Debilitating landscapes of care and support: envisaging alternative futures

This paper explores the impact of policy changes and budget cuts on services and support faced by people with learning disabilities. Drawing upon collaborative research in England and Scotland and interviews with commissioners and support organisations, we show how landscapes of care and support are unstable and fragmented. We identify how pressures of time, resource and precaritisation in the workforce are creating ‘debilitating landscapes of care' that further erode the capacities of both the people that work in the sector and people with learning disabilities. Some challenges that people with learning disabilities face in this context include finding appropriate local support, narrowing access as a result of reductions in benefit entitlements and identifying quality providers amid a complex array of private and charitable provision. Capacity to cope with these challenges is contingent on access to quality advocacy, supportive family, friendships, productive occupational learning environments and peer support, but these are not always available. The impact of COVID-19 has only served to intensify some of the issues we identify and the urgent need for a response. Our analysis is inspired by Berlant's (2007) conception of ‘slow-death' and Puar's (2017) associated conceptualisation of ‘debility'. © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Urban landscape and climate affect residents' sentiments based on big data

Residents' sentiments are a quantitative indicator of human feelings, which is useful for optimizing urban residential environments. Little is known about the spatiotemporal variations and potential drivers of sentiment based on big data. A total of 221,104 Weibo social media data were used to quantify daily sentiment in the Beijing metropolitan area during the COVID-19 pandemic from January 1, 2021 to March 8, 2022. Deep-learning natural language processing was used to extract this dataset to investigate the spatiotemporal sentiment patterns. The density of roads and buildings, normalized difference vegetation index (NDVI), population, sky visibility factor, daily land surface temperature (LST), daily precipitation, and daily air pollution concentrations (CO, NO2, PM2.5, SO2, and O3) were explored as potential drivers of sentiment. Results show that (1) the holiday sentiment was 1.31% higher than on weekends and 4.61% higher than on weekdays. Extreme precipitation, air pollution, and COVID-19 lockdown measures have reduced sentiment. (2) The sentiment in spring was found to be the highest. The numbers of functional zones with high sentiment values (>0.8) in spring were 13.59%, in summer 34.48%, and in autumn 14.71%. (3) Sentiment was highest under conditions of moderate greenness (0.4<NDVI<0.6) and comfortable daily temperature (25 °C < LST<30 °C). (4) Sentiment was negatively associated with daily air pollutants, such as PM2.5, NO2, and CO. This paper presents the effectiveness of sentiment quantification based on social media data and deep-learning techniques. The results provide practical implications and support decisions for sustainable urban health development.

From garbage to COVID-19: theorizing 'Multilingual Commanding Urgency' in the linguistic landscape

Across the globe signage which conveys directives regarding appropriate behavior in public, such as 'Do Not Enter' signs, is made multilingual in ways that other signage is not. This paper examines two examples of multilingualism in directive signs within Seoul, South Korea in order to theorize what gives rise to multilingualism in directive signage while other signage remains monolingual. Examination of Vietnamese and Arabic on signs prohibiting the illegal disposal of household garbage on side streets in Seoul, and English, Chinese, and Japanese on mask-required due to COVID-19 signs within the Seoul subway system allows for a robust analysis of what shapes the inclusion of additional languages on directive signage. We posit the construction of a differently speaking other who is seen as likely to disobey stated regulations alongside the desire by authorities to minimize the effort required to respond to rule breaking results in a multilingual commanding urgency that shapes multilingualism in directive signage. The concept of multilingual commanding urgency emphasizes the role enforcement practices have in shaping multilingualism, an important development in understanding this form of signage. Multilingual commanding urgency is especially relevant as it shapes signage deployed in emergency contexts such as the COVID-19 pandemic. © 2022 Walter de Gruyter GmbH, Berlin/Boston.

Virtual participatory mapping of nature-based solutions in the Grande de Tárcoles River basin, Costa Rica: Connecting diverse knowledge systems in a context of physical immobility.

