study, we developed a Cod-uracil-DNA-glycosylase real-time reverse transcriptase LAMP assay (Cod-UNG-rRT-LAMP) for the elimination of carryover contamination and the rapid detection of SARS-CoV-2 in point-of-care (POC) testing. Using the Cod-UNG-rRT-LAMP assay, the SARS-CoV-2 virus could be detected as low as 2 copies/µl (8 copies/reaction) within 45 min of amplification and 2.63 ± 0.17 pg (equivalent to 2.296 × 109 copies) of contaminants per reaction could be eliminated. Analysis of clinical SARS-CoV-2 samples using the Cod-UNG-rRT-LAMP assay showed an excellent agreement with a relative accuracy of 98.2%, sensitivity of 97.1%, and specificity of 95.2% in comparison to rRT-PCR. The results obtained in this study clearly demonstrate the feasibility of the use of the Cod-UNG-rRT-LAMP assay for applications toward the POC diagnosis of SARS-CoV-2 and on-site testing of other pathogens.


Subject(s)
COVID-19 , SARS-CoV-2 , COVID-19/diagnosis , Humans , Molecular Diagnostic Techniques/methods , Nucleic Acid Amplification Techniques/methods , Point-of-Care Testing , RNA, Viral/analysis , RNA, Viral/genetics , RNA-Directed DNA Polymerase , SARS-CoV-2/genetics , Sensitivity and Specificity
9.
Internet of Things ; : 27-41, 2022.
Article in English | Scopus | ID: covidwho-1826225

ABSTRACT

Bioaerosols, in addition to common gaseous or particulate pollutants, are also important air pollutants that deteriorate indoor air quality. Bioaerosols are the airborne particles present as or originating from living organisms such as fungi, bacteria, and viruses and include toxins, fragments, or waste product from various organisms. In this chapter, the characteristics of indoor bioaerosols are provided. The common type and sources of bioaerosols are summarized. These biological pollutants are commonly generated both by the activities and behaviors of the occupants, and by housing materials and substances that penetrate from the outdoor environment. Fungi and bacteria are the most common bioaerosols present in the indoor environment. After exposure, occupants may experience adverse health outcomes such as infection or allergy. If the indoor environment is severely contaminated, as observed in many places during the COVID-19 pandemic, especially in public areas, a large number of people may be affected by contamination. This chapter also summarizes monitoring and assessment technologies. The monitoring procedure can be chosen and performed according to the objective of the assessment. Advance technologies such as real-time sensor monitoring, Internet of Things, and artificial intelligence have been integrated, but their use for bioaerosols monitoring is still limited as compared to their use for other types of indoor air pollutants. Effective control strategies to reduce the contamination of indoor bioaerosols are also provided in this chapter that could benefit occupants to reduce the contamination and minimize exposure. © 2022, The Author(s), under exclusive license to Springer Nature Switzerland AG.

10.
Pakistan Journal of Medical and Health Sciences ; 16(3):837-839, 2022.
Article in English | EMBASE | ID: covidwho-1822799

ABSTRACT

Aim: The knowledge of viral characteristics in addition immune reply to severe respiratory disorder (Sars Syndrome Coronavirus 2 (SARS-CoV-2) contamination still has significant gaps. Methods: In a retrospective longitudinal cohort analysis of 140 cases having PCR-established SARS-CoV-2 disease, researchers analyzed those parameters and demonstrated their correlation with symptom manifestations (mean age, 44 years;54 percent male;48 percent through comorbidities). Breathing models (n = 76) remained obtained for viral culture, serum specimens (n = 32) for IgM/IgG levels, and plasma samples (n = 82) for inflammatory cytokines and chemokines. The illness burden remained connected to the findings of viral culture, serologic tests, also immunological markers. Results: Fifty-eight (58%) cases established viral pneumonia, including 22 (18%) requiring supplementary oxygen and 14 (11%) requiring invasive mechanical ventilation. Twenty of the 77 individuals were positive for viral culture from respiratory samples (24 percent). When the PCR cycle threshold (Ct) value remained more than 31 or greater than 15 days following indication onset, no virus was recovered. Seroconversion happened at a median (IQR) of 13.6 (10-20) days for IgM and 16.1 (14-22) days for IgG;56/63 patients (88.2 percent) seroconverted on day 15 or later. Health hazard appeared linked to quicker seroconversion as well as greater peak IgM and IgG levels. Conclusion: Researchers discovered that viral viability significantly related having such a lower PCR Ct charge in the initial stages of disease. The seriousness of the illness was linked to a greater antibody level. Overcharged pro-inflammatory immune markers provide marks for host-directed immunotherapy, that would have been investigated in randomized precise studies.