Co-design processes are of key importance in planning and implementing Nature-based Solutions (NBS), but require boundary management between diverse holders of local, practical and scientific knowledge. Participatory and map-based planning tools, such as Geodesign workshops, can facilitate such boundary management through mobilising knowledge holders, and translating and negotiating between different perspectives. The COVID-19 pandemic hindered physical mobility, but offered an, albeit unintended, opportunity to explore new ways of virtual mobility for facilitating boundary management in NBS co-design through digital participatory tools. This short communication aims to demonstrate how a spatial planning process for NBS can be facilitated in an online context. We draw on an international case study for co-designing NBS in Costa Rica, conducted during the severe lock-down restriction of the COVID-19 pandemic. This novel approach showcases how physical presence and movement is replaced by virtual mobility enabled through an online geographic map-based environment that allowed participants to communicate their opinions and co-create local and regional NBS actions. The case study included developing and testing a co-design tool to understand and map local perceptions of social-ecological problems, and an actual co-design process for siting NBS options and jointly exploring their implications. We present two levels of the process: 1) the adaptation of the co-design tool, and 2) the use and usefulness of the co-design tool. Our evaluation shows that the tool served its purpose well and provided useful support to local stakeholders. We recommend to test further strategical combinations of in-person and virtual methods in NBS co-design processes to improve NBS planning and implementation.

Understanding the Impact of the COVID-19 Pandemic on the Perception and Use of Urban Green Spaces in Korea.

Faced with the prospect that the impact of the COVID-19 pandemic and climate change will be far-reaching and long-term, the international community is showing interest in urban green space (UGS) and urban green infrastructure utilization as a solution. In this study, we investigated how citizens' perceptions and use of UGS have changed during COVID-19. We also collected their ideas on how UGS can raise its usability. As a result, more people became to realize the importance of UGS. In particular, the urban environmental purification function from UGS was recognized as giving great benefits to respondents. On the other hand, the patterns of UGS use were mixed with decreasing UGS use to maintain social distancing or increasing UGS use to maintain health or substitute other restricted facilities. More than half of respondents had their UGS visit patterns impacted by COVID-19. In particular, the increase rate of UGS use was rather high in the group that seldom used UGS before COVID-19. In addition, they increased the use of UGS to replace other limited facilities, and thus tended to demand an increase in rest facilities. Based on these results, this paper suggested securing social support and sustainability for the policy by reflecting users' demand in landscape planning related to the increase of UGS in the city. This study can contribute to improving the resilience of UGS and the sustainability of urban space planning.

Leveraging Campus Landscapes for Public Health: A Pilot Study to Understand the Psychological Effects of Urban Sheep Grazing on College Campuses.

Since the 1980s, college students in the U.S. have self-reported a decline in their physical and emotional health. With these conditions compounded by the COVID-19 pandemic and its physical distancing restrictions, higher education institutions have an increased responsibility to establish strategic interventions and health-promoting programs for their students. Research collaborations between public health professionals and environmental designers have highlighted the benefits of environmental factors, such as wildlife, street trees, and public parks, on mental health. This pilot project aims to build upon the transdisciplinary dialogue between ecology, design, and public health by examining the social benefits of grazing lawnscape management, which is the practice of using herbivorous livestock to manage turfgrass areas. Through the design of an accessible central campus grazing space for a flock of 25 sheep and use of online questionnaires, a smartphone-based single-item survey, and open-ended feedback given via social media, the UC Davis Sheepmower Project addresses three primary questions: (1) Are there differences in self-reported stress levels and well-being between people who did not watch grazing sheep (no sheepmower group) compared with those who did watch grazing sheep (sheepmower group)? (2) Does holding sheep grazing events create opportunities for education about well-being and engagement with the campus community? (3) Can this type of urban grazing installation ultimately contribute to the overall identity of a college campus? Web-based questionnaire results indicate there is no significant difference in self-reported stress levels between the two groups; however, the moment-in-time smartphone-based single item question suggests that the presence of sheep provides temporary, noticeable relief and enhanced mood for those who observe the animals. Reflections posted on social media suggested that participants found the sheep grazing events fostered feelings of community and placemaking within the campus identity. However, the questionnaire sample indicated the grazing events did not have a significant effect on participants' sense of place or overall campus identity. This transdisciplinary effort breaks down traditionally siloed approaches to human and environmental health and is an example of a whole-systems approach to developing innovative solutions and encouraging applied collective action.