11.
Microbiology and Biotechnology Letters ; 50(1):95-101, 2022.
Article in Korean | EMBASE | ID: covidwho-1819165

ABSTRACT

Due to the pandemic caused by COVID-19, the demand for face masks is soaring and has often caused a shortage. The aim of this study was to evaluate the effect of ultraviolet (UV) and drying treatments on microbial contaminants in facial masks. To conduct this study, standard procedures were designed to develop samples contaminated by the control bacteria Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The contamination level of the standard samples was approximately 6.30 × 106 CFU/ml, and the UV light treatment was performed 1, 3, 5, and 7 times. To evaluate the effect of the UV and drying treatments, the masks were first treated with UV 1, 2, and 3 times, followed by the drying process. As a result, the mask contaminated with E. coli and P. aeruginosa showed a bacterial rate of approximately 99.9% after 1 UV irradiation, and in the case of the S. aureus-contaminated mask, it exhibited a bactericidal rate of approximately 99.9% after 7 UV irradiations. However, when the drying process was included after UV irradiation, all the samples contaminated with E. coli, S. aureus, and P. aeruginosa showed a bactericidal rate of 99.9% or more. The results of this study suggest that UV and drying treatments can effectively reduce the bacterial contaminants in facial masks. In addition, these results provide fundamental data and appropriate sterilization methods for reusing masks.

12.
Clin Infect Dis ; 74(8): 1485-1488, 2022 Apr 28.
Article in English | MEDLINE | ID: covidwho-1816023

ABSTRACT

A false-positive severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse-transcription polymerase chain reaction result can lead to unnecessary public health measures. We report 2 individuals whose respiratory specimens were contaminated by an inactivated SARS-CoV-2 vaccine strain (CoronaVac), likely at vaccination premises. Incidentally, whole genome sequencing of CoronaVac showed adaptive deletions on the spike protein, which do not result in observable changes of antigenicity.


Subject(s)
COVID-19 Vaccines , COVID-19 , COVID-19/prevention & control , Humans , SARS-CoV-2/genetics , Vaccination
13.
Journal of Aerosol Medicine and Pulmonary Drug Delivery ; 35(2):A3, 2022.
Article in English | EMBASE | ID: covidwho-1815949

ABSTRACT

Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for the global pandemic of coronavirus disease 2019 (COVID-19), has afflicted more than 245 million people worldwide and caused more than 5 million deaths (1). COVID-19 primarily affects the respiratory tract and infected persons generate respiratory droplets and aerosols containing the virus that transmit the infection to susceptible hosts (2). Patients with COVID- 19 need inhaled therapies, either for pre-existing respiratory diseases or because of new onset respiratory distress and hypoxemia in patients with no previous pulmonary problems. Aerosolized therapies increase particle concentrations in the vicinity of patients receiving such treatments (3, 4). Inhalers (pMDIs, DPIs and SMIs) have a very low risk of contamination and the risk of spreading infection with those devices is largely due to ''bioaerosols'' generated by the patient during breathing, speaking, coughing or sneezing (2,5,6). In contrast, nebulizers, especially those that are operated continuously, release ''fugitive emissions'' that could remain in the indoor environment.

14.
Nigerian Agricultural Journal ; 52(2):356-366, 2021.
Article in English | CAB Abstracts | ID: covidwho-1812919

ABSTRACT

There is an increasing universal awareness of environmental problems arising as a result of COVID- 19 pandemic and pollution especially in Nigeria. Among the source of this problem is effluent discharge from industries, particularly hospitals in arable farmlands and environs. Two outstanding hospital were purposively selected;Madonna Catholic Hospital and Abia Specialist Hospital in Umuahia, Abia State. Their wastewater samples were collected from three different wards;maternity, general private, and general out-patients department (GOPD) wards. Results obtained show significant variation in physiochemical properties in some wards and heavy metals across all wards. Seven bacteria species;Staphylococcus aureus, Escherichia coli, Klesbsiella pneumonia, Pseudomonas aeruginosa, Proteus vulgaris, Bacteriod sp and Streptococcus pyogenes and one fungi specie- Candida albican were recorded from the samples. The bacterial load in Madonna ranged from 209.04 to 232.95cfu/ml in January, February, and March each and was statistically the same in the three wards (p>0.05). Fungi load ranged from 1.58 to 2.35cfu/ml in January, February, and March each and also significantly different at (p>0.05). The frequency of microbial characteristics isolated in the two hospital wastewater ranged from 33 to 100% with 100% of Staphylococcus aureus and Escherichia coli in all the wards, while other species varied significantly between 67 and 33% each. The results of the isolated bacteria from hospital wastewater showed resistivity to the tested antibiotics, and as therapeutic agents. Therefore, results call for need for urgent attention to be given to the discharge of wastewater from hospitals to ensure that food production around the environment is not contaminated.

15.
J Food Prot ; 84(7): 1239-1251, 2021 Jul 01.
Article in English | MEDLINE | ID: covidwho-1810920

ABSTRACT

ABSTRACT: Cross-contamination of raw food to other surfaces, hands, and foods is a serious issue in food service. With individuals eating more meals away from home, contracting a foodborne illness from a food service establishment is an increasing concern. However, most studies have concentrated on hands or food contact surfaces and neglected atypical and unusual surfaces (surfaces that are not typically identified as a source of cross-contamination) and venues. This review was conducted to identify atypically cross-contaminated surfaces and atypical venues where cross-contamination could occur that have not been examined thoroughly in the literature. Most surfaces that could be at risk for cross-contamination are frequently touched, are rarely cleaned and sanitized, and can support the persistence and/or growth of foodborne pathogens. These surfaces include menus, spice and condiment containers, aprons and coveralls, mobile devices and tablets, and money. Venues that are explored, such as temporary events, mobile vendors, and markets, are usually limited in space or infrastructure, have low compliance with proper hand washing, and provide the opportunity for raw and ready-to-eat foods to come into contact with one another. These factors create an environment in which cross-contamination can occur and potentially impact food safety. A more comprehensive cleaning and sanitizing regime encompassing these surfaces and venues could help mitigate cross-contamination. This review highlights key surfaces and venues that have the potential to be cross-contaminated and have been underestimated or not fully investigated. These knowledge gaps indicate where further work is needed to fully understand the role of these surfaces and venues in cross-contamination and how it can be prevented.


Subject(s)
Food Services , Foodborne Diseases , Food Contamination/analysis , Food Handling , Food Microbiology , Food Safety , Hand , Hand Disinfection , Humans
16.
Sustainability ; 14(8):4713, 2022.
Article in English | ProQuest Central | ID: covidwho-1810157

ABSTRACT

The effect of substrate type and cultivation site in the urban fabric on growth, nutrient content and potentially toxic element (PTE) accumulation in tissues of the halophyte Crithmum maritimum was studied. Plantlets were cultivated for twelve months in containers with a green-roof infrastructure fitted and placed either on an urban second-floor roof or on ground level by the side of a moderate-traffic street. Two substrate types were used;one comprising grape marc compost, perlite and pumice (3:3:4, v/v) and one composed of grape marc compost, perlite, pumice and soil (3:3:2:2, v/v), with 10 cm depth. Plants grew well on both sites, although aboveground growth parameters and nutrient content in leaves were greater at street level. Both cultivation site and substrate type affected heavy-metal accumulation in plant tissues. Cu, Ni and Fe concentrations in leaves and Pb in roots were higher in street-level-grown plants compared to the roof-grown plants, and concentrations of Cu and Mn in leaves and Fe in both leaves and roots were lower in the soilless substrate compared to the soil-substrate, making the soilless type preferable in the interest of both safer produce for human consumption and lower construction weight in the case of green-roof cultivation.

17.
2nd International Conference on Artificial Intelligence and Smart Energy, ICAIS 2022 ; : 1714-1719, 2022.
Article in English | Scopus | ID: covidwho-1806905

ABSTRACT

COVID-19 is an infectious disease that causes serious pandemic. There are many means to contradict the outspread of the microbe and one such way is to sterilize the hands by washing with soap for 20 seconds. Despite washing hands regularly, there is a tendency to touch the contaminated surface in the tap and door handles. In order to make the hands contamination-free before entering the premise, a solution that a person will be allowed to enter only after hand washing has been implemented. Having an automatic faucet and door which will be controlled using the micro controller Arduino Nano wherein various sensors like IR sensor and other devices like servo motor, relay module and LCD display will be interfaced with the micro controller has been proposed. An IR sensor will be placed at the door that senses whether the person has washed his/her hands or not and grant access for the entry. Another IR sensor will be placed at the faucet and it will sense the person's hand and control the water flow accordingly. The proposed system will ensure whether people have washed their hands before entering their premises and grant access accordingly. © 2022 IEEE.

18.
Int J Infect Dis ; 120: 132-134, 2022 Jul.
Article in English | MEDLINE | ID: covidwho-1804266

ABSTRACT

OBJECTIVES: Preventing reverse transcription loop-mediated isothermal amplification (RT-LAMP) carryover contamination could be solved by adding deoxyuridine triphosphate (dUTP) and uracil-DNA glycosylase (UDG) into the reaction master mix. METHODS: RNA was extracted from nasopharyngeal swab samples by a simple RNA extraction method. RESULTS: Testing of 77 samples demonstrated 91.2% sensitivity (95% confidence interval [CI]: 78-98.2%) and 100% specificity (95% confidence interval: 92-100%) using UDG RT-LAMP. CONCLUSION: This colorimetric UDG RT-LAMP is a simple-to-use, fast, and easy-to-interpret method, which could serve as an alternative for diagnosis of SARS-CoV-2 infection, especially in remote hospitals and laboratories with under-equipped medical facilities.