Covid-19 Pandemic and Its Effects on Social Life and Reflections on Spatial Preferences

The Covid-19 pandemic, which emerged in Wuhan city of China's Hubei province in December 2019, affected the whole world in a short period of 3 months. The Covid-19 outbreak, which was declared a pandemic by the World Health Organization as of March 12, 2020;was imposed significant restrictions on the use of open spaces, which adversely affected the daily life of individuals physically, mentally and socially. Apart from the health problems experienced by people, it has also created many spatial choices and changes. New preferences, where social distance is at the forefront, have started new venue organizations along with new requests. Although the changes experienced with the Covid-19 pandemic may seem negative, they contain opportunities that allow change and development. Making people's living environments more functional, remembering the importance of nature and reviewing social relations can be evaluated in this context. Looking from history to the present, the Covid-19 pandemic is not the first and will not be the last. For this reason, the study aims to investigate the change in the spatial preferences in the society with the pandemic and to create an idea for the next pandemics. In this context, 289 people were reached by using the online survey method and various questions were asked. Various results were obtained and interpreted by performing variance analysis, factor analysis, correlation analysis, crosstabs test and frequency analysis (P<0.01) on the obtained data. As a result of the study, from the statistical data, it has been observed that 'people are more oriented to nature' due to the Covid-19 pandemic and that open green spaces suitable for social distance are sought in spatial preferences. These findings shed new light on the value of urban nature as resilience infrastructure during a time of crisis.

The Innerworkings of Digital Storytelling

Traditionally, storytelling was used for entertainment and the transfer of know-how. The advent of digital media gave rise to new possibilities for telling stories. When the context is, for example, to relay information about how to protect a person from COVID-19, it is referred to as serious storytelling. The main objective of this research was to establish what skills and attributes would be required for someone to autonomously "tell” a serious digital story in a resource constrained environment. A systematic literature review of peer reviewed articles resulted in a knowledge bank of articles. Atlas Ti was used to qualitatively analyse these articles. Even though a resource constrained environment may be a limiting factor for telling a digital story, this research has found that emotional support, digital inclusion, as well as assisting individuals with their devices, can pave the way to autonomous digital storytelling.

Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein.

Worldwide SARS-CoV-2 sequencing efforts track emerging mutations in its spike protein, as well as characteristic mutations in other viral proteins. Besides their epidemiological importance, the observed SARS-CoV-2 sequences present an ensemble of viable protein variants, and thereby a source of information on viral protein structure and function. Charting the mutational landscape of the nucleocapsid (N) protein that facilitates viral assembly, we observe variability exceeding that of the spike protein, with more than 86% of residues that can be substituted, on average by three to four different amino acids. However, mutations exhibit an uneven distribution that tracks known structural features but also reveals highly protected stretches of unknown function. One of these conserved regions is in the central disordered linker proximal to the N-G215C mutation that has become dominant in the Delta variant, outcompeting G215 variants without further spike or N-protein substitutions. Structural models suggest that the G215C mutation stabilizes conserved transient helices in the disordered linker serving as protein-protein interaction interfaces. Comparing Delta variant N-protein to its ancestral version in biophysical experiments, we find a significantly more compact and less disordered structure. N-G215C exhibits substantially stronger self-association, shifting the unliganded protein from a dimeric to a tetrameric oligomeric state, which leads to enhanced coassembly with nucleic acids. This suggests that the sequence variability of N-protein is mirrored by high plasticity of N-protein biophysical properties, which we hypothesize can be exploited by SARS-CoV-2 to achieve greater efficiency of viral assembly, and thereby enhanced infectivity.

SARS-CoV-2 Genome Variations in Viral Shedding of an Immunocompromised Patient with Non-Hodgkin's Lymphoma.

BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causing coronavirus disease 2019 (COVID-19) is the most transmissible ß-coronavirus in history, affecting all population groups. Immunocompromised patients, particularly cancer patients, have been highlighted as a reservoir to promote accumulation of viral mutations throughout persistent infection. CASE PRESENTATION: We aimed to describe the clinical course and SARS-CoV-2 mutation profile for 102 days in an immunocompromised patient with non-Hodgkin's lymphoma and COVID-19. We used RT-qPCR to quantify SARS-CoV-2 viral load over time and whole-virus genome sequencing to identify viral lineage and mutation profile. The patient presented with a persistent infection through 102 days while being treated with cytotoxic chemotherapy for non-Hodgkin's lymphoma and received targeted therapy for COVID-19 with remdesivir and hyperimmune plasma. All sequenced samples belonged to the BA.1.1 lineage. We detected nine amino acid substitutions in five viral genes (Nucleocapsid, ORF1a, ORF1b, ORF13a, and ORF9b), grouped in two clusters: the first cluster with amino acid substitutions only detected on days 39 and 87 of sample collection, and the second cluster with amino acid substitutions only detected on day 95 of sample collection. The Spike gene remained unchanged in all samples. Viral load was dynamic but consistent with the disease flares. CONCLUSIONS: This report shows that the multiple mutations that occur in an immunocompromised patient with persistent COVID-19 could provide information regarding viral evolution and emergence of new SARS-CoV-2 variants.

Manufacture and Characterization of GMP-Compliant SARS-COV-2 Cytotoxic T Lymphocytes.

BACKGROUND: Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 virus-specific cytotoxic T-cell lymphocytes (vCTLs) could provide a promising modality in COVID-19 treatment. We aimed to screen, manufacture, and characterize SARS-CoV-2-vCTLs generated from convalescent COVID-19 donors using the CliniMACS® Cytokine Capture System (CCS). METHODS: Donor screening was done by stimulation of convalescent COVID-19 donor peripheral blood mononuclear cells with viral peptides and identification of IFN-γ+ CD4 and CD8 T-cells using flow cytometry. Clinical-grade SARS-CoV-2-vCTLs were manufactured using the CliniMACS® CCS. The enriched SARS-CoV-2-vCTLs were characterized by T-cell receptor sequencing, mass cytometry, and transcriptome analysis. RESULTS: 93% of convalescent donor blood samples passed the screening criteria for clinical manufacture. Three validation runs resulted in enriched T-cells that were 79% ± 21% IFN-γ+ T-cells. SARS-CoV-2-vCTLs displayed a highly diverse TCR repertoire with enhancement of both memory CD8 and CD4 T-cells, especially in CD8 TEM, CD4 TCM and CD4 TEMRA cell subsets. SARS-CoV-2-vCTLs were polyfunctional with increased gene expression in T-cell function, interleukin, pathogen defense, and tumor necrosis factor superfamily pathways. CONCLUSIONS: Highly functional SARS-CoV-2-vCTLs can be rapidly generated by direct cytokine enrichment (12 hours) from convalescent donors. CLINICAL TRIALS REGISTRATIONS: NCT04896606, NCT03266627, NCT03266640, NCT03266653, NCT04197596.

Incipient Parallel Evolution of SARS-CoV-2 Deltacron Variant in South Brazil.

With the coexistence of multiple lineages and increased international travel, recombination and gene flow are likely to become increasingly important in the adaptive evolution of SARS-CoV-2. These processes could result in genetic introgression and the incipient parallel evolution of multiple recombinant lineages. However, identifying recombinant lineages is challenging, and the true extent of recombinant evolution in SARS-CoV-2 may be underestimated. This study describes the first SARS-CoV-2 Deltacron recombinant case identified in Brazil. We demonstrate that the recombination breakpoint is at the beginning of the Spike gene. The 5' genome portion (circa 22 kb) resembles the AY.101 (Delta), and the 3' genome portion (circa 8 kb nucleotides) is most similar to the BA.1.1 (Omicron). Furthermore, evolutionary genomic analyses indicate that the new strain emerged after a single recombination event between lineages of diverse geographical locations in December 2021 in South Brazil. This Deltacron, AYBA-RS, is one of the dozens of recombinants described in 2022. The submission of only four sequences in the GISAID database suggests that this lineage had a minor epidemiological impact. However, the recent emergence of this and other Deltacron recombinant lineages (XD, XF, and XS) suggests that gene flow and recombination may play an increasingly important role in the COVID-19 pandemic. We explain the evolutionary and population genetic theory that supports this assertion, concluding that this stresses the need for continued genomic surveillance. This monitoring is vital for countries where multiple variants are present, as well as for countries that receive significant inbound international travel.