19.
IOP Conference Series: Earth and Environmental Science ; 1016(1):011001, 2022.
Article in English | ProQuest Central | ID: covidwho-1806214

ABSTRACT

Intro and GoalCovid-19 pandemic teaches us that climate change impact sometimes incredibly unpredictable. Not only the increase of heat exposure or natural hazard due to extreme weather, but the emergence of new species or the mutation of microorganism due to the changing ecology may harmful for human beings and another organism. This anthropological climate change phenomenon has negatively altered the most fundamental natural resources for living organism: air, water, and soil. Air has been polluted and warming due to human activities. The quantity of freshwater keeps on depleting, while the ground water recharge intervened with chemical and biological contamination. The sustainability of soil as the natural planting media is under threat due to land use conversion and soil quality degradation. Water acidification and temperature increase have been proved to change the salinity of seawater. Those climate change sensitive exposure may affect the immune system of the living things to survive. Those conditions grow the concern for the health status of humans, crops, farmed and wild animals.Preparing immunity on living organism, especially human is a must to maintain our existence. Vaccine is vital to protect human health from emergence virus and disease which may affected by climate change. But does vaccine can against climate change itself? The 7th International Conference on Climate Change (ICCC) is focusing the discussion on the Covid-19 vaccine roles to against the climate change. Focusing and improving the climatic resilience strategies on immune system will be of considerably important to overcome the key climate change sensitive pathways, as well as to support the achievements some global goals in the Sustainable Development Goals (SDG).The 7th ICCC 2021 is organized by Dept. of Master Program of Environmental Science, Post Graduate School of Sebelas Maret University (Surakarta, Indonesia), collaborating with The United Graduate School of Agriculture Science (UGSAS), and Gifu University (Japan).This conference aims to accommodate the new related inspiration and innovation about how to minimize the climate change impact at present. Especially at the 7th ICCC 2021, the purpose is to formulate a comprehensive and efficient strategies on how to increase the effectiveness of Covid-19 Vaccine against the climate change. The e-poster of call for papers presented in Figure 1.List of titles Invited Speakers and Guest Editors, Virtual Conference Disclaimer, Date of conference, Location of organizer and Conference model, Location of participants and overall participant number, Committee List, The success of delivery of the conference, Chairman are available in this Pdf.

20.
Critical Care ; 26(SUPPL 1), 2022.
Article in English | EMBASE | ID: covidwho-1793853

ABSTRACT

Introduction: The COVID-19 epidemic has been developing in Tunisia officially since March 2, 2020. Since the spread of the epidemic, the governorate of Mahdia has experienced 23,271 cases of contamination including 673 deaths and 20,739 cases of recovery. The aim of this study is to describe the epidemiological and clinical characteristics of patients with COVID-19 who have been hospitalized in the emergency department of Mahdia. Methods: This is a retrospective study, carried out in the Emergency Department of Mahdia over a period of 12 months extending from September 2020 until August 2021. We included patients over 18 years-old hospitalized with COVID-19 lung disease confirmed either by RT-PCR or rapid antigen testing. For all patients included, demographic, clinical, biological and therapeutic data were collected as well as the patient's outcome. Results: A total of 976 patients were included, the mean age was 67. Patients over 70 years of age accounted for 40%. The sex ratio 1.06. 74.2% of patients had at least one comorbidity, including hypertension (52%) and diabetes (46%). The most frequent clinical sign was dyspnea (77%). Initial management included low flow oxygen therapy in 319 patients (33%), high flow oxygen therapy in 426 patients (44%). And 102 patients (10%) received non-invasive ventilation and 13 (1%) received invasive ventilation. Severe forms are frequent (43% of cases). Patients admitted to the emergency department only (360 or 37%), compared to those transferred to intensive care (209, 21%) and to the COVID department (373, 38%), were older [age average respectively: 73 years (SD ± 13);66 (SD ± 13);61 (SD ± 13);p = 0.001] and likely to have at least one comorbidity (79%, 76%, 73% respectively). The case fatality rate was 31.5% in all patients. Conclusions: In this study, our patients were mainly elderly, male, having at least one comorbidity with a high mortality rate, hence the need for a prospective study specifying the predictive factors of mortality.

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